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Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast

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Synthetic and Systems Biotechnology 2020年第1期5卷 11-18页

作者： Tadas Jakočiūnas Andreas K.Klitgaard Eftychia Eva Kontou Julie Bang Nielsen Emil Thomsen David Romero-Suarez Kai Blin Christopher J.Petzold Jennifer W.Gin Yaojun Tong Charlotte Held Gotfredsen Pep Charusanti Rasmus J.N.Frandsen Tilmann Weber sang yup lee Michael K.Jensen Jay D.Keasling The Novo Nordisk Foundation Center for Biosustainability Technical University of DenmarkKgs.LyngbyDenmark Joint BioEnergy Institute EmeryvilleCAUSA Biological Systems&Engineering Division Lawrence Berkeley National LaboratoryBerkeleyCAUSA Department of Chemical and Biomolecular Engineering&Department of Bioengineering University of California BerkeleyCAUSA Center for Synthetic Biochemistry Institute for Synthetic BiologyShenzhen Institutes for Advanced TechnologiesShenzhenChina Department of Chemistry Technical University of DenmarkKgs.LyngbyDenmark Department of Biotechnology and Biomedicine Technical University of DenmarkKgs.LyngbyDenmark Department of Chemical and Biomolecular Engineering(BK21 Plus Program) Korea Advanced Institute of Science and Technology(KAIST)291 Daehak-roYuseong-guDaejeon34141Republic of Korea

To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms,development of new,versatile,bio-based production platforms is required.Here we present a novel yeast-based platform for biosynthesis of bacterial aromatic polyketides.The platform is based on a synthetic polyketide synthase system enabling a first demonstration of bacterial aromatic polyketide biosynthesis in a eukaryotic host.

关键词： Natural products Synthetic biology Aromatic polyketides CRISPR/Cas9 Polyketide Production platform in eukaryotes Metabolic engineering Biotechnology

Crystallization Proess for Highly Purified Succinic Acid from Fermentation Broth

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Crystallization Proess for Highly Purified Succinic Acid fro...

Delayed-onset endophthalmitis associated with Achromobacter species developed in acute form several months after cataract surgery:Three case reports

The 12th Asian Pacific Confederation of Chemical Engineering Congress

作者： Yeon Ki Hong Eun Zoo lee Yun Suk Huh Young-Si Jun Won Hi Hong sang yup lee Department of Chemical and Biological Engineering Chungju National University 72 Daehak-roChungjuChungbuk 380-702Korea Department of Chemical and Biomolecular Engineering KAIST 373-1 Guseong-dongYuseong-guDaejeon 305-701Korea

Succinic acid is a four-carbon dicarboxylic acid produced as one of the fermentation products of anaerobic metabolism.Based on the complete genome sequence of a capnophific succinic acid- producing

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World Journal of Clinical Cases 2022年第19期10卷 6496-6500页

作者： Tae-Hoon Kim sang-Joon lee Ki-yup Nam Department of Ophthalmology Kosin University HospitalBusan 49267South Korea Department of Ophthalmology Kosin UniversityCollege of MedicineBusan 49267South Korea Department of Ophthalmology Chungnam National UniveristyCollege of MedicineDaejeon 35015South Korea Department of Ophthalmology Chungnam National University Sejong HospitalSejong 30099South Korea

BACKGROUND Achromobacter species-associated endophthalmitis is rare and may present as either acute or chronic postoperative endophthalmitis.Delayed-onset Achromobacter species endophthalmitis appearing in acute presentation that develops more than several months after cataract surgery is very rare.Intraocular lens(IOL)removal is commonly recommended to treat Achromobacter species endophthalmitis,which is based on previous studies.Here,we report the results of surgery without IOL removal when treating patients with delayed-onset postoperative Achromobacter species endophthalmitis that developed in an acute form.CASE SUMMARY Three patients visited our ophthalmology clinic due to visual impairment that began 2-3 d earlier.They had undergone cataract surgery 5-18 mo prior.Bestcorrected visual acuity of the diseased eye was between counting fingers at 30 cm to non-light perception.They showed conjunctival injection,inflammation in the anterior chamber(cell reaction 4+)and hypopyon formation.The patients were diagnosed with infectious endophthalmitis and immediately underwent pars plana vitrectomy,anterior chamber irrigation and intravitreal injection of ceftazidime and vancomycin.Before fluid infusion,a vitreous specimen was obtained. In all cases, the IOLs were not removed. Achromobacter species was detected on vitreousspecimen culture. After surgery, the vitreous opacity decreased gradually and there was littleretinal damage. At 1 mo after treatment, the best-corrected visual acuity had improved to 20/50and 20/40.CONCLUSIONDelayed onset postoperative endophthalmitis caused by Achromobacter species can appear in anacute form. All patients responded well to early vitrectomy and administration of empiricalantibiotics including ceftazidime. There was no need for IOL removal during surgery.

关键词： Achromobacter species Chronic endophthalmitis Postoperative endophthalmitis Case report

Water hammer in the pump-rising pipeline system with an air chamber

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Journal of Hydrodynamics 2014年第6期26卷 960-964页

作者： KIM sang-Gyun lee Kye-Bock KIM Kyung-yup Korea Water Resources Corporation School of Mechanical Engineering Chungbuk National University School of Mechanical Engineering Korea Polytechnic University

Water hammer following the tripping of pumps can lead to overpressure and negative pressure. Reduction in overpressure and negative pressure may be necessary to avoid failure, to improve the efficiency of operation and to avoid fatigue of system components. The field tests on the water hammer have been conducted on the pump rising pipeline system with an air chamber. The hydraulic transient was simulated using the method of characteristics. Minimizing the least squares problem representing the difference between the measured and predicted transient response in the system performs the calibration of the simulation program. Among the input variables used in the water hammer analysis, the polytropic exponent, the discharge coefficient and the wave speed were calibrated. The computer program developed in this study will be useful in designing the optimum parameters of an air chamber for the real pump pipeline system. The correct selection of air chamber size and the effect of the inner diameter of the orifice to minimize water hammer have been investigated by both field measurements and numerical modeling.

关键词： air chamber,design parameter,field test,pump pipeline system,water hammer

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全基因组合成技术

环球科学 2020年第23期 33-33页

作者：安德鲁·黑塞尔李相烊龚聪(翻译) 不详

在新冠疫情全球大流行的初期,中国科学家就迅速完成了新冠病毒的测序工作,并将基因序列上传到了公开的遗传物质数据库。紧接着,瑞士的一个科研小组迅速合成了整个基因组并依此制造出了病毒。这就像是直接把病毒样本发送给瑞士的实验室... 详细信息

在新冠疫情全球大流行的初期,中国科学家就迅速完成了新冠病毒的测序工作,并将基因序列上传到了公开的遗传物质数据库。紧接着,瑞士的一个科研小组迅速合成了整个基因组并依此制造出了病毒。这就像是直接把病毒样本发送给瑞士的实验室一样。因此,欧洲的科学家根本不需要经过漫长而严苛的实体病毒寄送过程就能开展研究。这样的速度就是全基因组合成技术推动医学和其他领域发展的一个例证。

关键词：数据库科研小组病毒样本实验室遗传物质基因序列科学家全球大流行

Development of the Separate Air-Supply Type of Pressurization Smoke Control System for the Stairwells of High-Rise Buildings in Korea

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Open Journal of Fluid Dynamics 2014年第3期4卷 251-262页

作者： Jung-yup Kim Hyun-Joon Shin Chan-Sol Ahn Ji-Seok Kim sang-Hyun Joo Fire Research Center Korea Institute of Civil Engineering and Building Technology Goyang Korea

The pressurization smoke control system has been commonly used as a smoke control system at the emergency stairs of high-rise buildings. However, a higher possibility of overpressure between the lobby and the accommodation or pressure drop in the lobby could lead to failure in achievement of the purpose of pressurization system, particularly when supplying the leakage and supplementary air flow through one air-supply path at a time. To improve this particular issue, the devise configurations, as well as the different ways to supply the leakage and supplementary air flow through the different flow passages have been proposed. The performance of the trial product was evaluated on the test bed, ultimately providing a safe evacuation environment if high-rise buildings fired.

关键词： Pressurization System Smoke Control High-Rise Building Evacuation Stairwell Field Experiment

抗生素FR-008基因簇上游区域的DNA序列分析

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农业生物技术学报 2005年第3期13卷 404-405页

作者：陈实,周秀芬,JEONG Hae-Young,CHOI Kang-Ryul,lee sang-yup,邓子新韩国大田305-701 韩国大田305-701 上海200030 上海交通大学生命科学技术学院Bio-X生命科学中心上海交通大学生命科学技术学院Bio-X生命科学中心韩国Genotech公司韩国Genotech公司韩国高等科技学院化学及生物分子工程系上海交通大学生命科学技术学院Bio-X生命科学中心上海200030 韩国高等科技学院化学及生物分子工程系韩国大田305-701 上海200030 韩国大田305-701

链霉菌FR-008产生一种七烯大环内酯类抗生素F R-008,对真菌具有很高的抗菌活性,并对蚊子幼虫有高毒性(袁德军和周启,1990)。负责合成FR-008的基因簇被定位(H uetal.,1994),有21个基因(共138kb,G enB ankaccessionnum ber A Y310323)被... 详细信息

链霉菌FR-008产生一种七烯大环内酯类抗生素F R-008,对真菌具有很高的抗菌活性,并对蚊子幼虫有高毒性(袁德军和周启,1990)。负责合成FR-008的基因簇被定位(H uetal.,1994),有21个基因(共138kb,G enB ankaccessionnum ber A Y310323)被确认参与了抗生素FR-008的生物合成以及调节

关键词：链霉菌 FR-008基因簇基因分析

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2019年全球十大新兴技术

环球科学 2019年第23期 22-33页

作者：玛丽特·迪克里斯蒂娜伯纳德·S·迈耶森哈维尔·加西亚·马丁内斯施怿(翻译) 科琳娜·E·莱森杰弗里·凌阿尔贝托·莫斯卡泰利戴晨(翻译) 伊丽莎白·奥戴马一瑷(翻译) 杰夫·卡贝克安徳鲁·梅纳德罗娜·钱徳拉瓦提马克·菲谢蒂李相烨安徳烈亚·汤普森不详

《科学美国人》联合世界经济论坛,评选出了2019年全球十大新兴技术。这些新兴技术正蓄势待发,它们有望改变现状,在生物技术、环境能源、工程机械等领域引发变革。在新兴技术不断涌现的今天,有哪些新生力量将在未来几年脱颖而出,革新我... 详细信息

《科学美国人》联合世界经济论坛,评选出了2019年全球十大新兴技术。这些新兴技术正蓄势待发,它们有望改变现状,在生物技术、环境能源、工程机械等领域引发变革。在新兴技术不断涌现的今天,有哪些新生力量将在未来几年脱颖而出,革新我们的生活方式?

关键词：世界经济论坛工程机械新兴技术环境能源改变现状新生力量全球

尼可地尔在经皮冠状动脉介入治疗不稳定心绞痛时对心肌的保护作用

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首都食品与医药 2017年第22期24卷 160-160页

作者： Ju Han Kim Myung Ho Jeong Kyung Ho Yun Kye Hun Kim Dong Koo Kang Seo Na Hong sang yup Lim sang Hyun lee Yun sang lee Young Joon Hong Hyung Wook Park Weon Kim Young Keun Ahn Jeong Gwang Cho Jong Chun Park Jung Chaee Kang 田玉敬北京四环科宝制药有限公司 100070

心肌损伤通常在经皮冠状动脉介入治疗(PCI)过程中出现,并且损伤可以通过对心肌酶的测试使之容易被监控。众所周知,尼可地尔是三磷酸腺苷ATP-敏感的K+通道的开启器,通过减小作用电位从而具有抗心律失常效应和保护心脏作用。比如,通过松... 详细信息

心肌损伤通常在经皮冠状动脉介入治疗(PCI)过程中出现,并且损伤可以通过对心肌酶的测试使之容易被监控。众所周知,尼可地尔是三磷酸腺苷ATP-敏感的K+通道的开启器,通过减小作用电位从而具有抗心律失常效应和保护心脏作用。比如,通过松弛血管平滑肌以减少微冠状动脉血流阻力。研究尼可地尔的心肌保护作用,IONA研究(尼可地尔对心绞痛的作用)即大规模对稳定型心绞痛进行的研究表明,尼可地尔口服给药能够减少冠心病致死率和非致命型心肌梗塞或胸痛意外住院治疗。目前已经对数个不稳定心绞痛(UAP)或急性心肌梗塞病人进行了尼可地尔心脏保护效应的研究评估,但还没有进行大规模研究。此研究的目的在于通过对经尼可地尔注射并PCI治疗12~48h后的不稳定心绞痛患者的心肌酶进行评估来预期考察尼可地尔的心脏保护作用。

关键词：尼可地尔介入治疗不稳定心绞痛心肌保护