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Interspecific delimitation and phylogenetic origin of Pugionium(Brassicaceae)

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Journal of Systematics and Evolution 2010年第3期48卷 195-206页

作者： 1,2,3Qiu-Shi YU 1Qian WANG 1Ai-Lan WANG 1Gui-Li WU 3Jian-Quan LIU 1(Division of Molecular Ecology,Key Laboratory of Arid and Grassland Ecology,school of life science,lanzhou university,lanzhou 730000,china) 2(Minqin National Studies Station for Desert Steppe Ecosystem,Minqin 733300,china) 3(Gansu Desert Control Research Institute,lanzhou 730070,china) (Division of Molecular Ecology Key Laboratory of Arid and Grassland Ecology School of Life Science Lanzhou University Lanzhou 730000 China) (Minqin National Studies Station for Desert Steppe Ecosystem Minqin 733300 China) (Gansu Desert Control Research Institute Lanzhou 730070 China)

Pugionium(Brassicaceae)is a small genus that occurs in central Asian *** interspecific delimitation and taxonomic treatments of this genus are disputed and its phylogenetic origin remains unknown. In the present study,we examined these issues based on morphological and molecular data obtained for the first *** used statistical methods to examine inter-and intraspecific morphological *** results suggest that only two species,namely *** and ***,can be warranted for all examined populations and specimens,whereas three species(***,***,and ***)should be incorporated into P. *** delimitation was further supported by the molecular data:all populations of ***,P. calcaratum,***,and *** shared the same internal transcribed spacer genotype,whereas those from *** had another *** analyses of Pugionium and representative genera of Brassicaceae based on ndhF sequences suggest that this genus is sister to the genus Megacarpaea,which,together,comprise a well-supported lineage with Farsetia,Lobularia,Iberis,and Ionopsidium,whereas the two other genera that were previously suggested to be closely related to this genus(Isatis and Bunias)were placed in the other *** further discuss the origin of Pugionium and suggest that it probably originated in central Asia when the climate became drier from the late Miocene.

关键词： internal transcribed spacer(ITS) interspecific delimitation morphological variation ndhF phyloge- netic origin Pugionium.

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孤啡肽受体的配体研究进展

生物化学与生物物理进展 2004年第2期31卷 106-111页

作者：陈勇常民王锐兰州大学生命科学学院生物化学与分子生物学系兰州730000

孤啡肽 (nociceptin或orphaninFQ)发现于 1 995年底 ,它是阿片受体样受体 (ORL1或LC 1 32 )的内源性配体 ,在痛觉调节、心血管系统、离子通道、依赖和耐受、学习和记忆等方面具有广泛的生物学活性 .最近几年 ,对孤啡肽受体与相关配体构... 详细信息

孤啡肽 (nociceptin或orphaninFQ)发现于 1 995年底 ,它是阿片受体样受体 (ORL1或LC 1 32 )的内源性配体 ,在痛觉调节、心血管系统、离子通道、依赖和耐受、学习和记忆等方面具有广泛的生物学活性 .最近几年 ,对孤啡肽受体与相关配体构效关系的研究成为一个新的热点 .对在研究构效关系过程中所发现的孤啡肽受体相关配体 (片段、拮抗剂、激动剂、部分激动剂和阻断剂 )的研究情况进行了介绍 .

关键词：孤啡肽 0RLl受体配体片段激动剂部分激动剂拮抗剂

Demographic history of the Tibetan antelope Pantholops hodgsoni(chiru)

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Journal of Systematics and Evolution 2010年第6期48卷 490-496页

作者： 1,2,3Yu-Rong DU 1Song-Chang GUO 4Zhao-Feng WANG 1Hai-Xing CI 1Zhen-Yuan CAI 4Qian ZHANG 1Jian-Ping SU 4Jian-Quan LIU 1(Key Laboratory of Evolution and Adaptation of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of sciences,Xining 810001,china) 2(Graduate university of the Chinese Academy of sciences,Beijing 100049,china) 3(school of life and Geography science,Qinghai Normal university,Xining 810008,china) 4(Key Laboratory of Arid and Grassland Ecology,school of life science,lanzhou university,lanzhou 730000,china) (Key Laboratory of Evolution and Adaptation of Plateau Biota Northwest Institute of Plateau Biology Chinese Academy of Sciences Xining 810001 China) (Graduate university of the Chinese Academy of sciences Beijing 100049 China) (school of life and Geography science Qinghai Normal University Xining 810008 China) (Key Laboratory of Arid and Grassland Ecology School of Life Science Lanzhou University Lanzhou 730000 China)

The Tibetan antelope(chiru,Pantholops hodgsoni),a heavily poached species and symbol of the QinghaiTibetan Plateau(QTP),is noted worldwide for its special calving *** species originated in the early Quaternary and it is interesting to know how the following climatic oscillations affected its demographic dynamics in the climate-sensitive *** this study,we analyzed the mitochondrial D-loop region from 312 individuals sampled in all of the six major *** found high rates of gene flow and little genetic differentiation between populations,suggesting that the calving migration may have homogenized the genetic pool of this *** mismatch distribution analyses and coalescent simulations suggested that this species experienced a demographic expansion approximately 600-200 Kyr following the retreat of the large glaciers developed in the QTP at 800-600 Kyr,rather than at the end of the last glacial age,as previously suggested,based on a limited sample *** addition,we found evidence of a chiru population decrease probably related to the human settings at the QTP during the middle Holocene.

关键词： chiru calving migration demographic expansion mitochondrial D-loop Quaternary.

甘肃省肃南县皇城区和民勤县治沙站动物多样性的比较研究

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西南师范大学学报（自然科学版） 2002年第1期27卷 88-97页

作者：戴怡龄兰州大学生命科学学院甘肃兰州730000

对分属高寒半干旱地带的肃南县皇城区和温带荒漠人工绿洲生境的民勤县治沙站进行了动物多样性调查 .分析比较的结果表明 :两地区共观察和采集到动物 141种 ,分属 8纲 2 6目 94科 .其中肃南县皇城区观察和采集到动物 81种 ,分属 6纲 18目... 详细信息

对分属高寒半干旱地带的肃南县皇城区和温带荒漠人工绿洲生境的民勤县治沙站进行了动物多样性调查 .分析比较的结果表明 :两地区共观察和采集到动物 141种 ,分属 8纲 2 6目 94科 .其中肃南县皇城区观察和采集到动物 81种 ,分属 6纲 18目 6 1科 ;脊椎动物和无脊椎动物的Simpson指数分别为 0 933和 0 95 6 ,Shannon -Wiener指数分别为 1 32 6和 1 396 .民勤县治沙站观察和采集到动物 87种 ,分属 8纲 2 3目 6 8科 ;脊椎动物和无脊椎动物的Simpson指数分别为 0 95 5和 0 96 1,Shannon Wiener指数分别为 1 391和 1 5 86 .提出了人工保护措施是增加物种多样性的一个影响因素 .

关键词：无脊椎动物脊椎动物生物多样性甘肃肃南县皇城区民勤县治沙站生物群落

Relationship Between Microbial Community and Soil Properties During Natural Succession of Abandoned Agricultural Land

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Pedosphere 2010年第3期20卷 352-360页

作者： JIA Guo-Mei ZHANG Pei-Dong WANG Gang CAO Jing HAN Jing-Cheng HUANG Ying-Ping College of Chemistry and life science China Three Gorges Unversity Yichang 443002 (China) Key Laboratory of Arid and Grassland Ecology School of Life Science Lanzhou University Lanzhou 730000 (China) Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao 266071 (China)

The changes of microbial biomass carbon (MBC) and nitrogen (MBN) and microbial community in the topsoil of the abandoned agricultural land on the semi-arid Loess Plateau in china during the natural succession were evaluated to understand the relationship between microbial community and soil properties. MBC and MBN were measured using fumigation extraction, and microbial community was analyzed by the method of fatty acid methyl ester (FAME). The contents of organic C, total N, MBC, MBN, total FAME, fungal FAME, bacterial FAME and Gram-negative bacterial FAME at the natural succession sites were higher than those of the agricultural land, but lower than those of the natural vegetation sites. The MBC, MBN and total FAME were closely correlated with organic C and total N. Furthermore, organic C and total N were found to be positively correlated with fungal FAME, bacterial FAME, fungal/bacterial and Gram-negative bacterial FAME. Natural succession would be useful for improving soil microbial properties and might be an important alternative for sustaining soil quality on the semi-arid Loess Plateau in china.

关键词： fatty acid methyl ester microbial biomass soil carbon soil nitrogen

生物学实验教学示范中心安全管理的探索与实践

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实验技术与管理 2009年第9期26卷 98-100页

作者：沈剑敏胡建成兰州大学生命科学学院

随着实验室流动学生人数的大幅增加,实验新设备的大量补充,且更加趋于集中,生物学实验教学示范中心的安全环保工作正在面临新的挑战。从实验中心的实际出发,对制定规章制度,普及安全教育,建立实验室安全预案,以及对废弃实验动物和有害... 详细信息

随着实验室流动学生人数的大幅增加,实验新设备的大量补充,且更加趋于集中,生物学实验教学示范中心的安全环保工作正在面临新的挑战。从实验中心的实际出发,对制定规章制度,普及安全教育,建立实验室安全预案,以及对废弃实验动物和有害废弃物的综合处理等安全管理工作进行了一些探索。

关键词：生物学实验教学示范中心安全管理管理制度实验室安全预案

稀土元素对L02细胞和SMMC-7721细胞的影响

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中国稀土学报 2005年第S2期23卷 107-110页

作者：陈凯高清祥张栩谢卓艺兰州大学生命科学学院甘肃兰州730000

为了研究稀土元素对正常肝细胞和肝癌细胞的低促高抑作用浓度的差别,采用MTT法测定CeCl3和PrCl3在不同浓度和不同培养基下对L02细胞和SMMC-7721细胞增殖的影响差异。结果表明:溶解稀土离子的培养液成分对细胞生长影响差异不大,不同稀土... 详细信息

为了研究稀土元素对正常肝细胞和肝癌细胞的低促高抑作用浓度的差别,采用MTT法测定CeCl3和PrCl3在不同浓度和不同培养基下对L02细胞和SMMC-7721细胞增殖的影响差异。结果表明:溶解稀土离子的培养液成分对细胞生长影响差异不大,不同稀土离子对L02细胞和7721细胞都有低促高抑的作用,但对7721细胞抑制的浓度比抑制L02细胞的浓度要大,因此高浓度稀土离子对正常肝细胞的毒害作用要比对肝癌细胞大。

关键词：肝细胞肝癌细胞 MTT 铈镨稀土

Effect of centrally administered C75,a fatty acid synthase inhibitor,on gastric motility in the conscious rats

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Effect of centrally administered C75,a fatty acid synthase i...

DEVELOPMENT OF POTENT Μ-OPIOID RECEPTOR LIGANDS USING NOVEL C-TERMINUS MODIFIED ANALOGS OF ENDOMORPHIN-2

中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Ai-Min YANG,Shuang-Yu LV,Ze-Yun MI,Yue-Ke JI,Shu-Fang SU,Qiang CHEN~* Institute of Biochemistry and Molecular Biology,school of life science,lanzhou university,lanzhou 730000,china

＜正＞C75,anα-methylene-γ-butyrolactone,is a synthetic inhibitor of fatty acid synthase（FAS）,based on the known mechanism of action of cerulenin and the theoretical reaction intermediates of theβ-ketoacyl synthase moiety of FAS.C75 treatment inhibited food intake and decreased body weight in *** evidence has accumulated to indicate that feeding behavior and fasted（inter-digestive） and the physiological fed（postprandial） motor activities in the digestive tracts are tightly *** have previously shown that centrally administered C75 delayed gastric emptying and gastrointestinal *** the present study,we investigated the effects of centrally administered C75 on gastric *** motility was monitored in freely moving conscious rats in the physiological fasted and fed states by using a miniature strain gauge transducer implanted in the stomach *** results indicated that when *** of C75 in the fasted state,the fasted patterns of gastric motility were disrupted and the fed-like motor patterns appeared,when *** of C75（10,32 and 56μg） was given to animals in the fed state,the%MI of the gastric contraction waves was significantly reduced in a dose-dependent *** addition,involvement of the NPY and vagal nerve in mediating the action of C75 was examined in the present *** showed that NPY and truncal vagotomy blocked the effects of *** of C75 on gastric motility,suggesting that the NPY and vagal pathway may mediate the action of centrally administered C75 on gastric *** conclusion,our findings indicate that centrally administration of C75 disrupted fasted motor patterns and inhibited fed motor activity in the antrum and that NPY and vagal nerve might be involved in these effects of C75 on gastric motility.

关键词： C75 gastric motility NPY vagal nerve

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DEVELOPMENT OF POTENT Μ-OPIOID RECEPTOR LIGANDS USING NOVEL...