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Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim Kyeong-Hyeon Eom Dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun Kim Jun-Koo Yi Jae-Jung Ha Ji-Hyun Lee Seong-Beom Lee Woo-Jae Son soo-Young Yum Won-Wu Lee Goo Jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing has been considered as powerful tool in agricultural ***,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising ***,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in *** this point of view,electroporation in embryos has been risen as an *** First,editing efficiency of our electroporation methods were tested for *** of mutation on embryo was confirmed by T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both *** These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively ***,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

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Amine functionalization on thermal and mechanical behaviors of graphite nanofibers-loaded epoxy composites

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Journal of Materials Science & Technology 2023年第20期151卷 80-88页

作者： Seong-Hwang Kim sang-Jin Park Seul-Yi Lee soo-Jin Park Department of Chemistry Inha University100 InharoIncheon 22212the Republic of Korea

With the growing demand for faster and more powerful computing,effective heat dissipation is essential to ensure the longevity,reliability,and high performance of electronic *** the field of modern electronic packaging materials,there is a great need for graphitic material-loaded polymeric composites(GPCs)with excellent thermal ***,the enhancement efficiency of GPCs is hindered by the agglomeration of fillers and the interfacial thermal resistance caused by the lack of continuous thermally conductive pathways between the filler and *** the interfaces between filler and matrix is of great importance in optimizing the performances of ***,we fabricated graphite nanofibers(GNF)-loaded nanocomposites using acid-functionalized GNF(AGNF)and acidtetraethylenepentamine-functionalized(TGNF)as a filler and epoxy resin as a matrix with different GNF loading contents to explore the interfacial properties of the *** optimal GNF loading for AGNF was 0.5 wt.%,while the TGNF showed 0.75 wt.%.The highest thermal conductivity(0.51 W m^(−1) K^(−1))and fracture toughness(25.8 MPa m^(1/2))values were found in the TGNF-loaded nanocomposites with a fraction of 0.75 wt.%,representing enhancements of∼145%and∼400%,respectively,compared to those of neat *** experimental data presented herein demonstrate that the interfacial properties play a significant role in enhancing the thermal and mechanical performances of the *** present approach is expected to serve as a valuable tool in the design of conductive polymeric nanocomposites for further practical applications,such as thermal interface materials and packaging of high-power electric devices.

关键词： Graphite nanofiber Nanocomposites Interfacial interaction Thermal conductivity Fracture resistance

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An efficient deep learning-assisted person re-identification solution for intelligent video surveillance in smart cities

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Frontiers of Computer Science 2023年第4期17卷 83-96页

作者： Muazzam MAQsooD Sadaf YASMIN Saira GILLANI Maryam BUKHARI Seungmin RHO sang-soo YEO Department of Computer Science COMSATS University IslamabadAttock CampusAttock 43600Pakistan Department of Computer Science Bahria UniversityLahore 54600Pakistan Department of Industrial Security Chung-Ang UniversitySeoul 06974Republic of Korea Department of Computer Engineering Mokwon UniversityDaejeon 35349Republic of Korea

Innovations on the Internet of Everything(IoE)enabled systems are driving a change in the settings where we interact in smart units,recognized globally as smart city ***,intelligent video-surveillance systems are critical to increasing the security of these smart *** precisely,in today’s world of smart video surveillance,person re-identification(Re-ID)has gained increased consideration by *** researchers have designed deep learningbased algorithms for person Re-ID because they have achieved substantial breakthroughs in computer vision *** this line of research,we designed an adaptive feature refinementbased deep learning architecture to conduct person *** the proposed architecture,the inter-channel and inter-spatial relationship of features between the images of the same individual taken from nonidentical camera viewpoints are focused on learning spatial and channel *** addition,the spatial pyramid pooling layer is inserted to extract the multiscale and fixed-dimension feature vectors irrespective of the size of the feature ***,the model’s effectiveness is validated on the CUHK01 and CUHK02 *** compared with existing approaches,the approach presented in this paper achieves encouraging Rank 1 and 5 scores of 24.6% and 54.8%,respectively.

关键词： Internet of Everything(IoE) visual surveillance systems big data security systems person re-identification(Re-ID) deep learning

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Incidence, prevalence and risk of stroke in patients with Takayasu arteritis: a nationwide population-based study in South Korea

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Stroke & Vascular Neurology 2022年第2期7卷 149-157页

作者： Sung soo Ahn Minkyung Han Yong-Beom Park Inkyung Jung sang-Won Lee Department of Internal Medicine Yongin Severance HospitalYonsei University College of MedicineYonginSouth Korea Biostatistics Collaboration Unit Department of Biomedical Systems InformaticsYonsei University College of MedicineSeoulSouth Korea Division of Rheumatology Department of Internal MedicineYonsei University College of MedicineSeoulSouth Korea Institute for Immunology and Immunological Diseases Yonsei University College of MedicineSeoulSouth Korea Division of Biostatistics Department of Biomedical Systems InformaticsYonsei University College of MedicineSeoulSouth Korea

Background Takayasu arteritis(TAK)is a disease associated with increased risk of cardiovascular *** aimed to evaluate the incidence,prevalence and risk of stroke in patients with *** Data from 1065 patients were obtained from a national database(2010–2018).The annual incidence and prevalence per 100000 persons were estimated using the registration population in the midst of every year,and the standardised incidence ratio(SIR)of stroke was compared with the general population based on the data from the 2006 national report for cardiovascular and cerebrovascular ***-adjusted incidence rate ratio(IRR)of stroke based on the time interval after diagnosis was also calculated.A time-dependent Cox regression was conducted to investigate predictive factors of *** The overall incidence rate of TAK ranged between 0.2 and 0.3/100000 person-years annually;the prevalence of TAK gradually increased,reaching 3.25/100000 person-years in ***-three(6.9%)patients experienced stroke during follow-up,and the risk of developing stroke was higher than the general population(overall SIR 7.39,95%CI 5.79 to 9.29;men:SIR 5.70,95%CI 2.84 to 10.20;women:SIR 7.06,95%CI 5.41 to 9.05).Most stroke events(90.9%)were cerebral infarction for men,whereas the proportion of cerebral infarction was lower(62.9%)in *** half of stroke events occurred within 6 months after diagnosis,and stroke was more common within 6 months of diagnosis compared with after 3 years in women(IRR 13.46,95%CI 6.86 to 26.40).In Cox regression analysis,age was the sole predictor of stroke(adjusted HR 1.02,95%CI 1.00 to 1.04,p=0.043).Conclusions The annual incidence of TAK was similar to the previous studies from Asia,and the risk of stroke increased in *** patterns of subtype and incidence of stroke were found according to sex,although age was the only predictor.

关键词： prevalence patients diagnosis

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Usefulness of transcatheter arterial embolization for eighty-three patients with secondary postpartum hemorrhage:Focusing on difference in angiographic findings

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World Journal of Clinical Cases 2023年第15期11卷 3471-3480页

作者： Bong Man Kim Gyeong Sik jeon Min jeong Choi Nam-soo Hong Department of Radiology Dankook University HospitalDankook University College of MedicineCheonan-si 31116Chungcheongnam-doSouth Korea Department of Radiology CHA Bundang Medical CenterCollege of MedicineCHA UniversitySeongnam-si 13496Gyeonggi-doSouth Korea Department of Preventive Medicine School of MedicineKyungpook National UniversityDaegu 41944South Korea

BACKGROUND Transcatheter arterial embolization(TAE)has been widely used as an effective and a safe treatment method and was often used as an alternative to the surgical management,but there are limited studies on the efficacy and the safety for patients undergoing their secondary postpartum hemorrhage(PPH).AIM To evaluate the usefulness of TAE for secondary PPH focusing on the angiographic *** We conducted a research from January 2008 to July 2022 on all 83 patients(mean:32 years,range:24-43 years)presented with secondary PPH and they were treated with TAE in two university *** medical records and angiography were retrospective reviewed in order to evaluate the patients’characteristics,delivery details,clinical status and peri-embolization management,angiography and embolization details,technical/clinical success and *** group with active bleeding sign and the group without it were also compared and *** On angiography,46(55.4%)patients showed active bleeding signs such as contrast extravasation(n=37)or pseudoaneurysm(n=8)or both(n=1),and 37(44.6%)patients showed non-active bleeding signs such as only spastic uterine artery(n=2)or hyperemia(n=35).In the active bleeding sign group there were more multiparous patients,low platelet count,prothrombin time prolongation,and high transfusion *** technical success rates were 97.8%(45/46)in active bleeding sign group and 91.9%(34/37)in non-active bleeding sign group,and the overall clinical success rates were 95.7%(44/46)and 97.3%(36/37).An uterine rupture with peritonitis and abscess formation occurred to one patient after the embolization,therefore hysterostomy and retained placenta removal were performed which was a major *** TAE is an effective and a safe treatment method for controlling secondary PPH regardless of angiographic findings.

关键词： Postpartum hemorrhage Angiography Transcatheter arterial embolization Pseudoaneurysm Nbutylcyanoacrylate

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Altered gut microbiota patterns in COVID-19:Markers for inflammation and disease severity

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World Journal of Gastroenterology 2022年第25期28卷 2802-2822页

作者： Chiranjib Chakraborty Ashish Ranjan Sharma Manojit Bhattacharya Kuldeep Dhama sang-soo Lee School of Life Science and Biotechnology Adamas UniversityKolkata 700126India Institute for Skeletal Aging&Orthopaedic Surgery Hallym UniversityChuncheon-si 24252South Korea Department of Zoology Fakir Mohan UniversityBalasore 756020India Division of Pathology Indian Council of Agricultural Research(ICAR)-Indian Veterinary Research Institute(IVRI)Bareilly 243122Uttar PradeshIndia Institute for Skeletal Aging&Orthopedic Surgery Hallym UniversityChuncheon-si 24252South Korea

The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection leads to a severe respiratory illness and alters the gut microbiota,which dynamically interacts with the human immune *** alterations include decreased levels of beneficial bacteria and augmentation of opportunistic ***,we describe critical factors affecting the microbiota in coronavirus disease 2019(COVID-19)*** include,such as gut microbiota imbalance and gastrointestinal symptoms,the pattern of altered gut microbiota composition in COVID-19 patients,and crosstalk between the microbiome and the gut-lung axis/gut-brain-lung ***,we have illustrated the hypoxia state in COVID-19 associated gut microbiota *** role of ACE2 in the digestive system,and control of its expression using the gut microbiota is discussed,highlighting the interactions between the lungs,the gut,and the brain during COVID-19 ***,we address the gut microbiota in elderly or co-morbid patients as well as gut microbiota dysbiosis of in severe *** clinical trials to understand the role of probiotics in COVID-19 patients are listed in this *** inflammation is one of the major driving forces for COVID-19 symptoms and gut microbiome disruption and is associated with disease ***,understanding the role of the gut microbiota in immune modulation during SARS-CoV-2 infection may help improve therapeutic strategies for COVID-19 treatment.

关键词： COVID-19 Inflammation Gut microbiota Therapeutic

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Curative resection of leiomyosarcoma of the descending colon with metachronous liver metastasis: A case report

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World Journal of Gastrointestinal Surgery 2023年第5期15卷 992-999页

作者： soo-Hyeon Lee sang-Ho Bae sang-Cheol Lee Tae-Sung Ahn Zisun Kim Hae-Il Jung Department of Surgery Soonchunhyang University Cheonan HospitalCheonan 31151ChungnamSouth Korea Department of Internal Medicine Soonchunhyang University Cheonan HospitalCheonan 31151ChungnamSouth Korea Department of Surgery Soonchunhyang University Bucheon HospitalBucheon 14584GyeonggiSouth Korea

BACKGROUND Leiomyosarcoma(LMS)has a poor prognosis and rarely originates from the *** resection is possible,surgery is the first treatment most commonly ***,no standard treatment exists for hepatic metastasis of LMS;although,several treatments,such as chemotherapy,radiotherapy,and surgery,have been ***,the management of liver metastases remains *** SUMMARY We present a rare case of metachronous liver metastasis in a patient with LMS originating from the descending colon.A 38-year-old man initially reported abdominal pain and diarrhea over the previous two *** revealed a 4-cm diameter mass in the descending colon,40 cm from the anal *** tomography revealed intussusception of the descending colon due to the 4-cm *** patient underwent a left *** analysis of the tumor revealed that it was positive for smooth muscle actin and desmin,and negative for cluster of differentiation 34(CD34),CD117,and discovered on gastrointestinal stromal tumor(GIST)-1,which are characteristic of gastrointestinal LMS.A single liver metastasis developed 11 mo postoperatively;the patient subsequently underwent curative resection *** patient remained disease-free after six cycles of adjuvant chemotherapy(doxorubicin and ifosfamide),and 40 and 52 mo after liver resection and primary surgery,*** cases were obtained from a search of Embase,PubMed,MEDLINE,and Google *** Early diagnosis and surgical resection may be the only potential curative options for liver metastasis of gastrointestinal LMS.

关键词： Leiomyosarcoma Colon Liver metastasis Surgical resection Treatment Case report

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Silent advanced large cell neuroendocrine carcinoma with synchronous adenocarcinoma of the colon:A case report

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World Journal of Gastrointestinal Oncology 2022年第11期14卷 2266-2272页

作者： Hyeon Seok Baek sang Wook Kim soo Teik Lee Ho Sung Park Seung Young Seo Department of Internal Medicine Research Institute of Clinical Medicine of Jeonbuk National University-Biomedical Research Institute of Jeonbuk National University HospitalJeonju-si 56445Jeollabuk-doSouth Korea Department of Pathology Jeonbuk National University Medical SchoolJeonjusi 56445Jeollabuk-doSouth Korea

BACKGROUND Large cell neuroendocrine carcinoma(LCNEC)accounts for about 0.25%of colorectal cancer ***,synchronous LCNEC and adenocarcinoma coexistence in the colon is very *** are usually aggressive and have a poor ***,colorectal LCNEC patients complain of abdominal symptoms such as pain,diarrhea or hematochezia because it is often diagnosed as an advanced disease that accompanies metastatic *** SUMMARY We describe a case of relatively asymptomatic synchronous LCNEC and colon adenocarcinoma.A 62-year-old male patient visited our hospital due to anemia detected by a local health *** did not complain of melena,hematochezia or abdominal *** examination was unremarkable and his abdomen was soft,nontender and nondistended with no palpable *** tests revealed anemia with hemoglobin 5.1 g/*** revealed an ulcerofungating lesion in the ascending colon and about a 1.5 cm-sized large sessile polyp in the sigmoid *** biopsy of the ascending colon lesion revealed the ulcerofungating mass that was LCNEC and endoscopic mucosal resection at the sigmoid colon lesion showed a large polypoid lesion that was *** liver,lung,bone and lymph nodes metastasis was found on chest/abdominal computed tomography and positron emission *** patient was diagnosed with advanced colorectal LCNEC with liver,lung,bone and lymph node metastasis(stage IV)and synchronous colonic adenocarcinoma *** this case,no specific symptom except anemia was observed despite the multiple *** patient refused systemic chemotherapy and was discharged after *** We report a case of silent LCNEC of the colon despite the advanced state and synchronous adenocarcinoma.

关键词： Large cell neuroendocrine carcinoma Colon Synchronous Adenocarcinoma Case report

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3D cell-printing of gradient multi-tissue interfaces for rotator cuff regeneration

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Bioactive Materials 2023年第1期19卷 611-625页

作者： Suhun Chae Uijung Yong Wonbin Park Yoo-mi Choi In-Ho jeon Homan Kang Jinah Jang Hak soo Choi Dong-Woo Cho Department of Mechanical Engineering Pohang University of Science and Technology77 Cheongam-roNam-guGyeongsangbuk-doPohang37673South Korea EDmicBio Inc. 111 Hoegi-roDongdaemun-guSeoul 02445South Korea Institute for Convergence Research and Education in Advanced Technology Yonsei University50 Yonsei-roSeodaemun-guSeoul03722South Korea Gordon Center for Medical Imaging Department of RadiologyMassachusetts General Hospital&Harvard Medical School14913th StreetBostonMA02114USA Department of Convergence IT Engineering Pohang University of Science and Technology77 Cheongam-roNam-guGyeongsangbuk-doPohang37673South Korea School of Interdisciplinary Bioscience and Bioengineering Pohang University of Science and Technology77 Cheongam-roNam-guGyeongsangbuk-doPohang37673South Korea Department of Orthopaedic Surgery Asan Medical CenterCollege of MedicineUniversity of Ulsan86 Asanbyeongwon-gilSongpa-guSeoul05505South Korea

Owing to the prevalence of rotator cuff(RC)injuries and suboptimal healing outcome,rapid and functional regeneration of the tendon-bone interface(TBI)after RC repair continues to be a major clinical *** the essential role of the RC in shoulder movement,the engineering of biomimetic multi-tissue constructs presents an opportunity for complex TBI reconstruction after RC ***,we propose a gradient cell-laden multi-tissue construct combined with compositional gradient TBI-specific bioinks via 3D cell-printing *** vitro studies demonstrated the capability of a gradient scaffold system in zone-specific inducibility and multi-tissue formation mimicking *** regenerative performance of the gradient scaffold on RC regeneration was determined using a rat RC repair *** particular,we adopted nondestructive,consecutive,and tissue-targeted near-infrared fluorescence imaging to visualize the direct anatomical change and the intricate RC regeneration progression in real time in ***,the 3D cell-printed implant promotes effective restoration of shoulder locomotion function and accelerates TBI healing in *** summary,this study identifies the therapeutic contribution of cell-printed constructs towards functional RC regeneration,demonstrating the translational potential of biomimetic gradient constructs for the clinical repair of multi-tissue interfaces.

关键词： 3D cell-printing Tissue-specific bioink Gradient tissue scaffolds Near-infrared fluorophores Rotator cuff repair

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Investigation of prodromal features in metabolic syndrome based on transcriptome analysis

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Genes & Diseases 2023年第3期10卷 708-711页

作者： sang-Min Park Musun Park Hyo-jeong Ban Su-Jin Baek soo Yeon Kim Siwoo Lee Hee-jeong Jin Korean Medicine(KM)Data Division Korea Institute of Oriental MedicineDaejeon 34054Republic of Korea College of Pharmacy Chungnam National UniversityDaejeon 34134Republic of Korea

Metabolic syndrome(MetS)is a complex condition that significantly increases the risk of several diseases,including heart disease and *** with MetS show both pro-inflammatory and pro-thrombotic states;however,the state... 详细信息

关键词： diseases Metabolic Patients

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