检索结果-南通市图书馆

Hymenopteran-specific TRPA channel from the Texas leaf cutter ant (Atta texana) is heat and cold activated and expression correlates with environmental temperature

在线全文

学校读者我要写书评

暂无评论

INSECT SCIENCE 2025年第1期32卷 301-320页

作者： York, Julia M. Taylor, Timothy N. LaPotin, sarah Lu, Ying Mueller, Ulrich Univ Illinois Dept Evolut Ecol & Behav Urbana IL USA Univ Texas Austin Dept Integrat Biol Austin TX USA Univ Texas Austin Inst Neurosci Austin TX USA Univ Utah Dept Human Genet Salt Lake City UT USA Univ Illinois Dept Evolut Ecol & Behav Urbana IL 61801 USA

Leaf cutting ants of the genus Atta cultivate fungal gardens, carefully modifying environmental conditions to maintain optimal temperature for fungal growth. Antennal nerves from Atta are highly temperature sensitive, but the underlying molecular sensor is unknown. Here, we utilize Atta texana (Texas leaf cutter ant) to investigate the molecular basis of ant temperature sensation and how it might have evolved as the range expanded northeast across Texas from ancestral populations in Mexico. We focus on transient receptor potential (TRP) channel genes, the best characterized temperature sensor proteins in animals. Atta texana antennae express 6 of 13 Hymenopteran TRP channel genes and sequences are under a mix of relaxed and intensified selection. In a behavioral assay, we find A. texana workers prefer 24 C-degrees (range 21-26 C-degrees) for fungal growth. There was no evidence of regulatory evolution across a temperature transect in Texas, but instead Hymenoptera-specific TRPA (HsTRPA) expression highly correlated with ambient temperature. When expressed in vitro, HsTRPA from A. texana is temperature activated with Q10 values exceeding 100 on initial exposure to temperatures above 33 C-degrees. Surprisingly, HsTRPA also appears to be activated by cooling, and therefore to our knowledge, the first non-TRPA1 ortholog to be described with dual heat/cold activation and the first in any invertebrate.

关键词： electrophysiology evolution invertebrate leaf cutter ants temperature TRP channels

Ecological immunology: do sexual attraction and immunity trade-off through a desaturase?

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

INSECT SCIENCE 2025年第1期32卷 290-300页

作者： Gao, Ke van der Heide, Wout Muijderman, daphne Nichols, sarah Karwal, Carmen Kuperus, Peter Groot, Astrid T. Univ Amsterdam Inst Biodivers & Ecosyst Dynam Amsterdam Netherlands Univ Calif San diego Dept Cell & Dev Biol La Jolla CA USA Cornell Univ Dept Neurobiol & Behav Ithaca NY USA Leiden Univ Leiden Netherlands

Given the limited availability of resources in nature, sexual attractiveness may trade off with immunocompetence, as the immunocompetence handicap hypothesis (ICHH) posits. In invertebrates, a direct link between trade-offs through hormonal/molecular effectors in sexual signals and immunity has not been found so far. Here, we assessed how variation in sexual signals affected parasite infection in two sex pheromone selected lines of the moth Chloridea virescens: an attractive line with a low ratio of 16:Ald/Z11-16:Ald and an unattractive line with a high ratio. When infecting these lines with an apicomplexan parasite, we found that the attractive Low line was significantly more susceptible to the parasite infection than the unattractive High line. Since the ratio difference between these two lines is determined by a delta-11-desturase, we hypothesized that this desaturase may have a dual role, i.e., in the quality of the sexual signal as well as an involvement in immune response, comparable to testosterone in vertebrates. However, when we used CRISPR/cas9 to knockout delta-11-desturase in the attractive Low line, we found that the pheromonal phenotype did change to that of the High line, but the infection susceptibility did not. Notably, when checking the genomic location of delta-11-desaturase in the C. virescens, we found that mucin is adjacent to delta-11-desaturase. When comparing the mucin sequences in both lines, we found four nonsynonymous SNPs in the coding sequence, as well as intronic variation between the two lines. These differences suggest that genetic hitchhiking may explain the variation in susceptibility to parasitic infection.

关键词： Chloridea virescens delta-11-desaturase ICHH parasite susceptibility sex pheromone

Altered microRNA composition in the uterine lumen fluid in cattle(Bos taurus)pregnancies initiated by artificial insemination or transfer of an in vitro produced embryo

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2025年第1期16卷 76-91页

作者： Fernando H.Biase sarah E.Moorey Julie G.Schnuelle Soren Rodning Martha Sofia Ortega Thomas E.Spencer School of Animal Sciences Virginia Polytechnic Institute and State University175 W Campus DrBlacksburgVA 24061USA department of Animal Science University of TennesseeKnoxvilleTN 37996USA department of Clinical Sciences Auburn UniversityAuburnAL 36849USA department of Animal Science Auburn UniversityAuburnAL 36849USA department of Animal and dairy Sciences University of Wisconsin MadisonMadisonWI 53706USA division of Animal Sciences University of MissouriColumbiaMO 65211USA

Background MicroRNAs(miRNAs)are presented in the uterine lumen of many mammals,and in vitro experiments have determined that several miRNAs are important for the regulation of endometrial and trophoblast *** aim was to identify and contrast the miRNAs present in extracellular vesicles(EVs)in the uterine lumen fluid(ULF)at the onset of attachment in cattle pregnancies(gestation d 18)initiated by artificial insemination(AI)or by the trans-fer of an in vitro-produced blastocyst(IVP-ET).A third group had no conceptus after the transfer of an IVP *** The abundance of 263 annotated miRNAs was quantified in the EVs collected from *** was an increase in the transcript abundance of 20 miRNAs in the ULF EVs from the AI pregnant group,while 4 miRNAs had a lower abundance relative to the group not containing a ***,4 miRNAs were more abun-dant in ULF EVs in the AI pregnant group relative to IVP-ET group(bta-mir-17,bta-mir-7-3,MIR7-1,MIR18A).Specific miRNAs in the ULF EVs were co-expressed with messenger RNAs expressed in extra-embryonic tissues and endome-trium,including genes that are known to be their *** The results provide biological insights into the participation of miRNAs in the regulation of trophoblast proliferation and differentiation,as well as in endometrium *** knowledge that in vitro cultured embryos can contribute to the altered abundance of specific miRNAs in the uterine lumen can lead to the development of cor-rective approaches to reduce conceptus losses during the first month of pregnancy in cattle.

关键词： Artificial reproductive technology Cattle Conceptus Embryos Endometrium Small RNA

Tailored design of novel Co0-Coδ+dual phase nanoparticles for selective CO2 hydrogenation to ethanol

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2025年第3期149卷 598-615页

作者： Subhasis das Ganapati d.Yadav department of Chemical Engineering Institute of Chemical TechnologyNathalal Parekh MargMatunga 400019MumbaiIndia

Catalytic hydrogenation of CO_(2)to ethanol is a promising solution to address the greenhouse gas(GHG)emissions,but many current catalysts face efficiency and cost *** based catalysts are frequently examined due to their abundance,cost-efficiency,and effectiveness in the reaction,where managing the Co^(0)to Co^(δ+)ratio is *** this study,we adjusted support nature(Al_(2)O_(3),MgO-MgAl_(2)O_(4),and MgO)and reduction conditions to optimize this balance of Co^(0)to Co^(δ+)sites on the catalyst surface,enhancing ethanol *** selectivity of ethanol reached 17.9%in a continuous flow fixed bed micro-reactor over 20 mol%Co@MgO-MgAl_(2)O_(4)(CoMgAl)catalyst at 270°C and 3.0 MPa,when reduced at 400°C for 8 *** results coupled with activity analysis confirmed that mild reduction condition(400°C,10%H_(2)balance N_(2),8 h)with intermediate metal support interaction favoured the generation of partially reduced Co sites(Co^(δ+)and Co^(0)sites in single atom)over MgO-MgAl_(2)O_(4)surface,which promoted ethanol synthesis by coupling of dissociative(CHx^(∗))/non-dissociative(CHxO^(∗))intermediates,as confirmed by density functional theory ***,the CoMgAl,affordably prepared through the coprecipitation method,offers a potential alternative for CO_(2)hydrogenation to yield valuable chemicals.

关键词： CO_(2)hydrogenation Ethanol Co-based catalysts Metal support ineraction dFT analysis

Analyzing the Cytotoxic and Genetic Impact of datura stramonium Extract on MCF7 and HT29 Cancer Cells:A Metabolite and Gene Expression Study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2025年第1期94卷 181-198页

作者： Fayez Althobaiti Hadeer darwish Raghad Alruqayb Saqer S.Alotaibi Fahad E.Alharthi Ibrahim Jafri sarah Awwadh Altalhi Ghadi Alsharif Hussam Awwadh E Althagafi Hesham Noureldeen sarah Alharthi Jawaher Albaqami Najla Amin T.Al Kashgry Ahmed Noureldeen department of Biotechnology College of ScienceTaif UniversityTaif21944Saudi Arabia department of Biology College of ScienceTaif UniversityTaif21944Saudi Arabia department of Clinical Laboratory Sciences College of Applied Medical SciencesKing Saud Bin Abdulaziz University for Health SciencesJeddah21423Saudi Arabia department of Biomedical Research King Abdullah International Medical Research CenterJeddah21423Saudi Arabia Biology department Faculty of Science and ArtsAl-Baha UniversityAl-Mikhwah65731Saudi Arebia Faculty of Medicine Mansoura UniversityMansoura35516Egypt department of Chemistry Collage of ScienceTaif UniversityTaif21944Saudi Arabia

The interest in using the datura stramonium plant is due to its natural products,which are used in many pharmaceutical *** objective of the current study was to assess the therapeutic and cytotoxic effects of the *** plant on two types of human cancer cell models(MCF7 and HT29)in vitro.A soxhlet apparatus was used to obtain methanolic extract from dried plant *** recovered crude,after the solvent had evaporated,was then dispersed at varied concentrations of extract 100,50,20,and 0.0µg/mL and tested to see how the cells ***,the cancer-testis antigen(CTA)gene transcription in the two cell types exposed to the plant extract was examined using a semi-quantitative real-time polymerase chain *** chromatography–mass spectrometry(GC-MS)results produced the significant main metabolites Nonanoic acid,Tropine N-Oxide,3,6-ditigloyloxy-7-hydroxytropane,Hexadecanoic acid,2-Pentadecanone,6,10,14-trimethyl-,Carvenone,methyl ester,Phytol,Aposcopolamine,Hyoscyamine,4,8,12,16-Tetramethylheptadecan-4-olide,Scopolamine,Alpha.-Tocospiro A,1,2-Cycloheptanedione,3,3,7,7-tetramethyl-,dihydrazone,Campesterol,Stigmasterol,Gamma-Sitosterol and dl-.alpha.-*** results showed that the two types of cell lines impacted by *** extract,through untreated type 1 cells(MCF7)gave a highly significant transcription according to all applicable *** implemented analyses cleared the strong genetic impacts of datura extract on cancer cells’***2LY and C2orf63 transcript accumulation were also significantly elevated when exposed to plant extract at a level of 50µg/mL in cell line type 2(HT29),but TGIF2LY and P53 had the lowest relative expression at a level of 100µg/mL when treated the same cell line type.

关键词： datura stramonium secondary metabolites GC-MS analysis cytotoxicity breast and colon cancer cell lines MTT assay

Biochemical dissection of STAT3 signaling in amyotrophic lateral sclerosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第11期20卷 3229-3230页

作者： Savina Apolloni Nadia d’Ambrosi department of Biology University of Rome Tor VergataRomeItaly

Amyotrophic lateral sclerosis(ALS)is a progressive neurodegenerative disease characterized by the loss of upper and lower motor neurons,clinically marked by muscle atrophy and *** the clinical course is highly variable,the average time from the onset of symptoms to the need for respiratory support or death is 3-5 *** is the most prevalent motor neuron disease in adults,occurring at a rate of 2 per 100,000 individuals and affecting 5.4 per 100,000 individuals overall.

关键词： STAT3 dissection sclerosis

From Imperfection to Perfection: Advanced 3d Facial Reconstruction Using MICA Models and Self-Supervision Learning

在线全文

学校读者我要写书评

暂无评论

Computer Modeling in Engineering & Sciences 2025年第2期142卷 1459-1479页

作者： Thinh d.Le duong Q.Nguyen Phuong d.Nguyen H.Nguyen-Xuan CIRTECH Institute HUTECH UniversityHo Chi Minh City72308Viet Nam

Research on reconstructing imperfect faces is a challenging *** this study,we explore a data-driven approach using a pre-trained MICA(MetrIC fAce)model combined with 3d printing to address this *** propose a training strategy that utilizes the pre-trained MICA model and self-supervised learning techniques to improve accuracy and reduce the time needed for 3d facial structure *** results demonstrate high accuracy,evaluated by the geometric loss function and various statistical *** showcase the effectiveness of the approach,we used 3d printing to create a model that covers facial *** findings indicate that our method produces a model that fits well and achieves comprehensive 3d facial *** technique has the potential to aid doctors in treating patients with facial injuries.

关键词： 3d face reconstruction self-supervised learning face defect 3d printed prototypes

Anti-amyloid antibodies in Alzheimer’s disease: what did clinical trials teach us?

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第4期20卷 1092-1093页

作者： danko Jeremic Lydia Jiménez-díaz Juan d.Navarro-López NeuroPhysiology&Behavior Lab University of Castilla-La ManchaBiomedical Research Center(CRIB)School of Medicine of Ciudad RealCiudad RealSpain

Although many causes of Alzheimer’s disease(Ad)may exist,both the original amyloid cascade and tau hypotheses posit that abnormal misfolding and accumulation of amyloid-β(Aβ)and tau protein is the central event causing the ***,that conclusion could be only partly true,and there is conflicting evidence about the role of both proteins in Ad,being able to precede and influence one *** researchers argue that these proteins are mere executors rather than primary causes of ***,there have been continuing refinements of both hypotheses,with alternative explanations proposed.Aβand tau proteins may be independently involved in specific neurotoxic pathways;yet there may be other crucial processes going on in early ***,accumulating evidence suggests that Aβand tau act synergistically,rather than additively in disease onset(Jeremic et al.,2021,2023a).

关键词： amyloid Alzheimer additive

Remaking a connection:molecular players involved in post-injury synapse formation

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第6期20卷 1719-1720页

作者： diogo Tomé Ramiro d.Almeida iBiMEd-Institute of Biomedicine Department of Medical SciencesUniversity of AveiroAveiroPortugal CNC-Center for Neuroscience and Cell Biology University of CoimbraCoimbraPortugal

Functional recovery from central nervous system(CNS)trauma depends not only on axon regeneration or compensatory sprouting of uninjured fibers but also on the ability of newly grown axons to establish functional synapses with appropriate *** several studies have successfully promoted long-distance axonal regeneration in distinct CNS injury models,none of them have resulted in a viable therapeutic approach for patient recovery.A possible reason may be the lack of new synaptogenesis for reestablishing the circuitry lost after ***,we discuss how our understanding of the mechanisms that instruct synapse formation in the injured nervous system may contribute to the design of new strategies to promote functional restoration in traumatic CNS disorders.

关键词： viable injury instru