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基于YOLOv7网络的高分辨率结核杆菌识别方法研究

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网络新媒体技术 2024年第3期13卷 26-35页

作者：刘三雄山西大学物理与电子工程学院太原030006

结核病已经被列为全球十大主要死亡原因之一,针对痰涂片图像背景复杂,结核杆菌目标尺寸小等难题,提出基于痰涂片图像的单阶段YOLOv7结核杆菌检测网络。骨干网络中,分别在不同尺度的高效聚合网络后嵌入CBAM注意力模块,以提取不同尺度的特... 详细信息

结核病已经被列为全球十大主要死亡原因之一,针对痰涂片图像背景复杂,结核杆菌目标尺寸小等难题,提出基于痰涂片图像的单阶段YOLOv7结核杆菌检测网络。骨干网络中,分别在不同尺度的高效聚合网络后嵌入CBAM注意力模块,以提取不同尺度的特征,在颈部网络中引入分离合并操作以改进MPConv结构,减少深层网络因卷积下采样引起的图像特征损失,并在头部网络中引进高斯分布距离来避免因边界框重叠而引起的小目标漏检,得到一种精度高、检测速度快的结核杆菌方法。实验表明,改进模型的均值平均精度达到了87.8%,相比基线网络模型提升5.1%,且优于同类算法,对推进结核杆菌的智能化检测具有重要意义。

关键词：结核杆菌检测 YOLOv7 注意力机制分离合并操作高斯分布

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早熟优质三系不育系朝湘A的选育

杂交水稻 2024年第1期39卷 65-67页

作者：刘三雄闵军黎用朝刘利成胡敏李小湘黄海明湖南省水稻研究所湖南长沙410125 农业农村部长江中下游籼稻遗传育种重点实验室湖南长沙410125

朝湘A是湖南省水稻研究所用新香B与自育材料海2辐杂交F4代与中间材料泸香618B/玉香B的F4株系复交,自交加代至F4代,再与新香A杂交并连续回交选育而成的野败型优质三系不育系。该不育系异交习性较好,米质优,配合力强,所配组合熟期早,于202... 详细信息

朝湘A是湖南省水稻研究所用新香B与自育材料海2辐杂交F4代与中间材料泸香618B/玉香B的F4株系复交,自交加代至F4代,再与新香A杂交并连续回交选育而成的野败型优质三系不育系。该不育系异交习性较好,米质优,配合力强,所配组合熟期早,于2022年3月通过湖南省农作物品种审定委员会技术鉴定。

关键词：杂交水稻不育系朝湘A 优质选育

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钛基改性DSA电极优化工艺研究与杀菌应用

农产品加工 2024年第10期 61-65,71页

作者：刘野盛庆林岳田利丁亮肖三雄西北大学食品科学与工程学院陕西西安710069 陕西西昂科技集团有限公司陕西西安710086 西安市产品质量监督检验研究院陕西西安710065

对比不同烧结温度制备的Ti/RuO_(2),Ti/IrO_(2),Ti/RuO_(2)-IrO_(2)钛基改性DSA电极的扫描电子显微镜、线性扫描伏安和塔菲尔斜率。结果表明,500℃Ti/RuO_(2)电极在E为0.60 V时通过电极的电流最大(2.645 mA/cm^(2)),塔菲尔斜率最小(109.51 mV/dec),电催化性能最好;450℃Ti/IrO_(2)电极在E为0.60 V时通过电极的电流最大(2.889 mA/cm^(2)),塔菲尔斜率最小(28.09 mV/dec),电催化性能最好;500℃Ti/RuO_(2)-IrO_(2)电极在E为0.60 V时通过电极的电流最大(3.346 mA/cm^(2)),塔菲尔斜率最小(159.61 mV/dec),电催化性能最好;IrO_(2)与RuO_(2)的含量比3∶2时,Ti/RuO_(2)-IrO_(2)电极在E为0.60 V时,通过电极的电流最大(1.546 mA/cm^(2)),塔菲尔斜率最小(191.88 mV/dec),电催化性能最好。采用钛基改性DSA电极制备有效氯质量浓度为40,80和120 mg/L的微酸性电解水,对比不同有效氯质量浓度的微酸性电解水对生菜的保鲜杀菌效果,试验证明微酸性电解水对农产品具有保鲜杀菌作用。

关键词： DSA电极烧结温度塔菲尔斜率农产品保鲜杀菌

Genome-Wide Association Study for Milled Grain Appearance Traits Using Multi-Parent Advanced Generation Intercross Population in Rice

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Rice science 2023年第5期30卷 364-368,I0007-I0012页

作者： LI Xiaoxiang liu Jindong GUO Liang WEI Xiucai WANG Yamei PAN Xiaowu DONG Zheng liu Wenqiang liu Licheng MIN Jun liu sanxiong YE Guoyou LI Yongchao Key Laboratory of Indica Rice Genetics and Breeding in the Middle and Lower Reaches of Yangtze River Valley Hunan Rice Research InstituteHunan Academy of Agricultural SciencesChangsha 410125China Crop Science Institute Chinese Academy of Agricultural SciencesBeijing 100081China Agricultural Genomics Institute Chinese Academy of Agricultural SciencesShenzhen 518120China Rice Breeding Innovations Platform International Rice Research InstituteMetro Manila DAPO Box 7777the Philippines

The identification of loci and markers associated with milled grain appearance traits is essential for breeding high-yielding and good-quality rice variety.To detect stable loci for these characteristics,grain length(GL),grain width(GW),grain length/width(GLW),chalkiness degree(CD),chalky-grain rate(CR)and translucency degree(TD)of 378 rice lines were evaluated in three seasons.These lines were derived from a multi-parent advanced generation intercross(MAGIC)population.

关键词： breeding yielding Population

Detection of Quantitative Trait Loci for Yield and Drought Tolerance Traits in Soybean Using a Recombinant Inbred Line Population

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Journal of Integrative Plant Biology 2009年第9期51卷 868-878页

作者： Weijun Du Deyue Yu sanxiong Fu National Center for Soybean Improvement National Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China College of Agronomy Shanxi Agricultural University Taigu 030801 China

To investigate the genetic basis of drought tolerance in soybean （Glycine max L. Merr.） a recombinant inbred population with 184 F2：7：11 lines developed from a cross between Kefengl （drought tolerant） and Nannong1138-2 （drought sensitive） were tested under water-stressed and well-watered conditions in field and greenhouse trials. Traits measured included leaf wilting coefficient, excised leaf water loss and relative water content as indicators of plant water status and seed yield. A total of 40 quantitative trait loci （QTLs） were identified： 17 for leaf water status traits under drought stress and 23 for seed yield under well-watered and drought-stressed conditions in both field and greenhouse trials. Two seed yield QTLs were detected under both well-watered and drought-stressed conditions in the field on molecular linkage group H and Dlb, while two seed yield QTLs on molecular linkage group C2 were found under greenhouse conditions. Several QTLs for traits associated with plant water status were identified in both field and greenhouse trials, including two leaf wilting coefficient QTLs on molecular linkage group A2 and one excised leaf water loss QTL on molecular linkage group H. Phenotypic correlations of traits suggested several QTLs had pleiotropic or location-linked associations. These results will help to elucidate the genetic basis of drought tolerance in soybean, and could be incorporated into a marker-assisted selection breeding program to develop high-yielding soybean cultivars with improved tolerance to drought stress.

关键词： drought tolerance excised leaf water loss leaf wilting coefficient quantitative trait loci relative water content seed yield soybean（Glycine max L, Merr.）,

Mapping QTLs for seed yield and drought susceptibility index in soybean (Glycine max L.) across different environments

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Journal of Genetics and Genomics 2009年第12期36卷 721-731页

作者： Weijun Du Min Wang sanxiong Fu Deyue Yu National Center for Soybean Improvement National Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural University Nanjing 210095 China College of Agronomy Shanxi Agricultural University Taigu 030801 China

Drought stress has long been a major constraint in maintaining yield stability of soybean （Glycine max （L.） Merr.） in rainfed ecosystems. The identification of consistent quantitative trait loci （QTL） involving seed yield per plant （YP） and drought susceptibility index （DSI） in a population across different environments would therefore be important in molecular marker-assisted breeding of soybean cultivars suitable for rainfed regions. The YP of a recombinant line population of 184 F2：7：11 lines from a cross of Kefengl and Nannong1138-2 was studied under water-stressed （WS） and well-watered （WW） conditions in field （F） and greenhouse （G） trials, and DSI for yield was calculated in two trials. Nineteen QTLs associated with YP-WS and YP-WW, and 10 QTLs associated with DSI, were identi- fied. Comparison of these QTL locations with previous findings showed that the majority of these regions control one or more traits re- lated to yield and other agronomic traits. One QTL on molecular linkage group （MLG） K for YP-F, and two QTLs on MLG C2 for YP-G, remained constant across different water regimes. The regions on MLG C2 for YP-WW-F and MLG H for YP-WS-F had a pleiotropic effect on DSI-F, and MLG A1 for YP-WS-G had a pleiotropic effect on DSI-G. The identification of consistent QTLs for YP and DSI across different environments will significantly improve the efficiency of selecting for drought tolerance in soybean.

关键词： drought susceptibility index （DSI） QTL mapping seed yield per plant （YP） soybean

Identification of QTLs and Validation of qCd-2 Associated with Grain Cadmium Concentrations in Rice

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Rice science 2019年第1期26卷 42-49页

作者： liu Wenqiang PAN Xiaowu LI Yongchao DUAN Yonghong MIN Jun liu sanxiong liu Licheng SHENG Xinnian LI Xiaoxiang Hunan Rice Research Institute Hunan Academy of Agricultural Sciences/Key Laboratory of Indica Rice Genetics and Breeding in the Middle and Lower Reaches of Yangtze River Valley Ministry of Agriculture

Cadmium(Cd) is one of heavy metals harmful to human health. As rice is the main staple food in Asia and Cd is easily contaminated in rice, the molecular regulation of Cd accumulation should be explored. In this study, a recombinant inbred population derived from Xiang 743/Katy was grown in Cd-polluted fields and used to map the quantitative trait loci(QTLs) for Cd accumulation in rice grains. We identified seven QTLs distributed on chromosomes 2, 3, 6, 7, 8 and 10. These QTLs displayed phenotypic variances of 58.50% and 40.59% in 2014 and 2015, respectively. Two QTLs, qCd-2 and qCd-7, were identified in both the two years. qCd-2 was detected on the interval of RM250–RM207 on chromosome 2, with an LOD of 2.51 and a phenotypic contribution of 13.75% in 2014, and an LOD of 3.35 and a phenotypic contribution of 14.16% in 2015. qCd-7 co-localized with the cloned qCdT7 on chromosome 7 and may represent the correct candidate. The other five QTLs were detected only in one year. To further confirm the effects of qCd-2, a residual heterozygous line designated as RHL945, with a heterozygous interval of RM263–RM207 on chromosome 2, was selected from the recombinant inbred population and used to develop an F2 population consisting of 155 individual plants. By incorporating further simple sequence repeat markers into the segmental linkage map of the target region, qCd-2 was delimited in the interval of RM5404–RM3774, with an LOD value of 4.38 and a phenotypic contribution of 15.52%. These results reflected the genetic regulation of grain Cd in rice and paved the way for the future cloning of qCd-2.

关键词： cadmium recombinant inbred line quantitative trait locus rice simple sequence repeat

Ethylene response factor BnERF2-like (ERF2.4) from Brassica napus L.enhances submergence tolerance and alleviates oxidative damage caused by submergence in Arabidopsis thaliana

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The Crop Journal 2016年第3期4卷 199-211页

作者： Yanyan Lv sanxiong Fu Song Chen Wei Zhang Cunkou Qi Institute of Industrial Crops Jiangsu Academy of Agricultural Sciences Jiangsu Collaborative Innovation Center for Modern Crop Production

Ethylene response factor proteins play an important role in regulating a variety of stress responses in plants,but their exact functions in submergence stress are not well understood.In this study,we isolated BnE RF2.4 from Brassica napus L.to study its function in submergence tolerance.The expression of the BnE RF2.4 gene in B.napus and the expression of antioxidant enzyme genes in transgenic Arabidopsis were analyzed by quantitative RT-PCR.The expression of BnE RF2.4 was induced by submergence in B.napus and the overexpression of BnE RF2.4 in Arabidopsis increased the level of tolerance to submergence and oxidative stress.A histochemical method detected lower levels of H_2O_2,O^(·-)_2and malondialdehyde(MDA) in transgenic Arabidopsis.Compared to the wild type,transgenic lines also had higher soluble sugar content and higher activity of antioxidant enzymes,which helped to protect plants against the oxidative damage caused by submergence.It was concluded that BnE RF2.4 increased the tolerance of plants to submergence stress and may be involved in regulating soluble sugar content and the antioxidant system in defense against submergence stress.

关键词： Ethylene response factor Submergence Oxidative damage Ectopic expression Arabidopsis Antioxidant enzyme

深入推进新时代安徽公安工作高质量发展的思考——基于对六安市公安局裕安分局的调查分析

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公安研究 2023年第10期 5-11页

作者：钱三雄安徽省政府安徽省公安厅党委

党的二十大报告指出,“高质量发展是全面建设社会主义现代化国家的首要任务”。公安工作是党和国家工作的重要组成部分,公安工作的高质量发展是全面建设社会主义现代化国家的重要内容、应有之义。本文遵循“面上找问题、点上找答案”的... 详细信息

党的二十大报告指出,“高质量发展是全面建设社会主义现代化国家的首要任务”。公安工作是党和国家工作的重要组成部分,公安工作的高质量发展是全面建设社会主义现代化国家的重要内容、应有之义。本文遵循“面上找问题、点上找答案”的思路,以调查研究的方式,客观总结分析蹲点调研点推进公安工作高质量发展的主要做法,深入查找全省公安系统的问题症结,提出对策建议和实践路径。

关键词：调查研究高质量发展公安工作