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Microbe/Bug-Busters Visit the Interactive House: An Itinerant Scenographic Device for Health Education

Open Journal of Animal Sciences 2021年第2期11卷 333-353页

作者： Ana Márcia Suarez-Fontes Juliana Almeida-silva sarah cristina dos santos silva Letícia Sant’Anna de Souza Daniele Brum de Souza Conceição Suarez Fontes de Araújo Lima Marcos André Vannier-santos Laboratório de Inovações em Terapia Ensino e Bioprodutos (LITEB) Instituto Oswaldo Cruz (IOC)—Funda&#231&#227o Oswaldo Cruz (Fiocruz) Rio de Janeiro Brazil

The term Ecology is derived from οἶκος (Greek “house” or environment), but our habitations usually overlooked in ecology or environmental studies. The expression “at home” usually means safety and comfort, but at home we are under risk of innumerous parasitic/microbial infections and contaminations/ envenomation. During the COVID-19 pandemic we were forced to stay at home, but the virus and other pathogens were also home-delivered. Education for health is highly effective in health promotion, particularly in poor areas. Thus, prophylactic interventions approaching household environment are required. The present activity aims community empowerment and engagement in controlling parasitic diseases and other infections such as Chagas disease, leishmaniasis, malaria, arboviruses etc. Inspired on the use of a house maket by Dr. Virgínia Schall to demonstrate Aedes mosquitoes breeding sites. We also employ house makets displaying pathogen vectors breeding/hiding sites. Although some makets can be opened, revealing intradomiciliary milieu, we intended to offer the public a literally “insider” view of this largely overlooked scenery. The “Interactive House”, also known as “House with no viruses and other bugs” is an educative, interactive, ludic device elaborated on an inflatable igloo, with furniture and utensils crafted using reused/recycled or low-cost materials. Live mosquito larvae were placed at peridomicile in water-accumulating plant pot saucer, leaves-clogged gutter, used tire, dog water bowl as well as within the domicile, in the shower dra

关键词： Science Communication Health Education One Health Environmental Health Parasitic Diseases

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Inherited Fanconi syndrome

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World Journal of Pediatrics 2023年第7期19卷 619-634页

作者： Anna Luiza Braga Albuquerque Rafael dos santos Borges Ana Flavia Conegundes Erika Emmylaine dos santos Frederico Moreira Man Fu Clara Tavares Araujo Pedro Alves Soares Vaz de Castrol Ana cristina Simoes e silva Interdisciplinary Laboratory of Medical Investigation Unit of Pediatric NephrologyFaculty of MedicineFederal University of Minas Gerais(UFMG)Belo HorizonteBrazil Department of Pediatrics Faculty of MedicineUFMGAlfredo Balena Avenue1902Nd FloorRoom#281Belo HorizonteMG 30130-100Brazil

Background Fanconi-Debré-de Toni syndrome(also known as Fanconi renotubular syndrome,or FRST)profoundly increased the understanding of the functions of the proximal convoluted tubule(PCT)and provided important insights into the pathophysiology of several kidney diseases and drug *** sources We searched Pubmed and Scopus databases to find relevant articles about *** review article focuses on the physiology of the PCT,as well as on the physiopathology of FRST in children,its diagnosis,and *** FRST encompasses a wide variety of inherited and acquired PCT alterations that lead to impairment of PCT *** children,FRST often presents as a secondary feature of systemic disorders that impair energy supply,such as Lowe's syndrome,Dent's disease,cystinosis,hereditary fructose intolerance,galactosemia,tyrosinemia,Alport syndrome,and Wilson's *** rare,congenital causes of FRST greatly impact the morbidity and mortality of patients and impose diagnostic ***,its treatment is diverse and considers the ability of the clinician to identify the correct etiology of the *** The early diagnosis and treatment of pediatric patients with FRST improve the prognosis and the quality of life.

关键词： Cystinosis Fanconi syndrome Fanconi renotubular syndrome Fanconi-Debre-de Toni syndrome Proximal tubule Renal tubular transport-Rickets

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Chemical and microbiological characterization of tinctures and microcapsules loaded with Brazilian red propolis extract

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Journal of Pharmaceutical Analysis 2017年第5期7卷 280-287页

作者： Erika Tayse da Cruz Almeida Maria cristina Delgado da silva José Marcos dos santos Oliveira Regianne Umeko Kamiya Rodolfo Elleson dos santos Arruda Danilo Abreu Vieira Valdemir da Costa silva Pierre Barnabé Escodro Irinaldo Diniz Basílio-Júnior Ticiano Gomes do Nascimento Laboratório de Controle de Qualidade e Análise de Fármacos Medicamentos e AlimentosCurso de FarmáciaEscola de Enfermagem e FarmáciaUniversidade Federal de AlagoasCampus A.C.SimõesBR 104 Norte-Km 97MaceióAL.CEP:57072-970Brazil Laboratório de Controle de Qualidade Microbiológico de Alimentos Faculdade de NutriçãoUniversidade Federal de AlagoasCampus A.C.SimõesBR 104 Norte/Km 97MaceióAL.CEP:57072-970Brazil

The aim of this study was to characterize tinctures and microcapsules loaded with an ethanol extract of red propolis through chemical, physicochemical and microbiological assays in order to establish quality control tools for nutraceutical preparations of red propolis. The markers(isoflavonoids, chalcones, pterocarpans,flavones, phenolic acids, terpenes and guttiferones) present in the tinctures A and B were identified and confirmed using LC/ESI/FTMS/Orbitrap. Four compositions(A, B, C and D) were prepared to contain B tincture of the red propolis with some pharmaceutical excipients and submitted to two drying processes, i. ***-drying and freeze-drying to obtain microcapsules loaded with the red propolis extract. The tinctures and microcapsules of the red propolis were submitted to the total flavonoid content and antioxidant activity *** antibacterial activity and minimum inhibitory concentration(MIC) were tested using Staphylococcus aureus ATCC 25293 and Pseudomonas aeruginosa ATCC 27853 strains. The tinctures and microcapsules presented high flavonoid quantities from 20.50 to 40.79 mg/100 mg of the microcapsules. The antioxidant activity and IC50 were determined for the tinctures A and B(IC50: 6.95 μg/m L and 7.48 μg/m L), the spray-dried microcapsules(IC50: 8.89–15.63 μg/m L) and the freeze-dried microcapsules(IC50: 11.83–23.36 μg/m L). The tinctures and microcapsules were proved to be bioactive against gram-positive and gram-negative bacteria with inhibition halos superior to 10 mm at concentration of 200 μg/m L and MIC values of 135.87–271.74 μg/m L using gram-positive strain and 271.74–543.48 μg/m L using gram-negative strain. The tinctures and microcapsules of the red propolis have a potential application for nutraceutical products.

关键词： Red propolis Tinctures Microcapsules Isoflavonoids LC/ESI/FTMS/Orbitrap Antioxidant activity Antibacterial activity

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Bartter's syndrome:clinical findings,genetic causes and therapeutic approach

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World Journal of Pediatrics 2021年第1期17卷 31-39页

作者： Flavia cristina Carvalho Mrad Sílvia Bouissou Morais Soares Luiz Alberto Wanderley de Menezes silva Pedro Versiani dos Anjos Menezes Ana cristina Simoes-e-silva Interdisciplinary Laboratory of Medical Investigation Faculty of MedicineFederal University of Minas Gerais(UFMG)Av.Alfredo Balena190Room#281Belo HorizonteMG 30130-100Brazil Pediatric Nephrology Unit Faculty of MedicineUFMGBelo HorizonteBrazil

Backgound Bartter's syndrome(BS)is a rare group of salt losing tubulopathies due to the impairment of transport mecha-nisms at the thick ascending limb of the Henle's *** sources Literature reviews and original research articles were collected from database,including PubMed and *** According to the time of onset and symptoms,BS can be classified into antenatal and classic *** studies have identified different subtypes of *** types Ⅰ,Ⅱ and Ⅲ are caused by mutations on genes encoding the luminal Na^(+)-K^(+)-2Cl^(-) co-transporter,the luminal K+ channel ROMK,and the basolateral chloride channel ClC-Kb(CLCNKB),***-of-function mutations of Barttin CLCNK type accessory beta subunit cause BS type Ⅳ*** mutations of CLCNKB and CLCNKA cause BS type Ⅳ*** type Ⅴ consists in a novel transient form characterized by antenatal presentation due to mutations in the MAGE family member *** gain-of-function mutations of the extracellular calcium sensing receptor gene can result in an autosomal dominant condition of *** clinical and biochemical alterations in BS include polyuria,dehydration,hypokalemia,hypochloremic metabolic alka-losis,hyperreninemia,high levels of prostaglandins,normal or low blood pressure,hypercalciuria and failure to *** focuses mainly at correcting dehydration and electrolyte disturbances and in measures to reduce polyuria,including the use of nonsteroidal anti-inflammatory medications to control excessive renal prostaglandin E2 *** Early diagnosis and treatment of BS may prevent long-term consequences such as growth failure,nephrocal-cinosis and end-stage renal disease.

关键词： Bartter's syndrome Hypercalciuria Hypokalemia Metabolic alkalosis Nephrocalcinosis

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Helicobacter pylori in dental plaque and stomach of patients from Northern Brazil

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World Journal of Gastroenterology 2010年第24期16卷 3033-3039页

作者： Mnica Baraúna Assumpo Luisa Caricio Martins Hivana Patricia Melo Barbosa Katarine Antonia dos santos Barile Sintia silva de Almeida Paulo Pimentel Assumpo Tereza cristina de Oliveira Corvelo Endoscopy Department Joo de Barros Barreto University Hospital Immunopathology Laboratory Nucleus of Tropical Medicine Federal University of Pará Immunogenetics Laboratory Federal University of Pará Paulo Pimentel Assumpo Surgery Department Joo de Bar-ros Barreto University Hospital

AIM: To establish whether virulence factor genes vacA and cagA are present in Helicobacter pylori (H. pylori) retrieved from gastric mucosa and dental plaque in pa-tients with dyspepsia. METHODS: Cumulative dental plaque specimens and gastric biopsies were submitted to histological exami-nation, rapid urease test and polymerase chain reac-tion (PCR) assays to detect the presence of cagA and vacA ***: Detection of H. pylori from dental plaque and gastric biopsy samples was greater by PCR com-pared to histological examination and the rapid ure-ase test. DNA from H. pylori was detected in 96% of gastric mucosa samples and in 72% of dental plaque samples. Sixty-three (89%) of 71 dental plaque sam-ples that were H. pylori-positive also exhibited identical vacA and cagA genotypes in gastric mucosa. The most common genotype was vacAs1bm1 and cagA positive, either in dental plaque or gastric mucosa. These viru-lent H. pylori isolates were involved in the severity of clinical ***: These pathogenic strains were found simultaneously in dental plaque and gastric mucosa, which suggests that gastric infection is correlated with the presence of H. pylori in the mouth.

关键词： Helicobacter pylori Gastric mucosa Dental plaque cagA vacA

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Relationship between angiotensin-(1-7) and angiotensin Ⅱ correlates with hemodynamic changes in human liver cirrhosis

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World Journal of Gastroenterology 2009年第20期15卷 2512-2519页

作者： Walkíria Wingester Vilas-Boas Antnio Ribeiro-Oliveira Jr Regina Maria Pereira Renata da Cunha Ribeiro Jerusa Almeida Ana Paula Nadu Ana cristina Simoes e silva Robson Augusto Souza dos santos Laboratory of Hypertension Department of Physiology Biological Sciences institute Federal University of Minas Gerais Av. Antonio Carlos 6627 Belo Horizonte MG 31270-901 Brazil Laboratory of Endocrinology Department of Medicine Faculty of Medicine Federal University of Minas Gerais Av. Alfredo Balena 190 Belo Horizonte MG 30130-100 Brazil Hospitalar Foundation of the State of Minas Gerais FHEMIG Belo Horizonte MG Brazil Department of Pediatrics Faculty of Medicine Federal University of Minas Gerais Av.Alfredo Balena 190 Belo Horizonte MG 30130-100 Brazil

AIM： To measure circulating angiotensins at different stages of human cirrhosis and to further evaluate a possible relationship between renin angiotensin system （RAS） components and hemodynamic changes. METHODS： Patients were allocated into 4 groups： mild-to-moderate liver disease （MLD）, advanced liver disease （ALD）, patients undergoing liver transplantation, and healthy controls. Blood was collected to determine plasma renin activity （PRA）, angiotensin （Ang） Ⅰ, Ang Ⅱ, and Ang-（1-7） levels using radioimmunoassays. During liver transplantation, hemodynamic parameters were determined and blood was simultaneously obtained from the portal vein and radial artery in order to measure RAS components. RESULTS： PRA and angiotensins were elevated in ALD when compared to MLD and controls （P 〈 0.05）. In contrast, Ang Ⅱ was significantly reduced in MLD. Ang-（1-7）/Ang Ⅱ ratios were increased in MLD when compared to controls and ALD. During transplantation, Ang Ⅱ levels were lower and Ang-（1-7）/Ang Ⅱ ratios were higher in the splanchnic circulation than in the peripheral circulation （0.52 ± 0.08 vs 0.38 ±0.04, P 〈 0.02）, whereas the peripheral circulating Ang Ⅱ/Ang Ⅰ ratio was elevated in comparison to splanchnic levels （0.18 ±0.02 vs 0.13 ±0.02, P 〈 0.04）. Ang-（1-7）/ Ang Ⅱ ratios positively correlated with cardiac output （r = 0.66） and negatively correlated with systemic vascular resistance （r = -0.70）. CONCLUSION： Our findings suggest that the relationship between Ang-（1-7） and Ang Ⅱ may play a role in the hemodynamic changes of human cirrhosis.

关键词： Renin-angiotensin system Liver cirrhosis Angiotensin-(1-7) Angiotensin Ⅱ Splanchnic circulation Angiotensin converting enzyme 2

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Multiple hepatic infarctions secondary to diabetic ketoacidosis:A case report

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World Journal of Hepatology 2022年第11期14卷 1977-1984页

作者： Vitoria Mikaelly da silva Gomes Gustavo de Sousa Arantes Ferreira Luise cristina Torres Rubim de Barros Barbara Moreira Ribeiro Trindade dos santos Lorenna Paulinelli Bahia Vieira Department of General Surgery Hospital das Clinicas da Universidade Federal de Minas GeraisBelo Horizonte 30130100Minas GeraisBrazil Instituto de Cardiologia do Distrito Federal Brasilia 70673900Distrito FederalBrazil Department of General Surgery Hospital Metropolitano Doutor Celio de CastroBelo Horizonte 30620090Minas GeraisBrazil

BACKGROUND Hepatic infarctions(HI)are ischemic events of the liver in which a disruption in the blood flow to the hepatocytes leads to focal ischemia and *** HI are due to occlusive events in the liver’s blood vessels,but non-occlusive HI may *** are associated with disruption of microvasculature,such as in diabetic *** HI usually presents as peripheral lesions with clear borders,irregular nodular lesions may occur,indistinguishable from liver neoplasms and presenting a diagnostic *** SUMMARY We report a case of multiple extensive HI in a patient with poorly controlled diabetes mellitus,who first presented to the emergency room with diabetic *** then developed jaundice,thrombocytopenia,and a marked elevation of serum *** ultrasound of the liver showed the presence of multiple irregular *** investigation with a computerized tomography scan confirmed the presence of multiple hypoattenuating nodules with irregular borders and heterogeneous *** lesions were considered highly suggestive of a primary neoplasm of the *** the patient was clinically stable,his bilirubin levels remained persistently elevated,and he underwent an ultrasound-guided percutaneous biopsy of the largest *** results revealed extensive ischemic necrosis of hepatocytes,with no signs of associated *** months after the symptoms,the patient showed great improvement in all clinical and laboratory parameters and extensive regression of the lesions on imaging *** This case highlights that diabetic ketoacidosis can cause non-occlusive HI,possibly presenting as nodular lesions indistinguishable from neoplasms.

关键词： Hepatic infarction Non-occlusive infarcts Diabetic ketoacidosis Pseudotumor of the liver Liver infarcts Case report

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Non-Polar Chemical Constituents of Atemoya and Evaluation of the Cytotoxic and Antimicrobial Activity

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Phyton-International Journal of Experimental Botany 2021年第3期90卷 921-931页

作者： Suzana Vieira Rabêlo Fernanda Granja da silva Oliveira Michele Miriam Calixto de Lira Livia Macedo Dutra Adilson Sartoratto Marta cristina Teixeira Duarte Maria Claudia dos santos Luciano Maria Francilene Souza silva Cláudia doÓPessoa Manoel Odorico de Moraes Filho Emmanoel Vilaça Costa Jackson Roberto Guedes da silva Almeida Post-Graduate Program in Biotechnology(RENORBIO) Federal Rural University of PernambucoRecifePernambuco52171-900Brazil Post-Graduate Program in Biotechnology State University of Feira de SantanaFeira de SantanaBahia44036-900Brazil Center for Studies and Research of Medicinal Plants(NEPLAME) Federal University of San Francisco ValleyPetrolinaPernambuco56304-205Brazil Multidisciplinary Center of Chemical Biological and Agricultural Research(CPQBA)Campinas State UniversityCampinasSão Paulo13083-970Brazil National Laboratory of Experimental Oncology(LabNOE) Federal University of CearáFortalezaCeará60020-181Brazil Departament of Chemistry Federal University of AmazonasManausAmazonas69067-005Brazil

This study aimed to identify the chemical composition of essential oil from fruits(EOAF)and the hexanic crude extract from aerial parts(At-Hex)of atemoya(Annona cherimola x Annona squamosa),a hybrid belonging to the Annonaceae *** and antimicrobial activity was also *** was obtained by hydrodistillation using a Clevenger apparatus,and their composition was analyzed by gas chromatography-mass spectrometry(GC-MS)*** was tested against human tumor cell lines HCT-116(colon carcinoma),SF-295(glioblastoma),OVCAR-8(ovarian carcinoma)and HL60(leukemia)using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT)assay,while antimicrobial activity was conducted by bioauthography method against eleven microorganisms ***-four compounds were identified in the EOAF and twenty-nine in *** monoterpenes linalool(25.70%),α-pinene(10.38%),β-pinene(9.12%),transocimene(7.43%),and the sesquiterpene bicyclogermacrene(12.58%)were the major constituents of EOAF,whereas the sesquiterpene spathulenol(13.91%)was the main compound of ***-Hex showed a high cytotoxicity against SF-295(glioblastoma).These findings show an important chemotaxonomic contribution for the Annonaceae family,mainly for the Annona *** proved to be a promising source of substances with potential cytotoxic activity.

关键词： Annona GC-MS volatile compounds terpenoids biologic activities

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Dietary approach in the treatment of nonalcoholic fatty liver disease

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World Journal of Hepatology 2015年第24期7卷 2522-2534页

作者： Silvia Marinho Ferolla Luciana Costa silva Maria de Lourdes Abreu Ferrari Aloísio Sales da Cunha Flaviano dos santos Martins Cláudia Alves Couto Teresa cristina Abreu Ferrari Departamento de Clínica Médica Centro de Pós-GraduacaoFaculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Departamento de Propedêutica Complementar Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Departamento de Microbiologia Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo Horizonte 31270-901Minas GeraisBrazil

Nonalcoholic fatty liver disease(NAFLD) has been identified as one of the most prevalent chronic liver disease in adults and children populations. NAFLD is usually associated with the metabolic syndrome(MS), which is chiefly related to insulin resistance and its consequences. Insulin resistance has a crucial role in the pathogenesis of hepatic steatosis and potentially nonalcoholic steatohepatitis(NASH). Because of the contemporary epidemics of MS and obesity, the burden of NAFLD is also expected to rise. Unhealthy diets, such as the so-called western diet, are enriched in fructose, trans-fatty acids and saturated fat and seem to be associated with the development of NAFLD. In human studies, certain dietary sugars, particularly fructose, are used as a substrate for lipogenesis leading to hepatic fatty infiltration, inflammation, and possibly fibrosis. Other investigations have shown that fat consumption especially cholesterol and trans/saturated fatty acids are also steatogenic and seem to increase visceral adiposity. The identification of specific dietary components that favor the development of NASH could be important for the management of this disorder. This review focuses on the effects of different dietary approaches to prevent and treat NAFLD emphasizing the macronutrients and energy composition.

关键词： Fatty liver Dietary carbohydrates Dietary fats Die

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Renin-angiotensin system in the pathogenesis of liver fibrosis

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World Journal of Gastroenterology 2009年第21期15卷 2579-2586页

作者： Regina Maria Pereira Robson Augusto Souza dos santos Filipi Leles da Costa Dias Mauro Martins Teixeira Ana cristina Simoes e silva Department of Pediatrics Faculty of MedicineFederal University of Minas Gerais Laboratory of Hypertension Department of Physiology and BiophysicsInstitute of Biological SciencesFederal University of Minas Gerais Laboratory of Immunopharmacology Department of Biochemistry and ImmunologyInstitute of Biological SciencesFederal University of Minas Gerais

Hepatic fibrosis is considered a common response to many chronic hepatic injuries. It is a multifunctional process that involves several cell types, cytokines, chemokines and growth factors leading to a disruption of homeostatic mechanisms that maintain the liver ecosystem. In spite of many studies regarding the development of fibrosis, the understanding of the pathogenesis remains obscure. The hepatic tissue remodeling process is highly complex, resulting from the balance between collagen degradation and synthesis. Among the many mediators that take part in this process, the components of the Renin angiotensin system （RAS） have progressively assumed an important role. Angiotensin （Ang） II acts as a profibrotic mediator and Ang-（1-7）, the newly recognized RAS component, appears to exert a counter-regulatory role in liver tissue. We briefly review the liver fibrosis process and current aspects of the RAS. This review also aims to discuss some experimental evidence regarding the participation of RAS mediators in the pathogenesis of liver fibrosis, focusing on the putative role of the ACE2-Ang-（1-7）- Mas receptor axis.

关键词： Hepatic fibrosis Renin angiotensin system Angiotensin II Angiotensin-(1-7) Receptor Mas Angiotensin converting enzyme 2

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