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Induction of IgA and sustained deficiency of cell proliferative response in chronic hepatitis C

World Journal of Gastroenterology 2008年第44期14卷 6844-6852页

作者： Yalena Amador-Caizares Liz Alvarez-Lajonchere Ivis Guerra Ingrid Rodríguez-Alonso Gillian Martínez-Donato Julián Triana Eddy E González-Horta Angel Pérez santiago dueas-carrera Centro de Ingeniería Genética y Biotecnología Ave 31e/ 158 and 190PlayaP.O. Box 6162HavanaCuba

AIM： In the present study, antibody and peripheral blood mononuclear cells （PBMC） proliferative responses against hepatitis C virus （HCV） antigens were evaluated in HCV chronically infected patients. METHODS： Paired serum and PBMC samples were taken six months apart from 34 individuals, either treated or not, and tested by enzyme-linked immunosorbent assay （ELISA） and carboxyfluorescein succinimidyl ester staining. RSULTS： Over 70% of the patients showed specific IgG and IgM against capsid, E1 and NS3, while HVR-1 was recognized by half of the patients. An increase in the levels of the anti-capsid IgM （P = 0.027） and IgG （P = 0.0006） was observed in six-month samples, compared to baseline. Similarly, a significantly higher percent of patients had detectable IgA reactivity to capsid （P = 0.017） and NS3 （P = 0.005） after six months, compared to baseline. Particularly, IgA against structural antigens positively correlated with hepatic damage （P = 0.036）. IgG subclasses evaluation against capsid and NS3 revealed a positive recognition mediated by IgG1 in more than 80% of the individuals. On the contrary, less than 30% of the patients showed a positive proliferative response either of CD4＋ or CD8＋ T cells, being the capsid poorly recognized. CONCLUSION： These results confirm that while the cellular immune response is narrow and weak, a broad and vigorous humoral response occurs in HCV chronic infection. The observed correlation between IgA and hepatic damage may have diagnostic significance, although it warrants further confirmation.

关键词： Hepatitis C Antibody response Lymphoproliferation Core Envelope

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HCV-specific immune responses induced by CIGB-230 in combination with IFN-α plus ribavirin

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World Journal of Gastroenterology 2014年第1期20卷 148-162页

作者： Yalena Amador-Caizares Gillian Martínez-Donato Liz lvarez-Lajonchere Claudia Vasallo Mariacarla Dausá Daylen Aguilar-Noriega Carmen Valenzuela Ivette Raíces Jean Dubuisson Czeslaw Wychowski Zurina Cinza-Estévez Marlén Castellanos Magdalys Núez Anny Armas Yaimé González Ismariley Revé Ivis Guerra ngel Pérez Aguiar santiago dueas-carrera Centro de Ingeniería Genética y Biotecnología Playa 10600HavanaCuba Center for Infection and Immunity of Lille (CIIL) Inserm U1019CNRS UMR8204Institut Pasteur de LilleUniv Lille Nord de FranceF-59000 LilleFrance Instituto de Gastroenterología Vedado 10400HavanaCuba Hospital Clínico Quirúrgico "Hermanos Amejeiras" Havana 10200Cuba Centro de Inmunoensayos Havana 11300Cuba

AIM: To analyze hepatitis C virus (HCV)-specific immune responses in chronically infected patients under triple therapy with interferon-α (IFN-α) plus ribavirin and CIGB-230.

关键词： Clinical trial DNA vaccine Enzyme-linked immunospot Hepatitis C virus Leukopenia

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Influence of Angiotensin II on α₁-Adrenergic Receptors Function in Rat Aorta and Expression in Vascular Smooth Muscle Cells

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Journal of Biosciences and Medicines 2024年第4期12卷 123-134页

作者： Itzell Alejandrina Gallardo-Ortíz Juan Pablo de Jesús Benítez-Garrido santiago C. Sigrist-Flores Juan Javier López-Guerrero Enrique Hong Rafael Villalobos-Molina Unidad de Biomedicina Facultad de Estudios Superiores Iztacala Universidad Nacional Aut&#243noma de M&#233xico Tlalnepantla M&#233xico carrera de Enfermerí a Facultad de Estudios Superiores Iztacala Universidad Nacional Aut&#243noma de M&#233xico Tlalnepantla M&#233xico Departamento de Farmacobiologí a Centro de Investigaci&#243n and de Estudios Avanzados-IPN Ciudad de M&#233xico M&#233xico carrera de Mé dico Cirujano Facultad de Estudios Superiores Iztacala Universidad Nacional Aut&#243noma de M&#233xico Tlalnepantla M&#233xico

Angiotensin II (Ang II) is the main mediator of the Renin-Angiotensin-System acting on AT1 and other AT receptors. It is regarded as a pleiotropic agent that induces many actions, including functioning as a growth factor, and as a contractile hormone, among others. The aim of this work was to examine the impact of Ang II on the expression and function of α1-adrenergic receptors (α1-ARs) in cultured rat aorta, and aorta-derived smooth muscle cells. Isolated Wistar rat aorta was incubated for 24 h in DMEM at 37˚C, then subjected to isometric tension and to the action of added norepinephrine, in concentration-response curves. Ang II was added (1 × 10−5 M), and in some experiments, 5-Methylurapidil (α1A-AR antagonist), AH11110A (α1B-AR antagonist), or BMY-7378 (α1D-AR antagonist), were used to identify the α1-AR involved in the response. Desensitization of the contractile response to norepinephrine was observed due to incubation time, and by the Ang II action. α1D-AR was protected from desensitization by BMY-7378;while RS-100329 and prazosin partially mitigated desensitization. In another set of experiments, isolated aorta-derived smooth muscle cells were exposed to Ang II and α1-ARs proteins were evaluated. α1D-AR increased at 30 and 60 min post Ang II exposure, the α1A-AR diminished from 1 to 4 h, while α1B-AR remained unchanged over 24 h of Ang II exposure. Ang II induced an increase of α1D-AR at short times, and BMY-7378 protected α1D-AR from desensitization.

关键词： Angiotensin II α1D-AR α1-AR Expression Rat aorta Smooth Muscle Cells

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Psychotherapy in anorexia nervosa: What does the absence of evidence mean?

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World Journal of Translational Medicine 2014年第3期3卷 150-157页

作者： Emilio Gutiérrez Olaia carrera Unidad Venres Clínicos Facultad de Psicología Campus Vida15782 Santiago de Compostela Spain Departamento de Psicología Clínica y Psicobiología Facultad de Psicología Universidad de SantiagoCampus Vida 15782 Santiago de Compostela Spain

Psychological treatment in anorexia nervosa(AN) is disheartening. Psychotherapy is the "treatment of choice" for adults though this recommendation is grounded on the absence of good quality clinical studies. This paper seeks to address the question of why improvements in the psychological treatment of AN have been thwarted, and why one of the best treatments available for adult patients is specialist supportive clinical management that has entered the stage through the backdoor of nonspecific supportive treatments originally serving as a placebo treatment assigned in randomized clinical trials to control for non-specific aspects of true psychosocial treatments. The possibility that most of the psychopathological features that characterise the AN symptoms profile could be best understood as the direct consequences of emaciation would enhance the utility of research with animal models for generating new hypothesis to improve AN treatment.

关键词： Psychotherapy Anorexia nervosa Specialist supportive clinical management Non-specific Placebo Efficacy

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Endogenous Norepinephrine Desensitizes α_1D-Adrenoceptors in Cultured Rat Aorta

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Journal of Biosciences and Medicines 2023年第10期11卷 168-180页

作者： Itzell Alejandrina Gallardo-Ortíz Jesús David Gómez-Rodríguez Juan Javier López-Guerrero santiago C. Sigrist-Flores Rafael Villalobos-Molina Unidad de Biomedicina Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México Tlalnepantla México carrera de Enfermería Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México Tlalnepantla México Ciencias de la Salud Unidad Xochimilco Universidad Autónoma Metropolitana Ciudad de México México carrera de Médico Cirujano Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México Tlalnepantla México

Desensitization is a process characterized by the loss of cellular response to an agonist when this is present for a long time. α1D-adrenergic receptor (α1D-AR) desensitization is important since this receptor is involved in the contraction of large caliber arteries, such as the aorta. The aim of this research was to evaluate the desensitization of α1D-AR due to the endogenous release of norepinephrine in cultured rat aorta. Wistar rat aorta was incubated for 2 h or 24 h in DMEM at 37°C, and then subjected to isometric tension and the action of added norepinephrine, in concentration-response curve (CRC). In some experiments, BMY-7378 (α1D-AR antagonist) or 5-methylurapidil (α1A-AR antagonist) was used to identify the α1-AR involved in the response, or BMY-7378 to protect the α1D-AR from desensitization. Results showed that α1D-AR was desensitized when the aorta was incubated for 24 h, since the CRC to exogenous norepinephrine showed lower maximal contraction and the curve was displaced to the right, indicating that the receptor involved in contraction was not the α1D-AR, as compared to the aorta incubated 2 h. The receptor stimulated by norepinephrine at 24 h was neither the α1A-AR, as shown by the lack of displacement of the curve by 5-methylurapidil, but rather it seems that α1B-AR is inducing contraction. When the aorta was incubated with BMY-7378 for 24 h, the α1D-AR antagonist protected the receptor from desensitization. Endogenous norepinephrine desensitizes α1D-AR in the cultured aorta, and the α1D-AR is protected by BMY-7378.

关键词： Desensitization Norepinephrine α1D-Adrenergic Receptor BMY-7378 Rat Aorta

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The body mass index (BMI) as a public health tool to predict metabolic syndrome

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Open Journal of Preventive Medicine 2012年第1期2卷 59-66页

作者： Miguel Murguía-Romero Rafael Jiménez-Flores Rafael Villalobos-Molina María Isabel Mendoza-Ramos Julia Reyes-Reali santiago C. Sigrist-Flores Adolfo René Méndez-Cruz carrera de Médico Cirujano FES Iztacala UNAM Tlalnepantla México

Objective: To analyze the body mass index (BMI) as an indicator of metabolic alterations, including the metabolic syndrome (MetS), at both individual level and public health level. Method: We recruited 3683 undergraduate students (17 - 24 years old) from México City identifying metabolic alterations, including the MetS, and comparing its prevalence by BMI ranges. We applied a sensitivity analysis to define BMI optimal cut-off point values. Results: We found 14.6% of MetS prevalence with a BMI average of 24.2%, and 34.5% of overweight prevalence (BMI ≥ 25). A BMI cut-off point value of 22.5 is suggested as an upper limit of a normal weight condition, only for public health purpose;while at individual level the BMI cut-off point of 25 was corroborated as the upper limit for a normal weight condition. A public health tool to estimate the MetS prevalence based on BMI percentages is proposed, and a study case is presented. Conclusion: BMI fails predicting at individual level both, healthy condition or metabolic alterations, when values are lower than 25. At population level, the BMI is a valuable public health tool to estimate MetS prevalence: based on the prevalence of MetS by BMI ranges of a sample of the population.

关键词： BMI Metabolic Syndrome Obesity Public Health Tool

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Metabolic syndrome occurrence in university students from México City: The binomium HDL/waist circumference is the major prevalence factor

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Open Journal of Preventive Medicine 2012年第2期2卷 177-182页

作者： J. Rafael Jiménez-Flores Miguel Murguía-Romero M. Isabel Mendoza-Ramos santiago Sigrist-Flores Norma Y. Rodríguez-Soriano Lilia I. Ramírez-García Ramiro Jesús-Sandoval M. Araceli álvarez-Gasca Esther Orozco Rafael Villalobos-Molina A. René Méndez-Cruz carrera de Médico Cirujano FES Iztacala UNAM Tlalnepantla México carrera de Psicología FES Iztacala UNAM Tlalnepantla México Posgrado en Ciencias Biológicas FES Iztacala UNAM Tlalnepantla México Unidad de Biomedicina FES Iztacala UNAM Tlalnepantla México Universidad Autónoma de la Ciudad de México Ciudad de México México

Objective: Metabolic Syndrome (MetS) is the leading cause to develop type 2 diabetes worldwide. We examined associations of MetS components early in life, and their use as risk factors of acquiring MetS. Method: We used an international definition of MetS. Subjects were categorized into “Healthy”/“Not Healthy”, altered parameters are low HDL-cholesterol, large waist circumference (WC), hypertriacylglycerolemia, hypertension, and hyperglycemia, in 32 combinations (2^5) with two values (altered/not altered). MetS was identified with three or more altered parameters. Results: A total of 3424 students (ages 17 - 24 years) participated in the survey, and 2475 were “Not Healthy” showing at least 1 parameter altered;from them 49.6% showed low blood HDL either alone or combined, 38.2% had altered waist circumference either alone or combined;while 18.1% showed hypertriacylglycero-lemia either alone or combined. Hypertension and hyperglycemia were the lowest in frequency. Conclusion: We propose that the binomium HDL/ Waist Circumference is the main prevalence factor to develop MetS in the asymptomatic young population, followed by hypertriacylglycerolemia which together define MetS;while hypertension and hyperglycemia seem to occur later in MetS.

关键词： HDL/Waist Circumference Metabolic Syndrome Mexican University Students

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Turbo-taxonomy to assemble a megadiverse lichen genus:seventy new species of Cora(Basidiomycota:Agaricales:Hygrophoraceae),honouring David Leslie Hawksworth’s seventieth birthday

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Fungal Diversity 2017年第3期84卷 139-207页

作者： Robert Lücking Manuela Dal Forno Bibiana Moncada Luis Fernando Coca Leidy Yasmín Vargas-Mendoza André Aptroot Laura Juliana Arias Betty Besal Frank Bungartz Diego Mauricio Cabrera-Amaya Marcela E.S.Cáceres José Luis Chaves Sionara Eliasaro Martha Cecilia Gutiérrez Jesús E.Hernández Marin María de los Ángeles Herrera-Campos María E.Holgado-Rojas Harald Jonitz Martin Kukwa Fabiane Lucheta santiago Madriñán Marcelo Pinto Marcelli Suzana Maria de Azevedo Martins Joel A.Mercado-Díaz Jorge Alberto Molina Eduardo A.Morales Peter R.Nelson Freddy Nugra Francisco Ortega Telma Paredes Ayda Lucía Patiño Rouchi Nadine Peláez-Pulido Rosa Emilia Pérez Pérez Gary B.Perlmutter Eimy Rivas-Plata Javier Robayo Camilo Rodríguez Diego Fernando Simijaca Edier Soto-Medina Adriano Afonso Spielmann Alejandra Suárez-Corredor Jean-Marc Torres Carlos Alberto Vargas Alba Yánez-Ayabaca Gothamie Weerakoon Karina Wilk Marcela Celis Pacheco Mauricio Diazgranados Grischa Brokamp Thomas Borsch Patrick M.Gillevet Masoumeh Sikaroodi James D.Lawrey Botanical Garden and Botanical Museum Berlin KöniginLuise-Straße 6-814195 BerlinGermany Science Education The Field Museum1400 South Lake ShoreChicagoIL 60605USA Department of Botany Smithsonian InstitutionNational Museum of Natural History10th St Constitution Ave NWWashingtonDC 20560USA Department of Environmental Science and Policy George Mason UniversityFairfaxVA 22030-4444USA Licenciatura en Biologı´a Universidad Distrital Francisco Jose´de CaldasCra.4 No.26D-54Torre de LaboratoriosHerbarioBogota´D.C.Colombia Herbario Universidad de Caldas(FAUC) Edificio BicentenarioCalle 65No.26-10ManizalesA.A.275CaldasColombia ABL Herbarium G.v.d.Veenstraat 1073762 XK SoestThe Netherlands Biologı´a Universidad del Quindı´oCarrera 15 Calle 12 NorteArmeniaQuindı´oColombia 120 Chavis Avenue LexingtonVA 24450USA Charles Darwin Foundation Puerto AyoraSanta Cruz IslandGala´pagosEcuador Schedestraße 8 53113 BonnGermany Jardı´n Bota´nico de Bogota´Jose´Celestino Mutis Avenida Calle 63 No.68-95Bogota´D.C.Colombia Departamento de Biocieˆncias Universidade Federal de SergipeAvenida Vereador Olı´mpio Grandes/n.ItabaianaCEP:49.500-000 SergipeBrazil Laboratorio de Hongos Instituto Nacional de Biodiversidad(INBio)Apdo.22-3100Santo Domingo de HerediaCosta Rica Departamento de Botaˆnica Universidade Federal do Parana´Campus Polite´cnicoSetor de Cieˆncias Biolo´gicasAv.Cel.Francisco H.dos Santoss/nJardim das Ame´ricas81531-980 CuritibaPRBrazil Ingenierı´a Ambiental Universidad Distrital Francisco Jose´de CaldasCarrera 5 Este No.15-82Av.CircumvalarBogota´D.C.Colombia Instituto Experimental Jardı´n Bota´nico Dr.Tobı´as Lasser Centro de Investigacio´n y DesarrolloUniversidad Central de VenezuelaAve.Salvador AllendeJardı´n Bota´nico de CaracasApartado#2156Caracas 1010-AVenezuela Departamento de Bota´nica Instituto de Biologı´aUniversidad Nacional Auto´noma de Me´xicoApartado Postal 70-36704510 Ciudad De Me´xicoMexico Facultad de Ciencias Escuela Profesional de Biologı´aUniv

Following a large-scale phylogenetic study of the lichenized genus Cora(Basidiomycota:Agaricales:Hygrophoraceae),we formally describe 70 new species,honouring the seventieth birthday of David Leslie Hawksworth,one of the preeminent figures in mycology and lichenology in the past 50 *** on an updated phylogeny using the ITS fungal barcoding locus,we now recognize 189 taxa in a genus that until recently was considered to represent a single species;including this contribution,92 of these are formally recognized,including five taxa based on historical names or collections that have not been *** of Cora can be recognized by a combination of morphological(size,colour,lobe configuration,surface hairs,hymenophore size and shape),anatomical(thallus thickness,cortex structure,photobiont type,hyphal papillae),and ecogeographical features(substrate,habitat,distribution),and a keytable allowing the identification of all accepted taxa is *** new species are:Cora accipiter Moncada,Madrin˜a´n&Lücking ***.,*** Moncada,Soto-Medina&Lücking ***.,*** Moncada,Dal Forno&Lücking ***.,*** Dal Forno,Chaves&Lücking ***.,*** Moncada,***ı´guez&Lücking ***.,*** Lücking,Moncada&*** ***.,*** Moncada,Ya´nez-Ayabaca&Lücking ***.,*** Moncada,Patin˜o&Lücking ***.,*** Lücking,***&Dal Forno ***.,*** Holgado,Rivas Plata&Perlmutter ***.,*** Dal Forno,Eliasaro&Spielmann ***.,*** Nugra,Dal Forno&Lücking ***.,*** Lücking,Martins&Lucheta ***.,*** Moncada,R.-***´rez&Lücking ***.,*** Moncada,***.&Lücking ***.,*** Moncada,CabreraAmaya&Lücking ***.,*** Moncada,R.-***´rez&***.,*** Lücking,***&Dal Forno ***.,*** Moncada,***´rez-Corredor&Lücking ***.,*** Moncada,***&Lücking ***.,***

关键词： Bolivia Citizen science Chile Mexico Puerto Rico Sri Lanka

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