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EFFECT OF TOURISM ON THE DISTRIBUTION OF LARVAL BLACKFLIES (DIPTERA：SIMULIUM) IN PALNI HILLS OF SOUTH INDIA

水生生物学报 2011年第4期35卷 688-692页

作者： sankarappan anbalagan Sundaram Dinakaran Jeyaraj Pandiarajan Muthukalingan Krishnan Department of Environmental Biotechnology Bharathidasan University Tiruchirappalli-620024 Tamil Nadu India Centre for Research in Aquatic Entomology The Madura College Madurai-625016 Tamil Nadu India

The effect of tourism on the distribution of larval blackflies during three different seasons of Palni hills was *** streams were selected(Kumbakkarai,Moolayar,Kurusedi,Silver cascade Pampar and Guntar) for this study,of which,three streams(Silver cascade Pampar and Guntar) were in peak of Palni hills(Kodaikanal town areas) and easy access to the *** remaining three streams(Kumbakkarai,Moolayar and Kurusedi) were in downhill *** physico-chemical and stream parameters analyses indicated that the most tourist accessible site of Silver cas-cade stream had poor water quality and the predominant of larval blackfly population when compared to other sampling *** highest population of larval blackflies was observed during winter followed by summer and northeast *** the effect of tourism causes pollution to streams,which in turn to promote the population of blackfly larvae and affect the other insect community in streams.

关键词：英文摘要内容介绍编辑工作盘尾丝虫

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A simple and cost-effective protocol for extraction of genomic DNA from ethanol preserved black flies(Simuliidae:Diptera)

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Journal of Coastal Life Medicine 2016年第9期4卷 721-725页

作者： Neelamegam Rameshkumar sankarappan anbalagan Nagarajan Kayalvizhi Rasiravuthanahalli Kaveriyappan Govindarajan Vimalanathan Arun Prasanna Muthukalingan Krishnan Department of Environmental Biotechnology Bharathidasan UniversityTiruchirappalli 620024Tamil NaduIndia Department of Zoology Sethupathy Government Arts CollegeRamanathapuram 623502Tamil NaduIndia Department of Zoology Periyar UniversitySalem 636001Tamil NaduIndia

Objective:To investigate the efficacy of extraction methodology for the proposed DNA from the ethanol preserved black flies(Simuliidae:Diptera).Methods:This study addressed a simple and effective protocol for extraction of DNA from black flies stored in the *** sizes of larval and adult black flies ranged from 1.5 to 2.5 mm and 3 to 7 mm,*** demonstrate the efficacy of the proposed methodology,the DNA was extracted from the ethanol preserved sample of black flies using the commercial *** extracted DNA was further validated in the PCR amplification using internal transcribed spacer-1 rDNA and cytochrome oxidase subunit II rDNA target ***:Interestingly,a minor modification in the proposed methodology yielded a good DNA concentration in comparison with the commercial *** extracted DNA sample using the proposed methodology was successfully validated in the PCR amplification using internal transcribed spacer-1 rDNA and cytochrome oxidase subunit II rDNA ***:The proposed DNA extraction procedure yielded good concentration of DNA from the ethanol preserved black *** added advantage is that the procedure is suitable for a range of insect species preserved in the ethanol obtained from the various field conditions.

关键词： Black flies Ethanol preservation DNA extraction PCR Amplification

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