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Chemometrics of differentially expressed proteins from colorectal cancer patients

World Journal of Gastroenterology 2011年第16期17卷 2096-2103页

作者： Lay-Chin Yeoh saravanan dharmaraj Boon-Hui Gooi Manjit Singh Lay-Harn Gam School of Pharmaceutical Sciences Universiti Sains Malaysia Centre for Drug Research Universiti Sains Malaysia Department of Surgery Penang General Hospital

AIM:To evaluate the usefulness of differentially expressed proteins from colorectal cancer (CRC) tissues for differentiating cancer and normal ***:A Proteomic approach was used to identify the differentially expressed proteins between CRC and normal *** proteins were extracted using Tris buffer and thiourea lysis buffer (TLB) for extraction of aqueous soluble and membrane-associated proteins,***,namely principal component analysis (PCA) and linear discriminant analysis (LDA),were used to assess the usefulness of these proteins for identifying the cancerous state of ***:Differentially expressed proteins identified were 37 aqueous soluble proteins in Tris extracts and 24 membrane-associated proteins in TLB *** on the protein spots intensity on 2D-gel images,PCA by applying an eigenvalue > 1 was successfully used to reduce the number of principal components (PCs) into 12 and seven PCs for Tris and TLB extracts,respectively,and subsequently six PCs,respectively from both the extracts were used for *** LDA classification for Tris extract showed 82.7% of original samples were correctly classified,whereas 82.7% were correctly classified for the cross-validated *** LDA for TLB extract showed that 78.8% of original samples and 71.2% of the cross-validated samples were correctly ***:The classification of CRC tissues by PCA and LDA provided a promising distinction between normal and cancer *** methods can possibly be used for identification of potential biomarkers among the differentially expressed proteins identified.

关键词：化学计量学方法蛋白质组学差异表达大肠癌组织状态可溶性蛋白患者线性判别分析

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Effect of Dilution of Treated Distillery Effluent (TDE) on Soil Properties and Yield of Sugarcane

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American Journal of Plant Sciences 2013年第9期4卷 1811-1814页

作者： Previna Sivaloganathan Baskar Murugaiyan saravanan Appavou Leninraja dharmaraj Department of Soil Science and Agricultural Chemistry Anbil Dharmalingam Agricultural College and Research Institute Trichirapalli India.

Sugarcane is one of the most important cash crops, which plays a pivotal role in India’s agricultural and industrial economy. The treated distillery effluent (TDE) being plant originated, contains all plant nutrients and organic matter. Therefore, it is being used as a cheap source of nutrients and organic manure in agriculture activities in soil besides improving soil physical properties. The experiment was conducted during August, 2010-2011 and 2011-2012 with CO 86032 in a randomized block design with six treatments and was replicated four times. The TDE was discharged @ 1.00, 0.50, 0.33, 0.25 and 0.20 lakh liters·ha-1 to get the dilutions of 1:10, 1:20, 1:30, 1:40 and 1:50 dilutions respectively. It was applied four times at 40 days interval starting from 45th day after planting. The fertilizers viz., N and P @ 75 percent of the recommended dose were applied and K was skipped. The results revealed that irrigation with TDE at 1:10 dilution resulted in higher yield of sugarcane. The TDE did not have any influence on quality parameters of sugarcane. The TDE application favourably influenced the available nutrients and organic carbon content in the soil. Besides, the present findings credibly proved that the TDE application not only enhanced the soil fertility status but also substituted for 25 percent of N and P and 100 percent of K fertilizers to sugarcane crop.

关键词： TDE Dilution Sugarcane Yield Soil Properties

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