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High-quality assembled and annotated genomes of Nicotiana tabacum and Nicotiana benthamiana reveal chromosome evolution and changes in defense arsenals

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Molecular plant 2024年第3期17卷 423-437页

作者： Jubin Wang Qingling Zhang Jeffrey Tung Xi Zhang Dan Liu Yingtian Deng Zhendong Tian Huilan Chen Taotao Wang Weixiao Yin Bo Lij Zhibing Lai savithramma p.dinesh-kumar Barbara Baker Feng Li National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China plant Gene Expression Center Department of Plant and Microbial BiologyUniversity of CaliforniaBerkeleyBerkeleyCA 94706USA College of plant Science and Technology Huazhong Agricultural UniversityWuhanHubei 430070China College of Life Science and Technology Huazhong Agricultural UniversityWuhanHubei 430070China Department of plant Biology and The Genome Center College of Biological SciencesUniversity of CalforniaDavisDavisCA 95616USA The Key Laboratory of Horticultural plant Genetic and Improvement of Jiangxi province Institute of Biological ResourcesJiangxi Academy of SciencesNanchang 330299China institute of Vegetables and Flowers Jiangxi Academy of Agricultural SciencesNanchang 330200Chin Hubei Hongshan Laboratory WuhanHubei 430070China

Nicotiana tabacum and Nicotiana benthamiana are widely used models in plant biology ***,genomic studies of these species have *** we report the chromosome-level reference genome assemblies for *** and *** with an estimated 99.5%and 99.8%completeness,*** transcription start and termination site sequencing methods were developed and used for accurate gene annotation in *** analyses revealed evidence for the parental origins and chromosome structural changes,leading to hybrid genome formation of each ***,theantiviral silencinggenesRDR1,RDR6,DCL2,DCL3,andAGO2were lost from one or both subgenomes in ***,while both homeologs were kept in ***,the *** genome encodes fewer immune receptors and signaling components than that of *** find-ings uncover possible reasons underlying the hypersusceptible nature of *** developed the user-friendly Nicomics(http:/***/Nicomics/)web server to facilitate better use of Nicotiana genomic resources as well as gene structure and expression analyses.

关键词： Nicotiana tabacum Nicotiana benthamiana genomics evolution disease resistance NLR

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Assessment of scale interactions associated with wake meandering using bispectral analysis methodologies

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Theoretical & Applied Mechanics Letters 2024年第2期14卷 65-75页

作者： dinesh kumar Kinjangi Daniel Foti Department of Mechanical Engineering University of MemphisMemphisTennesseeUSA

Large atmospheric boundary layer fluctuations and smaller turbine-scale vorticity dynamics are separately hypothesized to initiate the wind turbine wake meandering phenomenon,a coherent,dynamic,turbine-scale oscillation of the far *** interactions,the mechanism of energy transfers between scales,manifest as triples of wavenumbers or frequencies and can be characterized through bispectral *** bispectrum,which correlates the two frequencies to their sum,is calculated by two recently developed multi-dimensional modal decomposition methods:scale-specific energy transfer method and bispectral mode ***-eddy simulation of a utility-scale wind turbine in an atmospheric boundary layer with a broad range of large length-scales is used to acquire instantaneous velocity *** bispectrum from both methods identifies prominent upwind and wake meandering interactions that create a broad range of energy scales including the wake meandering *** coherent kinetic energy associated with the interactions shows strong correlation between upwind scales and wake meandering.

关键词： Wind turbine wake Mode decomposition Bispectral analysis

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Comparison of rare earths doping(neodymium and samarium) on structural and magnetic properties of Ni-Zn-Bi nanoferrites

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Journal of Rare Earths 2024年第3期42卷 534-542,I0004页

作者： Shilpa Taneja preeti Thakur dinesh kumar Yassine Slimani Atul Thakur NanoLatticeX Amity University HaryanaGururam122413HaryanaIndia Nanotechnology Wing Innovative Science Research SocietyShimla177002India Centre for Nanotechnology Amity University HaryanaGururam122413HaryanaIndia Department of Biophysics Institute for Research and Medical Consultations(IRMC)Imam Abdulrahman Bin Faisal UniversityP.O.Box 1982Dammam31441Saudi Arabia School of Electronics and Information Engineering Nanjing University of Information Science&TechnologyNanjing210044China

Excellent magnetic properties in ferrites are required for high-frequency applications and for wastewater treatment. Thus, the present study shows the comparison of magnetic and structural properties of Nd and Sm substituted Ni-Zn-Bi ferrites with the series Ni_(0.5)Zn_(0.5)Bi_(0.04)Nd_(x)Fe_(1.96-x)O_4(with step size 0.002)and Ni_(0.5)Zn_(0.5)Bi_(0.04)Sm_(x)Fe_(1.96-x)O_4(with step size 0.02) prepared using citrate precursor method. The impact of the substitution of rare earth ions(Nd and Sm) on magnetic properties of the synthesized samples is observed using a vibrating sample magnetometer(VSM). The saturation magnetization values enhance considerably from 52 to 58 emu/g for Nd^(3+)ions and 39 to 57 emu/g for Sm^(3+) ions, thus, making these materials magnetically hard. Further, the higher value of coercivity is also observed ranging from133 to 167 Oe for Nd^(3+)ions and 81 to 155 Oe for Sm^(3+) ions. The shape of hysteresis loops indicates a super paramagnetic and ferromagnetic behavior in the obtained samples. The squareness ratio value ispy of particles and hence, these ferrites are suitable for microwaveabsorbing and in permanent magnetic materials. The X-ray diffraction(XRD) pattern shows the formation of pure cubic crystallites, where, lattice parameters range from 0.840 to 0.839 nm and from 0.838to 0.839 nm for Nd^(3+)and Sm^(3+) ions substitution, respectively. The crystallite size ranges between 28.63to 29.89 nm and 18.33 to 26.23 nm, for substitution of Nd^(3+)and Sm^(3+) ions, respectively. Field emission scanning electron microscopy(FESEM) shows the formation of homogeneous grains, whereas, energy dispersive spectrometer(EDS) counts describe the purity of the samples. The Sm concentration x = 0.10has the maximum surface area with value of 42.6 m^(2)/g which proves to be having good data storage application due to high surface area. The zero-field cooled(ZFC) and field cooled(FC) data show that Nddoped Ni-Zn-Bi nano

关键词： Citrate precursor X-ray diffraction Vibrating sample magnetometer Coercivity Field emission scanning electron microscopy Rare earths

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proximity labeling: an emerging tool for probing in planta molecular interactions

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plant Communications 2021年第2期2卷 1-15页

作者： Xinxin Yang Zhiyan Wen Dingliang Zhang Zhen Li Dawei Li Ugrappa Nagalakshmi savithramma p.dinesh-kumar Yongliang Zhang State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology College of Biological SciencesChina Agricultural UniversityBeijing 100193P.R.China State Key Laboratory of plant physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Department of plant Biology and the Genome Center College of Biological SciencesUniversity of CaliforniaDavisDavisCA 95616USA

protein–protein interaction(ppI)networks are key to nearly all aspects of cellular ***,the identification of ppIs is important for understanding a specific biological process in an *** with conventional methods for probing ppIs,the recently described proximity labeling(pL)approach combined with mass spectrometry(MS)-based quantitative proteomics hasemerged as apowerful approach for characterizing ***,the application of pL in planta remains in its ***,we summarize recent progress in pL and its potential utilization in plant *** specifically summarize advances in pL,including the development and comparison of different pL enzymes and the application of pL for deciphering various molecular interactions in different organisms with an emphasis on plant systems.

关键词： protein interactions proximity labeling biotin ligase plant membrane contact sites organelles

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A plant Immune Receptor Adopts aDual Recognition Mechanism to Enhance pathogen Surveillance

A Plant Immune Receptor Adopts aDual Recognition Mechanism t...

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2018全国植物生物学大会

作者： Jia Li Haining Huang Min Zhu Shen Huang Wenhua Zhang savithramma p.dinesh-kumar Xiaorong Tao Department of plant pathology Nanjing Agricultural University College of Life Science Nanjing Agricultural University Department of plant Biology and The Genome Center College of Biological SciencesUniversity of California

plant intracellular nucleotide-binding leucine-rich repeat(NLR) immune receptors play critical roles in pathogen *** characterized plant NLRs recognize pathogen effectors in a single-step recognition *** demonstrate here that both the non-canonical N-terminal Solanaceae Domain(SD) and the NB-ARC-LRR region of tomato Sw-5b NLR immune receptor have evolved with unique roles to detect NSm effector and to activate defense *** Sw-5b SD directly interacts with NSm and

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Evaluation of Dielectric properties of CCTO-BT/Epoxy Composites for Electronic Applications

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Journal of Harbin Institute of Technology(New Series) 2024年第1期31卷 69-77页

作者： Swagatika Mishra punyapriya Mishra punyatoya Mishra dinesh kumar Mishra Krushna prasad Shadangi Deepak kumar Mohapatra Department of Mechanical Engineering Veer Surendra Sai University of TechnologyBurlaOdisha 768018India Department of physics Parala Maharaja Engineering CollegeBerhampurOdisha 761003India Department of Metallurgical and Materials Engineering Veer Surendra Sai University of TechnologyBurlaOdisha768018India Department of Chemical Engineering Veer Surendra Sai University of TechnologyBurlaOdisha 768018India

In the current study,the calcium copper titanate(CCTO)/epoxy,barium titanate(BT)/epoxy and CCTO-BT/epoxy composite samples with variable volume fractions of CCTO and BT are fabricated using hand lay-up and compression moulding process. The composite samples are characterized for the frequency dependence on dielectric properties,conductivity,impedance spectroscopy and electrical modulus.X-ray diffraction(XRD)representation of CCTO-BT/epoxy composite samples confirmed the presence of both CCTO and BT ceramic samples separately. The dielectric characteristics of hybrid CCTO-BT/epoxy composite samples with CCTO∶BT ratio of 40∶60, 60∶40,and 50∶50 was found relatively better than those of single ceramic filler reinforced epoxy composites. AC conductivity analysis shows improvement in the results of hybrid filler-filled CCTO-BT/epoxy composites in comparison with single filler-filled epoxy composite.50∶50 CCTO-BT/epoxy composite shows the best AC conductivity value of~ 2.2 ×10^(-5) ohm^(-1)·m^(-1) at a higher frequency of 1MHz. The impedance analysis confirms the higher insulating properties for hybrid 40∶60 and 60∶40 CCTO-BT/epoxy composites with respect to the single and other hybrid ceramic epoxy composites. The analysis suggests the hybrid CCTO-BT/epoxy composites to be adopted as a potential dielectric material for energy storage devices and other electronic applications.

关键词： ceramic filler dielectric characterization hybrid Composite AC conductivity impedance analysis

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Emergence of taurine as a therapeutic agent for neurological disorders

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Neural Regeneration Research 2024年第1期19卷 62-68页

作者： Ashok Jangra priyanka Gola Jiten Singh pooja Gond Swarnabha Ghosh Mahesh Rachamalla Abhijit Dey Danish Iqbal Mehnaz Kamal punya Sachdeva Saurabh kumar Jha Shreesh Ojha dinesh kumar Niraj kumar Jha Hitesh Chopra Shing Cheng Tan Department of pharmaceutical Sciences Central University of HaryanaMahendergarhHaryanaIndia Department of Biology University of SaskatchewanSaskatoonSKCanada Department of Life Sciences Presidency UniversityKolkataWest BengalIndia Department of Health Information Management College of Applied Medical SciencesBuraydah Private CollegesBuraydahSaudi Arabia Department of pharmaceutical Chemistry College of PharmacyPrince Sattam Bin Abdulaziz UniversityAl-KharjSaudi Arabia GloNeuro Vishwakarma RoadNoidaIndia Department of Biotechnology School of Engineering&Technology(SET)Sharda UniversityGreater NoidaUttar PradeshIndia Department of pharmacology and Therapeutics College of Medicine and Health SciencesUnited Arab Emirates UniversityAl AinUnited Arab Emirates School of Bioengineering&Biosciences Lovely Professional UniversityPhagwaraIndia Department of Biotechnology School of Applied&Life Sciences(SALS)Uttaranchal UniversityDehradunIndia Chitkara College of pharmacy Chitkara UniversityPunjabIndia UKM Medical Molecular Biology Institute Universiti Kebangsaan MalaysiaKuala LumpurMalaysia

Taurine is a sulfur-containing,semi-essential amino acid that occurs naturally in the *** alternates between inflammation and oxidative stress-mediated injury in various disease *** part of its limiting functions,taurine also modulates endoplasmic reticulum stress,Ca^(2+)homeostasis,and neuronal activity at the molecular *** effectively protects against a number of neurological disorders,including stro ke,epilepsy,cerebral ischemia,memory dysfunction,and spinal cord *** various therapies are available,effective management of these disorders remains a global *** 30 million people are affected *** design of taurine fo rmation co uld lead to potential drugs/supplements for the health maintenance and treatment of central nervous system *** general neuroprotective effects of taurine and the various possible underlying mechanisms are discussed in this *** article is a good resource for understanding the general effects of taurine on various *** the strong evidence for the neuropharmacological efficacy of taurine in various experimental paradigms,it is concluded that this molecule should be considered and further investigated as a potential candidate for neurotherapeutics,with emphasis on mechanism and clinical studies to determine efficacy.

关键词： antioxidant epilepsy γ-amino butyric acid neurodegenerative disorders neuroprotection oxidative stress spinal cord injury taurine

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predictive value of neutrophil-to-lymphocyte ratio in in-hospital mortality in sepsis patients:A cross-sectional study

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Journal of Acute Disease 2024年第3期13卷 106-110页

作者： pankaj kumar Jain Manoj Seval Vikas Meena dinesh kumar Meena Department of Internal Medicine Government Medical CollegeKotaIndia

Objective:To explore the predictive value of neutrophil-to-lymphocyte ratio(NLR)in in-hospital mortality in sepsis ***:A prospective observational cross-sectional study was conducted on 100 patients with *** data about the patient’s demography,medical history,general examination including pulse rate,blood pressure,etc,use of vasopressor support,need for renal replacement therapy,mechanical ventilation,outcome,and lab parameters including total lymphocyte count with neutrophil-to-lymphocyte ratio were *** parameters between survivals and non-survivals were ***:Out of 100 patients,80%were from rural *** patients were 50 to 59 years old.26 patients were *** patients in the nonsurvivor group were older and more had a history of diabetes mellitus when compared with the survivor *** non-survivor group had a higher NLR,ApACHE栻,and SOFA ***:NLR is a readily available parameter and can be used as a good prognostic indicator for mortality in sepsis patients.

关键词： Neutrophil-to-lymphocyte ratio Sepsis Intensive Care Unit Mortality ApACHE score SOFA score

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芸薹黄化病毒p0蛋白与植物SKp1互作有助于其自身的稳定性

芸薹黄化病毒P0蛋白与植物SKP1互作有助于其自身的稳定性

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中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者：李源源孙倩赵添羽向海英张晓艳吴占雨周翠姬王颖张永亮王献兵李大伟于嘉林 savithramma p.dinesh-kumar 韩成贵中国农业大学植物病理系与农业生物技术国家重点实验室加州大学戴维斯分校生物科学学院植物生物学系和基因中心

在植物中,RNA沉默是一种重要的抗病毒机制。植物通过切割病毒来源的双链RNA产生病毒来源的小干扰RNA(virus-derived small interfering RNA,vsiRNA),并由vsiRNA介导含有Argonaute(AGO)效应蛋白的RNA诱导的沉默复合体(RNA-induced silencing complex,RISC)对互补的病毒基因组进行切割。植物病毒在进化过程中也产生了一类称为病毒的RNA沉默抑制子(viral suppressor of RNA silencing,VSR)的序列和功能各异的蛋白。VSR通过不同的机制来抑制RNA沉默,其中一类能介导RNA沉默通路中关键效应蛋白的降解,如马铃薯卷叶病毒属p0蛋白被报道能介导AGO1蛋白的降解。马铃薯卷叶病毒属p0蛋白包含一个保守的类Fbox基序[LpXX(L/I)Xp]。与植物中的F-box蛋白类似,p0通过该基序与植物的SKp1-Cullin 1-F-box(SCF)复合体中的关键蛋白因子SKp1互作。前期研究表明,该基序的突变会导致p0的RNA沉默抑制功能丧失。但实际上是自噬通路参与了p0诱导的AGO1降解过程。因此,p0与SKp1蛋白互作的意义还有待进一步研究。芸薹黄化病毒(Brassica yellows virus,BrYV)是由本实验室首次发现并报道的马铃薯卷叶属新病毒。我们的研究表明BrYV p0蛋白(p0)是一个强的RNA沉默抑制子,能抑制由GFp在本生烟上诱发的局部和系统RNA沉默。通过对p0进行截短突变、关键基序定点突变和丙氨酸扫描突变,并对不同突变体的功能进行比较,我们鉴定了参与p0不同功能的氨基酸残基。和前人的结果类似,将p0类F-box基序中关键氨基酸进行丙氨酸替代突变(p0L63A/p64A,以下简称Lp)后,p0丧失了局部沉默抑制功能及与SKp1互作的能力。在对p0及其突变体蛋白的检测过程中发现,Lp突变体的积累量较野生型p0而言有明显的降低。共表达RNA沉默抑制子以及翻译效率验证的实验结果表明,Lp蛋白积累的减少可能是蛋白不稳定的结果,而抑制剂实验和TRV介导的VIGS实验则进一步证明了Lp蛋白积累量的降低是26S蛋白酶体和自噬降解通路共同作用的结果。随后,我们发现沉默本生烟中的SKp1同源基因(NbSKp1)能在不影响p0mRNA积累的情况下导致p0蛋白积累量的减少,暗示了p0与SKp1互作可能有助于维持自身的稳定性。此外,我们通过反向遗传学实验证明了p0主要通过介导AGO1降解来发挥其RNA沉默抑制功能,而p0与SKp1的互作并不是其抑制RNA沉默过程直接必需的,而可能是p0逃避植物的识别和降解以维持自身蛋白稳定的一种反防御机制。与这些结果相呼应,突变体病毒的接种实验表明,p0蛋白及其RNA沉默抑制能力是BrYV完成系统侵染所必须的。

关键词：马铃薯卷叶病毒属芸薹黄化病毒 p0 NbSKp1 互作自噬泛素化

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Study of Structural, Optical, Electrical and Dielectric properties of Gd-Doped ZnO Composites Synthesized by Solid State Reaction Method

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Open Journal of Composite Materials 2024年第2期14卷 91-108页

作者： Santosh kumar Kundara Kanhaiya Chawla dinesh kumar Yadav Chhagan Lal Balram Tripathi Narendra Jakhar Department of physics University of Rajasthan Jaipur India Sw. p.N.K.S. Government pG College Dausa India Government pG College Pratapgarh India S.S. Jain Subodh pG (Autonomous) College Jaipur India

In this manuscript, we are reporting structural, bonding, optical, dielectric, and electrical properties of Gd-doped ZnO composite samples (Zn1−xGdxO, x = 0, 0.05, 0.10) prepared by solid-state reaction method. XRD spectra confirm the wurtzite hexagonal phase with a grain size distribution of 42 - 47 nm. The FT-IR spectra confirm bonding behavior like Zn-O, O=C=O, and O-H stretching modes. FESEM micrographs show that the grains of crystallites possess nearly spherical morphology. Optical absorption spectra confirm that the optical band gap decreases systematically from 3.19 eV to 3.15 eV for x = 0.0 to x = 0.10 samples. For all samples, pL spectra exhibited near-band emission, blue emission, and green emission peaks. The dielectric constant decreases as the applied frequency increases. Hall effect results show that with increasing doping concentration of Gd, mobility and resistivity increase while bulk concentration decreases. Current-Voltage study shows that current increases when temperature is increased. Rare earth-doped ZnO is potential material used for optoelectronics and spintronics device applications. properties of Gd-doped ZnO are studied by various research groups, but dielectric studies are limitedly reported. Therefore, the present research work aims to study the change of electrical, optical, and dielectric properties of Gd-doped ZnO for device applications.

关键词： ZnO:Gd Composites Optical Band Gap Green Emission Dielectric Constant

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