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Developmental DNA methyltransferase expression in the fire ant Solenopsis invicta

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Insect Science 2018年第1期25卷 57-65页

作者： sasha Kay Daniel Skowronski Brendan G. Hunt Department of Entomology University of Georgia Griffin Georgia USA

DNA methylation is accomplished in animals by 2 classes of enzymes known as DNA methyltransferases, DNMT3 and DNMT 1, which perform de novo methylation and maintenance methylation, respectively. Several studies of hymenopteran eusocial insects suggest that DNA methylation is capable of influencing developmental plasticity. However, fundamental questions remain about the patterning of DNA methylation during the course of insect development. In this study, we performed quantitative real-time PCR （qPCR） on transcripts from the single-copy orthologs of DNMT1 and DNMT3 in the red imported fire ant, Solenopsis invicta. In particular, we assessed the expression of S. invicta Dnmtl and Dnmt3 mRNA during 7 stages of worker development, among behaviorally distinct adults, and among male and female gonads. Dnmt3 was most highly expressed during embryonic development, whereas Dnmtl was similarly expressed throughout the course of develop- ment. Moreover, Dnmtl and Dnmt3 were highly expressed in testes and ovaries. Neither Dnmt was significantly differentially expressed among heads of behaviorally distinct adult castes. Our results support the hypothesis that extensive patterning of DNA methylation occurs during gametogenesis and embryogenesis in the insect order Hymenoptera.

关键词： DNA methylation epigenetics gene expression Hymenoptera qPCR Solenopsis invicta

A mini review on the separation of Al,Fe and Ti elements from coal fly ash leachate

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International Journal of Coal Science & Technology 2024年第2期11卷 1-15页

作者： Yuan Shi Fengqi Jiang Rongjiao Wang sasha Yang Xiaofeng Zhu Yingying Shen State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals School of Material Science and EngineeringLanzhou University of TechnologyLanzhou 730050China Department of Chemical and Biological Engineering Monash UniversityWellington RoadClaytonMelbourneVIC 3800Australia

The electricity demand is increasing rapidly with the development of society and ***-fired thermal power plants have become one of the primary sources of electricity generation for ***,coal-fired thermal power plants produce a great amount of by-product coal fly ash every *** fly ash disposal in landfills requires a sizable space and has negative environmental ***,it is crucial to develop new technologies and methods to utilize this enormous volume of solid waste in order to protect the *** this review,the fundamental physical and chemical character-istics of coal fly ash are introduced,and afterward the disposal policies and utilization ways of coal fly ash are discussed to gain a comprehensive understanding of the various ways this *** leaching of valuable metals in coal fly ash and the extraction of metal elements in leachate under different conditions are also ***,the possibility of coal fly ash to serve as a supplementary source for mineral resources is analyzed,providing a basis for its extensive use as a raw material in the metal industry in China and worldwide.

关键词： Coal fly ash Waste utilization Metals extraction

Highly efficient and automated extraction of DNA from human remains using a modified EZ1 protocol

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Forensic Sciences Research 2021年第1期6卷 59-66页

作者： Anna Barbaro sasha Samar Giacomo Falcone Angelo La Marca Department of Forensic Genetics Studio Indagini Mediche E Forensi(SIMEF)Reggio CalabriaItaly

Bones and teeth often represent the only sources of DNA available for identifying human *** in bones and teeth is generally better preserved than that in soft tissues because of the presence of hard connective tissue with a high level of *** of the extensive mineralisation,the choice of an efficient DNA extraction procedure is important to minimise the sampling of a high level of minerals and to remove polymerase chain reaction(PCR)*** protocols are available for DNA extraction from bones and teeth as part of the Qiagen EZ1 DNA Investigator Kit using the EZ1 Advanced XL automated purification *** improve the efficiency of DNA extraction from skeletal remains,the present study focuses on a modification to these already available *** this study,different bones and teeth collected between 1 and 50 years after death were subjected to DNA extraction using the standard EZ1 protocol,a supplementary protocol,and a modified *** modified approach included a decalcification step,whereas the Qiagen protocols worked directly on non-decalcified *** all three procedures,150 mg samples were used for DNA *** evaluated the quantity of DNA recovered from samples,the presence of any PCR inhibitors co-extracted,the level of DNA degradation,the quality of short tandem repeat(STR)profiles,and the reproducibility of the modified *** compared with the other protocols,the modified protocol resulted in the best recovery of DNA that was free of PCR ***,the STR profiles were reliable and of high *** our opinion,the decalcification step increases DNA recovery by softening tissues,which allows lysis solutions to act more ***,the use of two lysis solutions and the variation added to the EZ1 purification step allow for DNA recovery with quality and quantity superior to those of the previously available Qiagen-based *** findings may be helpful solutions to t

关键词： Forensic sciences forensic genetics DNA extraction bone tooth EZ1 automation

Effects of calcium on the evolution of nitrogen during pyrolysis of a typical low rank coal

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International Journal of Coal Science & Technology 2020年第2期7卷 397-404页

作者： Xiongchao Lin sasha Yang Xujun Chen Panpan Zheng Yonggang Wang Shu Zhang School of Chemical and Environmental Engineering China University of Mining and Technology(Beijing)Beijing100083China Department of New Energy Science and Technology Nanjing Forestry UniversityNanjing210037JiangsuChina

This study aims to investigate the effects of calcium on the migration of nitrogen in coal(coal-N)to N-containing gas species,particularly,NH3 and HCN(volatile-N)in volatiles,as well as the chemical transformation of the N in char during coal pyrolysis under different *** pyrolysis experiments of Shengli brown coal and its derived coal samples loaded with different contents of calcium were conducted under 600–800°C in a novel fluidized bed *** experimental results showed that during coal pyrolysis,the generation of NH3 is mainly derived from secondary reactions among volatiles,tar and char with the catalytic effect of mineral matter,especially calcium in *** pyrolysis temperature from 600 to 800°C could enhance the release of N in coal to ***,the increased pyrolysis temperature could also inhibit the generation of NH3 while facilitating the formation of *** release of HCN is more sensitive to pyrolysis ***,under higher pyrolysis temperatures,more N-containing structures in coal would become thermally unstable and crack into HCN;On the other hand,higher pyrolysis temperature could also enhance the decomposition of N in coal to N-containing species in tar or N2,thus reducing the release of HCN and *** in tar could either undergo secondary decomposition reactions,generating NH3,HCN,N2 and other N-containing species in gas phase,or experience condensation polymerization by forming macromolecular structure and be retained in char at high pyrolysis *** could significantly restrain the release of N from coal,thus reducing the yields of NH3 and *** coal pyrolysis,calcium catalytically enhances the fracture and combination of chemical bonds,generating abundant free *** free radicals could continuously attack N-containing structures and consequently release the N-containing gaseous products,such as NH3,HCN,N2 etc.,resulting in the decrease of N in ***

关键词： Brown coal Pyrolysis Nitrogen Calcium Transformation

Advances in the treatment of cervical rheumatoid: Less surgery and less morbidity

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World Journal of Orthopedics 2014年第3期5卷 292-303页

作者： Grant W Mallory sasha R Halasz Michelle J Clarke Department of Neurologic Surgery Mayo ClinicRochesterMN 55905United States

Rheumatoid arthritis is a chronic systemic inflammatory disease that often affects the cervical *** it was initially thought that cervical involvement was innocuous,natural history studies have substantiated the progressive nature of untreated *** the past 50 years,there has been further elucidation in the pathophysiology of the disease,as well as significant advancements in medical and surgical *** introduction of disease modifying drugs and biologic agents has reduced the amount of patients with advanced stages of the disease needing *** in instrumentation techniques has improved patient outcomes and fusion *** introduction of endoscopic approaches for ventral decompression may further lower surgical *** this review,we give a brief overview of the pertinent positives of the disease.A discussion of historical techniques and the evolution of surgical therapy into the modern era is *** improved medical therapies and lessinvasive approaches,we will likely continue to see less advanced cases of disease and less surgical ***,a thorough understanding of the disease is crucial,as its systemic involvement and need for continued medical therapy have tremendous impact on overall complications and outcomes even in patients being seen for standard degenerative disease with comorbid rheumatoid.

关键词： Atlantoaxial instability Cranial settling Subaxial subluxation Cervical Surgery Morbidity Rheumatoid arthritis

The Age of Neuroeducation

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基础教育外语教学研究 2015年第7期 30-34页

作者： sasha Euler

Most educators will, at some point in their professional lives, have experienced unmotivated students with no interest in the subject, or even in learning in general, students who resent school and who would rather be anywhere else but in *** a consequence, many teachers can be heard to complain at length about how unpleasant their job is and how difficult it is to deal with students today—never considering that they might just be the primary cause of the situation.

关键词：英语学习学习方法阅读知识阅读材料

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Keep on

疯狂英语（初中天地） 2020年第11期 21-23页

作者： sasha Sloan 不详

歌手独特的嗓音很温柔空灵,听她的歌很治愈,很舒服.你只需要在糟糕的日子里依然满怀希望,你只需要沉着呼吸,你只需要继续奋勇向前……

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Prognostic model of survival outcomes in non-small cell lung cancer patients initiated on afatinib: pooled analysis of clinical trial data

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Cancer Biology & Medicine 2019年第2期16卷 341-349页

作者： Ashley M. Hopkins Adel Shahnam sasha Zhang Chris S. Karapetis Andrew Rowland Michael J. Sorich Flinders Centre for Innovation in Cancer Department of Clinical Pharmacology College of Medicine and Public Health Flinders University Adelaide 5042 Australia The Canberra Hospital Garran 2605 Australia

Objective: Several predictors of survival have been identified in EGFR-positive non-small cell lung cancer(NSCLC) patients treated with first generation EGFR inhibitors. Prognostic models of survival outcomes with afatinib have not been ***: A prognostic tool for overall survival(OS)/progression free survival(PFS) based on pre-treatment clinicopathological factors was developed for EGFR-positive advanced NSCLC patients treated with first-line afatinib using penalised regression of individual-participant data from LUX-Lung 3 and 6(n = 468). Favourable, intermediate and poor risk groups were identified and externally validated using LUX-Lung 1(n = 390) and LUX-Lung 2(n = 129) trials that initiated afatinib following previous chemotherapy or EGFR inhibitor ***: Discriminative performance was good in the development and validation cohorts. For patients treated with first-line afatinib, the median OS for the favourable, intermediate and poor risk groups were > 47.7, 29.3 and 16.4 months, respectively, and the median PFS were 17.3, 13.2 and 8.3 months, respectively. The improvement in median OS with afatinib use compared to chemotherapy was > 12.4 months for the favourable risk group, whereas no OS benefit was apparent for the poor risk group. The improvement in median PFS with afatinib use compared to chemotherapy was 10.2 months for the favourable risk group and 3.2 months for the poor risk ***: A prognostic tool was developed and validated to identify favourable, intermediate and poor risk groups for OS/PFS in EGFR-positive advanced NSCLC patients treated with afatinib. The prognostic groups can inform the likely absolute OS/PFS benefit expected from afatinib compared to chemotherapy in first-line treatment.

关键词： Afatinib non-small cell lung cancer prognostic model

Liquid biopsies to predict CDK4/6 inhibitor efficacy and resistance in breast cancer

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Cancer Drug Resistance 2022年第3期5卷 727-748页

作者： sasha C.Main David W.Cescon Scott V.Bratman Princess Margaret Cancer Centre University Health NetworkToronto M5G 2C1OntarioCanada Department of Medical Biophysics University of TorontoToronto M5G 1L7OntarioCanada Division of Medical Oncology and Hematology Department of MedicineUniversity of TorontoToronto M5S 1A8OntarioCanada Department of Radiation Oncology University of TorontoToronto M5T 1P5OntarioCanada

Cyclin-dependent kinase 4 and 6(CDK4/6)inhibitors combined with endocrine therapy have transformed the treatment of estrogen receptor-positive(ER+)and human epidermal growth factor receptor 2 negative(HER2-)metastatic breast ***,some patients do not respond to this treatment,and patients inevitably develop resistance,such that novel biomarkers are needed to predict primary resistance,monitor treatment response for acquired resistance,and personalize treatment *** the spatial and temporal limitations of tissue biopsy,newly developed liquid biopsy approaches have the potential to uncover biomarkers that can predict CDK4/6 inhibitor efficacy and resistance in breast cancer patients through a simple blood *** on circulating tumor DNA(ctDNA)-based liquid biopsy biomarkers of CDK4/6 inhibitor resistance have focused primarily on genomic alterations and have failed thus far to identify clear and clinically validated predictive biomarkers,but emerging epigenetic ctDNA methodologies hold promise for further *** present review outlines recent advances and future directions in ctDNA-based biomarkers of CDK4/6 inhibitor treatment response.

关键词： Breast cancer liquid biopsy circulating tumor DNA cell-free DNA CDK4/6 inhibitors resistance mechanisms predictive biomarkers circulating biomarkers