检索结果-南通市图书馆

Optimization of Chromate Reduction by Whole Cells of <i>Arthrobacter</i>sp. SUK 1205 Isolated from Metalliferous Chromite Mine Environment

引用

Geomaterials 2012年第4期2卷 73-81页

作者： satarupa dey Amal K. Paul Microbiology Laboratory Department of Botany University of Calcutta Kolkata India

Arthrobacter sp. SUK 1205 isolated from metalliferous chromite mine environment of Orissa, India showed wide degree of tolerance to heavy metals including Cr(VI), variety of antibiotics and was also capable of reducing Cr(VI) during growth. Freshly grown whole cells of this bacterium were evaluated for chromate reduction under batch culture using Vogel Bonner (V. B.) broth as the base. Cells of SUK 1205 were capable of completely reducing 100 μM Cr(VI) in V. B. broth within 48 h of incubation. Reduction of chromate increased with increase in cell density which attained maximum at 1010 cells/ml, however, reverse was the phenomenon when the concentration of Cr(VI) increased gradually. Glycerol, glycine and glucose promoted chromate reduction efficiency of the cells when used as electron donors. Optimum pH and temperature were found to be 7.0 and 35°C respectively. The process of reduction was inhibited by Ni(II), Mn(II), Zn(II) and Co(II), but Cu(II) and Fe(III) was promotive in nature. On the other hand, 2, 4-dinitrophenol was found to be neither promotive nor inhibitory for the reduction process, but carbonyl cyanide-m-chloro phenyl hydrazone, sodium azide, sodium fluoride and N,N,-dicyclohexyl carboiimide were inhibitory. Cells of SUK 1205 when permeabilized with toluene, triton X-100 and tween 80 showed an enhancement of the process and thereby indicated that reduction of Cr(VI) was mainly associated with soluble component of the cells. Arthrobacter sp. SUK 1205, therefore, showed great promise for use in Cr(VI) detoxification under a wide range of environmental conditions.

关键词： Arthrobacter sp. Chromate Reduction Chromite Mine Overburden Detoxification Hexavalent Chromium Metal Resistance Bioremediation Environmental Pollution

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Occurrence and Evaluation of Chromium Reducing Bacteria in Seepage Water from Chromite Mine Quarries of Orissa, India

引用

Journal of Water Resource and Protection 2010年第4期2卷 380-388页

作者： satarupa dey Amal K. Paul 不详

Chromium pollution due to leaching and weathering of chromite mine overburden in mine seepage water is a serious environmental problem. In an attempt to explore the indigenous chromate resistant and reducing bacteria from chromite mine quarry seepage, 145 aerobic, heterotrophic bacteria were isolated from 14 mine seepage samples derived from chromite mines of Sukinda valley and Baula-Nuasahi belt in Orissa, India. A total of 109 isolates which were tolerant to 2 mM Cr(VI) showed different degrees of Cr(VI) reducing activ-ity in complex KSC medium and synthetic Vogel Bonner (VB) broth. Ten isolates belonging to genera like Pseudomonas, Acinetobacter, Alcaligenes, Cupriavidus and Corynebacterium were selected on the basis of their chromate reducing efficiency and it was found that they could reduce more than 50 and 75% of Cr(VI) in VB broth and KSC medium respectively. The sole Gram-positive isolate, Corynebacterium paurometabo-lum SKPD 1204 (MTCC 8730) showed maximum chromate reducing capacity in both VB broth (63.7%) and KSC medium (92%) and was capable of reducing nearly 95% of the total Cr(VI) in the mine seepage when it was grown in the mine seepage supplemented with 2% VB concentrate.

关键词： Chromite Mine Seepage Hexavalent Chromium Chromium Resistant Bacteria Chromate Re-duction Corynebacterium paurometabolum Heavy Metal Tolerance Antibiotic Sensitivity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：