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隐形微球表面吸附血浆蛋白的蛋白质组学分析

中国科学：生命科学 2012年第3期42卷 185-195页

作者： Yevgeniya Le 李丽王敦成 Mark d.scott Canadian Blood Services VancouverBCV6T 1Z3Canada Centre for Blood Research University of British ColumbiaVancouverBCV6T 1Z3Canada department of Pathology and Laboratory Medicine University of British ColumbiaVancouverBCV6T 1Z3 Canada

细胞和生物材料表面的甲氧基聚乙二醇(mPEG)修饰是一项具有广阔应用前景的非药理性免疫调节技术,但由于细胞的不稳定性,目前对其表面与血浆间相互作用的认识还不够深入.本实验采用相对稳定的乳胶微球作为模型,来研究其表面对血浆蛋白的... 详细信息

细胞和生物材料表面的甲氧基聚乙二醇(mPEG)修饰是一项具有广阔应用前景的非药理性免疫调节技术,但由于细胞的不稳定性,目前对其表面与血浆间相互作用的认识还不够深入.本实验采用相对稳定的乳胶微球作为模型,来研究其表面对血浆蛋白的吸附.结果显示,血浆总蛋白的吸附水平随着mPEG修饰浓度和分子量的增加而降低,未修饰的乳胶微球表面蛋白吸附量为(159.9±6.4)ng/cm2,mPEG修饰后,蛋白吸附量分别下降至(18.4±0.8)ng/cm2(1mmol/L2kdSCmPEG)和(52.3±5.3)ng/cm2(1mmol/L20kdSCmPEG).聚炳烯酰胺凝胶电泳(SdS-PAGE)和同位素标记相对和绝对定量的质谱技术(iTRAQ-MS)分析表明,在PEG修饰微球表面的蛋白质吸附层中,参与免疫系统活化的蛋白质丰度明显下降.因此,免疫修饰细胞和材料的生物学效应是通过降低抗原性及减弱表面与大分子间相互作用来实现的.

关键词：聚乙二醇修饰免疫修饰同位素标记相对和绝对定量技术质谱分析聚苯乙烯乳胶微球蛋白质组学

Microsoft Office 2000开发指南

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Infection Prevalence of <i>Borrelia burgdorferi</i>in Ticks Collected from Songbirds in Far-Western Canada

丛书名：万水计算机技术实用大全系列

2000年

作者：（美）d.F.scott 余江林等译

本书介绍了VBA的功能、Basic语言本身的优点、VB中的代表性术语，分析了Office 2000可以进行哪些重构、Office 2000中的种种对象等内容。

ISBN: (纸本)7980044851

本书介绍了VBA的功能、Basic语言本身的优点、VB中的代表性术语，分析了Office 2000可以进行哪些重构、Office 2000中的种种对象等内容。

关键词：基本知识软件包

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Open Journal of Animal Sciences 2015年第3期5卷 232-241页

作者： John d. scott Lance A. durden John F. Anderson Research division Lyme Ontario Fergus Ontario Canada department of Biology Georgia Southern University Statesboro GA USA department of Entomology and Center for Vector Ecology and Zoonotic diseases The Connecticut Agricultural Experiment Station New Haven CT USA

Worldwide, wild birds play a vital role in the dispersal of ticks that harbour tick-borne pathogens, including Borrelia burgdorferi, the Lyme disease bacterium. Using PCR testing, we found 124 (31%) of 405 ticks (4 species), which were collected from 21 species of birds in far-western Canada, to be infected with B. burgdorferi. Transstadial transmission of B. burgdorferi occurred from larva to nymph, plus nymph to adult, in the avian coastal tick, Ixodes auritulus, collected from songbirds in British Columbia (B.C). Collectively, all 3 motile life stages (larva, nymph, adult) of this tick had an infection prevalence of 31% for B. burgdorferi, which suggests vector competency. A Pacific Wren was highly infested with I. auritulus immatures, and 20 (44%) of 45 ticks (2 nymphs, 43 larvae) were infected with B. burgdorferi. This heavy infestation shows the high potential to initiate a new population of ticks and to disseminate Lyme spirochetes. Epidemiologically, B. burgdorferi-infected I. auritulus larvae collected from the Spotted Towhee, Swainson’s Thrush, Pacific Wren, and Fox Sparrow suggest that these avian hosts act as reservoirs for B. burgdorferi. In this study, the western blacklegged tick, Ixodes pacificus, and Ixodes spinipalpis played a limited role in the enzootic transmission cycle of B. burgdorferi along coastal B.C. We document the first record of I. spinipalpis on a bird in Alberta. Because songbirds widely disperse Lyme disease vector ticks, primary health providers and the general public must be vigilant that outdoors people may be bitten by B. burgdorferi-infected ticks throughout far-western Canada.

关键词： Ticks Ixodes auritulus Ixodes pacificus Ixodes spinipalpis Lyme disease Borrelia burgdorferi Songbird Bird Parasitism Infection Prevalence

Smurf1拮抗BMP-2对C2C12细胞的诱导成骨作用

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复旦学报（医学版） 2006年第5期33卷 657-661页

作者：费琴明陈统一张光健 Boden scott d Titu Lsouisa 复旦大学附属中山医院骨科上海200032 爱默里大学医学院骨科和脊柱外科中心

目的研究Smad泛素化调节因子1(Smurf1)对基因重组人骨形态发生蛋白2(rhBMP-2)诱导成骨作用的影响。方法通过将质粒pcdEF3-Smurf1临时转染C2C12细胞并应用实时PCR和Western免疫印迹法鉴定其表达Smurf1,空载体pcdNA3.1作为阴性对照;然后... 详细信息

目的研究Smad泛素化调节因子1(Smurf1)对基因重组人骨形态发生蛋白2(rhBMP-2)诱导成骨作用的影响。方法通过将质粒pcdEF3-Smurf1临时转染C2C12细胞并应用实时PCR和Western免疫印迹法鉴定其表达Smurf1,空载体pcdNA3.1作为阴性对照;然后用比色法测定细胞碱性磷酸酶活性和实时PCR分析细胞碱性磷酸酶、骨钙素、成骨转录因子Osterix基因表达,观察Smurf1对rhBMP-2诱导C2C12细胞向成骨细胞分化的影响。同时在相差显微镜下观察细胞形态的变化。结果Smurf1在C2C12细胞可以通过临时转染获得很好的表达。尽管在细胞形态上没有明显的差别,但Smurf1转染的C2C12细胞在rhBMP-2作用48 h后,细胞碱性磷酸酶活性以及细胞碱性磷酸酶、骨钙素、成骨转录因子Osterix基因表达均较对照组明显下降。结论Smurf1在BMP诱导成骨过程中发挥着负性调节作用。

关键词：骨形态发生蛋白2 Smad泛素化调节因子1 转染 C2C12细胞

Bravo (wireless) ambulatory esophageal pH monitoring: How do day 1 and day 2 results compare?

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World Journal of Gastroenterology 2007年第30期13卷 4091-4095页

作者： Matthew L Bechtold Jason-scott L Holly Klaus Thaler John B Marshall division of Gastroenterology University of Missouri Hospital and Clinics Columbia MO 65212 United States General Surgery University of Missouri Hospital and Clinics Columbia MO 65212 United States

AIM： To investigate if differences exist for patients＇ gastroesophageal reflux as measured by the Bravo ambulatory esophageal pH system between d 1 and d 2.METHOdS： A retrospective study of 27 consecutive adult patients who underwent Bravo esophageal pH monitoring was performed. Patients underwent EGd under Ⅳ conscious sedation prior to Bravo placement. Acid reflux variables and symptom scores for d 1 were compared to d 2.RESULTS： The mean doses of fentanyl and midazolam were 90.4 μg and 7.2 mg, respectively. d 1 results were significantly more elevated than d 2 with respect to total time pH 〈 4, upright position reflux, and mean number of long refluxes. No statistical difference was noted between the two days for supine position reflux, number of refluxes, duration of longest reflux, episodes of heartburn, and symptom score.CONCLUSION： Patients undergoing Bravo esophageal pH monitoring in association with EGd and moderate conscious sedation experience significantly more acid reflux on d i compared to d 2. The Ⅳ sedation may be responsible for the increased reflux on d 1. Performed this way, 48-h Bravo results may not be entirely representative of the patients＇ true GE reflux profile.

关键词： Esophageal pH monitoring Bravo Gastroeso- phageal reflux disease Reflux pH testing

Associations of accelerometer-determined physical activity and sedentary behavior with sarcopenia and incident falls over 12 months in community-dwelling Swedish older adults

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Journal of Sport and Health Science 2021年第5期10卷 577-584页

作者： david scott Jonas Johansson Anoohya Gandham Peter R.Ebeling Peter Nordstrom Anna Nordstrom department of Medicine School of Clinical Sciences at Monash HealthMonash UniversityClaytonVIC 3168Australia department of Medicine and Australian Institute of Musculoskeletal Science Melbourne Medical School Western Campusthe University of MelbourneSt AlbansVIC 3021Australia department of Public Health and Clinical Medicine Section of Sustainable HealthUmea UniversityUmea SE-90187Sweden department of Community Medicine UiT the Arctic University of NorwayTromsø9010Norway department of Community Medicine and Rehabilitation Geriatric MedicineUmea UniversityUmea SE-90187Sweden School of Sport Sciences UiT the Arctic University of NorwayTromsø9010Norway

Purpose:This study was aimed to determine associations of accelerometer-determined time and bouts of sedentary behavior,light physical activity(LPA),and moderate-to-vigorous PA(MVPA)with sarcopenia and incident falls over 12 ***:A total of 3334 Swedish 70-year-olds were assessed for sarcopenia,as defined by the revised definition of the European Working Group on Sarcopenia in Older *** were based on low scores for appendicular lean mass(dual-energy X-ray absorptiometry),hand grip strength,and the Timed Up and Go *** 7 days after baseline,total time and total number of bouts(≥10 min of continuous activity at a given intensity)of activity performed at sedentary,LPA,and MVPA intensities were assessed by *** falls were self-reported 6 months and 12 months after ***:Only 1.8%of participants had probable or confirmed *** multivariable adjustment for other levels of activity,only greater MVPA time was associated with a decreased likelihood of having low appendicular lean mass,low hand grip strength,and slow Timed Up and Go time as defined by the European Working Group on Sarcopenia in Older People criteria(all pd only MVPA time was associated with lower likelihood of probable or confirmed sarcopenia(odds ratio=0.80,95%confidence interval:0.71-0.91 h/week).Similar associations were identified for total number of bouts,with no evidence of threshold effects for longer duration of bouts of MVPA.A total of 14%of participants reported-1 fall,but neither total time nor bouts of activity was associated with incident falls(all p>0.05).Conclusion:Higher amounts of accelerometer-determined MVPA are consistently associated with a decreased likelihood of sarcopenia and its components,regardless of the length of bouts or amounts of sedentary behavior.

关键词： Accelerometer Falls Older adults Physical activity Sarcopenia

Enduring alterations in hippocampal astrocytesynaptic proximity following adolescent alcohol exposure: reversal by gabapentin

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Neural Regeneration Research 2020年第8期15卷 1496-1501页

作者： Kati L.Healey Sandra Kibble Sierra Hodges Kathryn J.Reissner Anze Testen Tiffany A.Wills Shawn K.Acheson Benjamin M.Siemsen John A.McFaddin Michael d.Scofield H.scott Swartzwelder department of Psychiatry and Behavioral Sciences Duke University Medical CenterDurhamNCUSA department of Psychology and Neuroscience University of North CarolinaChapel HillNCUSA department of Cell Biology and Anatomy LSU School of MedicineNew OrleansLAUSA department of Anesthesia and Perioperative Medicine Medical University of South CarolinaCharlestonSCUSA

Adolescent alcohol abuse is a substantive public health problem that has been the subject of intensive study in recent *** reports of a wide range of effects of adolescent intermittent ethanol(AIE)exposure on brain and behavior,little is known about the mechanisms that may underlie those effects,and even less about treatments that might reverse *** studies from our laboratory have indicated that AIE produced enduring changes in astrocyte function and synaptic activity in the hippocampal formation,suggesting the possibility of an alteration in astrocyte-neuronal connectivity and *** utilized astrocyte-specific,membrane restricted viral labeling paired with immunohistochemistry to perform confocal single cell astrocyte imaging,three-dimensional reconstruction,and quantification of astrocyte morphology in hippocampal area CA1 from adult rats after ***,we assessed the colocalization of astrocyte plasma membrane labeling with immunoreactivity for AMPA-(α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)glutamate receptor 1,an AMPA receptor subunit and established neuronal marker of excitatory synapses,as a metric of astrocyte-synapse *** significantly reduced the colocalization of the astrocyte plasma membrane with synaptic marker puncta in *** is striking in that it suggests not only an alteration of the physical association of astrocytes with synapses by AIE,but one that lasts into adulthood-well after the termination of alcohol *** even more notable,the AIE-induced reduction of astrocyte-synapse interaction was reversed by sub-chronic treatment with the clinically used agent,gabapentin(Neurontin),in *** suggests that a medication in common clinical use may have the potential to reverse some of the enduring effects of adolescent alcohol exposure on brain *** animal experiments conducted were approved by the duke University Institutional Animal Care and Use Committee(Protocol

关键词： adolescent alcohol astrocyte astrocyte morphology astrocyte-neuronal colocalization CA1 gabapentin GluA1 hippocampus tripartite synapse

4号染色体短臂微卫星多态性与鼻咽癌相关性的研究

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遗传 2006年第7期28卷 783-790页

作者：郭秀婵 O’BRIEN stephen J WINKLER Cheryl scott Kevin HUTCHESON Holli dAVId Victor KESSING Bailey 郑裕明廖建刘彦 GUY de The 曾毅中国疾病预防控制中心病毒病预防控制所北京100052 美国国家癌症研究所基因组多样性研究室 frederickmd21702 广西梧州市肿瘤防治研究所梧州543002 广西苍梧县鼻咽癌防治所苍梧543100 法国巴士德研究所 parisfrance75724

鼻咽癌（NPC）是一种多因素复杂疾病。其发病过程涉及EB病毒慢性感染、环境致癌因素及宿主基因之间的相互作用。在这一过程中，那些宿主基因在EB病毒感染及鼻咽癌的发生发展中起了关键作用仍不清楚。本研究的目的是发现与鼻咽癌发生发... 详细信息

鼻咽癌（NPC）是一种多因素复杂疾病。其发病过程涉及EB病毒慢性感染、环境致癌因素及宿主基因之间的相互作用。在这一过程中，那些宿主基因在EB病毒感染及鼻咽癌的发生发展中起了关键作用仍不清楚。本研究的目的是发现与鼻咽癌发生发展中两个关键步骤相关的遗传变异，即EB病毒持续性感染鼻咽部上皮细胞和鼻咽癌的形成。我们在广西梧州市及苍梧县鼻咽癌高发区收集汉族鼻咽癌患者350例、EB病毒壳抗原IgA抗体阳性者（IgA／VCA＋）288例和EB病毒壳抗原IgA抗体阴性者（IgA／VCA—）346例。对先前鼻咽癌家系研究显示的鼻咽癌易感区4号染色体短臂（4p15．1～q12）进行了微卫星精细扫描，在18Mb的范围内选择34个微卫星标记，包括319个等位基因，对其进行基因分型。比较分析NPC组和IgA／VCA＋组等位基因频率结果显示，9个等位基因与鼻咽癌呈相关，其中5个为易感等位基因（OR=1．51～5．36，P=0．01～0．03），4个为限制性等位基因（OR值为0．3～0．71，P值为0．02～0．045）。比较分析IgA／VCA＋组和IgA／VCA-组及比较所有IgA／VCA＋者（包括NPC患者）和IgA／VCA-者等位基因频率的结果显示，12个等位基因与EB病毒壳抗原IgA抗体持续存在相关，其中3个在两组比较中均呈显著相关。等位基因d4S3241～136（P=0．004，OR=1．9，95％CI=1．2～3．0）和d4S3347-213（P=0．001，OR=1．6，95％CI=1．2～2．1）可增加EB病毒IgA／VCA抗体形成的危险，为易感基因；而等位基因d4S174．202（P=0．001，OR=0．5，95％CI=0．3～0．7）可限制IgA／VCA抗体的形成。但上述结果经多因素比较校正后，均失去相关性。结果不能确定该区域与非家族性鼻咽癌的形成相关，但本研究却提供了进一步发现鼻咽癌相关基因的研究模式。有关4号染色体短臂与EB病毒慢性持续感染及鼻咽癌的形成仍值得进一步深入研究。

关键词：鼻咽癌 4号染色体微卫星相关研究

Top quark pair production at complete NLO accuracy with NNLO+NNLL′corrections in QCd

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Chinese Physics C 2020年第8期44卷 40-45页

作者： MichałCzakon Andrea Ferroglia Alexander Mitov davide Pagani Andrew S.Papanastasiou Benjamin d.Pecjak darren J.scott Ioannis Tsinikos Xing Wang Li Lin Yang Marco Zaro Institut für Theoretische Teilchenphysik und Kosmologie RWTH Aachen UniversityD-52056 AachenGermany New York City College of Technology BrooklynNY 11201USA The Graduate School and University Center The City University of New YorkNew YorkNY 10016 USA Cavendish Laboratory University of CambridgeCB30HE CambridgeUK Technische Universität München James-Franck-Str.1D-85748 GarchingGermany Theory Group Deutsches Elektronen-Synchrotron(DESY)D-22607 HamburgGermany Institute for Particle Physics Phenomenology University of DurhamDH13LE DurhamUK Institute for Theoretical Physics University of AmsterdamScience Park 9041098 XH AmsterdamThe Netherlands Nikhef Theory GroupScience Park 1051098 XGAmsterdamThe Netherlands Theoretical Particle Physics Department of Astronomy and Theoretical PhysicsLund UniversitySölvegatan 14ASE-22362 LundSweden PRISMA+Cluster of Excellence Johannes Gutenberg UniversityD-55128 MainzGermany Zhejiang Institute of Modern Physics Department of PhysicsZhejiang UniversityHangzhou 310027China INFN Sezione di MilanoVia Celoria 1620133 MilanoItaly

We describe predictions for top quark pair differential distributions at hadron colliders,by combining the next-to-next-to-leading order quantum chromodynamics calculations and next-to-leading order electroweak corrections with double resummation at the next-to-next-to-leading logarithmic accuracy of threshold logarithms and small-mass *** the best of our knowledge,this is the first study to present such a combination,which incorporates all known perturbative *** results are presented for the invariant-mass distribution,transverse-momentum distribution,and rapidity distributions.

关键词： top quark production QCd electroweak