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Bioinspired synthesis of CVD graphene flakes and graphene-supported molybdenum sulfide catalysts for hydrogen evolution reaction

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Nano Research 2016年第1期9卷 249-259页

作者： Ke Chen Cong Li Zhaolong Chen Liurong Shi sathish reddy Huan Meng Qingqing Ji Yanfeng Zhang Zhongfan Liu Center for Nanochemistry (CNC) Beijing Science and Engineering Center for Low Dimensional Carbon Materials Beijing National Laboratory for Molecular Sciences College of Chemistry and Molecular Engineering Peking University Beijing 100871 China Key Laboratory for Special Functional Materials of Ministry of Education Henan University Kaifeng 475004 China Department of Materials Science and Engineering College of Engineering Peking University Beijing 100871 China

Chemical vapor deposition has been the most-promising approach for growing large-area high-quality graphene films on planar substrates. Beyond the lateral growth, the synthesis of three-dimensional （3D） graphene has also been demon- strated recently on metal foams and insulating nanoparticles for exploring their applications in electrochemical electrodes. However, the existing approaches need either to prefabricate abundant starting substrates, or to construct porous frameworks for graphene growth. Herein, we report a straightforward, bioinspired strategy for growing large-quantity graphene flakes on cuttlebone substrates using the chemical vapor deposition （CVD） method. The separated graphene flakes from growth substrates are highly crystalline and layer-thickness controllable, outperforming the traditional chemically exfoliated graphene with few surface groups. Due to their inheriting the biomineral-derived morphology, the 3D graphene microstructures show a highly exposed and curved surface, which can load more MoSx（x ≥ 2） catalysts than other planar supports for highly efficient hydrogen generation. Briefly, the bioinspired approach is expected to achieve a reasonable balance between quality and quantity for graphene production, thus propelling its wide applications in energy storage and conversion devices.

关键词： graphene bioinspired synthesis chemical vapor deposition three-dimensional hydrogen evolutionreaction

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Poly(amaranth) film based sensor for resolution of dopamine in the presence of uric acid:A voltammetric study

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Chinese Chemical Letters 2010年第12期21卷 1490-1492页

作者： Umesh Chandra B.E.Kumara Swamy Ongera Gilbert M.Pandurangachar sathish reddy S.Sharath Shankar B.S.Sherigara Department of P.G.Studies and Research in Industrial Chemistry Kuvempu UniversityJnana SahyadriShankaraghattaShimoga 577 451KarnatakaIndia Department of Chemistry SSMRV Degree CollegeJayanagara 4T BlockBanglore 41KarnatakaIndia

The electropolymerized film of amaranth was prepared on the surface of graphite pencil electrode（GPE） using cyclic voltammetric *** poly（amaranth） film coated electrode exhibited an excellent electrocatalytic activity towards the detection of dopamine（DA） in the presence of uric acid（UA） in 0.2 mol/L phosphate buffer solution at pH *** effect of interference study was carried out using differential pulse voltammetric *** poly（amaranth） modified GPE was applied for the detection of DA in dopamine injection with satisfactory results.

关键词： Poly(amaranth) Graphite pencil electrode Dopamine Uric acid Sensor

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Determination of Dopamine in Presence of Uric Acid at Poly (Eriochrome Black t) Film Modified Graphite Pencil Electrode

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American Journal of Analytical Chemistry 2011年第2期2卷 262-269页

作者： Umesh Chandra B. E. Kumara Swamy Ongera Gilbert sathish reddy B. S. Sherigara Department of PG Studies and Research in Industrial Chemistry Kuvempu University

A simple commercial graphite pencil electrode was used to investigate the electrochemical oxidation of dopamine. The electropolymerised film of eriochrome black t was prepared on the surface of graphite pencil electrode by using cyclic voltammetry technique. The prepared electrode exhibits an excellent electrocatalytical activity towards the determination of dopamine. The effects of concentration, pH and scan rate were investigated. Simultaneous detection of dopamine and uric acid was investigated by using both cyclic voltammetric and differential pulse voltammetry technique. The modified electrode was also used for the detection of dopamine in injection.

关键词： Poly (Eriochrome Black T) Graphite Pencil Electrode Dopamine Uric Acid Cyclic Voltammetry Differential Pulse Voltammetry

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Acute Toxicity of Three Dimensional Conformal Concurrent Chemoradiation with Dose Optimization for External Beam Radiation in Carcinoma Cervix—A Prospective Study

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International Journal of Medical Physics, Clinical Engineering and Radiation Oncology 2016年第3期5卷 204-211页

作者： sathish Balachandran Shyama Prem Sudha reddy KS Saravanan Kandasamy Department of Radiotherapy JIPMER Puducherry India Department of Radiotherapy MGMC Puducherry India Department of Medical Physics JIPMER Puducherry India

Aim: To prospectively evaluate the acute toxicity of 3D conformal radiotherapy with dose optimization in patients with carcinoma cervix. Materials and Methods: Carcinoma cervix patients stage IIB to IIIB (n = 30) treated during November 2011 to May 2013 at the institution with 3D conformal chemo-radiation were included in the study. They received weekly Cisplatin 40 mg/m2 for a maximum of 5 cycles. They received 46 Gy/23 fractions, 5 fractions per week of external beam radiation. In these patients dose optimization was done in order to achieve a tumor maximum dose (Dmax) around 105%. Various techniques were used for dose optimization which included the use of sub fields, adjusting the weightages, using wedges and the use of mixed energies. EBRT (External Beam Radiotherapy) was followed by two fractions of high dose rate intracavitary brachytherapy of 9 Gy each. Acute RTOG toxicity was assessed weekly during EBRT and 1 week post EBRT. Results: The median age of the patients was 45 (range: 30 - 55 years). All the patients completed EBRT;63.3% of the patients received all 5 cycles of chemotherapy while 26.6% of the patients received 4 cycles of chemotherapy and 10% of the patients received 3 cycles of chemotherapy. The most predominant toxicity seen was GI toxicity, diarrhea being the most common GI toxicity followed by vomiting. Neutropenia was the most common hematological toxicity. Most patients had grade 0 and grade 1 toxicity. None of the patients had grade 4 toxicity while few had grade 2 and 3 toxicity. Conclusion: 3D conformal concurrent chemo radiotherapy with Dmaxaround 105% reduces acute RTOG toxicity particularly grade 3 and 4 and improves patient compliance for concurrent chemo-radiotherapy.

关键词： 3D Conformal Dose Optimization Acute Radiation Toxicity

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A Hybrid Method for Magnetic Resonance Brain Images Classification and Segmentation Using Soft Computing Techniques

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Journal of Artificial Intelligence and Technology 2023年第3期3卷 134-141页

作者： Baireddy Sreenivasa reddy Anchula sathish Jawaharlal Nehru Technological University AnantapurAndhra PradeshIndia RGM College of Engineering and Technology NandyalAndhra PradeshIndia

Nowadays,brain tumor is a serious life-threatening disease that can often be treated with risky *** classification and segmentation methods for MR(magnetic resonance)brain images have been proposed,but the expected accuracy value could not be reached so *** this paper,we proposed a hybrid approach that includes modified fuzzy C-means and artificial neural network(ANN)*** consists of five stages:(a)noise removal,(b)feature extraction,(c)feature selection,(d)classification,and(e)***,a genetic optimized median filter is used to remove noise present in the input image,and then the essential features are extracted and selected using discrete wavelet transform and principle component analysis algorithms,*** normal and abnormal images are classified using the ANN ***,it is processed through a modified fuzzy C-means algorithm to segment the tumor portion *** proposed segmentation technique has been tested on the BRATS dataset and produces a sensitivity of 98%,Jaccard index of 97%,specificity of 98%,and accuracy of 95%.

关键词： ANN brain tumor DWT genetic optimized median filter modified fuzzy C-means PCA

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Honey vesicle-like nanoparticles protect aged liver from non-alcoholic steatohepatitis

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Acta Pharmaceutica Sinica B 2024年第8期14卷 3661-3679页

作者： Baolong Liu Phuong Linh Nguyen Han Yu Xingzhi Li Huiren Wang Tram Gia Bao Nguyen Prakash Kumar Sahoo Meghna Sur Jay reddy Sarah Sillman Stephen D.Kachman Bara Altartouri Guoqing Lu sathish Kumar Natarajan Mahesh Pattabiraman Jiujiu Yu Department of Nutrition and Health Sciences University of Nebraska-LincolnLincolnNE 68583USA Shaanxi Key Laboratory of Molecular Biology for Agriculture College of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China Department of Nutrition Case Western Reserve UniversityClevelandOH 44106USA School of Veterinary Medicine and Biomedical Sciences University of Nebraska-LincolnLincolnNE 68583USA Department of Statistics University of Nebraska-LincolnLincolnNE 68583USA Center for Biotechnology University of Nebraska-LincolnLincolnNE 68588USA Department of Biology University of Nebraska OmahaOmahaNE 68182USA Department of Chemistry University of Nebraska-KearneyKearneyNE 68849USA

Non-alcoholic steatohepatitis(NASH),an advanced form of non-alcoholic fatty liver disease(NAFLD),has emerged as the leading cause of liver failure and related ***,no medication is specifically approved to treat NAFLD or *** we report that oral administration of honey vesiclelike nanoparticles(H-VLNs)to naturally aged mice protects the liver from NASH development.H-VLNs are dominantly taken up by Kupffer cells in the liver and suppress hepatic chronic inflammation and further development of fibrosis and nodule formation in aged *** their reported antiinflammasome function,H-VLNs are found to inhibit the transcriptional activities of C-JUN and nuclear factor-kappa B(NF-κB).MicroRNAs miR5119 and miR5108 and phenolic compound luteolin in H-VLNs are identified in suppressing both the C-JUN and NF-kB ***,oral intake of H-VLNs represents a promising new user-friendly modality to prevent the development of NASH.

关键词： Honey Extracellular vesicles Exosomes Nanoparticles microRNAs Aging Inflammation Non-alcoholic steatohepatitis

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Transcriptional Expression Profile of Toll Like Receptor 1 - 10 mRNA in Bovine Peripheral Mononuclear Cells in Response to Foot and Mouth Disease Antigens

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Advances in Microbiology 2012年第4期2卷 417-425页

作者： sathish Gaikwad Sowmya Kumar Thimmareddy Prashanth Golla Rama reddy Veluvarthy Sanyasi Suryanarayana Hosur Joyappa Dechamma Foot and Mouth Disease Research Laboratory Indian Veterinary Research Institute Bangalore Campus Bangalore India Foot and Mouth Disease Research Laboratory Indian Veterinary Research Institute Izatnagar India

Toll like receptors are the primary component of innate immune response. The comparison of expression of innate immune genes TLR 1 - 10 was carried out by real time PCR by quantifying mRNA in bovine PBMCs. The bovine PBMCs in vitro stimulated with FMD infectious virus lead to up regulation of TLR 2, 3, 4 and down regulation of TLR 9. But stimulation with DNA vaccine carryingVP1 genes of FMDV leads to up regulation of TLR 2 only. Difference in the TLR gene expression by the virus and DNA vaccine, may be related to the outcome of the vaccine and carrier or persistent state of the animal which is seen in FMDV.

关键词： Innate Immunity FMDV DNA Vaccine TLR Genes

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Synthesis,isolation,identification and characterization of new process-related impurity in isoproterenol hydrochloride by HPLC,LC/ESI-MS and NMR

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Journal of Pharmaceutical Analysis 2017年第6期7卷 394-400页

作者： Neeraj Kumar Subba Rao Devineni Prasad reddy Gajjala Shailendra Kumar Dubey Pramod Kumar Chemical Research Department Micro Labs LtdAPI DivisionBommasandra-Jigani Link RoadKIADB INDL AreaBommasandraBangalore 560105KarnatakaIndia

One unknown impurity （Imp-II） during the analysis of laboratory batches of isoproterenol hydrochloride was detected in the level ranging from 0.04% to 0.12% by high performance liquid chromatography with UV detection. The unknown impurity structure was proposed as 4-[2-（propan-2oylamino）ethyl]benzene-l,2-diol （Imp-II） using the liquid chromatography-mass spectrophotometry （LC-MS） analysis. Imp-II was isolated by semi-preparative liquid chromatography from the impurity-enriched reaction crude sample. Its proposed structure was confirmed by nuclear magnetic spectroscopy such as 1H, 13C, DEPT （1D NMR）, HSQC （2D NMR） and infrared spectroscopy （IR）, and retention time and purity with HPLC followed by the chemical synthesis. Due to less removable nature of Imp-II during the purification, the synthetic process was optimized proficiently to control the formation of Imp-II below to the limit 〈 0.12% in the course of reaction. The new chemical route was developed for the preparation of this impurity in required quantity with purity to use as reference standard. The most probable mechanism for the formation of Imp-II was discussed in details.

关键词： Isoproterenol hydrochloride Impurities HPLC NMR

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Transcriptional Expression Profile of Toll Like Receptor 1-10 mRNA in Bovine Peripheral Mononuclear Cells in Response to Foot and Mouth Disease Antigens

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Advances in Microbiology 2012年第4期

作者： sathish Gaikwad Sowmya Kumar Thimmareddy Prashanth Golla Rama reddy Veluvarthy Sanyasi Suryanarayana Hosur Joyappa Dechamma

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