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HISP-1 Inhibits HSv-1 Infection in Cultured vero Cells

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Advances in Bioscience and Biotechnology 2024年第4期15卷 269-288页

作者： sandra D. Adams Kevin T. Bilyk Modukuri v. Ramani Gottumukkala v. Subbaraju Department of Biology Montclair State University Montclair USA Department of Chemistry Andhara University Visakhapatnam India

Herpes simplex virus-1 (HSv-1) remains a leading cause of viral disease worldwide and is spread by direct contact with infected lesions. There is no vaccine against HSv-1 infections and there remains a need to identify therapeutics that could reduce the spread. In this study various hispolon compounds were analyzed to determine their antiviral potential against HSv-1 infections in cultured vero cells. To determine the effects on infectivity and possible mechanisms of inhibition, the following assays were conducted. In vitro cytotoxicity assays were conducted to determine the effect of the compounds on cell viability and the maximum non-cytotoxic concentrations. Antiviral assays measured cell viability, percent inhibition of infection following treatment with the compounds, and the effect on the viral infection cycle. These effects were visualized using inverted light and fluorescent microscopy. Of the 24 hispolons tested, only hispolon pyrazole-1 (HISP-1) demonstrated antiviral effects. HISP-1 was demonstrated to effect early stages in HSv-1 infection in cultured vero cells (attachment, penetration, and post-penetration). In silico modeling analyses were conducted to analyze the interactions between HISP-1 and viral glycoprotein D (gD). HISP-1 is safe at concentrations tested and is effective in inhibiting infection of HSv-1 in cultured cells. HISP-1 has potential for therapeutic use as an antiviral against HSv-1 infection, could work in synergy with other antivirals that work be a different modality, and could be developed as a component of a topical agent to reduce the spread of HSv-1 infections.

关键词： Hispolon Herpes Simplex virus-1 Antiviral Curcumin

Exploring the effects of dietary inulin in rainbow trout fed a high‑starch,100%plant‑based diet

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Journal of Animal Science and Biotechnology 2024年第2期15卷 713-732页

作者： Raphaël Defaix Jep Lokesh Laura Frohn Mickael Le Bechec Thierry Pigot vincent veron Anne Surget sandra Biasutti Frederic Terrier Sandrine Skiba-Cassy Jerome Roy Stephane Panserat Karine Ricaud Universite de Pau Et Des Pays de L’Adour E2S UPPAINRAENUMEASaint‑Pee‑Sur‑NivelleFrance Universite de Pau Et Des Pays de L’Adour E2S UPPACNRSIMT Mines AlesIPREMPauFrance Universite de Pau Et Des Pays de L’Adour E2S UPPAIUT des Pays de l’AdourDepartement Genie BiologiqueMont de MarsanFrance.

Background High dietary carbohydrates can spare protein in rainbow trout(Oncorhynchus mykiss)but may affect growth and ***,a prebiotic,could have nutritional and metabolic effects,along with anti-inflammatory properties in teleosts,improving growth and *** tested this hypothesis in rainbow trout by feeding them a 100%plant-based diet,which is a viable alternative to fishmeal and fish oil in aquaculture *** a two-factor design,we examined the impact of inulin(2%)as well as the variation in the carbohydrates(CHO)/plant protein ratio on rainbow *** assessed the influence of these factors on zootechnical parameters,plasma metabolites,gut microbiota,production of short-chain fatty acids and lactic acid,as well as the expression of free-fatty acid receptor genes in the mid-intestine,intermediary liver metabolism,and immune markers in a 12-week feeding *** The use of 2%inulin did not significantly change the fish intestinal microbiota,but interestingly,the high CHO/protein ratio group showed a change in intestinal microbiota and in particular the beta diversity,with 21 bacterial genera affected,including Ralstonia,Bacillus,and 11 lactic-acid producing *** were higher levels of butyric,and valeric acid in groups fed with high CHO/protein diet but not with *** high CHO/protein group showed a decrease in the expression of pro-inflammatory cytokines(il1b,il8,and tnfa)in liver and a lower expression of the genes coding for tight-junction proteins in mid-intestine(tjp1a and tjp3).However,the 2%inulin did not modify the expression of plasma immune ***,inulin induced a negative effect on rainbow trout growth performance irrespective of the dietary *** With a 100%plant-based diet,inclusion of high levels of carbohydrates could be a promising way for fish nutrition in aquaculture through a protein sparing effect whereas the supplementation of 2%inulin does not appear to improve the use of CHO wh

关键词： Aquaculture Fish nutrition Gut microbiota Immune markers Intermediary metabolism Inulin Prebiotic Rainbow trout Short-chain fatty acids

Supramolecular Polymerization of Hydrogen-Bonded Trimers in Bulk and Aqueous Medium

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CCS Chemistry 2024年第6期6卷 1458-1465页

作者： Freek v.de Graaf Stef A.H.Jansen sandra M.C.Schoenmakers Bas van Genabeek Marle E.J.vleugels Bart W.L.van den Bersselaar E.W.Meijer Ghislaine vantomme Institute for Complex Molecular Systems Laboratory of Macromolecular and Organic ChemistryEindhoven University of Technology5600 MBEindhoven School of Chemistry and RNA Institute University of New South WalesSydneyNew South Wales 2052

Synthetic molecules that form functional noncovalent assemblies in water have received much attention for their potential applications as *** this work,we introduce a novel water-compatible monomer based on the phthalhydrazide moiety that assembles into hydrogen-bonded trimeric *** bulk,the material exhibits a cylindrical nanostructure in the liquid-crystalline phase at elevated temperatures,morphologically distinct from the crystalline lamellar *** water,these molecules effectively form cylinders in a one-dimensional fashion,yielding fibrous *** formation of these supramolecular polymers follows a cooperative mechanism,as evidenced by denaturation *** trimerized pattern represents a new category of aqueous su pramolecular polymers with future prospects for functional complex molecular systems.

关键词： noncovalent chemistry supramolecular polymers hydrogen bonding cooperativity nanostructure

Antimicrobial properties of sea anemone Anthopleura nigrescens from Pacific coast of Costa Rica

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Asian Pacific Journal of Tropical Biomedicine 2016年第5期6卷 418-421页

作者： Henry Borbn sandra v.ldes Javier Alvarado-Mesn Roy Soto Ilena vega Laboratory of Research and Development in Chemical Technology Department of ChemistryNational University Laboratory of Biochemistry and Biotechnology of Proteins Department of Biological ScienceNational University Laboratory of Natural Products and Biological Essays Department of ChemistryNational University

Objective: To evaluate the antimicrobial and antifungal activities of the aqueous and partitioned extract of sea anemone Anthopleura nigrescens(A. nigrescens).Methods: The sea anemone A. nigrescens was collected, minced, homogenized,lyophilized and then further partitioned with diethyl ether, acetone, ethanol and *** fractions were evaluated for antimicrobial activity against bacterial and fungal ***: Acetone extract was found to produce a pronounced inhibition of 7.0 mm against Proteus vulgaris and diethyl ether extract inhibited Pseudomonas aeruginosa with an inhibition zone of 6.5 mm. In antifungal activity, ethanol extract showed good activity against Botrytis cinerea, Trichoderma harzianum and Rhizopus oryzae compared with other strains. Acetone and ethanol extract of A. nigrescens showed activity against all of pathogens tested. Slight activity was observed in the water extract with inhibition zone of 1.5 ***: The present study revealed that sea anemone A. nigrescens may also contain some biologically active agents which have potential activity against pathogenic microorganisms.

关键词： Biological activity Antibacterial Antifungal Bioactive Sea anemone

Scientific guidelines for preclinical research on potentised preparations manufactured according to current pharmacopoeias-the PrePoP guidelines

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Journal of Integrative Medicine 2024年第5期22卷 533-544页

作者： Alexander L.Tournier Leoni v.Bonamin Susann Buchheim-Schmidt Steven Cartwright Christoph Dombrowsky Paul Doesburg Carla Holandino Maria Olga Kokornaczyk Everine Bvan de Kraats Jesús Antonio López-Carvallo Papiya Nandy JoséManuel Mazón-Suástegui Fateme Mirzajani Bernard Poitevin Claudia Scherr Karin Thieves sandra Würtenberger Stephan Baumgartner Institute of Complementary and Integrative Medicine University of Bern3010 BernBernSwitzerland Institute of Integrative Medicine Witten/Herdecke University58455 WittenNorth Rhine–WestphaliaGermany Homeopathy Research Institute London SW74EFUnited Kingdom Graduate Program on Environmental and Experimental Pathology Paulista UniversitySão Paulo 01504-000São PauloBrazil Department of Medical Science Deutsche Homöopathie-Union DHU-Arzneimittel GmbH&Co.KG76227 KarlsruheBaden-WürttembergGermany Cherwell Laboratory for Fundamental Research in Homeopathy Oxford OX28NUOxforeshireUnited Kingdom Society for Cancer Research Hiscia Research Institute4144 ArlesheimBaselSwitzerland Laboratório Multidisciplinar de Ciências Farmacêuticas Universidade Federal do Rio de JaneiroRio de Janeiro 21941-902Rio de JaneiroBrazil World Water Community Rotterdam 3029AKthe Netherlands Departamento de Acuicultura Facultad de Ciencias del MarUniversidad Católica del Norte1790437 CoquimboElquiChile Centre for Interdisciplinary Research and Education KolkataWest Bengal 700068West BengalIndia Laboratorio de Homeopatía Acuícola y Semillas Marinas Centro de Investigaciones Biológicas del NoroesteLa Paz 23096Baja California SurMexico Protein Research Center Shahid Beheshti UniversityTehran ***Iran Department of Medical Laboratory Science College of ScienceKnowledge University44001 ErbilErbilIraq SociétéSavante d’Homéopathie 75009 ParisFrance Sola Salus Institute for Homeopathic Research52511 GeilenkirchenNorth Rhine–WestphaliaGermany Scientific Society for Homeopathy 06366 KöthenSaxony-AnhaltGermany

Objective Pharmacopoeias regulate the manufacture of potentised pharmaceutical preparations used in different branches of complementary and integrative *** physicochemical properties and biological activity of these preparations are often investigated in preclinical research,yet no guidelines for experimental research currently exist in this *** present PrePoP guidelines aim to provide recommendations to promote high-quality,statistically sound,and reproducible preclinical research on potentised *** Input was gathered from researchers nominated by the relevant scientific societies using a simplified Delphi consensus approach covering the most relevant aspects of basic research methodology in the field including appropriate controls,sample preparation and handling,and *** three rounds of feedback,a consensus was finally reached on the most important aspects and considerations for conducting high-quality research on potentised *** We present a series of recommendations on a range of topics including experimental controls,system stability,blinding and randomisation,environmental influences,and procedures for the preparation of potentised samples and controls,and we address some specific challenges of this research *** This expert consensus process resulted in a robust set of methodological guidelines for research on potentised preparations and provides a valuable framework that will inform and improve the quality of subsequent research in this emerging field.

关键词： Research guidelines Fundamental research Basic research Complementary and integrative medicine Potentisation Potentised preparations

The acceleration of a high-charge electron bunch to 10 Gev in a 10-cm nanoparticle-assisted wakefield accelerator

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Matter and Radiation at Extremes 2024年第1期9卷 15-24页

作者： Constantin Aniculaesei Thanh Ha Samuel Yoffe Lance Labun Stephen Milton Edward McCary Michael M.Spinks Hernan J.Quevedo Ou Z.Labun Ritwik Sain Andrea Hannasch Rafal Zgadzaj Isabella Pagano Jose A.Franco-Altamirano Martin L.Ringuette Erhart Gaul Scott v.Luedtke Ganesh Tiwari Bernhard Ersfeld Enrico Brunetti Hartmut Ruhl Todd Ditmire sandra Bruce Michael E.Donovan Michael C.Downer Dino A.Jaroszynski Bjorn Manuel Hegelich University of Texas at Austin AustinTexas 78712USA SUPA Department of Physics University of StrathclydeGlasgowScotland G4 ONGUnited Kingdom Tau Systems Inc.AustinTexas 78701USA Lawrence Livermore National Laboratory LivermoreCalifornia 94550USA Los Alamos National Laboratory Los AlamosNew Mexico 87545USA Brookhaven National Laboratory UptonNew York 11973USA Ludwig-Maximilians-Universität MunichGermany

An intense laser pulse focused onto a plasma can excite nonlinear plasma *** appropriate conditions,electrons from the background plasma are trapped in the plasma wave and accelerated to ultra-relativistic *** scheme is called a laser wakefield *** this work,we present results from a laser wakefield acceleration experiment using a petawatt-class laser to excite the wakefields as well as nanoparticles to assist the injection of electrons into the accelerating phase of the *** find that a 10-cm-long,nanoparticle-assisted laser wakefield accelerator can generate 340 pC,10±1.86 Gev electron bunches with a 3.4 Gev rms convolved energy spread and a 0.9 mrad rms *** can also produce bunches with lower energies in the 4–6 Gev range.

关键词： acceleration nanoparticle charge

Colorectal cancer vaccines: Tumor-associated antigens vs neoantigens

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World Journal of Gastroenterology 2018年第48期24卷 5418-5432页

作者： sandra Wagner Christina S Mullins Michael Linnebacher Section of Molecular Oncology and Immunotherapy General SurgeryUniversity Medical Center

Therapeutic options for the treatment of colorectal cancer(CRC) are diverse but still not always satisfying. Recent success of immune checkpoint inhibition treatment for the subgroup of CRC patients suffering from hypermutated tumors suggests a permanent role of immune therapy in the clinical management of CRC. Substantial improvement in treatment outcome could be achieved by development of efficient patient-individual CRC vaccination strategies. This mini-review summarizes the current knowledge on the two general classes of targets: tumor-associated antigens(TAAs) and tumorspecific antigens. TAAs like carcinoembryonic antigen and melanoma associated antigen are present in and shared by a subgroup of patients and a variety of clinical studies examined the efficacy of different TAA-derived peptide vaccines. Combinations of several TAAs as the next step and the development of personalized TAA-based peptide vaccines are discussed. Improvements of peptidebased vaccines achievable by adjuvants and immunestimulatory chemotherapeutics are highlighted. Finally, we sum up clinical studies using tumor-specific antigens-in CRC almost exclusively neoantigens-which revealed promising results; particularly no severe adverse events were reported so far. Critical progress for clinical outcomes can be expected by individualizing neoantigen-based peptide vaccines and combining them with immunestimulatory chemotherapeutics and immune checkpoint inhibitors. In light of these data and latest developments, truly personalized neoantigen-based peptide vaccines can be expected to fulfill modern precision medicine's requirements and will manifest as treatment pillar for routine clinical management of CRC.

关键词： Cancer vaccines Colorectal neoplasm Immunotherapy Neoplasm antigen tumor-associated antigens tumor-specific antigens Neoantigen(s)

Enhancing endogenous stem cells in the newborn via delayed umbilical cord clamping

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Neural Regeneration Research 2015年第9期10卷 1359-1362页

作者： Christopher Lawton sandra Acosta Nate Watson Chiara Gonzales-Portillo Theo Diamandis Naoki Tajiri Yuji Kaneko Paul R.Sanberg Cesar v.Borlongan Center of Excellence for Aging and Brain Repair Department of Neurosurgery and Brain Repair University of South Florida College of Medicine

There is currently no consensus among clinicians and scientists over the appropriate or optimal timing for umbilical cord clamping. However, many clinical studies have suggested that delayed cord clamping is associated with various neonatal benefits including increased blood volume, reduced need for blood transfusion, increased cerebral oxygenation in pre-term infants, and decreased frequency of iron deficiency anemia in term infants. Human umbilical cord blood con- tains significant amounts of stem and progenitor cells and is currently used in the treatment of several life-threatening diseases. We propose that delayed cord clamping be encouraged as it en- hances blood flow from the placenta to the neonate, which is accompanied by an increase supply of valuable stem and progenitor cells, as well as may improve blood oxygenation and increase blood volume, altogether reducing the infant＇s susceptibility to both neonatal and age-related diseases.

关键词： stem cells umbilical cord blood neonates regenerative medicine

Marginal zone B cells are naturally reactive to collagen type II and are involved in the initiation of the immune response in collagen-induced arthritis

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Cellular & Molecular Immunology 2011年第4期8卷 296-304页

作者： Cecilia Carnrot Kajsa E Prokopec Kristina Rasbo Mikael CI Karlsson sandra Kleinau Department of Cell and Molecular Biology Uppsala UniversityUppsalaSweden Department of Medicine Unit of Clinical Allergy ResearchThe Karolinska InstituteSolnaSweden

Antibodies against type II collagen(CII)are essential for development of collagen-induced arthritis(CIA),but how and where the B-cell response to CII is initiated is not fully *** show here that naive DBA/1 mice display naturally reactive IgM and IgG anti-CII producing B cells prior to *** CII-reactive B cells were observed in the spleen and recognized as marginal zone(MZ)B *** CII immunization,CII-specific B cells expanded rapidly in the spleen,in contrast to the lymph nodes,with the initial response derived from MZ B cells and later by follicular(FO)B *** was evident despite that the MZ B cells were subject to stringent tolerance mechanisms by having a greater Fc gamma receptor IIb expression than the FO B ***,the MZ B cells migrated to the FO areas upon immunization,possibly providing antigen and activating FO T cells and subsequently FO B ***,around CIA onset increased numbers of IgG anti-CII producing FO B cells was seen in the spleen,which was dominated by IgG2a-and IgG2b-positive *** data demonstrate that CII-reactive MZ B cells are present before and expand after CII immunization,suggesting an initiating role of MZ B cells in the development of CIA.

关键词： arthritis B cells collagen type II ELISpot marginal zone mice