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Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

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Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim Kyeong-Hyeon Eom Dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun kim Jun-Koo Yi Jae-Jung Ha Ji-Hyun Lee Seong-Beom Lee Woo-Jae Son soo-Young Yum Won-Wu Lee Goo Jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing has been considered as powerful tool in agricultural ***,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising ***,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in *** this point of view,electroporation in embryos has been risen as an *** First,editing efficiency of our electroporation methods were tested for *** of mutation on embryo was confirmed by T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both *** These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively ***,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

Influence of overhanging tool length and vibrator material on electromechanical impedance and amplitude prediction in ultrasonic spindle vibrator

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2024年第4期25卷 292-310页

作者： Rendi KURNIAWAN Moran XU Min Ki CHOO Shuo CHEN Yein KWAK' Jielin CHEN Saood ALI Hanwei TENG Pil Wan HAN Gi soo kim Tae Jo KO Precision Machining Laboratory Department of Mechanical Engineering Yeungnam UniversityGyeongsansiGyeongbuk-doRepublic of Korea Korea Electrotechnology Research Institute(KERI) ChangwonsiGyeongsangnam-doRepublic of Korea KASWIN Co. LtdChangwon-siGyeongsangnamdoRepublic of Korea

This study presents the development of an ultrasonic transducer with a radius horn for an ultrasonic milling spindle(UMS)*** ultrasonic transducer was intended to have a working frequency of approximately 30 *** different materials were considered in the study:stainless steel(SS 316L)and titanium alloy(Ti-6Al-4V).Titanium alloy gave a higher resonance frequency(33 kHz)than stainless steel(30 kHz)under the same preload compression *** electromechanical impedance simulation was carried out to predict the impedance resonance frequency for both materials,and the effect of the overhanging toolbar was *** to the electromechanical impedance simulation,the overhanging toolbar length affected the resonance frequency,and the error was less than 3%.Harmonic analysis confirmed that the damping ratio helps determine the resonance ***,damping ratios of 0.015-0.020 and 0.005-0.020 were selected for stainless steel and titanium alloy,respectively,with an error of less than 1.5%.Experimental machining was also performed to assess the feasibility of ultrasonic-assisted milling;the result was a lesser cutting force and better surface topography of Al 6061.

关键词： Ultrasonic spindle Ultrasonic vibration assisted-milling(UVAM) 1-degree of freedom(DOF) Frequency Amplitude Milling

Exercise training mode effects on myokine expression in healthy adults:A systematic review with meta-analysis

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Journal of Sport and Health Science 2024年第6期13卷 764-779页

作者： Francesco Bettariga Dennis R.Taaffe Daniel A.Galvao Pedro Lopez Chris Bishop Anna Maria Markarian Valentina Natalucci Jin-soo kim Robert U.Newton Exercise Medicine Research Institute Edith Cowan UniversityJoondalupWA 6027Australia School of Medical and Health Sciences Edith Cowan UniversityJoondalupWA 6027Australia Pleural Medicine Unit Institute for Respiratory HealthPerthWA 6009Australia Medical School Faculty of Health&Medical SciencesUniversity of Western AustraliaPerthWA 6009Australia Grupo de Pesquisa em Exercicio para Populacoes Clinicas(GPCLIN) Universidade de Caxias do SulCaxias do SulRio Grande do Sul 95070-560Brazil London Sport Institute School of Science and TechnologyMiddlesex UniversityLondonNW44BTUK Department of Pathophysiology and Transplantation University of MilanMilan 20133Italy School of Human Movement and Nutrition Sciences University of QueenslandSt.LuciaQLD 4072Australia

Background:The benefits of exercise are well known;however,many of the underlying molecular mechanisms are not fully *** muscle secretes myokines,which mediate muscleorgan *** regulate satellite-cell proliferation and migration,inflammatory cascade,insulin secretion,angiogenesis,fatty oxidation,and cancer *** date,the effects of different exercise modes(namely,aerobic and resistance exercise)on myokine response remain to be *** is crucial considering the clinical implementation of exercise to enhance general health and wellbeing and as a medical ***:A systematic search was undertaken in PubMed,MEDLINE,CINAHL,Embase,SPORTDiscus,andWeb of Science in April *** studies examining the effects of a single bout of exercise on interleukin15(IL-15),irisin,secreted protein acidic and rich in cysteine(SPARC),oncostatinM(OSM),and decorin were included.A random-effects meta-analysis was also undertaken to quantify the magnitude of ***:Sixty-two studies were included(n=1193).Overall,exercise appeared to induce small to large increases in myokine expression,with effects observed immediately after to 60 min post-exercise,although these were mostly not statistically *** aerobic and resistance exercise resulted in changes in myokine levels,without any significant difference between training modes,and with the magnitude of change differing across *** levels returned to baseline levels within 180 min to 24 h ***,owing to potential sources of heterogeneity,most changes were not statistically significant,indicating that precise conclusions cannot be ***:Knowledge is limited but expanding with respect to the impact of overall and specific effects of exercise on myokine expression at different time points in the systemic *** research is required to investigate the effects of different exercise modes at multiple time points on myok

关键词： Myokine Resistance exercise Aerobic exercise Cytokine Systemic circulation

Nonlinear Finite Element Analysis of Ocean Cables

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海洋工程：英文版 2004年第4期18卷 537-550页

作者： Nam-Il kim sang-soo JEON Moon-Young kim Department of Civil-Environmental Engineering SungKyunKwan University Jangan-Gu Suwon Kyongki-Do 440-746 S. Korea Highway and Transportation Technology Institute Korea Highway Corporation San 28-1 Sancheok-Ri Dongtan-Myeon Hwaseong City S. Korea Department of Civil-Environmental Engineering SungKyunKwan University Jangan-Gu Suwon Kyongki-Do 440-746 S. Korea

This study has focused on developing numerical procedures for the dynamic nonlinear analysis of cable structures subjected to wave forces and ground motions in the ocean. A geometrically nonlinear finite element procedure using the isoparametric curved cable element based on the Lagrangian formulation is briefly summarized. A simple and accurate method to determine the initial equilibrium state of cable systems associated with self-weights, buoyancy and the motion of end points is presented using the load incremental method combined with penalty method. Also the Newmark method is used for dynamic nonlinear analysis of ocean cables. Numerical examples are presented to validate the present numerical method.

关键词： nonlinear cable wave finite element analysis

Huge encrusted ureteral stent forgotten for over 25 years:A case report

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World Journal of Clinical Cases 2020年第23期8卷 6043-6047页

作者： Dong soo kim sang Hyub Lee Department of Urology School of MedicineKyung Hee UniversitySeoul 02447South Korea

BACKGROUND Ureteral stent insertion is a relatively non-invasive procedure commonly used in the field of urology to resolve urinary ***,they are sometimes forgotten and the risk of complications increases with *** SUMMARY A 43-year-old woman with a history of recurrent urinary tract infections visited our hospital for evaluation of persistent left flank pain,and lower urinary tract symptoms despite anti-biotic *** had received urological surgery in her teens but did not know the details of her ***,ureter,and bladder X-ray and abdominopelvic computed tomography revealed a forgotten left ureteral stent with huge encrustation at both ends and a severely dilated left kidney with parenchymal *** order to remove the ureteral stent,a laparoscopic nephroureterectomy was *** ureteral stent was successfully removed,and she was discharged after *** patient was followed up for two years after surgery and did not show any signs of long-term *** Long indwelling stents can cause dire complications requiring radical *** registry systems,novel stent material development,and proper patient education is important for complication prevention.

关键词： Ureter Stent Calcification Endoscopy Nephroureterectomy Complications Case report

Identification of crystal symmetry from noisy diffraction patterns by a shape analysis and deep learning

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npj Computational Materials 2020年第1期6卷 35-45页

作者： Leslie Ching Ow Tiong Jeongrae kim sang soo Han Donghun kim Computational Science Research Center Korea Institute of Science and Technology(KIST)Seoul 02792Republic of Korea

The robust and automated determination of crystal symmetry is of utmost importance in material characterization and *** studies have shown that deep learning(DL)methods can effectively reveal the correlations between X-ray or electron-beam diffraction patterns and crystal *** their promise,most of these studies have been limited to identifying relatively few classes into which a target material may be *** the other hand,the DL-based identification of crystal symmetry suffers from a drastic drop in accuracy for problems involving classification into tens or hundreds of symmetry classes(e.g.,up to 230 space groups),severely limiting its practical usage.

关键词： symmetry crystal limiting

UBVVI Surface Photometry of the Spiral Galaxy NGC 300 in the Sculptor Group

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Chinese Journal of Astronomy and Astrophysics 2004年第4期4卷 299-310页

作者： sang Chul kim Hwankyung Sung Hong soo Park Eon-Chang Sung Korea Astronomy Observatory Taejon 305-348Republic of KoreaDepartment of Astronomy and Space ScienceSejong University98 Gunja-dongGwangjin-guSeoul 143-747Republic of KoreaAstronomy ProgramSEESSeoul National UniversitySeoul 151-742Republic of KoreaKorea Astronomy ObservatoryTaejon 305-348Republic of Korea

We present UBVI surface photometry over a 20.5' × 20.5' area of the late-type spiral galaxy NGC 300. We have derived isophotal maps, surface brightness profiles, ellipticity profiles, position angle profiles, and color profiles. By merging our I-band measurements with those of Boker et al. based on Hubble Space Telescope observations, we have obtained combined I-band surface brightness profiles for the region 0.02' < r < 500' and have decomposed the profiles into three components: a nucleus, a bulge, and an exponential disk.

关键词： galaxies: spiral - galaxies: photometry - galaxies: individual(NGC 300) - galaxies: nuclei

Amine functionalization on thermal and mechanical behaviors of graphite nanofibers-loaded epoxy composites

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Journal of Materials Science & Technology 2023年第20期151卷 80-88页

作者： Seong-Hwang kim sang-Jin Park Seul-Yi Lee soo-Jin Park Department of Chemistry Inha University100 InharoIncheon 22212the Republic of Korea

With the growing demand for faster and more powerful computing,effective heat dissipation is essential to ensure the longevity,reliability,and high performance of electronic *** the field of modern electronic packaging materials,there is a great need for graphitic material-loaded polymeric composites(GPCs)with excellent thermal ***,the enhancement efficiency of GPCs is hindered by the agglomeration of fillers and the interfacial thermal resistance caused by the lack of continuous thermally conductive pathways between the filler and *** the interfaces between filler and matrix is of great importance in optimizing the performances of ***,we fabricated graphite nanofibers(GNF)-loaded nanocomposites using acid-functionalized GNF(AGNF)and acidtetraethylenepentamine-functionalized(TGNF)as a filler and epoxy resin as a matrix with different GNF loading contents to explore the interfacial properties of the *** optimal GNF loading for AGNF was 0.5 wt.%,while the TGNF showed 0.75 wt.%.The highest thermal conductivity(0.51 W m^(−1) K^(−1))and fracture toughness(25.8 MPa m^(1/2))values were found in the TGNF-loaded nanocomposites with a fraction of 0.75 wt.%,representing enhancements of∼145%and∼400%,respectively,compared to those of neat *** experimental data presented herein demonstrate that the interfacial properties play a significant role in enhancing the thermal and mechanical performances of the *** present approach is expected to serve as a valuable tool in the design of conductive polymeric nanocomposites for further practical applications,such as thermal interface materials and packaging of high-power electric devices.

关键词： Graphite nanofiber Nanocomposites Interfacial interaction Thermal conductivity Fracture resistance

Asymmetric optical camouflage:tuneable reflective colour accompanied by the optical Janus effect

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Light(Science & Applications) 2020年第1期9卷 325-334页

作者： Taehyun kim Eui-sang Yu Young-Gyu Bae Jongsu Lee In soo kim Seok Chung Seung-Yeol Lee Yong-sang Ryu Sensor System Research Center Korea Institute of Science and TechnologySeoul 02792Republic of Korea Department of Micro/Nano Systems Korea UniversitySeoul 02841Republic of Korea School of Electronic and Electrical Engineering Kyungpook National UniversityDaegu 41566Republic of Korea Nanophotonics Research Center Korea Institute of Science and TechnologySeoul 02792Republic of Korea School of Mechanical Engineering Korea UniversitySeoul 02841Republic of Korea

Going beyond an improved colour gamut,an asymmetric colour contrast,which depends on the viewing direction,and its ability to readily deliver information could create opportunities for a wide range of applications,such as nextgeneration optical switches,colour displays,and security features in anti-counterfeiting ***,we propose a simple Fabry–Perot etalon architecture capable of generating viewing-direction-sensitive colour contrasts and encrypting pre-inscribed information upon immersion in particular solvents(optical camouflage).Based on the experimental verification of the theoretical modelling,we have discovered a completely new and exotic optical phenomenon involving a tuneable colour switch for viewing-direction-dependent information delivery,which we define as asymmetric optical camouflage.

关键词： asymmetric generating readily