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A comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method

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Asian Journal of Andrology 2016年第6期18卷 840-843页

作者： Jesus L Yaniz Sara Capistros sandra vicente-fiel Carlos O Hidalgo Pilar Santolaria TECNOGAM research group Environmental Sciences Institute (IUCA) Department of Animal Production and Food Sciences University of Zaragoza Huesca Spain Animal Genetics and Reproduction Area Regional Agrifood Research and Development Service (SERIDA) 33394 Deva Gijan Spain

The aim of this study was to compare the sperm nuclear and acrosomal morphometry of three species of domestic artiodactyls; cattle （Bos taurus）, sheep （Ovis aries）, and pigs （Sus scrofa）. Semen smears of twenty ejaculates from each species were fixed and labeled with a propidium iodide-Pisum sativum agglutinin （PI/PSA） combination. Digital images of the sperm nucleus, acrosome, and whole sperm head were captured and analyzed. The use of the PI/PSA combination and CASA-Morph fluorescence-based method allowed the capture, morphometric analysis, and differentiation of most sperm nuclei, acrosomes and whole heads, and the assessment of acrosomal integrity with a high precision in the three species studied. For the size of the head and nuclear area, the relationship between the three species may be summarized as bull 〉 ram 〉 boar. However, for the other morphometric parameters （length, width, and perimeter）, there were differences in the relationships between species for sperm nuclei and whole sperm heads. Bull sperm acrosomes were clearly smaller than those in the other species studied and covered a smaller proportion of the sperm head. The acrosomal morphology, small in the bull, large and broad in the sheep, and large, long, and with a pronounced equatorial segment curve in the boar, was species-characteristic. It was concluded that there are clear variations in the size and shape of the sperm head components between the three species studied, the acrosome being the structure showing the most variability, allowing a clear distinction of the spermatozoa of each species.

关键词： artiodactyls computer-assisted sperm morphometry analysis fluorescence microscopy image analysis sperm morphometry

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Comparison of different statistical approaches to evaluate morphometric sperm subpopulations in men

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Asian Journal of Andrology 2016年第6期18卷 819-823页

作者： Jesus L Yaniz sandra vicente-fiel Caries Soler Pilar Recreo Teresa Carretero Araceli Bono Jose M Berne Pilar Santolaria TECNOGAM research group Environmental Sciences Institute (IUCA) Department of Animal Production and Food Sciences Higher Polytechnic School University of Zaragoza Ctra. Cuarte S/N 22071 Huesca Spain Department of Functional Biology and Physical Anthropology University of Valencia 46100 Burjassot Valencia Spain R+D Department Proiser R+D Scientific Park University of Valencia 46980 Paterna Valencia Spain TECNOGAM research group Environmental Sciences Institute (IUCA) Departament of Human Anatomy and Histology Faculty of Health and Sport Sciences of Huesca Plaza Universidad 3 22002 Huesca Spain TECNOGAM research group Environmental Sciences Institute (IUCA) Hospital General San Jorge de Huesca Area de Urologla Av. Martfnez de Velasco 36 22004 Huesca Spain

This study was designed to characterize morphometric sperm subpopulations in normozoospermic men by using different statistical methods and examining their suitability to classify correctly different sperm nuclear morphologies present in human ejaculates. Ejaculates from 21 normozoospermic men were collected for the study. After semen collection and analysis, samples were prepared for morphometric determination. At least 200 spermatozoa per sample were assessed for sperm morphometry by computer-assisted sperm morphometry analysis （CASA-Morph） using fluorescence. Clustering and discriminant procedures were performed to identify sperm subpopulations from the morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three morphometric subpopulations （large-round 30.4%, small-round 46.6%, and large-elongated 22.9%）. In the second analysis, using discriminant methods, the classification was made independently of size and shape. Three morphological categories according to nuclear size （small 〈10.90 μm^2, intermediate 10.91-13.07 μm^2, and large 〉13.07 μm^2） and four categories were defined on 400 canonical cells （100 × 4） from 10 men according to sperm nuclear shape （oval, pyriform, round, and elongated）. Thereafter, the resulting classification functions were used to categorize 4200 spermatozoa from 21 men. Differences in the class distribution were observed among men from both clustering and discriminant procedures. It was concluded that the combination of CASA-Morph fluorescence-based technology with multivariate cluster or discriminant analyses provides new information on the description of different morphometric sperm subpopulations in normal individuals, and that important variations in the distribution of morphometric sperm subpopulations may exist between men, with possible functional implications.

关键词： computer-assisted sperm morphometry analysis man sperm subpopulations

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Computer-assisted sperm morphometry fluorescence-based analysis has potential to determine progeny sex

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Asian Journal of Andrology 2016年第6期18卷 858-862页

作者： Pilar Santolaria Alfredo Pauciullo Miguel A Silvestre sandra vicente-fiel Leyre Villanova Alain Pinton Juan Viruel Ester Sales Jesus L Yaniz TECNOGAM research group Environmental Sciences Institute (IUCA) Department of Animal Production and Food Sciences Higher Polytechnic School University of Zaragoza Ctra. Cuarte SIN 22071 Huesca Spain Department of Agriculture Forest and Food Sciences University of Torino Largo Paolo Braccini 2 10095 Grugliasco TO Italy Department of Functional Biology and Physical Anthropology University of Valencia 469100 Burjassot Valencia Spain UMR 1388 INRA-ENVT INPT Animal Cytogenetis GenPhySE National veterinary school of Toulouse 23 Chemin des Capelles 31076 Toulouse Cedex 3 France Mediterranean Institute of marine and terrestrial Biodiversity and Ecology (IMBE) University of Aix Marseille Chemin de la Batterie des Lions 13007 Marseille France TECNOGAM research group Environmental Sciences Institute (IUCA) Department of Agricultural and Environmental Sciences Higher Polytechnic School University of Zaragoza Ctra. Cuarte S/N 22071 Huesca Spain

This study was designed to determine the ability of computer-assisted sperm morphometry analysis （CASA-Morph） with fluorescence to discriminate between spermatozoa carrying different sex chromosomes from the nuclear morphometrics generated and different statistical procedures in the bovine species. The study was divided into two experiments. The first was to study the morphometric differences between X- and Y-chromosome-bearing spermatozoa （SX and SY, respectively）. Spermatozoa from eight bulls were processed to assess simultaneously the sex chromosome by FISH and sperm morphometry by fluorescence-based CASA-Morph. SX cells were larger than SY cells on average （P 〈 0.001） although with important differences between bulls. A simultaneous evaluation of all the measured features by discriminant analysis revealed that nuclear area and average fluorescence intensity were the variables selected by stepwise discriminant function analysis as the best discriminators between SX and SY. In the second experiment, the sperm nuclear morphometric results from CASA-Morph in nonsexed （mixed SX and SY） and sexed （SX） semen samples from four bulls were compared. FISH allowed a successful classification of spermatozoa according to their sex chromosome content. X-sexed spermatozoa displayed a larger size and fluorescence intensity than nonsexed spermatozoa （P 〈 0.05）. We conclude that the CASA-Morph fluorescence-based method has the potential to find differences between X- and Y-chromosome-bearing spermatozoa in bovine species although more studies are needed to increase the precision of sex determination by this technique.

关键词： bull computer-assisted sperm morphometry analysis sexing sperm morphometry

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科研评价改革与科研人员“计量智慧”的异质性（英文）

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Journal of Data and Information Science 2025年第1期 47-73+232-233页

作者： sandra Rousseau Cinzia Daraio CEDON KU Leuven DIAG Sapienza University of Rome

目的：本研究旨在衡量科研人员对文献计量指标的认知和态度差异，重点是探索各学科内部和学科之间在“计量智慧”上的异质性。设计/方法：本研究通过对欧洲最古老、最大的综合性大学之一——罗马大学的科研人员进行探索性调查，评估其... 详细信息

目的：本研究旨在衡量科研人员对文献计量指标的认知和态度差异，重点是探索各学科内部和学科之间在“计量智慧”上的异质性。设计/方法：本研究通过对欧洲最古老、最大的综合性大学之一——罗马大学的科研人员进行探索性调查，评估其“计量智慧”。我们使用李克特5点量表评估态度，并分析了近期科研评价改革相关的文件，探讨这些改革如何考虑科研人员对于文献计量指标的异质性态度和认知。发现：研究发现，科研人员在“计量智慧”方面存在显著的学科间异质性。此外，在每个学科内部，也同时存在对文献计量指标的支持者和批评者。在文献调研中，我们没有发现关于明确提及科研人员在“计量智慧”方面个体差异的研究。研究局限性：我们使用意大利一所大学的自选科研人员样本作为探索性案例，未来的研究需要验证这些发现的普遍适用性。实践意义：为了获得对科研评价实践的充分支持，考虑科研人员对计量指标的多样化态度是关键。原创性/价值：本研究通过实证测量，为当前科研评价改革的讨论提供了新的视角，探讨了科研人员对文献计量指标的认知和态度，并讨论了这些结果对改进当前科研评价系统的重要性。

关键词：科研评估科研改革计量智慧研究人员异质性文献计量指标研究人员的态度

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Mating Disruption of Striped Rice Stem Borer: Importance of Early Deployment of Dispensers and Impact on Airborne Pheromone Concentration

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Rice science 2021年第6期28卷 525-528,I0027-I0029页

作者： Aitor GAVARA sandra VACAS Jaime PRIMO vicente NAVARRO-LLOPIS Centre for Agricultural Chemical Ecology Mediterranean Agroforestry InstitutePolytechnic University of ValenciaBuilding 6CCamino de Vera s/n46022 ValenciaSpain

Mating disruption(MD)is an effective environmentally-friendly control method against the striped rice stem borer(RSB),Chilo suppressalis(Lepidoptera:Crambidae).In this study,the current MD dispensers release the pheromone exponentially,with higher initial release rates that decrease throughout the *** adjust the timing of dispensers'deployment and evaluate the importance of controlling the early first or the second male flight,field trials were carried out to test the efficacy of two strategies:the early dispenser deployment prior to the first male flight(May)and the late deployment prior to the second flight(June).The early dispenser deployment was more effective in reducing plant damage inflicted by RSB when assessed at ***,neither of these strategies was able to inhibit the male catches of the third flight due to the dispensers5 *** to the airborne pheromone concentrations obtained,around 1.60 ng/m3 were capable to inhib社*** male flight effectively.

关键词： release Impact adjust

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DET1-mediated COP1 regulation avoids HY5 activity over second-site gene targets to tune plant photomorphogenesis

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Molecular Plant 2021年第6期14卷 963-982页

作者： Esther Canibano Clara Bourbousse Marta Garcia-Leon Borja Garnelo Gomez Lea Wolff Camila Garcia-Baudino Rosa Lozano-Duran Fredy Barneche vicente Rubio sandra Fonseca Centro Nacional de Biotecnología CNB-CSICMadrid 28049Spain Institut de biologie de l’École normale supérieure(IBENS) École normale supérieureCNRSINSERMUniversitéPSLParis 75005France Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China Department of Plant Biochemistry Centre for Plant Molecular Biology(ZMBP)Eberhard Karls University72076 TübingenGermany

DE-ETIOLATED 1(DET1)and CONSTITUTIVE PHOTOMORPHOGENESIS 1(COP1)are two essential repressors of Arabidopsis *** proteins can associate with CULLIN4 to form independent CRL4-based E3 ubiquitin ligases that mediate the degradation of several photomorphogenic transcription factors,including ELONGATED HYPOCOTYL 5(HY5),thereby controlling multiple gene-regulatory *** extensive biochemical and genetic analyses of their multi-subunit complexes,the functional links between DET1 and COP1 have long remained ***,we report that DET1 associates with COP1 in vivo,enhances COP1-HY5 interaction,and promotes COP1 destabilization in a process that dampens HY5 protein *** regulating its accumulation,DET1 avoids HY5 association with hundreds of second-site genomic loci,which are also frequently targeted by the skotomorphogenic transcription factor PHYTOCHROME-INTERACTING FACTOR ***,ectopic HY5 chromatin enrichment favors local gene repression and can trigger fusca-like *** study therefore shows that DET1-mediated regulation of COP1 stability tunes down the HY5 cistrome,avoiding hyper-photomorphogenic responses that might compromise plant viability.

关键词： HY5 DET1 COP1 photomorphogenesis light signaling fusca

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Assessment of food supplementation and surveillance as techniques to reduce damage caused by black capuchin monkeys Sapajus nigritus to forest plantations

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Current Zoology 2014年第5期60卷 581-590页

作者： sandra Bos MIKICH Laboratory of Ecology (Laborat6rio de Ecologia) Embrapa Florestas Caixa Postal 319 CEP 83411-000 Colombo PR Brazil Black Capuchin Monkey Program (Programa Macaco-prego) National Fund for the Control of Forest Pests (Fundo Nacional de Controle de Pragas Florestais) Dieter Liebsch Environmental Consulting (Dieter Liebsch Consultoria Ambiental) Curitiba PR Brazil dieterliebsch@yahoo.com.br

Black capuchin monkeys Sapajus nigritus are among several mammalian species that damage forest plantations, pri- marily plantations of pine trees （Pinus spp.） in South Brazil. Management practices to reduce these damages include supplemen- tary food offerings and plantation surveillance. To assess the efficiency of these techniques, four treatments were evaluated： feeding （F, with supplementary bananas and maize）, surveillance （S, in situ, wherein men patrolled the plantations, deterring the monkeys）, feeding ＋ surveillance （FS） and control （no procedures）（C）. The efficiency assessment of each treatment involved the number of recorded primate sightings in the treatments with the presence of guards （S and FS） and a damage inventory of the outskirts of the experimental points immediately before and after the experiment. These data were complemented by a simulta- neous study of the availability of fruit, the primary item in the diet of this primate species, in the remnant native forest in the study area. Most sightings of S. nigritus occurred early in the morning, and in general, sightings were inversely proportional to the availability of native fruits. This trend was most evident in treatment FS, most likely due to the presence of food. Treatments F and FS exhibited higher percentages of damage, indicating that these are inefficient management techniques. Treatment S exhi- bited the lowest damage rate, but at a high cost, making this technique difficult to apply over large expanses. The development of other management techniques, such as environmental enrichment and the use of deterrents, is recommended to minimize the damage caused by capuchins in forest plantations [Current Zoology 60 （5）： 581-590, 2014].

关键词： Cebus Damage control Wildlife management Pinus Food provisioning

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Pork Meat Quality of Preto Alentejano and Commercial Largewhite Landrace Cross

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Journal of Integrative Agriculture 2013年第11期12卷 1961-1971页

作者： Alfredo Teixeira sandra Rodrigues Veterinary and Animal Reserch Centre (CECAV) Agrarian School of Polytechnic Institute of BraganaCampus Sta Apolónia Apt.1172Bragan a 5301-855Portugal Mountain Research Centre(CIMO) Agrarian School of Polytechnic Institute of BraganaCampus Sta Apolónia Apt.1172Bragan a 5301-855Portugal

This work aimed to evaluate the physical-chemical characteristics of pork from a local breed （Portuguese black pork called Preto Alentejano） and a commercial breed. Sixteen animals were used, 4 females and 4 males from each breed. Animals had 80-100 kg of live weight. The longissimus muscle between the 5th thoracic vertebra and the 10th lumbar vertebra was used in the analysis. Samples were analysed for protein, fat, pigments, ashes, dry mater, water-holding capacity, and texture. Results of fat and pigments contents indicate signiifcant （P〈0.05 and P〈0.01） differences for all treatments. For protein, ashes, dry mater, water-holding capacity and texture, no signiifcant differences were found for sex. In the analysis of fatty acids composition, ten were detected, being the main ones C16：0, C18：0, C16：1, C18：1, C18：2. There was a predominance of monounsaturated fatty acids （MUFA）, followed by saturated （SFA） and polyunsaturated （PUFA）. Differences were signiifcant for sex （P〈0.01） and breed （P〈0.01）. Preto Alentejano breed and females presented the higher percentages of SFA （P〈0.01） and MUFA （P〈0.001） fatty acids. Differences between breeds might be due to breed production system and feed differences.

关键词： breed sex pork meat quality

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Hyper-chaos encryption using convolutional masking and model free unmasking

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Chinese Physics B 2014年第5期23卷 161-166页

作者：齐国元 sandra Bazebo Matondo Department of Electrical Engineerin Tshwane University of Technology Pretoria

In this paper, during the masking process the encrypted message is convolved and embedded into a Qi hyper-chaotic system characterizing a high disorder degree. The masking scheme was tested using both Qi hyper-chaos and Lorenz chaos and indicated that Qi hyper-chaos based masking can resist attacks of the filtering and power spectrum analysis, while the Lorenz based scheme fails for high amplitude data. To unmask the message at the receiving end, two methods are proposed. In the first method, a model-free synchronizer, i.e. a multivariable higher-order differential feedback controller between the transmitter and receiver is employed to de-convolve the message embedded in the receiving signal. In the second method, no synchronization is required since the message is de-convolved using the information of the estimated derivative.

关键词： Qi hyper-chaos masking high-order differentiator chaos-based encryption

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Compaction analysis and optimisation of convex-faced pharmaceutical tablets using numerical techniques

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Particuology 2019年第6期17卷 10-21页

作者： Ahmad Baroutaji sandra Lenihan Keith Bryan School of Engineering Faculty of Science and EngineeringUniversity of WolverhamptonTelfordUK School of Mechanical Electrical and Process EngineeringCork Institute of TechnologyCorkIreland

Capping failure,edge chipping,and non-uniform mechanical properties of convex-faced pharmaceutical tablets are common problems in the pharma *** this paper,the finite element method(FEM)and design of experiment techniques are used to determine the optimal shape of convex-faced(CF)pharmaceutical tablet which has more uniform mechanical properties and less capping and chipping *** effects of different geometrical parameters and friction on the compaction responses of convex-faced pharmaceutical tablets were first identified and *** FEM model of the tabletting process was generated using the implicit code ABAQUS and validated against experimental *** surface methodology was used to establish the relationship between the design variables,represented by the geometrical parameters and friction coefficient,and compaction responses of interest including residual die pressure,relative density variation within a tablet,and relative shear stress at the edge of a tablet.A statistical-based optimisation approach was then used to optimise the shape of CF *** obtained results demonstrated how the geometrical parameters and friction coefficient of CF pharmaceutical tablets strongly affect their compaction behaviour and quality.

关键词： Capping failure Edge chipping Tabletting FEM Optimisation Residual radial pressure

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