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Biometric Analysis on Genetic Divergence between Parental and Regenerated Accessions in Tall Coconut Palms (Cocos nucifera L.) from International Genebank for Africa and the Indian Ocean

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Open Journal of Genetics 2022年第2期12卷 11-23页

作者： saraka didier martial yao Wentoin Alimata Marie Pierre Daramcoum Eric-Blanchard Zadjéhi Koffi Konan Jean Louis Konan Nafan Diarrassouba Bourdeix Roland Raoul Sylvère Sie Irié Arsène Zoro Bi Department of Biochemistry and Genetics Pedagogical and Research Unit (UPR) of Genetics Shea Breeding Program University of Peleforo Gon Coulibaly (UPGC) Korhogo C&#244 te d’Ivoire Centre National de Recherche Agronomique (CNRA) Marc Delorme Research Station of Port-Bou&#235 t Abidjan C&#244 te d’Ivoire CEFE-CNRS Montpellier France Department of Natural Science Nangui Abrogoua University Abidjan C&#244 te d’Ivoire

This study endeavour assesses agromorphological likeness between initial introductions and regenerated accessions at the International Coconut Genebank for Africa and the Indian Ocean (ICG-AIO) based in C?te d’Ivoire. Ten couples of parental (G0) and regenerated (G1) accessions of Tall coconut palms were analyzed using Principal Component Analysis (PCA) and Multiple Analysis of Variance (MANOVA) from 26 agromorphological characters. The main results showed a relative decrease in the expression of the phenotypical traits concerning the component of the fruit, height and vigor of the stem and yield of bunches and fruits after one regeneration cycle. But, a high proportion (69%) of studied characters from leaf, inflorescence and nut components showed likeness between G0 and G1 accessions. After one regeneration cycle, the controlled pollination method guarantees significant conservation of the expression of the majority of agromorphological traits. Consequently, regenerated accessions of Tall coconut palms can be used to pursue research and development programs in C?te d’Ivoire.

关键词： Agromorphological Likeness Controlled Pollination Côte d’Ivoire Regenerated Accessions Tall Coconut

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Integration between Genomic and Computational Statistical Surveys for the Screening of SNP Genetic Variants in Inflammatory Bowel Disease (IBD) Pediatric Patients*

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Computational Molecular Bioscience 2024年第3期14卷 146-191页

作者： Dago Dougba Noel Koffi N’Guessan Bénédicte Sonia Dagnogo Olefongo Daramcoum Wentoin Alimata Marie-Pierre Mauro Giacomelli Dagnogo Dramane Eboulé Ago Eliane Rebecca yao saraka didier martial Diarrassouba Nafan Giovanni Malerba Raffaele Badolato Department of Clinical and Experimental Sciences University of Brescia Brescia Italy Angelo Nocivelli Institute of Molecular Medicine Childrens Hospital ASST Spedali Civili Brescia Italy Unit of Biostatistics and Biomathematics & Unit of Bioinformatics Department of Molecular and Translational Medicine University of Brescia Brescia Italy Biological Sciences Training and Research Unit Department of Genetic and Biochemistry Peleforo Gon Coulibaly University Korhogo Cte dIvoire Department of Neurosciences Biomedicine and Movement Sciences Section of Biology and Genetics University of Verona Verona Italy Biosciences Training and Research Unit Biology and Health Laboratory Felix Houphouet-Boigny University Abidjan Cte dIvoire

Inflammatory bowel diseases (IBD) are complex multifactorial disorders that include Crohn’s disease (CD) and ulcerative colitis (UC). Considering that IBD is a genetic and multifactorial disease, we screened for the distribution dynamism of IBD pathogenic genetic variants (single nucleotide polymorphisms;SNPs) and risk factors in four (4) IBD pediatric patients, by integrating both clinical exome sequencing and computational statistical approaches, aiming to categorize IBD patients in CD and UC phenotype. To this end, we first aligned genomic read sequences of these IBD patients to hg19 human genome by using bowtie 2 package. Next, we performed genetic variant calling analysis in terms of single nucleotide polymorphism (SNP) for genes covered by at least 20 read genomic sequences. Finally, we checked for biological and genomic functions of genes exhibiting statistically significant genetic variant (SNPs) by introducing Fitcon genomic parameter. Findings showed Fitcon parameter as normalizing IBD patient’s population variability, as well as inducing a relative good clustering between IBD patients in terms of CD and UC phenotypes. Genomic analysis revealed a random distribution of risk factors and as well pathogenic SNPs genetic variants in the four IBD patient’s genome, claiming to be involved in: i) Metabolic disorders, ii) Autoimmune deficiencies;iii) Crohn’s disease pathways. Integration of genomic and computational statistical analysis supported a relative genetic variability regarding IBD patient population by processing IBD pathogenic SNP genetic variants as opposite to IBD risk factor variants. Interestingly, findings clearly allowed categorizing IBD patients in CD and UC phenotypes by applying Fitcon parameter in selecting IBD pathogenic genetic variants. Considering as a whole, the study suggested the efficiency of integrating clinical exome sequencing and computational statistical tools as a right approach in discriminating IBD phenotypes as well as improvi

关键词： Inflammatory Bowel Disease (IBD) Crohn Disease (CD) Ulcerative Colitis (UC) Clinical Exome Analysis Computational Statistic SNP Genetic Variants

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Design of bifunctional single-atom catalysts NiSA/ZIF-300 for CO_(2) conversion by ligand regulation strategy

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Nano Research 2024年第5期17卷 3827-3834页

作者： Wengang Fu Yapei Yun Hongting Sheng Xiaokang Liu Tao Ding Shuxian Hu Tao yao Binghui Ge Yuanxin Du didier Astruc Manzhou Zhu Department of Chemistry and Centre for Atomic Engineering of Advanced Materials Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of EducationCollege of Materials Science and EngineeringInstitutes of Physical Science and Information Technology and Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized MaterialsAnhui UniversityHefei 230601China Universitéde Bordeaux ISMUMR CNRS N°5255351 Cours de La Libération33405 Talence CedexFrance National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefei 230026China Department of Physics University of Science and Technology BeijingBeijing 100083China Anhui Tongyuan Environment Energy Saving Co. LtdHefei 230041China

Carbon-supported noble-metal-free single-atom catalysts(SACs)have aroused widespread interest due to their green chemistry aspects and excellent ***,we propose a“ligand regulation strategy”and achieve the successful fabrication of bifunctional SAC/MOF(MOF=metal-organic framework)nanocomposite(abbreviated NiSA/ZIF-300;ZIF=ZIF-8)with exceptional catalytic performance and *** designed NiSA/ZIF-300 has a planar interfacial structure with the Ni atom,involving one S and three N atoms bonded to Ni(Ⅱ),fabricated by controllable pyrolysis of volatile Ni-S *** CO_(2) cycloaddition to styrene epoxide,NiSA/ZIF-300 exhibits ultrahigh activity(turnover number(TON)=1.18×105;turnover frequency(TOF)=9830 molSC·mol_(Ni)^(-1)·h^(-1);SC=styrene carbonate)and durability at 70℃ under 1 atm CO_(2) pressure,which is much superior to Ni complex/ZIF,NiNP/ZIF-300,and most reported *** study offers a simple method of bifunctional SAC/MOF nanocomposite fabrication and usage,and provides guidance for the precise design of additional original SACs with unique catalytic properties.

关键词： single atom catalyst bifunctional CO_(2)conversion cycloaddition

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Outcomes of Hemodialysis Patients in Public Centres in Abidjan from September 2018 to October 2021: Data from the Renal Registry of Cote d’Ivoire

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Open Journal of Nephrology 2024年第3期14卷 413-426页

作者： Sery Patrick Diopoh Arthur Kanganga Serge didier Konan Kolo Claude Ouattara Marie-Dominique Kouadio Mohamed Alex Moudachirou Kouamé Hubert yao Department of Medicine and Medical Specialties University Flix Houphout-Boigny Abidjan Cote dIvoire National Centre for Prevention and Treatment of Renal Failure Abidjan Cte dIvoire

Background: Hemodialysis is the most widely used renal replacement therapy in developing countries such as Cote d’Ivoire. Objective: To study the outcome of chronic hemodialysis patients in public centres in Abidjan using data from the Renal Registry of Cote d’Ivoire (2RCI). Method: This was a prospective analytical cohort study of End Stage Kidney Disease (ESKD) patients undergoing iterative hemodialysis in the network of public hemodialysis centres in Abidjan and who had been registered in the 2RCI register by September 2018. Results: Our study involved 363 cases out of 379 patients registered in the 2RCI registry database in 2018. The mean age of the patients was 47.3 ± 12.1 years, with a male predominance (sex ratio 1.97). In 70.24% of cases, they had no health insurance. The average duration of dialysis for our patients was 6.94 ± 4.1 years, with extremes of 2 and 28 years. This duration was less than 5 years in 44.3% of cases, between 6 and 10 years in 42.9% and more than 10 years in 12.6% of cases. The comorbidities found were hypertension in 95.6% of cases, diabetes in 7.8% and HIV in 4.6%. Anemia was found in 68% of cases and stroke in 8.6%. During the study period, 161 deaths were observed, representing a mortality rate of 44.35%. Acute lung oedema (29.8%), stroke (6.8%) and other cardiovascular diseases (19.3%) were the main causes of death. In multivariate analysis, factors such as age ≥ 65 years (HR = 3.66;CI 95% = 1.55 - 8.67;p = 0.003), “married/coupled” status (HR = 2.02;CI 95% = 1.24 - 3.31;p = 0.005) and normal weight at the start of dialysis (OR = 9.59, CI95% = 4.19 - 21.95;p = 0.001) were associated with the risk of death. Conclusion: Hemodialysis is performed in Abidjan on young patients. The mortality rate after three years of dialysis is very high. Hence the need to pursue the policy of decentralizing public centres and optimizing access to dialysis in terms of quality and quantity in order to improve patient survival.

关键词： Chronic Hemodialysis Death Renal Registry Cote d’Ivoire

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Low Birth Weight and Intermittent Preventive Treatment of Malaria in Pregnant Women in Lomé (Togo) in 2021: A Cross-Sectional Study

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Open Journal of Epidemiology 2024年第1期14卷 31-44页

作者： Roméo Mèdéssè Togan Ounoo Elom Takassi Fifonsi Gbeasor-Komlanvi Arnold Junior Sadio Rodion yao Konu Martin Kouame Tchankoni Iwone Oumarou Adama Latame Komla Adoli Dzayissé Yawo Atakouma didier Koumavi Ekouévi Department of Public Health Faculty of Health Sciences University of Lomé Lomé Togo African Center for Research in Epidemiology and Public Health (CARESP) Lomé Togo Training and Research Center in Public Health University of Lomé Lomé Togo Department of Pediatrics Faculty of Health Sciences University of Lomé Lomé Togo Global Health in the Global South (GHIGS) Team Insem UMR 1219 IRD EMR 271 University of Bordeaux Bordeaux France

Background: Since 2012, the World Health Organization has recommended intermittent preventive treatment with sulfadoxine-pyrimethamine (IPT-SP) to prevent malaria-related complications in pregnant women. Ten years following these recommendations, we conducted this study to estimate the coverage for three doses of IPT-SP (IPT3) as well as the prevalence of low birth weight (LBW), and its associated factors in Lomé (Togo) in 2021. Methods: A cross-sectional study was conducted between January and March 2021. An exhaustive recruitment of women and their newborns was carried out in the maternity wards of the Sylvanus Olympio University Hospital Center. Data from antenatal consultations and clinical data of the newborns were collected. Multivariate logistic regression was carried out to determine factors associated with LBW. Results: A total of 252 mother-child pairs were included in this study. Median age of the mothers was 27 years, IQR [24-31]. More than a third (35.3%) of the mothers were primigravida. IPT3 coverage was 66.7% and 14.7% of newborns had a LBW. The prevalence of LBW was 33.3% [23.3-43.4] in women who had received fewer than 3 doses of IPT-SP and 5.4% [2.0-8.8] in those who had received at least 3 doses of IPT-SP (p Conclusion: Ten years following recommendations of the WHO on IPT-SP, malaria prevention based on IPT-SP is not optimal among pregnant women in Lomé, and the proportion of LBW children remains high. Actions to strengthen the three-dose IPT-SP policy are needed to prevent malaria and its consequences among newborns in Togo.

关键词： Malaria Intermittent Preventive Treatment Low Birth Weight Togo

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COVID-19 Vaccination Status and Severe Outcomes among Patients Hospitalized for COVID-19 in West Africa, Togo, 2021

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Advances in Infectious Diseases 2024年第1期14卷 87-104页

作者： Awèréou Kotosso yao Rodion Konu Lidaw Déassoua Bawe sarakawabalo Assenouwe Yawovi Mawufemo Tsevi Koffi Atsu Aziagbe Akouda Akessiwe Patassi Bawoubadi Abaltou Gnimdou Tchamdja Lampouguini Nebona Komi Séraphin Adjoh didier Ekouevi Majesté Ihou Wateba Faculté des Sciences de la Santé Université de Lomé Lomé Togo Centre Hospitalier Régional Lomé Commune Lomé Togo Service des Maladies Infectieuses Centre Hospitalier Universitaire Sylvanus-Olympio Lomé Togo Unité de Recherche Mixte 1219 Bordeaux Population Health Centre University of Bordeaux National Institute for Health and Medical Research (INSERM) Research Institute for Sustainable Development (IRD) Bordeaux France Département de Santé Publique Université de Lomé Lomé Togo Faculté des Sciences de la Santé Université de Kara Kara Togo Service d’Anesthésie et Réanimation Centre Hospitalier Universitaire Kara Kara Togo Service de Néphrologie et d’Hémodialyse Centre Hospitalier Universitaire Sylvanus-Olympio Lomé Togo Service de Pneumo-Phtisiologie Centre Hospitalier Universitaire Sylvanus-Olympio Lomé Togo

Objectives: More than a year after its introduction, COVID-19 vaccination coverage was low in the Togolese population and little data were available on its benefits for hospitalized patients. This study aimed to describe the impact of COVID-19 vaccination on the prognosis of hospitalized patients. Methods: This was a retrospective cohort study of patients admitted to the Centre Hospitalier Régional Lomé Commune (Togo) between June 1, 2021 and May 31, 2022. Primary outcomes (admission to the intensive care unit and death) were presented with frequency and proportion. Mortality rates were presented by sociodemographic and clinical characteristics and compared by appropriate statistical tests. Factors associated with inpatient death were described by performing a Cox proportional hazard regression. Results: A total of 604 patients were hospitalized (50.0% women). The mean age was 54.03 ±17.1 years. Only 55 patients were fully vaccinated (9.1%). ICU admission was significantly more frequent in unvaccinated patients than in vaccinated ones (63.0% vs. 38.2%;p Conclusion: COVID-19 vaccination had a significant benefit for patients with COVID-19 infection in terms of reducing the risk of death. Based on real-world data from sub-Saharan Africa, this information can help optimize the benefit of COVID-19 vaccination by raising community awareness and increasing vaccine coverage while reducing hesitancy.

关键词： COVID-19 Vaccination Prognosis Mortality Sub-Saharan Africa

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Evaluation of the Productivity of Intercropping Plantain Cultivar (PITA 3) Fertilized with Two Types of Manure, under Coconut Tree Based (Cocos nucifera L.), on the Tertiary Sands of Côte d’Ivoire

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Agricultural Sciences 2023年第10期14卷 1405-1419页

作者： Pierre-Marie Janvier Coffi Joséphine Tamia Ama Thierry Tacra Lekadou Siaka Traore Charly Fernand Agoh didier martial saraka yao Geosciences and Environment Laboratory Environmental Sciences and Management Training and Research Unit (UFR) Nangui Abrogoua University (UNA) Abidjan C&#244te d’Ivoire Centre National de Recherche Agronomique Station de Recherche Marc DELORME Abidjan C&#244te d’Ivoire Department of Biochemistry-Genetics UFR of Biological Sciences Péléforo Gon Coulibaly University Korhogo C&#244te d’Ivoire

Pressure on land tenure is having a negative impact on the coconut sector, reducing farmers’ incomes. Intercropping cultivars plantain under coconut based has been considered as a solution to this problem. The aim of this work is to diversify the sources of income for coconut growers. The plantain variety PITA 3, popularised by the CNRA, was grown in coconut inter-rows (PB113+), with two types of manure (chemical, organic). Six (06) treatments D1, D2, D3, D4, D5 and D6 were studied. In the tenth month after planting, treatment D3, which included banana plants fertilised with 9 kg of manure/plant, got the best agromorphological performance: height (264.08 cm), neck circumference (57.68 cm) and 12 leaves. In terms of production parameters, D3 banana plants had a shorter production cycle (347 days) and the highest diet mass (9.3 kg). However, the plants that received no fertiliser (D6) showed stunted growth and were unable to produce brunch. The fertilization of plantain with 10 t/ha of laying hen droppings permitted good development and production of plantains on tertiary sands.

关键词： Intercropping Coconut Plantain PITA 3 Manure Tertiary Sands

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Multiple Antihypertensive Therapy in Nephrology Practice

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Open Journal of Nephrology 2017年第3期7卷 80-93页

作者： yao Kouame Hubert Doro Arouna Guehi Monlet Cyr Konan Serge didier Gnionsahe Daze Appolinaire Department of Nephrology Treichville Teaching HospitalAbidjanCote d'Ivoire

Introduction: Hypertension (HT) can be the cause or consequence of chronic kidney disease. Its management often requires a multiple therapy due to its severity. Objective: To describe the profile of patients receiving a multiple anti-hypertensive therapy in nephrology practice. Materials and Methods: This was a prospective, descriptive study conducted in the department of Nephrology, Yopougon Teaching Hospital, from January 1 to October 31, 2016. We included all patients admitted to this department who had received at least three anti-hypertensive drugs. Results: Out of a total of 625 hypertensive patients admitted over the study period, we included 120 patients on multiple therapy, i.e. a 19% prevalence. HT was essential in 60% of cases, secondary to chronic glomerulonephritis (CGN) in 25%, to diabetes in 13.3% and to polycystic kidney disease (PKD) in 1.7%. The therapy consisted of the combination of 3 antihy-pertensive drugs in 36.7% of cases, 4 drugs in 49.2% and 5 drugs in 4.2%. The antihypertensive classes used were Calcium channel blockers (CCB) in 99.2% of cases, Diuretics (D) in 87.5%, Angiotensin Converting Enzyme Inhibitors (ACEI) in 70%, Centrally acting medication (C) in 66.7%, Angiotensin Receptor Blockers (ARB) in 25.8% and Beta-blockers (β-) in 6.7%. The main combinations were CCB + D + ACEI + C in 34.2% of cases, CCB + D + ACEI in 23.3%, and CCB + D + ARB + C in 12.5%. The combinations of antihypertensive drugs varied according to the cause of HT with a non-significant difference. Patient outcome was characterized by normal blood pressure in 64.2% of cases and normal renal function in 13.3%. The mortality rate was 17.5%. In multivariate analysis, stage 5 renal disease (p = 0.001), hypertensive retinopathy (p = 0.04) and hemoglobin level < 8 g/dl (p = 0.039) were associated with mortality. Conclusion: Multiple antihypertensive therapy, which is common in nephrology, is related to the severity of HT and not to its cause. We still use centrally acting

关键词： Hypertension Multiple Therapy Nephrology

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Massive Envenomation by Bees Sting in a Child in Togo

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Open Journal of Pediatrics 2016年第3期6卷 232-236页

作者： Djatougbe Ayaovi Elie Akolly Koffi Mawuse Guedenon Enyonam Tsolenyanu Lidi Kama Bessi didier Koffi Gnamey yao Atakouma Chu Sylvanus Olympio Lome Tago

Attacks by swarm of bees realize arrays of mass poisoning. We report the case of a 9 years old boy whoes severity was related to the large number of bees which was about 400 and their locations spread throughout the skin surface with a preponderance to the cephalic end. Complications were hemolytic anemia, kidney failure and convulsions. The outcome was favorable with symptomatic care and hemodialysis.

关键词： Bee Envenomation Renal Failure Child Togo

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First Emergency Hemodialysis Session at the Nephrology Department of the Teaching Hospital of Yopougon: About 146 Cases

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Open Journal of Nephrology 2020年第4期10卷 338-347页

作者： Konan Serge didier Kissou Pegdebamba François Guei Monlet Cyr Diopoh Sery Patrick Aka Jean Astrid yao Kouamé Hubert Nephrology-Internal Medicine Department D CHU Treichville Abidjan Ivory Coast Department of Nephrology CHU Yopougon Abidjan Ivory Coast

Context: Due to the late referral of the pads to the nephrologist and the lack of medical follow-up, many patients are admitted with complications from kidney disease requiring the urgent start of hemodialysis sessions. Purpose: Study the profile of emergency hemodialysis patients in order to ease their management. Methods: This was a retrospective, descriptive and analytical study carried out at the Nephrology Department of the Teaching Hospital of Yopougon from January 1st to December 31st, 2016. This study included all patients who had received a first session of hemodialysis in an emergency context. Results: We collected 146 patients with an average age of 39.80 ± 14.55 years and a sex ratio of 0.6 for men. Before admission, patients were known as hypertensives (63.9%), followed by CKD (23.9%) and HIV-infected (8.2%). The main clinical signs were hypertension (64.3%), edema (44.5%) and coma (30.1%). Anemia was observed in 97.2% of cases and it was less than 8 g/dl in 57.5%. Kidney failure was chronic in 75.3% and acute in 24.6%. Chronic nephropathies were glomerular (54.1%), vascular (20.5%). The main indications for hemodialysis were encephalopathy (33.5%), severe uremia (28%), acute pulmonary edema (19.8%), persistent anuria (11.6%) and hyperkalemia (5.4%). The vascular approach was a catheter in 97.2% (femoral site in 53.4% and jugular in 43.8% and arteriovenous fistula in 2.7%). Death was observed in 17.8%. In univariate analysis, age > 65 years (p = 0.04), coma (p = 0.004) and acute kidney failure (p = 0.02) were associated with the risk of death, and in multivariate analysis, only coma (p = 0.024, OR = 5.88) seemed to be associated with mortality risk for our patients. Conclusion: Hemodialysis in an emergency situation is a common practice in the Teaching Hospital of Yopougon and mainly concerns patients with CKD.

关键词： Kidney Failure Hemodialysis Emergency

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