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Preparation of Highly Concentrated Silver Nanoparticles in Reverse Micelles of Sucrose Fatty Acid Esters through Solid-Liquid Extraction Method

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Advances in Chemical Engineering and Science 2011年第4期1卷 299-304页

作者： Hidetaka Noritomi Yoshihiro Umezawa Saori Miyagawa satoru kato 不详

Silver nanoparticles were synthesized in reverse micelles consisting of sucrose fatty acid esters by dissolving reactant powder in the water pool of reverse micelles through the solid-liquid extraction. Silver nanoparticles having various sizes and shapes were obtained at high concentration. The size of silver nanoparticles was controlled by reaction temperature. Moreover, the size of silver nanoparticles was dependent upon the average esterification degree of sucrose fatty acid esters forming reverse micelles. The wavelength in the peaks, which corresponded upon the localized surface plasmon resonance of resultant silver nanoparticles, was correlated with their sizes.

关键词： Reverse Micelle Silver Nanoparticle Size Control Solid-Liquid Extraction Sucrose Fatty Acid Ester

Thermal stability of proteins in the presence of aprotic ionic liquids

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Journal of Biomedical Science and Engineering 2011年第2期4卷 94-99页

作者： Hidetaka Noritomi Ken Minamisawa Reo Kamiya satoru kato 不详

Thermal stability of lysozyme dissolved in aqueous solutions was examined in the presence of water-miscible aprotic ionic liquids consisting of 1-ethyl-3-methylimidazolium cation and several kinds of anions. Addition of ionic liquids to an aqueous solution containing lysozyme prevented unfolded proteins from aggregating irreversibly at high temperatures. The thermal denaturation curve of lysozyme with ionic liquids was entirely shifted to higher temperature, compared with that without ionic liquids. The remaining activity of lysozyme after the heat treatment was markedly dependent upon the kind and concentration of ionic liquids. The remaining activi-ties of lysozyme with 1.5 M 1-ethyl-3-methylimida-zolium tetrafluoroborate ([emim][BF4]) and 0.1 M 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([emim][Tf]) exhibited 88 and 68% after the heat treatment at 90oC for 30 min, respectively, although that without ionic liquids was perfectly lost.

关键词： Thermal Stability Lysozyme Ionic Liquid Remaining Activity

How can aprotic ionic liquids affect enzymatic enantioselectivity?

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Journal of Biomedical Science and Engineering 2013年第10期6卷 954-959页

作者： Hidetaka Noritomi Hideki Chiba Manabu Kikuta satoru kato Dai-Ichi Kogyo Seiyaku Co. Ltd. Oogawara-cho Kisshoin Kyoto Japan Department of Applied Chemistry Tokyo Metropolitan University Tokyo Japan

The enantioselectivity of α-chymotrypsin in the esterification of N-acetyl-tryptophan with ethanol was examined in aprotic ionic liquids. The enantioselectivity was found to be strongly affected by a kind of solvent, water content, and reaction temperature. The (kcatKM)L/(kcat/KM)D ratio in 1-ethyl-3-methylimidazolium bis(fluorosulfonyl)imide ([C2mim][FSI]) containing 1.0% (v/v) water at 25°C exhibits 32,000, while that in 1-ethyl-3-methylimidazolium tetrafluoroborate ([C2mim][BF4]) containing 1.0% (v/v) water at 25°C shows 190. The (kcat/KM)L/(kcat/KM)D ratio in [C2mim] [BF4] at 25°C varies from 190 at 1.0% (v/v) water to 65000 at 5.0% (v/v) water. Moreover, the (kcat/KM)L/ (kcat/KM)

关键词： α-Chymotrypsin Enantioselectivity Esterification Aprotic Ionic Liquid

Heat-Resistant Properties of <i>&#945</i>-Chymotrypsin Adsorbed onto Biomass Charcoal Powder

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Journal of Biomaterials and Nanobiotechnology 2014年第3期5卷 179-185页

作者： Hidetaka Noritomi Shunichi Kurihara Nobuyuki Endo satoru kato Department of Applied Chemistry Tokyo Metropolitan University Tokyo Japan Department of Applied Chemistry Tokyo Metropolitan University Tokyo Japan 2EEN Co. Ltd. Tokyo Japan EEN Co. Ltd. Tokyo Japan

Biomass charcoal powder (BCP) was used as a carrier for immobilization of α-chymotrypsin through adsorption. BCP was derived from plant biomass wastes such as dumped bamboos by oxygen-free pyrolysis at low temperatures and grinding with a jet mill. The activity of adsorbed α-chymotrypsin was strongly dependent upon the kind of BCP. The thermal denaturation curve of adsorbed α-chymotrypsin was shifted to high temperature, compared to that of free one. When α-chymotrypsin adsorbed onto BCP of bamboos was incubated at 45°C, the half-life of adsorbed α-chymotrypsin was 2.6 times greater than that of free one. After incubation at 45°C, the remaining activity of adsorbed α-chymotrypsin markedly depended on the kind of BCP.

关键词： Adsorption Biomass Charcoal Powder α-Chymotrypsin Activity Thermal Stability

The effect of molecular configuration of sterol on the liquid-ordered phase formation in DPPC/sterol mixed bilayers

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The effect of molecular configuration of sterol on the liqui...

Application of Reverse Micelles of Alkyl Glucosides to Synthesis of Silver Nanoparticles

第十七届国际生物物理大会;第十二届中国生物物理大会

作者： Tsubasa Miyoshi Hiroshi Kitajima satoru kato Graduate School of Science & Technology Kwansei Gakuin UniversityGakuen 2-1Sanda HyogoJapan

The lipid packing structure is one of the key factors for understanding the biomembrane *** is suggested to control the membrane properties through modification of the packing structures;it induces the relatively rigid liquid-ordered(Lo) phase when added to phospholipid bilayers in the liquid-disordered(Ld) ***,we are still far from full understanding of the formation mechanism of the cholesterol-induced

关键词： Liquid-ordered phase DPPC/cholesterol mixed bilayer Buoyant density measurements Specific volumes Cholesterol-analogues

来源： cnki会议评论

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Advances in Nanoparticles 2013年第4期2卷 344-349页

作者： Hidetaka Noritomi Saori Miyagawa Naohiro Igari Hiroaki Saito satoru kato Department of Applied Chemistry Tokyo Metropolitan University Hachioji Japan GUN EI Chemical Industry CO. LTD. Takasaki-shi Japan

Silver nanoparticles were prepared in reverse micelles of alkyl glucosides by the injection method. The TEM image showed that the obtained silver nanoparticles displayed a wide variety of shapes. The size of silver nanoparticles was strongly dependent upon the kind of reducing agents, and tended to slightly increase with an increase in reaction temperature. The size of silver nanoparticles was hardly dependent upon the molar ratio of water to surfactant and the alkyl chain length of surfactants. Silver nanoparticles having various shapes were obtained at high concentration by the solid-liquid extraction method. The resultant silver colloid could be preserved for at least one month without precipitation.

关键词： Silver Nanoparticle Reverse Micelle Alkyl Glucoside Injection Method Solid-Liquid Extraction Method

Formation and Solubilization Property of Water-in-Oil Microemulsions of Alkyl Glucosides

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Advances in Nanoparticles 2013年第4期2卷 366-371页

作者： Hidetaka Noritomi Yuki Ishida Tomokazu Yamada Hiroaki Saito satoru kato Department of Applied Chemistry Tokyo Metropolitan University Tokyo Japan GUN EI Chemical Industry CO. LTD. Takasaki-shi Japan

The dependence of solubilization properties on the alkyl chain length of alkyl glucosides (AG) in AG/isooctane/ n-butanol/water system was investigated. The stable Winsor II system consisting of a water-in-oil (w/o) microemulsion phase and an aqueous phase was formed in AG/isooctane/n-butanol/water system. The apparent critical micelle concentration of AG reverse micelles in organic phases was markedly dependent upon the alkyl chain length of AG. The limiting amount of solubilized water increased with an increase in the alkyl chain length of AG. The solubilization capacity of methyl orange (MO) was superior to that of methylene blue (MB), and the solubilization capacities of MO and MB tended to increase with increasing the alkyl chain length of AG. Reverse micelles of dodecyl glucoside (AG12) exhibited the significant solubilization capacities of cytochrome c and lysozyme, while ribonuclease A was not solubilized by AG12 reverse micelles.

关键词： Microemulsion Reverse Micelle Winsor II Alkyl Glucoside Solubilization Organic Dye Protein

Immobilization of Lysozyme on Biomass Charcoal Powder Derived from Plant Biomass Wastes

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Journal of Biomaterials and Nanobiotechnology 2012年第4期3卷 446-451页

作者： Hidetaka Noritomi Reona Ishiyama Ryotaro Kai Daiki Iwai Masahiko Tanaka satoru kato Department of Applied Chemistry Tokyo Metropolitan University Tokyo Japan EEN Co. Ltd. Tokyo Japan

Biomass charcoal powder (BCP) was used as a carrier matrix for immobilization of chicken egg white lysozyme. BCP was derived from plant biomass wastes such as dumped adzuki beans by pyrolysis without combustion under nitrogen atmosphere and grinding with a jet mill. The amount of lysozyme immobilized on BCP of adzuki beans by adsorption was 11 μmol/g (0.16 g/g) at pH 7.0. The optimum pH values for free and immobilized lysozyme activities were 6.8 and 7.2, respectively. The optimum temperature for both free and immobilized lysozyme activities was 25℃. The half-life of immobilized lysozyme exhibited 1.8-fold compared to that of free lysozyme at 5℃. Moreover, the half life of immobilized lysozyme was 7 times greater than that of lysozyme at 90℃.

关键词： Immobilization Biomass Charcoal Powder Lysozyme Activity Stability

Thermostability of lysozyme immobilized on biomass charcoal powder

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Thermostability of lysozyme immobilized on biomass charcoal ...