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Inhibition of Ehrlich ascites carcinoma growth by melatonin:Studies with micro-CT

Oncology Research 2024年第1期32卷 175-185页

作者： seher yilmaz ZÜLEYHA DOĞANYIĞIT MERT OCAK EVRIM SUNA ARIKAN SÖYLEMEZ ASLI OKAN OFLAMAZ SÜMEYYE UÇAR ŞÜKRÜATEŞ AMMAD AHMAD FAROOQI Department of Anatomy Faculty of MedicineYozgat Bozok UniversityYozgatTurkey Department of Mechanical and Aerospace Engineering Case Western Reserve UniversityClevelandOhioUSA Department of Histology and Embriology Faculty of MedicineYozgat Bozok UniversityYozgatTurkey Department of Anatomy Faculty of DentistryAnkara UniversityAnkaraTurkey Department of Medical Biology Faculty of MedicineAfyonkarahisar Health Sciences UniversityAfyonTurkey Department of Anatomy Faculty of MedicineErciyes UniversityKayseriTurkey Department of Molecular Oncology Institute of Biomedical and Genetic Engineering(IBGE)IslamabadPakistan

Melatonin is a versatile indolamine synthesized and secreted by the pineal gland in response to the photoperiodic information received by the retinohypothalamic signaling *** has many benefits,such as organizing circadian rhythms and acting as a powerful *** aimed to show the antitumor effects of melatonin in both in vivo and in vitro models through the mammalian target of rapamycin(mTOR)signaling pathway and the Argyrophilic Nucleolar Regulatory Region(AgNOR),using the Microcomputed Tomography(Micro CT).Ehrlich ascites carcinoma(EAC)cells were administered into the mice by subcutaneous *** with solid tumors were injected intraperitoneally with 50 and 100 mg/kg melatonin for 14 *** measurements for the taken tumors were made with micro-CT imaging,immunohistochemistry(IHC),real-time polymerase chain reaction(PCR)and ***,the tumor tissue volume in the Tumor+100 mg/kg melatonin group was significantly lower than that in the other groups in the data obtained from micro-CT *** the IHC analysis,the groups treated with Tumor+100 mg/kg melatonin were compared when the mTOR signaling pathway and factor 8(F8)expression were compared with the control *** was determined that there was a significant decrease(p<0.05).Significant differences were found in the total AgNOR area/nuclear area(TAA/NA)ratio in the treatment groups(p<0.05).Furthermore,there were significant differences between the amount of mTOR mRNA for the phosphatidylinositol 3-kinase(PI3K),AKT Serine/Threonine Kinase(PKB/AKT)genes(p<0.05).Cell apoptosis was evaluated with Annexin V in an in vitro study with different doses of melatonin;It was observed that 100µg/mL melatonin dose caused an increase in the apoptotic cell *** this study,we have reported anti-tumor effects of melatonin in cell culture studies as well as in mice *** characterization of the melatonin-mediated cancer inhibitory effects will be valuable in advanc

关键词： Signaling pathway Apoptosis Real-time PCR AgNOR

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Association between human leukocyte antigen gene polymorphisms and multiple EPIYA-C repeats in gastrointestinal disorders

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World Journal of Gastroenterology 2020年第32期26卷 4817-4832页

作者： Suat Saribas Suleyman Demiryas Erkan yilmaz Omer Uysal Nuray Kepil Mehmet Demirci Reyhan Caliskan Harika Oyku Dinc seher Akkus Nesrin Gareayaghi Sahra Kirmusaoglu Dogukan Ozbey Hrisi B Tokman Serdar S Koksal Ihsan Tasci Bekir Kocazeybek Department of Medical Microbiology Istanbul University-CerrahpasaCerrahpasa Medical FacultyIstanbul 34098Turkey Department of General Surgery Istanbul University-CerrahpasaCerrahpasa Medical FacultyIstanbul 34098Turkey Department of Organ Transplantation HLA LaboratoryIstanbul University-CerrahpasaCerrahpasa Medical FacultyIstanbul 34098Turkey Deparment of Biostatistics Medical School of Bezmialem Vakif UniversityIstanbul 34093Turkey Department of Pathology Istanbul University-CerrahpasaCerrahpasa Medical FacultyIstanbul 34098Turkey Department of Medical Microbiology Beykent University Medical FacultyIstanbul 34520Turkey Center for Blood Istanbul Sisli Hamidiye Etfal Training and Research HospitalUniversity of Health SciencesIstanbul 34360TurkeyDepartment of Molecular Biology and GeneticsT.C.Halic UniversityFaculty of Arts&SciencesIstanbul 34381Turkey Department of Public Health Istanbul University-CerrahpasaCerrahpasa Medical FacultyIstanbul 34098Turkey

BACKGROUND Polymorphisms of human leukocyte antigen(HLA)genes are suggested to increase the risk of gastric cancer(GC).AIM To investigate the HLA allele frequencies of patients with GC relative to a control group in terms of CagA+multiple(≥2)EPIYA-C *** The patient group comprised 94 patients[44 GC and 50 duodenal ulcer(DU)patients],and the control group comprised 86 individuals[(50 non-ulcer dyspepsia patients and 36 people with asymptomatic Helicobacter pylori(***)].Polymerase chain reaction was performed for the amplification of the *** cagA gene and typing of EPIYA *** sequence-specific oligonucleotide(SSO)typing was performed using Lifecodes SSO typing kits(HLA-A,HLA-B HLA-C,HLA-DRB1,and HLA-DQA1-B1 kits).RESULTS The comparison of GC cases in terms of CagA+multiple(≥2)EPIYA-C repeats showed that only the HLA-DQB1*06 allele[odds ratio(OR):0.37,P=0.036]was significantly lower,but significance was lost after correction(Pc=0.1845).The HLA-DQA1*01 allele had a high ratio in GC cases with multiple EPIYA-C repeats,but this was not significant in the univariate *** compared allele frequencies in the DU cases alone and in GC and DU cases together using the same criterion,and none of the HLA alleles were significantly associated with GC or ***,none of the alleles were detected as independent risk factors after the multivariate *** the other hand,in a multivariate logistic regression with no discriminative criterion,HLA-DQA1*01(OR=1.848),HLA-DQB1*06(OR=1.821)and HLA-A*02(OR=1.579)alleles were detected as independent risk factors for GC and *** None of the HLA alleles were detected as independent risk factors in terms of CagA+multiple EPIYA-C ***,HLA-DQA1*01,HLA-DQB1*0601,and HLA-A*2 were independent risk factors with no criterion in the multivariate *** suggest that the association of these alleles with gastric malignancies is not specifically related to cagA and multiple EPIYA C repeats.

关键词： Human leukocyte antigen Helicobacter pylori Gastric cancer Duodenal ulcer EPIYA CagA

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