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Ultra-highly diluted plant extracts of Hydrastis canadensis and Marsdenia condurango induce epigenetic modifications and alter gene expression profiles in HeLa cells in vitro

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Journal of Integrative Medicine 2015年第6期13卷 400-411页

作者： santu kumar saha Sourav Roy Anisur Rahman Khud a-Bukhsh Cytogenetics and Molecular Biology Laboratory Department of ZoologyUniversity of Kalyani Department of Entomology and Institute for Integrative Genome Biology University of California Riverside

OBJECTIVE： Methylation-specific epigenetic process and gene expression profiles of HeLa cells treated with ultra-high dilutions （HDs） of two plant extracts, Hydrastis canadensis （HC-30） and Marsdenia condurango （Condu-30）, diluted 106o times, were analyzed against placebo 30C （PI-30） for alterations in gene profiles linked to epigenetic modifications. METHODS： Separate groups of cells were subjected to treatment of Condu-30, HC-30, and PI- 30 prepared by serial dilutions and succussions. Global microarray data recorded on Affymetrix platform, using 25-mer probes were provided by iLifeDiscoveries, India. Slides were scanned with 3000 7G microarray scanner and raw data sets were extracted from Cel （raw intensity） files. Analyses of global microarray data profile, differential gene expression, fold change and clusters were made using GeneSpring GX12.5 software and standard normalization procedure. Before microarray study, concentration of RNA （ng/IJL）, RIN value and rRNA ratio for all the samples were analysed by Agilant Bioanalyzer 2100. Reverse transcriptase polymerase chain reaction （RT-PCR） and quantitative RT- PCR were done for analyzing SMAD-4 expression. Fluorescence-activated cell sorting study was further made to elucidate fate of cells at divisional stages. Methylation-specific restriction enzyme assay was conducted for ascertaining methylation status of DNA at specific sites. RESULTS： HDs of HC-30 and Condu-30 differentially altered methylation in specific regions of DNA and expression profiles of certain genes linked to carcinogenesis, as compared to PI-30. Two separate cut sites were found in genomic DNA of untreated and placebo-treated HeLa cells when digested with McrBC, compared to a single cut observed in Condu-30-treated genomic DNA. SMAD-4 gene expression validated the expression pattern observed in microarray profile. Methylation-specific restriction enzyme assay elucidated differential epigenetic modifications in drug-treated and control cells. CON

关键词： plant extracts homeopathy reactive oxygen species apoptosis gene expression epigenesis,genetic Smad4 protein

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Machine learning for predicting the outcome of terminal ballistics events

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Defence Technology（防务技术） 2024年第1期31卷 14-26页

作者： Shannon Ryan Neeraj Mohan Sushma Arun kumar AV Julian Berk Tahrima Hashem santu Rana Svetha Venkatesh Applied Artificial Intelligence Institute(A2I2) Deakin University75 Pigdons RdWaurn PondsVIC3216Australia

Machine learning(ML) is well suited for the prediction of high-complexity,high-dimensional problems such as those encountered in terminal *** evaluate the performance of four popular ML-based regression models,extreme gradient boosting(XGBoost),artificial neural network(ANN),support vector regression(SVR),and Gaussian process regression(GP),on two common terminal ballistics’ problems:(a)predicting the V50ballistic limit of monolithic metallic armour impacted by small and medium calibre projectiles and fragments,and(b) predicting the depth to which a projectile will penetrate a target of semi-infinite *** achieve this we utilise two datasets,each consisting of approximately 1000samples,collated from public release *** demonstrate that all four model types provide similarly excellent agreement when interpolating within the training data and diverge when extrapolating outside this *** extrapolation is not advisable for ML-based regression models,for applications such as lethality/survivability analysis,such capability is *** circumvent this,we implement expert knowledge and physics-based models via enforced monotonicity,as a Gaussian prior mean,and through a modified loss *** physics-informed models demonstrate improved performance over both classical physics-based models and the basic ML regression models,providing an ability to accurately fit experimental data when it is available and then revert to the physics-based model when *** resulting models demonstrate high levels of predictive accuracy over a very wide range of projectile types,target materials and thicknesses,and impact conditions significantly more diverse than that achievable from any existing analytical *** with numerical analysis tools such as finite element solvers the ML models run orders of magnitude *** provide some general guidelines throughout for the development,application,and reporting of ML models in terminal ballist

关键词： Machine learning Artificial intelligence Physics-informed machine learning Terminal ballistics Armour

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高度稀释的顺势疗法药物对噬菌体感染的细菌基因水平的作用(英文)

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中西医结合学报 2012年第4期10卷 462-470页

作者： santu kumar saha Sreemanti Das Anisur Rahman Khuda-Bukhsh Cytogenetics and Molecular Biology Laboratory Department of ZoologyUniversity of KalyaniKalyani 741235India

目的:探讨具有抗病毒作用的高度稀释的顺势疗法药物对噬菌体感染的大肠杆菌在基因水平调节噬菌体ΦX174 DNA的作用。方法:本研究之所以选用噬菌体ΦX174是因为其对大肠杆菌的宿主特异性及其在宿主内进行溶菌素基因E的组成性表达。采用... 详细信息

目的:探讨具有抗病毒作用的高度稀释的顺势疗法药物对噬菌体感染的大肠杆菌在基因水平调节噬菌体ΦX174 DNA的作用。方法:本研究之所以选用噬菌体ΦX174是因为其对大肠杆菌的宿主特异性及其在宿主内进行溶菌素基因E的组成性表达。采用顶层琼脂法,计数琼脂板上的斑块数量以衡量不同的顺势疗法药物对噬菌体感染的大肠杆菌的保护作用。被噬菌体感染的大肠杆菌接受不同顺势疗法药物的干预,以高度稀释的乙醇做为安慰剂对照,并加设空白对照组。琼脂板上的斑块数量表明菌群被噬菌体ΦX174感染并溶解的数量。反之,我们在用顺势疗法药物干预前将噬菌体ΦX174混入药物中,再与细菌作用,以确定药物本身对感染细菌的噬菌体ΦX174没有作用。结果:每一种顺势疗法药物干预后的细菌琼脂板上的斑块数量均较安慰剂对照组和空白对照组有显著下降;而混入药物的噬菌体ΦX174感染细菌后,琼脂板上的斑块数量并无明显下降。因为噬菌体ΦX174在细菌内开始其溶菌过程,斑块数量的下降可能是因为溶菌素基因E被抑制或者整个噬菌体ΦX174的DNA被大肠杆菌内的基因产物(抑制酶)所破坏。结论:本研究的结果证实了高度稀释的顺势疗法药物对噬菌体感染的大肠杆菌在基因水平有调节作用。

关键词：细菌噬菌体φX174 大肠杆菌顺势疗法基因表达调控菌斑试验

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顺势疗法药物山金车30C通过上调核苷酸切除修复基因的表达减少紫外线照射后大肠杆菌的DNA损伤(英文)

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中西医结合学报 2012年第3期10卷 337-346页

作者： Sreemanti Das santu kumar saha Arnab De Durba Das Anisur Rahman Khuda-Bukhsh Cytogenetics and Molecular Biology Laboratory Department of ZoologyUniversity of Kalyani

目的:检测高度稀释的顺势疗法药物山金车30C是否能够调节暴露于紫外线照射下的大肠杆菌的核苷酸切除修复基因的表达。方法:大肠杆菌在标准培养基中培养至对数阶段,然后接受亚致死剂量的紫外线照射(25和50 J/m2分别照射22.5和45 s)。接... 详细信息

目的:检测高度稀释的顺势疗法药物山金车30C是否能够调节暴露于紫外线照射下的大肠杆菌的核苷酸切除修复基因的表达。方法:大肠杆菌在标准培养基中培养至对数阶段,然后接受亚致死剂量的紫外线照射(25和50 J/m2分别照射22.5和45 s)。接受不同剂量紫外线照射的大肠杆菌分别与山金车30C及安慰剂30C共同培养,90 min后检测其DNA损伤情况及氧化应激状态。采用多种方法及指标如彗星实验、梯度凝胶电泳、细胞内活性氧生成及测量其他生物活性指标如过氧化物歧化酶、过氧化氢酶及谷胱甘肽衡量DNA损伤情况及细胞氧化应激状态。逆转录聚合酶链反应检测大肠杆菌细胞紫外线损伤修复基因uvrA、B、C(核苷酸切除修复基因)mRNA的表达情况。结果:接受照射后的大肠杆菌出现了DNA损伤及氧化应激反应,表现为细胞内活性氧生成增加及过氧化物歧化酶、过氧化氢酶和谷胱甘肽活性降低。与安慰剂组相比,山金车30C降低了大肠杆菌的DNA损伤及氧化应激反应,表现为细胞内活性氧生成减少及过氧化物歧化酶、过氧化氢酶和谷胱甘肽活性增强。与对照组相比,山金车30C上调了大肠杆菌细胞紫外线损伤修复基因的表达。结论:山金车30C能够通过上调紫外线损伤修复基因的表达修复紫外线引起的大肠杆菌细胞的DNA损伤,并通过减少细胞内活性氧的生成及调节抗氧化酶活性降低细胞的氧化应激反应。

关键词：大肠杆菌紫外线顺势疗法山金车属活性氧 DNA损伤 DNA修复

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