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Influence of altitude environment on the adult neurogenesis in mice

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Influence of altitude environment on the adult neurogenesis ...

疏水层析用于大规模纯化重组HBsAg的工艺研究

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Bing-Feng LIU~1,Wen-Qiang XU~1,Xue ZHENG~1,Ping-Jing GUO~1,Li FAN~1,Jia-Wei LI~1,Jian-Lin WANG~1, Shan-Ting ZHAO~(1,2,*) 1 Institute of Zoology,school of life science,lanzhou university,lanzhou 730000,china 2 Institute of Anatomy and Cell Biology,university of Freiburg,Freiburg,79104,Germany

＜正＞Introduction In recent years,people have found that neural stem cells still exist in human and animals after the development and these neural stem cells can constantly generate new neurons and astrozytes in life *** most adult mammalian CNS,active neurogenesis occurs throughout life in the subventricular zone（SVZ） of the lateral ventricle and in the subgranular zone（SGZ） of the dentate gyrus in the ***,the mechanisms of proliferation,differentiation and survival about adult neurogenesis remain *** has been found that experimental changes of living environment can significantly affect the proliferation,differentiation and survival of neural stem cells in the SGZ of the dentate ***,it has not been reported that how the changes of natural environment affect the adult *** The aim of our study is to investigate the influence of high-altitude living environment in the Qinghai-Tibet Plateau on the proliferation and differentiation of adult neural stem cells in the SGZ of the dentate gyrus in mice,and we expect that the results of our experiment can provide evidences for elucidating the mechanism of adult *** Healthy adult Kunming mice were divided into 2 groups:one altitude of control group is 1 500 m in lanzhou,the other altitude of experimental group is 4 600 m in Tuotuohe on Qinghai-Tibet *** label the newly generated cells 5-bromine deoxidizing urine nucleoside（BrdU,50 mg/kg） were injected intraperitoneally for 6 times within 3 *** immunocytochemistry against BrdU and double immunocytochemistry against BrdU and Prox-1 were ***,the newly generated BrdU-postive cells and double labeled cells for BrdU and Prox-1 were counted in laser confocal microsopy photographs,*** The BrdU-positive cells of experimental group significantly reduced to 60%compared with the control group（P = 0.001）,indicating that the high-altitude（low oxygen） environment inhibit the pro

关键词： adult neural stem cell hypoxemiahypoxia dentate gyrus Prox-1 BrdU

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微生物学免疫学进展 2003年第3期31卷 29-31页

作者：姚伟李建民毛晓燕董继刚张秋峰兰州生物制品研究所兰州730046 兰州大学生命科学学院兰州730000

应用疏水层析法从CHO细胞培养液中纯化HBsAg,每根制备柱每次可处理细胞收液 35 0L ,在适宣的上样流速和层析温度条件下 ,层析后可去除 96 %的杂蛋白 ,再经超速离心和凝胶过滤层析 ,可获HBsAg纯品。经检定 ,HPLC纯度高于 95 % ,其余各项... 详细信息

应用疏水层析法从CHO细胞培养液中纯化HBsAg,每根制备柱每次可处理细胞收液 35 0L ,在适宣的上样流速和层析温度条件下 ,层析后可去除 96 %的杂蛋白 ,再经超速离心和凝胶过滤层析 ,可获HBsAg纯品。经检定 ,HPLC纯度高于 95 % ,其余各项检定指标均符合《中国生物制品规程》要求。结果表明 ,此方法纯化效率高、处理样品量大、成本低。

关键词：疏水作用层析纯化重组乙肝表面抗原

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一氧化氮对植物生长发育的作用

天水师范学院学报 2004年第5期24卷 23-25页

作者：王弋博曲颖程佳强王勋陵天水师范学院生命科学与化学学院甘肃天水741001 兰州大学生命科学学院甘肃兰州730000

一氧化氮(NO)是一种气体信号分子,参与调控植物的许多重要生理过程,介绍了NO信号分子的发现,内源NO的产生以及NO对植物生长发育的作用。

关键词：一氧化氮(NO) 气体信号分子作用生理过程调控发现参与植物生长发育

白阿魏菇菌丝体多糖(CPNMP)的药理作用

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西北民族大学学报（自然科学版） 2004年第1期25卷 73-79页

作者：李永泉吴炬花立明吴应文张育康李强杨五虎西北民族大学医学院甘肃兰州730030 兰州大学生命科学学院甘肃兰州730000 甘肃省草原总站甘肃兰州730000 平川一中生物教研室甘肃白银730914 平凉林业处甘肃平凉744000 白堡中学甘肃庄浪744600

用沸水提取白阿魏菇菌丝体多糖 ,经除蛋白 ,醇析得粗多糖 (CPNMP) ,含多糖 5 9.2 % 进行免疫、抗炎和抗肿瘤实验 ,结果 :CPNMP能增加中性红吞噬指数 (P <0 .0 5 ) ,促进DTH的发生 (P <0 .0 1) ,促进红细胞SOD(P <0 .0 1) ,血清SOD(P <0... 详细信息

用沸水提取白阿魏菇菌丝体多糖 ,经除蛋白 ,醇析得粗多糖 (CPNMP) ,含多糖 5 9.2 % 进行免疫、抗炎和抗肿瘤实验 ,结果 :CPNMP能增加中性红吞噬指数 (P <0 .0 5 ) ,促进DTH的发生 (P <0 .0 1) ,促进红细胞SOD(P <0 .0 1) ,血清SOD(P <0 .0 1)、CAT(P <0 .0 1)和溶菌酶活力 (P <0 .0 1) ,降低MDA(P <0 .0 5 ) ,而且对胆固醇有促进作用 (P<0 .0 5 ) ;CPNMP能明显抑制二甲苯所致小鼠耳肿胀 ,抑制小鼠毛细血管通透性 ;CPNMP还能明显地抑制S180 的瘤重 ,抑瘤率为 39.1% ,提高血清SOD(P <0 .0 5 )、CAT(P <0 .0 1)溶菌酶 (P <0 .0 1)和LDH -L(P <0 .0 5 )的活力 ;增加免疫器官的重量 (P <0 .0 1) ,降低MDA(P <0 .0 5 )和ALP活力 (P <0 .0 5 ) .因此CPNMP有提高免疫力 ,抗炎。

关键词：白阿魏菇菌丝体多糖免疫抗炎抗肿瘤药理作用

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白阿魏菇深层发酵工艺的研究

西北民族大学学报（自然科学版） 2003年第4期24卷 14-18页

作者：李永泉吴炬杨全珍秦卫平沈东华吴应文西北民族大学医学院甘肃兰州730030 兰州大学生命科学院甘肃兰州730000

用单因子实验、正交实验,检测白阿魏菇深层发酵指数,结果为:摇瓶发酵的最佳条件为温度25℃,装液量为三角瓶体积的1/5,培养基起始pH值5.5,接种量10%,大号玻璃珠12个/瓶通过单因子和正交实验可知,菌丝体生长的最佳碳源为土豆汁;发酵罐最... 详细信息

用单因子实验、正交实验,检测白阿魏菇深层发酵指数,结果为:摇瓶发酵的最佳条件为温度25℃,装液量为三角瓶体积的1/5,培养基起始pH值5.5,接种量10%,大号玻璃珠12个/瓶通过单因子和正交实验可知,菌丝体生长的最佳碳源为土豆汁;发酵罐最佳培养基配方为葡萄糖0.5%,蔗糖0.5%,玉米粉5%,麸皮4%,蛋白胨0.2%,KH2PO40.1%,MgSO4·7H2O0.05%,羧甲基纤维素钠0.5%,VB1微量;

关键词：白阿魏菇深层发酵单因子实验正交实验药用价值人工栽培

A computer simulation of nucleation and growth of thin films

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science china(Physics,Mechanics & Astronomy) 2004年第4期47卷 442-451页

作者： ZHENG Xiaoping1,ZHANG Peifeng2,HE Deyan2,LIU Jun2& MA Jiantai3 1. Key Laboratory of Opto-Electronic Technology and Intelligent Control, Ministry of Education, lanzhou Jiaotong university, lanzhou 730070, china 2. school of Physical science and Technology, lanzhou university, lanzhou 730000, china 3. school of Chemistry and Chemistry Engineering, lanzhou university, lanzhou 730000, china Key Laboratory of Opto-Electronic Technology and Intelligent Control Ministry of Education Lanzhou Jiaotong University Lanzhou China school of Physical science and Technology Lanzhou University Lanzhou China school of Chemistry and Chemistry Engineering Lanzhou University Lanzhou China

A three-dimensional kinetic Monte Carlo technique has been developed for simulating the nucleation and growth of thin films. This model involves incident atom attachment, surface diffusion of the atoms on the growing surface and atom detachment from the growing surface. It takes some new effects into account, such as a significant improvement in calculation of activation energy for the atom diffusion, which renders the model more reasonable. In addition three optimum temperatures and the consistency of their dependence on deposition rate have been found out; the dependence of the surface roughness and relative density on the deposition rate has been discussed; and the approximation of freezing neighbour atoms and periodic boundary conditions has been applied.

关键词： Monte Carlo, thin film, nucleation, surface roughness, relative density.

Eu(III) adsorption on rutile:Batch experiments and modeling

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science china Chemistry 2010年第12期53卷 2628-2636页

作者： WANG XueFeng,SHI KeLiang,GUO ZhiJun & WU WangSuo Radiochemistry Lab,school of Nuclear science and Technology,lanzhou university,lanzhou 730000,china Radiochemistry Lab School of Nuclear Science and Technology Lanzhou University Lanzhou China

Eu(III) adsorption on rutile was investigated as a function of contact time,pH,ionic strength and Eu(III) concentration by using a batch experimental *** effects of carbonate,sulfate,and phosphate were also *** was found that the kinetics of Eu(III) adsorption on rutile could be described by a pseudo-second-order *** adsorption of Eu(III) on rutile is strongly pH-dependent,but relatively insensitive to ionic strength.A double layer model (DLM) with two inner-sphere Eu(III) surface complexes was applied to quantitatively interpret the adsorption of Eu(III) on *** were no apparent effects of carbonate and sulfate on Eu(III) adsorption,whereas the presence of phosphate promoted Eu(III) adsorption on *** surface complexes of Eu(III) on rutile were evidenced by X-ray photoelectron spectroscopy (XPS).

关键词： Eu(III) adsorption rutile kinetics surface complexation modeling XPS

Vicissitude of Sogo Nur and environmental-climatic change during last 1500 years

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science china Earth sciences 2004年第Z1期47卷 61-70页

作者： JIN Heling, XIAO Honglang, SUN Liangying, ZHANG Hong, SUN Zhong & LI XiaozeKey Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of sciences, lanzhou 730000, china school of Resources and Environment, lanzhou university, lanzhou 730000, china Key Laboratory of Desert and Desertification Cold and Arid Regions Environmental and Engineering Research Institute Chinese Academy of Sciences Lanzhou China school of Resources and Environment Lanzhou University Lanzhou China

Lakes in arid zone are sensitive to climatic changes. The lacustrine sediment sequence in Sogo Nur has well and truly recorded climatic events such as the Sui-Tang Dynasty Warm Period, the Song-Liao Dynasty Cold Period, the Medieval Warm Period, the Little Ice Age and the 20th Century Warm Period. Commonly, the climate in warm Periods was relatively humid,accordingly the lake area extended and water level rose, and vice versa. Apart from climatic change, human activity is also an important factor of influencing lake vicissitude, and they played the dominant role alternatively during different periods; the factor of climatic change predominated in historical period, while since the beginning of the 20th century the utilization of water resources by human has became decisive.

关键词： granularity, soluble salt, lake vicissitude, climatic change, Sogo Nur.

On the Diction Characteristics of English Newspaper Advertisement

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兰州大学学报（社会科学版） 2000年第S1期28卷 232-241页

作者： Du Juan (school of Foreign Languages, lanzhou university, lanzhou, 730000, china)

Based on a systematic analysis of newspaper advertisements the paper intends to explore the language characteristics of English advertisements in the aspect of word choice and grammatical patterns. In this way, it wil... 详细信息

关键词： advertisement word choice grammatical patterns