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格陵兰NEEM冰芯1711~1969年火山事件重建

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第四纪研究 2021年第3期41卷 726-738页

作者：杜志恒 Soon Do Hur 效存德杨佼林佳梅 Yeongcheol Han 张通李传金任贾文王士猛 Seong Joon Jun sang bum hong Khanghyun Lee 中国科学院西北生态环境资源研究院冰冻圈科学国家重点实验室甘肃兰州730000 Korea Polar Research Institute Incheon 21990Korea 北京师范大学地表过程与资源生态国家重点实验室北京100875 Physics of Ice Climate and EarthNiels Bohr InstituteUniverisity of CopenhagenCopenhagen N 2200Denmark

冰芯作为良好的地质载体,为第四纪气候和环境研究提供了详实的信息。本研究利用连续流动装置对格陵兰冰盖西北部获取的一支90 m浅冰芯(命名为NEEM2009S1:77.45°N,51.06°W;海拔高度2450 m)融化处理,对氧和氢同位素比率(δ^(18)O和δD,2... 详细信息

冰芯作为良好的地质载体,为第四纪气候和环境研究提供了详实的信息。本研究利用连续流动装置对格陵兰冰盖西北部获取的一支90 m浅冰芯(命名为NEEM2009S1:77.45°N,51.06°W;海拔高度2450 m)融化处理,对氧和氢同位素比率(δ^(18)O和δD,2 cm分辨率)和硫酸根离子浓度(SO_(4)^(2-),10~12 cm分辨率)进行了测试。基于δ^(18)O和关键的强火山事件对该冰芯进行了定年,结果为1711~1969年(不确定性为±1年);通过非海盐硫酸根离子浓度(nss SO_(4)^(2-))重建了该冰芯过去1711~1969年期间火山事件的历史。SO_(4)^(2-)浓度和通量很好地揭示了不同强度大小的火山事件。评估了强火山事件(VEI≥5)其产生的气候制冷效应;同时,由于该冰芯距离冰岛较近,本研究重点讨论了该冰芯对冰岛火山事件的记录历史,数据结果对了解冰岛历史时期火山信息具有重要的意义;此外,低纬度东亚与阿拉斯加地区的火山也容易传输至格陵兰冰盖。重建结果对于更好的理解火山事件对区域、全球环境与气候的影响具有重要的参考价值。

关键词：格陵兰冰盖 NEEM冰芯火山事件冰岛气候效应

Sensitivity to electricity consumption in urban business and commercial area buildings according to climatic change

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Journal of Central South University 2012年第3期19卷 770-776页

作者： LEE Kang-guk KIM Sung-bum hong Won-hwa Research Center for Urban Affair Kyungil University School of Architecture Kyungpook National University

Recently, urban high temperature phenomenon has become a problem which results from human activities, the increase in energy consumption, and land-cover change in urban areas. As extremely hot weather caused by urban high temperature continues, demand for power is increased and results in the degradation of electricity reserves. The current trend in climate change, regardless of the summer and winter power demand, is likely to have much effect on the power demand. Thus, sensitivity to electricity consumption in urban areas due to climate change was researched. The results show that, 1） the basic unit of the sensitivity to electricity consumption in the target areas is 1.25-1.58W/（m2.℃）; 2） The maximum sensitivity is recorded at around 8：00 pm in the area crowded with commercial and business area. And in the business area, electricity consumption load is even from 9：00 am to 6：00 pm.

关键词： electricity consumption electricity load electricity reserves energy

X region mutations of hepatitis B virus related to clinical severity

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World Journal of Gastroenterology 2016年第24期22卷 5467-5478页

作者： hong Kim Seoung-Ae Lee bum-Joon Kim Department of Biomedical Sciences Microbiology and Immunology Liver Research Institute and Cancer Research Institute College of Medicine Seoul National University

Chronic hepatitis B virus (HBV) infection remains a major health problem, with more than 240 million people chronically infected worldwide and potentially 650000 deaths per year due to advanced liver diseases including liver cirrhosis and hepatocellular carcinoma (HCC). HBV-X protein (HBx) contributes to the biology and pathogenesis of HBV via stimulating virus replication or altering host gene expression related to HCC. The HBV X region contains only 465 bp encoding the 16.5 kDa HBx protein, which also contains several critical cis-elements such as enhancer II, the core promoter and the microRNA-binding region. Thus, mutations in this region may affect not only the HBx open reading frame but also the overlapped cis-elements. Recently, several types of HBx mutations significantly associated with clinical severity have been described, although the functional mechanism in most of these cases remains unsolved. This review article will mainly focus on the HBx mutations proven to be significantly related to clinical severity via epidemiological studies.

关键词： Hepatitis B virus infection Hepatitis B virus-X protein mutation Hepatocellular carcinoma Clinical severity

Development of Fok-I based nested polymerase chain reaction-restriction fragment length polymorphism analysis for detection of hepatitis B virus X region V5M mutation

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World Journal of Gastroenterology 2015年第47期21卷 13360-13367页

作者： hong Kim Seok-Hyun hong Seoung-Ae Lee Jeong-Ryeol Gong bum-Joon Kim Department of Biomedical Sciences Microbiology and Immunology and Liver Research Institute Seoul National University College of Medicine

AIM: To develop a Fok-I nested polymerase chain reaction(PCR)-restriction fragment length polymorphism analysis(PRA) method for the detection of hepatitis B virus X region(HBx) V5 M ***: Nested PCR was applied into DNAs from 198 chronic patients at 2 different stages [121 patients with hepatocellular carcinoma(HCC) and 77 carrier patients]. To identify V5 M mutants, digestion of nested PCR amplicons by the restriction enzyme Fok-I(GGA TGN9↓) was done. For size comparison, the enzymetreated products were analyzed by electrophoresis on 2.5% agarose gels, stained with ethidium bromide, and visualized on a UV ***: The assay enabled the identification of 69 patients(sensitivity of 34.8%; 46 HCC patients and 23 carrier patients). Our data also showed that V5 M prevalence in HCC patients was significantly higher than in carrier patients(47.8%, 22/46 patients vs 0%, 0/23 patients, P < 0.001), suggesting that HBx Ag V5 M mutation may play a pivotal role in HCC generation in chronic patients with genotype C ***: The Fok-I nested PRA developed in this study is a reliable and cost-effective method to detect HBx Ag V5 M mutation in chronic patients with genotype C2 infection.

关键词： Hepatitis B virus X antigen Polymerasechain reaction-restriction fragment length polymorphismanalysis V5M mutation Hepatocellur carcinoma

AME证据系列001--转化医学协会:脓毒症诊断和早期识别的临床实践指南

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临床与病理杂志 2016年第10期36卷 1467-1476页

作者： Zhongheng Zhang Nathan J.Smischney Haibo Zhang Sven Van Poucke Panagiotis Tsirigotis Jordi Rello Patrick M.Honore Win Sen Kuan Juliet June Ray Jiancang Zhou You Shang Yuetian Yu Christian Jung Chiara Robba Fabio Silvio Taccone Pietro Caironi David Grimaldi Stefan Hofer George Dimopoulos Marc Leone sang-bum hong Mabrouk Bahloul Laurent Argaud Won Young Kim Herbert D.Spapen Jose Rodolfo Rocco 张建成(译) 尚游(译) 钟鸣(校) Department of Emergency Medicine Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016 Department of Critical Care Medicine Jinhua Hospital of Zhejiang UniversityJinhua Zhejiang 321000 Department of Anesthesiology Mayo ClinicRochesterMNUSA Keenan Research Center for Biomedical Science of St.Michael's Hospital Departments of Anesthesia and PhysiologyInterdepartmental Division of Critical Care MedicineUniversity of TorontoTorontoONCanada Departments of Anesthesiology Critical CareEmergency Medicine and Pain TherapyZiekenhuis Oost-Limburgy GenkBelgium 2nd Department of Internal Medicine Attikcon University HospitalMedical SchoolNational and Kapodistrian University of AthensAthensGreece CIBERES Vall d'Hebron Institut of ResearchUniversitat Autonoma de BarcelonaSpain Universitair Ziekenhuis Brussely Vrije Universiteit Brussel Brussels BrusselsBelgium Emergency Medicine Department National University HospitalNational University Health SystemSingapore and the Department of SurgeryYong Loo Lin School of MedicineNational University of SingaporeSingapore DeWitt Daughtry Family Department of Surgery Jackson Memorial HospitalUniversity of Miami Leonard M.Miller School of MedicineMiamiFLUSA Department of Critical Care Medicine Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016 Department of Critical Care Medicine Union HospitalTongji Medical CollegueHuazhong University of Science and TechnologyWuhan 430022 Department of Critical Care Medicine Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200001 University Hospital Dusseldorf Heinrich-Heine-University DusseldorfMedical FacultyDivision of CardiologyPulmonology and Vascular MedicineDusseldorfGermany Neurosciences Critical Care Unit Addenbrooke's HospitalCambridgeUK Department of Intensive Care Hopital ErasmeUniversite Libre de Bruxelles(ULB)BrusselsBelgium Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti Fondazione IRCCS Ca'Granda-Ospedale

脓毒症是一种由感染引起的异质性疾病,感染触发了一系列复杂的局部或者全身的免疫炎症反应,引起多器官功能衰竭,发病率和病死率显著升高。由于至今仍然没有诊断脓毒症的金标准,所以脓毒症的临床诊断仍是一个难题。因此,脓毒症的临床诊... 详细信息

脓毒症是一种由感染引起的异质性疾病,感染触发了一系列复杂的局部或者全身的免疫炎症反应,引起多器官功能衰竭,发病率和病死率显著升高。由于至今仍然没有诊断脓毒症的金标准,所以脓毒症的临床诊断仍是一个难题。因此,脓毒症的临床诊断需要不断改变来满足临床和研究的要求。然而,尽管有许多新型的生物标记和筛选工具去预测脓毒症发生的风险,但是这些措施的诊断价值和有效性不足以让人满意,并且没有充分的证据去建议临床使用这些新技术。因此,脓毒症的临床诊断标准需要定期更新去适应不断产生的新证据。这篇综述旨在呈现当前脓毒症的诊断和早期识别方面的最新研究证据。临床运用不同的诊断方法的推荐意见依赖于推荐、评价、发展和评估分级体系(Grades of Recommendation Assessment,Development and Evaluation,GRADE),因为大部分的研究是观察性研究,并没有对这些方法进行可靠评估,采用的是两步推理方法。未来需要更多研究来确认或者反驳某一特殊的指标检测,同时应该直接采用相关病人的结果数据。

关键词：脓毒症早期识别诊断

Precore/core region mutations of hepatitis B virus related to clinical severity

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World Journal of Gastroenterology 2016年第17期22卷 4287-4296页

作者： hong Kim Seoung-Ae Lee Seung Yeon Do bum-Joon Kim Department of Biomedical Sciences Microbiology and Immunology Liver Research Institute and Cancer Research Institute College of Medicine Seoul National University

Despite the availability of an effective vaccine, hepatitis B virus (HBV) infection remains a major health problem, with more than 350 million chronically infected people worldwide and over 1 million annual deaths due to cirrhosis and liver cancer. HBV mutations are primarily generated due both to a lack of proofreading capacity by HBV polymerase and to host immune pressure, which is a very important factor for predicting disease progression and therapeutic outcomes. Several types of HBV precore/core (preC/C) mutations have been described to date. The host immune response against T cells drives mutation in the preC/C region. Specifically, preC/C mutations in the MHC class II restricted region are more common than in other regions and are significantly related to hepatocellular carcinoma. Certain mutations, including preC G1896A, are also significantly related to HBeAg-negative chronic infection. This review article mainly focuses on the HBV preC/C mutations that are related to disease severity and on the HBeAg serostatus of chronically infected patients.

关键词： Hepatitis B virus infection Precore/core mutations Hepatocellular carcinoma HBeAg serostatus Disease severity

Colonic J-pouch anal anastomosis after ultralow anterior resection with upper sphincter excision for low-lying rectal cancer

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World Journal of Gastroenterology 2005年第17期11卷 2570-2573页

作者： Jae-Gahb Park Min Ro Lee Seok-Byung Lim Chang Won hong sang Nam Yoon Sung-bum Kang Seung Chul Heo Seung-Yong Jeong Kyu Joo Park Department of Surgery Seoul National University College of Medicine 28 Yongon-dongChongno-guSeoul 110-744Korea Cancer Research Institute and Cancer Research CenterSeoul National University28 Yongon-dongChongno-guSeoul 110-744Korea Research Institute and HospitalNational Cancer Center809 Madu-dongIlsan-guGoyangGyeonggi 411-764Korea Department of Surgery Seoul National University College of Medicine 28 Yongon-dongChongno-guSeoul 110-744Korea Research Institute and Hospital National Cancer Center 809 Madu-dongIlsan-guGoyangGyeonggi 411-764Korea

AIM:There is some evidence of functional superiority of colonic J-pouch over straight coloanal anastomosis (CM) in ultralow anterior resection (ULAR) or intersphincteric resection. On the assumption that colonic J-pouch anal anastomosis is superior to straight CM in ULAR with upper sphincter excision (USE: excision of the upper part of the internal sphincter) for low-lying rectal cancer, we compare functional outcome of colonic J-pouch vsthe straight CM. METHODS: Fifty patients of one hundred and thirty-three rectal cancer patients in whom lower margin of the tumors were located between 3 and 5 cm from the anal verge received ULAR including USE from September 1998 to January 2002. Patients were randomized for reconstruction using either a straight (n = 26) or a colonic J-pouch anastomosis (n = 24) with a temporary diverting-loop ileostomy. All patients were followed-up prospectively by a standardized questionnaire [Fecal Inco-ntinence Severity Index (FISI) scores and Fecal Incontinence Quality of Life (FIQL) scales]. RESULTS: We found that, compared to straight anastomosis patients, the frequency of defecation was significantly lower in J-pouch anastomosis patients for 10 mo after ileostomy takedown. The FISI scores and FIQL scales were significantly better in J-pouch patients than in straight patients at both 3 and 12 mo after ileostomy takedown. Furthermore, we found that FISI scores highly correlated with FIQL scales. CONCLUSION: This study indicates that colonic J-pouch anal anastomosis decreases the severity of fecal incontinence and improves the quality of life for 10 mo after ileostomy takedown in patients undergoing ULAR with USE for low-lying rectal cancer.

关键词： Upper sphincter excision Ultralow anterior resection Coloanal anastomosis Rectal cancer

Induction of endoplasmic reticulum-derived oxidative stress by an occult infection related S surface antigen variant

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World Journal of Gastroenterology 2015年第22期21卷 6872-6883页

作者： In-Kyung Lee Seoung-Ae Lee hong Kim You-Sub Won bum-Joon Kim Department of Microbiology and Immunology Liver Research Institute Cancer Research Institute and SNUMRCCollege of Medicine Seoul National University

AIM: To investigate the mechanism of endoplasmic reticulum(ER) stress induction by an occult infection related hepatitis B virus S surface antigen(HBsAg)***: We used an HBsAg variant with lower secretion capacity, which was a KD variant from a Korean subject who was occultly infected with the genotype C. We compared the expression profiles of ER stress-related proteins between HuH-7 cells transfected with HBsAg plasmids of a wild-type and a KD variant using Western ***: Confocal microscopy indicated that the KD variant had higher levels of co-localization with ER than the wild-type HBsAg. The KD variant upregulated ER stress-related proteins and induced reactive oxygen species(ROS) compared to the wildtype via an increase in calcium. The KD variant also down-regulated anti-oxidant proteins(HO-1, catalase and SOD) compared to the wild-type, which indicates positive amplification loops of the ER-ROS axis. The KD variant also induced apoptotic cell death via the upregulation of caspase proteins(caspase 6, 9 and 12).Furthermore, the KD variant induced a higher level of nitric oxide than wild-type HBsAg via the up-regulation of the iNOS ***: Our data indicate that occult infection related HBsAg variants can lead to ER-derived oxidative stress and liver cell death in HuH-7 cells.

关键词： Endoplasmic reticulum oxidative stress Hepatitis B virus KD variant Colocalization Reactive oxidative species Apoptotic cell death

Occult infection related hepatitis B surface antigen variants showing lowered secretion capacity

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World Journal of Gastroenterology 2015年第6期21卷 1794-1803页

作者： hong Kim Seoung-Ae Lee You-Sub Won Hyun Joo Lee bum-Joon Kim Department of Biomedical Sciences Microbiology and Immunology and Liver Research Institute Seoul National University College of Medicine

AIM:To elucidate the molecular mechanisms underlying hepatitis B virus(HBV)occult infection of genotype ***:A total of 10 types of hepatitis B surface antigen(HBs Ag)variants from a Korean occult cohort were *** a complete HBV genome plasmid mutated such that it does not express HBs Ag and plasmid encoding,each HBs Ag variant was transiently co-transfected into Hu H-7 *** secretion capacity and intracellular expression of the HBV virions and HBs Ags in their respective variants were analyzed using real-time quantitative polymerase chain reaction assays and commercial HBs Ag enzyme-linked immunosorbent assays,***:All variants exhibited lower levels of HBs Ag secretion into the medium compared with the wild *** particular,in eight of the ten variants,very low levels of HBs Ag secretion that were similar to the negative control were *** contrast,most variants(9/10)exhibited normal virion secretion capacities comparable with,or even higher than,the wild *** provided new insight into the intrinsic nature of occult HBV infection,which leads to HBs Ag sero-negativeness but has horizontal ***,most variants generated higher reactive oxidative species production than the wild *** finding provides potential links between occult HBV infection and liver disease ***:The presently obtained data indicate that deficiency in the secretion capacity of HBs Ag variants may have a pivotal function in the occult infections of HBV genotype C.

关键词： Occult infection Hepatitis B virus Hepatitis B sur