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世界最新医学信息文摘 2017年第30期 281-281页

作者： Qiuwei Pan scot d.henry Herold J.Metselaar Bob Scholte Jaap Kwekkeboom Hugo W.Tilanus Harry L.A.Janssen Luc J.W.van der Laan

The current standard interferon-alpha(IFN-α)-based therapy for chronic hepatitis C virus(HCV) infection is only effective in approximately half of the patients,prompting the need for alternative *** interference(RNAi)represents novel approach to combat HCV by sequence-specific targeting of viral or host factors involved in *** of RNAi,however,may lead to therapeutic resistance by mutational escape of the ***,we proposed that combining lentiviral vector-mediated RNAi and IFN-a could be more effective and avoid therapeutic *** this study,we found that IFN-α treatment did not interfere with RNAi-mediated gene *** and IFN-α act independently on HCV replication showing combined antiviral activity when used simultaneously or *** ofmouse hepatocytes in vivo and in vitro was not effected by IFN-α *** conclusion,RNAi and IFN-a can be effectively combined without crossinterference and may represent a promising combinational strategy for the treatment of hepatitis C.

关键词： RNAi IFN-α Gene therapy Lentiviral vector HCV

Combined antiviral activity of interferon-α and RNA interference directed against hepatitis C without affecting vector delivery and gene silencing

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世界最新医学信息文摘 2016年第11期 8-8页

作者： Qiuwei Pan scot d.henry Herold J.Metselaar Bob Scholte Jaap Kwekkeboom Hugo W.Tilanus Harry L.A.Janssen Auc J.W.van der Laan

The current standard interferon-alpha(IFN-α)-based therapy for chronic hepatitis C virus(HCV) infection is only effective in approximately half of the patients, prompting the need for alternative treatments. RNA interference(RNAi) represents novel approach to combat HCV by sequence-specific targeting of viral or host factors involved in infection. Monotherapy of RNAi, however, may lead to therapeutic resistance by mutational escape of the virus. Here, we proposed that combining lentiviral vector-mediated RNAi and IFN-α could be more effective and avoid therapeutic resistance. In this study, we found that IFN-α treatment did not interfere with RNAi-mediated gene silencing. RNAi and IFN-α act independently on HCV replication showing combined antiviral activity when used simultaneously or sequentially. Transduction ofmouse hepatocytes in vivo and in vitro was not effected by IFN-α treatment. In conclusion, RNAi and IFN-α can be effectively combined without crossinterference and may represent a promising combinational strategy for the treatment of hepatitis c.

关键词： RNAi IFN-α Gene therapy Lentiviral vector HCV

Transient growth in Poiseuille-Rayleigh-Bénard flows of binary fluids with Soret effect

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Applied Mathematics and Mechanics(English Edition) 2016年第9期37卷 1203-1218页

作者： Jun HU d.henry H.BENHAdId Xieyuan YIN Institute of Applied Physics and Computational Mathematics Beijing 100088China Laboratoire de Mecanique des Fluides et d'Acoustique CNRS/Universitd de LyonEcole Centrale de Lyon/universite Lyon 1/INSA de LyonECL36 avenue Guy de Collongue69134 Ecully CedexFrance department of Modern Mechanics University of Science and Technology of ChinaHefei 230027China

The transient growth due to non-normMity is investigated for the Poiseuille- Rayleigh-Benard problem of binary fluids with the Soret effect. For negative separation factors such as ψ = -0.1, it is found that a large transient growth can be obtained by the non-normal interaction of the two least-stable-modes, i.e., the upstream and downstream modes, which determine the linear critical boundary curves for small Reynolds numbers. The transient growth is so strong that the optimal energy amplification factor G（t） is up to 10^2 - 10^3. While for positive separation factors such as ψ = 0.1, the transient growth is weak with the order O（I） of the amplification factor, which can even be computed by the least-stable-mode. However, for both cases, the least-stable-mode can govern the long-term behavior of the amplification factor for large time. The results also show that large Reynolds numbers have stabilization effects for the maximum amplification within moderate wave number regions. Meanwhile, much small negative or large positive separation factors and large Rayleigh numbers can enlarge the maximum transient growth of the pure streamwise disturbance with the wavenumber α= 3.14. Moreover, the initial and evolutionary two-dimensional spatial patterns of the large transient growth for the pure streamwise disturbance are exhibited with a plot of the velocity vector, spanwise vorticity, temperature, and concentration field. The initial three-layer cell vorticity struc- ture is revealed. When the amplification factor reaches the maximum Gmax, it develops into one cell structure with large amplification for the vorticity strength.

关键词： binary fluid Soret effect transient growth Poiseuille-Rayleigh-Benard flow

Experimental Investigation of the Natural and Forced Convection on Solidification of Sn-3wt.%Pb Alloy Using a Benchmark Experiment

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Journal of Iron and Steel Research International 2012年第S1期19卷 286-290页

作者： L.Hachani R.Boussaa B.Saadi X-d.Wang K.Zaidat A.Belgacem Bouzida d.henry V.Botton H.Ben Hadid Y.Fautrelle Grenoble Institute of Technology SIMAP-EPM LaboratoryPHELMA BP75 38402 Saint Martin d'Hères CedexFrance Laboratory of Mechanics University Amar Telidji of LaghouatAlgeria University Hadj Lakhdar of Batna Algeria LMFA Ecole Centrale de Lyon 36 avenue Guy de Collongue69134 Ecully CedexFrance GUCAS

We deal with the development of a solidification benchmark experiment in order to investigate the structure formation as well as solute macro-mesosegregation,by means of a well-controlled solidification *** experiment consists in solidifying a rectangular ingot of Sn-3wt.%Pb alloy,by using two lateral heat exchangers which allow extraction of the heat flux from one or two vertical sides of the *** domain is a quasi two dimensional parallepipedic ingot(100×60×10)*** temperature difference AT between the two lateral sides is 40 K and the cooling rate CR=0.03 K/*** instrumentation consists in recording the instantaneous temperature maps by means of an array of 50 thermocouples in order to provide the time evolution of the *** each experiment the patterns of the segregations have been obtained by X-ray radiograph and confirmed by eutectic fraction *** local solute distribution determined by means of induction coupled plasma analysis is *** originality of the present study is to examine the effect of the forced convection driven by a travelling magnetic field(TMF)induced by a linear inductor located on the bottom part of the sample.A periodically reversed stirring with a modulation frequency equal to 0.5 Hz stirring have been *** study allows us to evaluate the evolution due to the forced convection induced by a TMF field,as well as its influence on the initial conditions,the solidification macrostructure and the segregation *** of the velocity field by ultrasonic doppler velocimetry(UdV)method in a Ga-In-Sn pool were performed and transposed to the tin-lead alloy case before ***-mortem patterns of the macromesosegregations have been obtained by X-ray *** results show the transport effects of the flow on both the maerosegregations and the channel *** reversal of the TMF produces a decrease of the level of mesosegregations,namely channel formation

关键词： In Sn Pb Alloy Using a Benchmark Experiment Experimental Investigation of the Natural and Forced Convection on Solidification of Sn-3wt

Using machine learning and a data-driven approach to identify the small fatigue crack driving force in polycrystalline materials

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npj Computational Materials 2018年第1期4卷 363-372页

作者： Andrea Rovinelli Michael d.Sangid.henry Proudhon Wolfgang Ludwig School of Aeronautics and Astronautics Purdue University701W.Stadium AveWest LafayetteIN 47907USA MINES ParisTech PSL Research UniversityMAT-Centre des matériauxCNRS UMR 7633BP 8791003 EvryFrance University of Lyon INSA LyonMATEISUMR 5510 CNRSF-69621 LYONFrance

The propagation of small cracks contributes to the majority of the fatigue lifetime for structural *** significant interest,criteria for the growth of small cracks,in terms of the direction and speed of crack advancement,have not yet been *** this work,a new approach to identify the microstructurally small fatigue crack driving force is *** network and machine learning techniques are utilized to identify relevant micromechanical and microstructural variables that influence the direction and rate of the fatigue crack propagation.A multimodal dataset,combining results from a high-resolution 4d experiment of a small crack propagating in situ within a polycrystalline aggregate and crystal plasticity simulations,is used to provide training *** relevant variables form the basis for analytical expressions thus representing the small crack driving force in terms of a direction and a rate *** ability of the proposed expressions to capture the observed experimental behavior is quantified and compared to the results directly from the Bayesian network and from fatigue metrics that are common in the *** indicate that the direction of small crack propagation can be reliably predicted using the proposed analytical model and compares more favorably than other fatigue metrics.

关键词： fatigue crack driving

Influences of load carriage and physical activity history on tibia bone strain

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Journal of Sport and Health Science 2019年第5期8卷 478-485页

作者： henry Wang Mohammad Kia d.Clark dickin Biomechanics Laboratory School of KinesiologyBall State UniversityMuncieIN 47306USA department of Biomechanics Hospital for Special SurgeryNew YorkNY 10021USA

Background:Military recruits are often afflicted with stress *** military’s strenuous training programs involving load carriage may contribute to the high incidence of tibia stress fractures in the *** purpose of this study was to assess the influences of incremented load carriage and history of physical activity on tibia bone strain and strain rate during ***:Twenty recreational basketball players and 20 recreational runners performed 4 walking tasks while carrying 0 kg,15 kg,25 kg,and35 kg loads,*** bone strain and strain rate were obtained through subject-specific multibody dynamic simulations and finite element *** model repeated-measures analyses of variance were ***:The mean±SE of the runners’ bone strain(μs)during load carriages(0 kg,15 kg,25 kg,and 35 kg)were 658.11±1.61,804.41±1.96,924.49±2.23,and 1011.15±2.71,respectively,in compression and 458.33±1.45,562.11±1.81,669.82±2.05,and 733.40±2.52,respectively,in *** the basketball players,the incremented load carriages resulted in compressive strain of 634.30±1.56,746.87±1.90,842.18±2.16,and 958.24±2.63,respectively,and tensile strain of 440.04±1.41,518.86±1.75,597.63±1.99,and 700.15±2.47,respectively.A dose-response relationship exists between incremented load carriage and bone strain and strain rate.A history of regular basketball activity could result in reduced bone strain and reduced strain ***:Load carriage is a risk factor for tibia stress fracture during basic *** exercise programs,such as basketball,that involved multidirectional mechanical loading to the tibia bones can be implemented for military recruits before basic training commences.

关键词： Modeling Multi-body dynamics Strain Strain rate Stress fracture Walking

New therapeutic opportunities for Hepatitis C based on small RNA

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World Journal of Gastroenterology 2007年第33期13卷 4431-4436页

作者： Qiu-wei Pan scot d.henry Bob J Scholte Hugo W Tilanus Harry LA Janssen Luc JW van der Laan departments of Gastroenterology & Hepatology and Surgery Erasmus MC-University Medical Centre Rotterdam The Netherlands department of Surgery Erasmus MC-University Medical Centre Rotterdam The Netherlands department of Cell Biology Erasmus MC- University Medical Centre Rotterdam The Netherlands department of Gastroenterology & Hepatology Erasmus MC-University Medical Centre Rotterdam The Netherlands

Hepatitis C virus （HCV） infection is one of the major causes of chronic liver disease, including cirrhosis and liver cancer and is therefore, the most common indication for liver transplantation. Conventional antiviral drugs such as pegylated interferon-alpha, taken in combination with ribavirin, represent a milestone in the therapy of this disease. However, due to different viral and host factors, clinical success can be achieved only in approximately half of patients, making urgent the requirement of exploiting alternative approaches for HCV therapy. Fortunately, recent advances in the understanding of HCV viral replication and host cell interactions have opened new possibilities for therapeutic intervention. The most recent technologies, such as small interference RNA mediated gene-silencing, antisense oligonucleotides （ASO）, or viral vector based gene delivery systems, have paved the way to develop novel therapeutic modalities for HCV. In this review, we outline the application of these technologies in the context of HCV therapy. In particular, we will focus on the newly defined role of cellular microRNA （miR-222） in viral replication and discuss its potential for HCV molecular therapy.

关键词： Hepatitis C virus therapy miR-122 RNAi Antisense oligonucleotides Viral vectors

Energy expenditure and muscular activation patterns through active sitting on compliant surfaces

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Journal of Sport and Health Science 2017年第2期6卷 207-212页

作者： d.Clark dickin Rachel K.Surowiec henry Wang Biomechanics Laboratory Ball State UniversityMuncieIN 47306USA

Purpose: To test the effectiveness of sitting surfaces with varied amounts of stability on muscle activity and energy ***: Using a within-participants repeated measures design, 11 healthy young-adult females(age = 20.0 ± 1.8 years) were measured using indirect calorimetry to assess energy expenditure, and electromyography to assess muscular activation in trunk and leg musculature under 3different sitting surfaces: flat-firm surface, air-filled cushion, and a stability ball. data were analyzed using repeated measures analysis of variance with follow-up pairwise contrasts used to determine the specific effects of sitting surface on muscle activation and energy ***: Significantly greater energy expenditure was recorded for the stability ball(p = 0.01) and the cushion(p = 0.03) over the flat surface(10.4% and 9.6% greater, respectively), with no differences between the ball and the cushion. Both the ball and the cushion produced higher tibialis anterior activation over the flat surface(1.09 and 0.63 root-mean-square millivolts(RMSmv), respectively), while the stability ball produced higher soleus activity over both cushion and flat surfaces(3.97 and 4.24 RMSmv, respectively). Additionally, the cushion elicited higher adductor longus activity over the ball and flat surfaces(1.76 and 1.81 RMSmv, respectively), but no trunk musculature differences were ***: Compliant surfaces resulted in higher levels of muscular activation in the lower extremities facilitating increased caloric *** the increasing trends in sedentary careers and the increases in obesity, this is an important finding to validate the merits of active sitting facilitating increased caloric expenditure and muscle activation.

关键词： Energy metabolism Electromyography Female Posture Sedentary lifestyle Workplace

Tissue engineering approaches for dental pulp regeneration:The development of novel bioactive materials using pharmacological epigenetic inhibitors

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Bioactive Materials 2024年第10期40卷 182-211页

作者： Ross M.Quigley Michaela Kearney Oran d.Kennedy henry F.duncan division of Restorative dentistry&Periodontology Dublin Dental University HospitalTrinity College Dublin(TCD)University of DublinLincoln PlaceDublinIreland department of Anatomy and Regenerative Medicine and Tissue Engineering Research GroupRoyal College of Surgeons in Ireland(RCSI)University of Medicine and Health SciencesDublinIreland The Trinity Centre for Biomedical Engineering(TCBE)and the Advanced Materials and Bioengineering Research Centre(AMBER) Royal College of Surgeons in Ireland(RCSI)and Trinity College Dublin(TCD)DublinIreland

The drive for minimally invasive endodontic treatment strategies has shifted focus from technically complex and destructive root canal treatments towards more conservative vital pulp ***,novel approaches to maintaining dental pulp vitality after disease or trauma will require the development of innovative,biologicallydriven regenerative medicine *** example,cell-homing and cell-based therapies have recently been developed in vitro and trialled in preclinical models to study dental pulp *** approaches utilise natural and synthetic scaffolds that can deliver a range of bioactive pharmacological epigenetic modulators(HdACis,dNMTis,and ncRNAs),which are cost-effective and easily applied to stimulate pulp tissue ***,many biological factors hinder the clinical development of regenerative therapies,including a lack of blood supply and poor infection control in the necrotic root canal *** challenges include a need for clinically relevant models and manufacturing challenges such as scalability,cost concerns,and regulatory *** review will describe the current state of bioactive-biomaterial/scaffold-based engineering strategies to stimulate dentine-pulp regeneration,explicitly focusing on epigenetic modulators and therapeutic pharmacological *** will highlight the components of dental pulp regenerative approaches,describe their current limitations,and offer suggestions for the effective translation of novel epigenetic-laden bioactive materials for innovative therapeutics.

关键词： Epigenetics Regenerative medicine Regenerative endodontics Biocompatible materials Tissue engineering

Implementation of length expanding inflatable penile prosthesis is not sufficient to prevent postsurgical penile shortening

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Asian Journal of Andrology 2019年第1期21卷 98-100页

作者： Jared J Wallen SriGita K Madiraju Run Wang Gerard d.henry department of Urology Florida Hospital Tampa UrologyTampaFL 3100USA Charles E Schmidt College of Medicine of FAU Boca RatonFL 33431USA department of Urology University of Texas Health Science Center at HoustonHoustonTX 77030USA department of Urology Ark-La-Tex UrologyBossier CityLA 71111USA.

Asingle armed,prospective,two-center study was conducted,with an aim of evaluating the effectiveness of the AMS 700 LGX cylinders in maintaining or increasing penile length *** LGX has the unique ability to expand in length with *** 23.1%(6)of the patients were able to maintain stretched penile *** now recommend leaving the implant inflated 60%-80%in the immediate postoperative setting for approximately 6 *** we teach pump training with deflation at around 6 weeks,prior to a maximum inflation *** studies would be required to validate if these interventions have significantly increased the number of patients who maintain or gain stretched penile length.

关键词： penile prosthesis penile shortening