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Identif.cation of.proteins involved in lignocellulose degradation using in gel zym.gram.analysis com.ined with m.ss spectroscopy-based peptide analysis of.gut proteins f.om.larval Asian Ionghorned beetles, Anoplophora glabripennis

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Insect Science 2010年第3期17卷 253-264页

作者： scott m. Geib m.ng Tien Kelli Hoover Departm.nts of. Biochem.stry and m.lecular Biology Departm.nts of.Entom.logy The Pennsylvania State University University Park Pennsylvania USA

Enzym. activities toward lignocellulose substrates were analyzed in the gut of.larval Asian longhorned beetle （Anoplophora glabripennis）. Total protein was extracted f.om.gut contents of.wild collected larvae f.om.an invasive population in Worchester, m., USA. f.om.these protein extracts, lignocellulolytic activities were m.asured （β-1,4- endoglucanase,β-1,4-glucosidase and birch wood xylanase）.β-1,4-glucosidase activity was 0.075 μm.l glucose/m. protein per m.n, endoglucanase activity was m.asured at 0.41/zm.l glucose/m. protein per m.n and xylanase activity was 0.058μm.l xylose/m. protein per m.n. To identif. specif.c enzym.s that m.y provide these activities, zym.gram.analysis was perf.rm.d to detect enzym.s active toward carboxym.thyl cellulose （Cm.）, 4-m.thylum.ellif.ryl-β-D-glueopyranoside and birch wood xylan. Three protein bands were f.und to be active toward Cm., three displayed β-1,4-glucosidase, and one displayed xylanase activity. Proteins f.om.active bands f.om.these zym.gram. were then identif.ed by in-gel trypsin digestions f.llowed by peptide identif.cation by m.trix-assisted laser desorption ionization - tim. of.f.ight - tim. of.f.ight m.ss spectrom.try （m.）. A custom.A. glabripennis transcriptom. database was used f.r peptide identif.cation, giving highly signif.cant m.tches in all m. analyses. These m.tches were then searched against the National Center f.r Biotechnology Inf.rm.tion database to provide annotation to the transcripts and provide possible classif.cation. f.om.these analyses, we were able to detect enzym.s active toward cellulose and xylan, and proteins putatively involved in lignocellulose degradation in the gut of.this wood-f.eding insect. f.ture research will be f.cused on characterizing these enzym.s through cloning and expression experim.nts and understanding how the lignocellulose degradation system.f.nctions in the gut of.this insect.

关键词： beta-glucosidase cellulase Ceram.ycidae endoglucanase proteom.cs xylanase

f.st vectorized distance m.trix com.utation f.r m.ltiple sequence alignm.nt on m.lti-cores

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International Journal of.Biom.them.tics 2015年第6期8卷 243-257页

作者： m.ham.ed W. A1-Neam. Naglaa m. Reda f.yed f. m. Ghaleb Departm.nt of.m.them.tics f.culty of.Science AI-Azhar UniversityP. O. Box (11884) Al-Nasr Road Cairo Egypt Education College f.r Girlsm.sul University Mosul Iraq Departm.nt of.m.them.tics Faculty of Science Ain Shams University Cairo Egypt

Although high quality m.ltiple sequence alignm.nt is an essential task in bioinf.rm.- tics, it becom.s a big dilem.a nowadays due to the gigantic explosion in the am.unt of.m.lecular data. The m.st consum.ng tim. and space phase is the distance m.trix com.utation. This paper addresses this issue by proposing a vectorized parallel m.thod that accom.lishes the huge num.er of.sim.larity com.arisons f.ster in less space. Per- f.rm.nce tests on real biological datasets using core-iT show superior results in term. of.tim. and space.

关键词： Bioinf.rm.tics m.ltiple sequence alignm.nt distance m.trix parallel program.ing m.lti-cores.

Evaluation of.stress intensity f.ctors f.r bi-m.terial interf.ce cracks using displacem.nt jum. m.thods

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Acta m.chanica Sinica 2017年第6期33卷 1051-1064页

作者： K. C. Nehar B. E. Hachi f. Cazes m. Haboussi Laboratoire de développem.nt en m.canique et m.tériaux (LDm.) Université de Djelfa PB 3117 Djelfa Algeria Laboratoire des Sciences des Procédés et des m.tériaux (LSPm. UPR 3407 CNRS Université Paris 13 Paris France

The aim.of.the present work is to investigate the num.rical m.deling of.interf.cial cracks that m.y appear at the interf.ce between two isotropic elastic m.terials. The extended f.nite elem.nt m.thod is em.loyed to analyze brittle and bi-m.terial interf.cial f.tigue crack growth by com.uting the m.xed m.de stress intensity f.ctors(SIf.. Three dif.erent approaches are introduced to com.ute the SIf.. In the f.rst one, m.xed m.de SIf.is deduced f.om.the com.utation of.the contour integral as per the classical J-integral m.thod,whereas a displacem.nt m.thod is used to evaluate the SIf.by using either one or two displacem.nt jum.s located along the crack path in the second and third approaches. The displacem.nt jum. m.thod is rather classical f.r m.no-m.terials,but has to our knowledge not been used up to now f.r a bim.terial. Hence, use of.displacem.nt jum. f.r characterizing bi-m.terial cracks constitutes the m.in contribution of.the present study. Several benchm.rk tests including param.tric studies are perf.rm.d to show the ef.ectiveness of.these com.utational m.thodologies f.r SIf.considering static and f.tigue problem. of.bi-m.terial structures. It is f.und that results based on the displacem.nt jum. m.thods are in a very good agreem.nt with those of.exact solutions, such as f.r the J-integral m.thod, but with a larger dom.in of.applicability and a better num.rical ef.iciency(less tim. consum.ng and less spurious boundary ef.ect).

关键词： Bi-m.terial interf.ce crack m.xed m.de stress intensity f.ctor Displacem.nt jum. X-f.m f.tigue crack growth

Indoor and outdoor BTX levels in Barcelona City m.tropolitan area and Catalan rural areas

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Journal of.Environm.ntal Sciences 2008年第9期20卷 1063-1069页

作者： E. Gallego f. X. Roca X. Guardino m. G. Rosell Environm.ntal Centre Laboratory Polytechnic University of Catalonia (LCMA-UPC) National Centre of.Work Conditions (INSHT)

f.ve arom.tic hydrocarbons (benzene, toluene, and three isom.ric xylenes) were m.nitored in indoor and outdoor air of.7 public buildings and 54 private hom.s, located in Barcelona City m.tropolitan area and in several rural areas of.Catalonia. The sam.ling was carried out over f.ur periods: spring-sum.er and winter of.2000, and sum.er and winter of.2001. Passive ORSA 5 Dra¨ger sam.lers were used f.r benzene, toluene, and xylenes (BTX) adsorption. BTX were extracted with carbon disulphide and analysed using ...

关键词： benzene toluene and xylenes (BTX) indoor air outdoor air

Can Quick Release Experim.nts Reveal the m.scle Structure？ A Bionic Approach

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Journal of.Bionic Engineering 2012年第2期9卷 211-223页

作者： D. f. B. Haeuf.e m. Gunther R. Blickhan S. Schm.tt Institute of.Sports and Exercise-Science University of Stuttgart Allmandring 28 D-70569 Stuttgart Germany Stuttgart Research Centre f.r Sim.lation Technology （SRC Sim.ech） University of Stuttgart Pfaffenwaldring 7a D-70569 Stuttgart Germany Institute of.m.tion Science Friedrich-Schiller-University Seidelstraβe 20 D-07749 Jena Germany

The goal of.this study was to understand the m.croscopic m.chanical structure and f.nction of.biological m.scle with respect to its dynam.c role in the contraction. A recently published m.scle m.del, deriving the hyperbolic f.rce-velocity relation f.om.f.rst-order m.chanical principles, predicts dif.erent f.rce-velocity operating points f.r dif.erent load situations. With a new approach, this m.del could be sim.lif.ed and thus, transf.rred into a num.rical sim.lation and a hardware experim.nt. Two types of.quick release experim.nts were perf.rm.d in sim.lation and with the hardware setup, which represent two extrem. cases of.the contraction dynam.cs： against a constant f.rce （isotonic） and against an inertial m.ss. Both experim.nts revealed hyperbolic or hyperbolic-like f.rce-velocity relations. Interestingly, the analytical m.del not only predicts these extrem. cases, but also additionally all contraction states in between. It was possible to validate these predictions with the num.rical m.del and the hardware experim.nt. These results prove that the origin of.the hyperbolic f.rce-velocity relation can be m.chanically explained on a m.croscopic level by the dynam.cal interaction of.three m.chanical elem.nts. The im.lications f.r the interpretation of.biological m.scle experim.nts and the realization of.m.scle-like bionic actuators are discussed.

关键词： f.rce-velocity relation isotonic quick release proof.of.concept artif.cial m.scle

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了不起的盖茨比

Angular Dependence of.Lateral and Levitation f.rces in Asym.etric Sm.ll m.gnet/Superconducting System.

作者： f. scott f.tzgerald[]

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Chinese Physics Letters 2007年第9期24卷 2700-2703页

作者： H. m. Al-Khateeb m. K. Alqadi f. Y. Alzoubi N. Y. Ayoub Departm.nt of.Physics Jordan University of Science and Technology Irbid Jordan School of.Applied Natural Sciences German Jordanian University Amman Jordan

The dipole-dipole interaction m.del is used to calculate the angular dependence of.lateral and levitation f.rces on a sm.ll perm.nent m.gnet and a cylindrical superconductor in the m.issner state lying laterally of. the sym.etric axis of.the cylinder. Under the assum.tion that the lateral displacem.nt of.the m.gnet is sm.ll com.ared with the physical dim.nsions of.the system. we obtain analytical expressions f.r the lateral and levitation f.rces as f.nctions of.geom.trical param.ters of.the superconductor as well as the height, the lateral displacem.nt and the orientation of.m.gnetic m.m.nt of.the m.gnet. The ef.ect of.thickness and radius of.the superconductor on the levitation f.rce is sim.lar to that f.r a sym.etric m.gnet/superconducting cylinder system. but within the range of.lateral displacem.nt. The splitting in the levitation f.rce increases with the increasing angle of.orientation of.the m.gnetic m.m.nt of.the m.gnet. f.r a given lateral displacem.nt of.the m.gnet, the lateral f.rce vanishes when the m.gnetic m.m.nt is perpendicular to the surf.ce of.the superconductor and has a m.xim.m.value when the m.m.nt is parallel to the surf.ce. f.r a given orientation of.the m.gnetic m.m.nt, the lateral f.rce has a linear relationship with the lateral displacem.nt. The stability of.the m.gnet above the superconducting cylinder is discussed in detail.

关键词： THIN SUPERCONDUCTING DISKS m.ISSNER STATE m.GNETIC-LEVITATION PERm.NENT-m.GNET CERAm.CS CYLINDER SAm.LE

Vibrations in m.gnet/Superconductor Levitation System.

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Chinese Physics Letters 2006年第6期23卷 1641-1644页

作者： f. Y. Alzoubi H. m. Al-khateeb m. K. Alqadi N. Y. Ayoub Departm.nt of.Physics Jordan University of Science and Technology Irbid Jordan Departm.nt of.Physics Yarmouk University Irbid Jordan School of.Applied Natural Sciences German Jordanian University Amman Jordan

The problem.of.a sm.ll m.gnet levitating above a very thin superconducting disc in the m.issner state is analysed. The dipole-dipole interaction m.del is em.loyed to derive analytical expressions f.r the interaction energy, levitation f.rce, m.gnetic stif.ness and f.equency of.sm.ll vibrations about the equilibrium.position in two dif.erent conf.gurations, i.e. with the m.gnetic m.m.nt parallel and perpendicular to the superconductor. The results show that the f.equency of.sm.ll vibrations decreases with the increasing levitation height f.r a particular radius of.the superconducting disc, which is in good agreem.nt with the experim.ntal results. However, the f.equency increases m.notonically up to saturation by increasing the radius of.the disc f.r a particular height of.the m.gnet. In addition, the f.equency of.vibrations is higher when the system.is in the vertical conf.guration than that when the system.is in the horizontal conf.guration.

关键词： m.ISSNER STATE Sm.LL m.GNET f.RCE STIf.NESS SUPERCONDUCTORS SIZE

Ef.ects of.m.gnet Size and Geom.try on m.gnetic Levitation f.rce

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Chinese Physics Letters 2007年第9期24卷 2664-2666页

作者： m. K. Alqadi H. m. Al-khateeb f. Y. Alzoubi N. Y. Ayoub Departm.nt of.Physics Jordan University of Science and Technology Irbid Jordan School of.Natural Applied Sciences German Jordanian University Amman Jordan

We obtain analytical relations f.r the levitation f.rce as a f.nction of.dim.nsions of.the superconductor-m.gnet system. The f.rce has been calculated on the basis of.the dipole-dipole interaction m.del. The ef.ect of.thickness of.the superconductor on the levitation f.rce is investigated. The results show that the inf.uence of.geom.try and thickness of.the m.gnet becom.s signif.cantly large at sm.ll levitation distances. f.rtherm.re, approxim.ting the perm.nent m.gnet as a point dipole results in an inaccurate estim.tion of.the levitation f.rce.

关键词： m.ISSNER STATE