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Agrobacterium Tumefaciens Based Transformation of Pelargonium x Hortrum cv. ‘Samba’ with Anti-1-aminocyclopropane-1-carboxylate Synthase cDNA to Regulate Ethylene Biosynthesis

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Engineering（科研） 2012年第10期4卷 4-7页

作者： Rajinder S. Ranu Jianguo Fan sarada krishnan Pradeep Agarwal. Amitva Mitra Colorado Sate University Laboratory of Plant Molecular Biology/Biotechnology Fort Collins CO USA 80523. Department of Plant Pathology The University of Nebraska Lincolin NE USA 68583-0722

Phytohormone, ethylene plays an important role in plant growth and development including fruit ripening and flower senescence. The synthesis of 1-aminocylo-propane-1- carboxylate (ACC), the immediate precursor of ethylene, from S-adenosyl-methionine is catalyzed by ACC synthase;and which is also a rate limiting step in the ethylene biosynthetic pathway. Therefore, it plays a key role in ethylene biosynthesis and the genes that code for ACC synthase are of special interest. Moreover, in zonal geraniums, ethylene bursts released from cuttings can have profound impact on the viability of explants for plant propagation. Biotechnological approach involving genetic modification that may reduce ethylene levels has potential for increasing the shelf-life of cuttings for plant propagation. These considerations have led us to clone several cDNA of ACC synthase genes from Pelargonium x hortorum cv. ?‘Sincerity’. To transform geranium cells with Agrobacterium tumefaciens an in vitro regeneration? system was developed using very young petiole explants. An Antisense construct of ACC synthase cDNA (PHSacc41) ligated into binary vector pAM696 was introduced into A. tumefaciens EHA 105 cells. Petiole explants were incubated with the Agrobacterium for 15 min and then co-cultivated for several days on MS medium containing 5 mM BAP and ?1 mM IAA in? the dark without the antibiotics. Selection for transformants was carried out in the presence of kanamycin and timentin. Transgenic plantlets generated were examined for inserted gene cassette by PCR and Southern blotting. Recovery of positive transformants that survived selection suggested that it is possible to transform and introduce genes via transformation in hybrid geraniums for genetic modification.

关键词： ACC Synthase Ethylene Biosynthesis Transformation Geranium Regeneration Agrobacterium Tumefaciens

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Chemical composition and anti-inflammatory activity of Boswellia ovalifoliolata essential oils from leaf and bark

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Journal of Forestry Research 2018年第2期29卷 370-378页

作者： Venkatesan Geetha sarada Nallani Chakravarthula Department of Chemistry School of Advanced SciencesVIT University

We isolated the essential oils from Boswellia ovalifoliolata *** and *** using hydrodistillation,identified the obtained compounds using gas chromatography–mass spectrometry(GC–MS) and Fourier transform-infrared spectroscopy(FT-IR),and studied the photophysical and electrochemical properties using UV–visible and fluorescence spectroscopy and cyclic *** the basis of GC–MS spectra,38 compounds were identified in the essential oil from leaves and 26 in the oil from *** bark oil contained 13.44% b-Farnesene(sesquiterpene),10.45% caryophyllene oxide(sesquiterpene) and 6.6% spathulenol,(2 Z,6 E)-(sesquiterpene alcohol); the leaf oil contained 11.1% spathulenol(sesquiterpene alcohol),9.0% caryophyllene oxide(sesquiterpenoids) and 6.3% decyl acetate(ester).FT-IR spectra confirmed the presence of aliphatic aldehydes and ketones,carboxylic acid,alcohols,esters and ethers in both oil ***–visible absorption spectra showed maximum absorbance at 245,290 and 402 nm for bark oil,and 250,285 and 325 nm for *** oil showed strong emission with maximum emission wavelength at 456 nm was higher than that of leaf oil at 414 *** essential oil had significant anti-inflammatory activity,and the reduction potential of the leaf oil was-0.44 and-0.56 e V for bark.

关键词： Essential oil Boswellia ovalifoliolata Gas chromatography–mass spectrometry Fourier transforminfrared spectroscopy Cyclic voltammetry fluorescence spectroscopy

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Stability assessment of landslide-prone road cut rock slopes in Himalayan terrain:A finite element method based approach

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Journal of Rock Mechanics and Geotechnical Engineering 2020年第1期12卷 59-73页

作者： sarada Prasad Pradhan Tariq Siddique Department of Earth Sciences Indian Institute of Technology RoorkeeRoorkee247667India Department of Geology Aligarh Muslim UniversityAligarh202002India

Large-scale slope destabilization could be aggravated due to swift urbanization and ever-rising demands of geoengineering projects such as dams,tunnels,bridges and widening *** Highway-58 connects Delhi to Badrinath in India,which passes through complex geomorphological and geological terrain and often encounters cut slopes susceptible to slope *** the present investigation,a detailed geotechnical appraisal is conducted along the road cut slopes from Rishikesh to Devprayag in the *** vulnerable road cut slopes were demarcated for detailed slope stability analysis using Phase2D finite element modeling *** generalized Hoek-Brown(GHB)criterion was adopted for stability *** of 20 slopes,five slopes(S6,S7,S18,S19 and S20)are unstable with factor of safety(FoS)less than or equal to 1,and thus needs immediate *** FoS values of four slopes(S2,S9,S13 and S17)lie between 1 and 1.3,*** stable,and slopes S1,S3,S4,S5,S8,S10,Sll,S12,S14,S15 and S16 are ***-Coulomb(MC)criterion was also adopted to compare the slope stability analysis with GHB *** FoS calculated from GHB criterion is close to that using MC criterion for lower values of FoS whereas for higher values,the difference is *** the jointed rock in the Himalayan region,the nonlinear GHB criterion gives better results as compared to MC criterion and matches with the prevailing field ***,some suggestions are proposed to strengthen the stability of cut slopes.

关键词： Landslides Numerical modeling Finite element method(FEM) Slope stability

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Assessment of active tectonics from geomorphic indices and morphometric parameters in part of Ganga basin

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Journal of Mountain Science 2019年第8期16卷 1943-1961页

作者： Aditya Kumar ANAND sarada Prasad PRADHAN Department of Earth Sciences Indian Institute of Technology Roorkee

Ganga river basins exposed to active erosional and deformational processes. The recurrence of landslides, floods, and seismic activities makes it more susceptible to deformational activities. The tectonic analysis using geomorphic indices and morphometric parameters will help in determining the hazard-prone area of the river basin. Geomorphic indices and morphometric parameters are calculated to investigate the role of neotectonic activities, as it acts as a controlling factor in the development of landforms in the tectonically active terrains. Neotectonic activities influence the terrain topography, which significantly affects the drainage system and geomorphological setup of the area. In this study, the assessment of active tectonics of study area was determined using Advanced Spaceborne Thermal Emission and Reflection Radiometer(ASTER) Global Digital Elevation Model(GDEM) based on Geomorphic Indices(Stream Length Gradient index, Hypsometric integral, Asymmetry factor, Basin shape, Valley floor width to Valley height ratio, Mountain front sinuosity index) cumulatively with Linear, Areal and Relief morphometric parameters on 27 delineated basins of the study area. The combined classification of Relative Tectonic Activity Index(Iat) and morphometric parameters of 27 basins categorized all the zones into four different classes:Class 1 – Very High(2.21;299 km^2). The basins with tectonic activities have a consistent relationship with structural disturbances, basin geometry, and field studies. The tectonically active zonation of a part of Ganga basin using geomorphic indices and morphometric parameters suggest that it has significant influence of neotectonic activities in a part of Ganga basin.

关键词： Linear parameters Areal parameters Relief parameters Geomorphic Indices Relative Tectonic Activity Index Ganga river basins

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Geochemistry of a paleosol horizon at the base of the Sausar Group,central India： Implications on atmospheric conditions at the Archeane Paleoproterozoic boundary

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Geoscience Frontiers 2016年第5期7卷 759-773页

作者： sarada P.Mohanty Sangitsarita Nanda Department of Applied Geology. Indian School of Mines Dhanbad 826004 India

A paleosol horizon is described from the contact of the Sausar Group（w2400 Ma） and its basement（Tirodi Gneiss; 〉2500 Ma） in Central India. Physical evidence of pedogenesis is marked by the development of stress corrosion cracks, soil peds, corestone weathering and nodular rocks. XRD and SEM-EDX data indicate the presence of siderite, ankerite, uraninite, chlorite, alumino-silicate minerals, ilmenite,rutile and magnetite, in addition to quartz, feldspar and mica. The chemical index of alteration, the plagioclase index of alteration, and the chemical index of weathering show an increasing trend from parent rock to the paleosol and indicate a moderate trend of weathering. The A-CN-K plot indicates loss of feldspars, enrichment in Al2O3 and formation of illite. Different major element ratios indicate baseloss through hydrolysis, clay formation, leaching of some elements, and more precipitation with good surface drainage. The paleosol is depleted in HREE in comparison to the parent rock indicating high fluid-rock interaction during weathering. The paleosol samples show flat Ce and Eu anomalies, low SREE, and high（La/Yb）N, indicative of a reducing environment of formation. Reducing condition can also be inferred from the concentration of elements such as V, Co, Cu, Pb, and Zn in the paleosol profile. Although enriched in Fe and Mg, the overall geochemical patterns of the paleosol indicate oxygen deficient conditions in the atmosphere and development by weathering and leaching processes associated with high precipitation and good surface drainage at the time of development of this paleosol during the Archeane Paleoproterozoic transition.

关键词： Paleosol Anoxic weathering Great oxidation event Paleoproterozoic Sausar Group

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Plastic waste management for sustainable environment:techniques and approaches

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Waste Disposal and Sustainable Energy 2023年第2期5卷 205-222页

作者： Prashant Pandey Manisha Dhiman Ankur Kansal sarada Prasannan Subudhi Uttarakhand Pollution Control Board Gaura Devi Paryavaran BhawanIT ParkSahastradhara RoadDehradunUttarakhand248001India School of Management IMS Unison UniversityMakkawala GreensMussoorie RoadDehradunUttarakhand248001India

Excessive exploitation,negligence,non-degradable nature,and physical and chemical properties of plastic waste have resulted in a massive pollution load into the ***,plastic entres the food chain and can cause serious health issues in aquatic animals and *** present review summarizes currently reported techniques and approaches for the removal of plastic *** techniques,such as adsorption,coagulation,photocatalysis,and microbial degradation,and approaches like reduction,reuse and recycling are potentially in trend and differ from each other in their efficiency and interaction ***,substantial advantages and challenges associated with these techniques and approaches are highlighted to develop an understanding of the selection of possible ways for a sustainable ***,in addition to the reduction of plastic waste from the ecosystem,many alternative opportunities have also been explored to cash plastic *** fields include the synthesis of adsorbents for the removal of pollutants from aqueous and gaseous stream,their utility in clothing,waste to energy and fuel and in construction(road making).Substantial evidence can be observed in the reduction of plastic pollution from various *** addition,it is important to develop an understanding of factors that need to be emphasized while considering alternative approaches and opportunities to cash plastic waste(like adsorbent,clothing,waste to energy and fuel).The thrust of this review is to provide readers with a comprehensive overview of the development status of techniques and approaches to overcome the global issue of plastic pollution and the outlook on the exploitation of this waste as resources.

关键词： Plastic waste Climate change Adsorption Coagulation Photocatalysis Reduction Reuse Recycling

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One-pot synthesis of poly vinyl alcohol(PVA) supported silver nanoparticles and its efficiency in catalytic reduction of methylene blue

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Transactions of Nonferrous Metals Society of China 2016年第10期26卷 2693-2700页

作者： P.SAGITHA K.sarada K.MURALEEDHARAN Department of Chemistry University of Calicut

Stable silver nanoparticles were synthesized using polyvinyl alcohol （PVA） as reducing and capping agent. The method of steric stabilization was adopted for the incorporation of silver nanoparticles in the polymer matrix. The successful incorporation of silver nanoparticles in a PVA matrix was confirmed by UV–Visible spectroscopy, transmission electron microscopy （TEM） and Fourier transform infrared （FT-IR） spectroscopy. The synthesized silver nanoparticles were characterized by a peak at 426 nm in the UV–Vis spectrum. TEM studies showed the formation of spherical shaped silver nanoparticles of 10-13 nm, following the reduction by UV irradiation. Catalytic properties were studied by means of UV-Visible spectroscopic analysis. The synthesized silver nanoparticles exhibited good catalytic properties in the reduction of methylene blue.

关键词： PVA supported silver nanoparticles dye degradation reduction of methylene blue catalytic reduction

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Intervention Effects on the Willingness to Stop Smoking and Social Nicotine Dependence Based on Single-Session Group Therapy with Frequent Use of Role Play Targeting Smokers with Mental Disorders

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Open Journal of Medical Psychology 2018年第4期7卷 111-122页

作者： Youhei Komatsu Youko sarada Faculty of Rehabilitation Sciences Nishikyushu University Kanzaki Japan Graduate School of Humanities Fukuoka University Fukuoka Japan

Objective: We devised a “Smoking-Cessation Motivation Program” as a community welfare service aimed at making short-term improvements in social nicotine dependence and enhancing the willingness to stop smoking among people with mental disorders. Method: The study conducted a controlled trial to empirically examine the effects of the program on the willingness to stop smoking and social nicotine dependence among 26 smokers with mental disorders in the intervention group, who were compared with a control group of 16 individuals. Results: Results demonstrated the program’s effectiveness in reducing social nicotine dependence, and a variance analysis with age as the covariate evidenced a mutual interaction between social nicotine dependence and the willingness to stop smoking, confidence to stop smoking, and resisting the temptation to smoke. Conclusion: Future studies should examine longer-term program results, the impact of program duration, and other factors that influence the program’s effectiveness.

关键词： People with Mental Disorders Smoking-Cessation Intervention Social Nicotine Dependence

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Evaluation of self-healing properties of inhibitor loaded nanoclay-based anticorrosive coatings on magnesium alloy AZ91D

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Journal of Magnesium and Alloys 2018年第3期6卷 299-308页

作者： Swapnil H.Adsul K.R.C.Soma Raju B.V.sarada Shirish H.Sonawane R.Subasri Centre for Sol-Gel Coatings International Advanced Research Centre for Powder Metallurgy and New Materials(ARCI)BalapurHyderabad 500005TelanganaIndia Centre for Solar Energy Materials International Advanced Research Centre for Powder Metallurgy and New Materials(ARCI)BalapurHyderabad 500005TelanganaIndia Department of Chemical Engineering National Institute of TechnologyWarangal 506004TelanganaIndia

This study emphasizes on the evaluation and comparison of the anticorrosive properties of sol-gel coatings with and without inhibitor loaded *** this case,naturally available clay nanotubes(halloysite)were loaded with cationic corrosion inhibitors Ce 3+/Zr 4+.These nanocontainers were dispersed in hybrid organic-inorganic sol-gel matrix *** was applied on magnesium alloy AZ91D using the sols containing modified and unmodified nanocontainers employing the dip coating method and cured at 130℃for 1 h in *** resistance of coated/uncoated substrates were analyzed using electrochemical impedance spectroscopy,potentiodynamic polarization and weight loss measurements after exposure to 3.5 wt%NaCl solution for varying time durations between 24 h to 120 ***-healing ability of coatings was evaluated by micro-Raman spectroscopy after 120 h exposure to 3.5 wt%NaCl *** generated after dispersion of corrosion inhibitor loaded clay in hybrid sol-gel matrix have shown more promising corrosion resistance when compared to just the sol-gel matrix coatings,after prolonged exposure to corrosive environment.

关键词： Self-healing coating Halloysite nanoclay Cationic corrosion inhibitors Magnesium alloy AZ91D Micro-Raman spectroscopy Corrosion protection

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Physiological Studies in Guinea Grass （Panicum Maximum Jacq.） Varieties as Influenced by Shade Levels and Potash

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材料科学与工程（中英文A版） 2014年第3期4卷 129-133页

作者： Muthayain Retnamma Anita sarada Lakshmi Department of Agronomy College of Agriculture Vellayani

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