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Method for hull-less barley transformation and manipulation of grain mixed-linkage beta-glucan

Journal of Integrative Plant Biology 2018年第5期60卷 382-396页

作者： Wai Li Lim Helen M. Collins Rohan R. Singh Natalie A. J. kibble Kuok Yap Jillian Taylor Geoffrey B. Fincher Rachel A. Burton ARC Centre of Excellence in Plant Cell Walls School of Agriculture Food and Wine University of Adelaide Waite Campus Glen Osmond SouthAustralia 5064 Australia Institute for Molecular Bioscience The University of Queensland，4072，Australia

Hull-less barley is increasingly offering scope for breeding grains with improved characteristics for human nutrition; however, recalcitrance of hull-less cultivars to transformation has limited the use of these varieties. To overcome this limitation, we sought to develop an effective transformation system for hull-less barley using the cultivar Torrens. Torrens yielded a transformation efficiency of 1.8%, using a modified Agrobacterium transformation method. This method was used to over-express genes encoding synthases for the important dietary fiber component, 0,3;1,4）-β-glucan （mixed-linkage glucan）, primarily present in starchy endosperm cell walls. Over-expression of the HvCslF6 gene, driven by an endosperm-specific promoter, produced lines where mixed-linkage glucan content increased on average by 45%, peaking at 70% in some lines, with smaller increases in transgenic HvCsIH1 grain. Transgenic HvCslF6 lines displayed alterations where grain had a darker color, were more easily crushed than wild type and were smaller. This was associated with an enlarged cavity in the central endosperm and changes in cell morphology, including aleurone and sub-aleurone cells. This work provides proof-of-concept evidence that mixed-linkage glucan content in hull-less barley grain can be increased by over-expression of the HvCslF6 gene, but also indicates that hull-less cultivars may be more sensitive to attempts to modify cell wall composition.

关键词： Tobias I. Baskin University of Massachusetts USA

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Enduring alterations in hippocampal astrocytesynaptic proximity following adolescent alcohol exposure: reversal by gabapentin

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Neural Regeneration Research 2020年第8期15卷 1496-1501页

作者： Kati L.Healey sand.a kibble Sierra Hodges Kathryn J.Reissner Anze Testen Tiffany A.Wills Shawn K.Acheson Benjamin M.Siemsen John A.McFaddin Michael D.Scofield H.Scott Swartzwelder Department of Psychiatry and Behavioral Sciences Duke University Medical CenterDurhamNCUSA Department of Psychology and Neuroscience University of North CarolinaChapel HillNCUSA Department of Cell Biology and Anatomy LSU School of MedicineNew OrleansLAUSA Department of Anesthesia and Perioperative Medicine Medical University of South CarolinaCharlestonSCUSA

Adolescent alcohol abuse is a substantive public health problem that has been the subject of intensive study in recent *** reports of a wide range of effects of adolescent intermittent ethanol(AIE)exposure on brain and behavior,little is known about the mechanisms that may underlie those effects,and even less about treatments that might reverse *** studies from our laboratory have indicated that AIE produced enduring changes in astrocyte function and synaptic activity in the hippocampal formation,suggesting the possibility of an alteration in astrocyte-neuronal connectivity and *** utilized astrocyte-specific,membrane restricted viral labeling paired with immunohistochemistry to perform confocal single cell astrocyte imaging,three-dimensional reconstruction,and quantification of astrocyte morphology in hippocampal area CA1 from adult rats after ***,we assessed the colocalization of astrocyte plasma membrane labeling with immunoreactivity for AMPA-(α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)glutamate receptor 1,an AMPA receptor subunit and established neuronal marker of excitatory synapses,as a metric of astrocyte-synapse *** significantly reduced the colocalization of the astrocyte plasma membrane with synaptic marker puncta in *** is striking in that it suggests not only an alteration of the physical association of astrocytes with synapses by AIE,but one that lasts into adulthood-well after the termination of alcohol *** even more notable,the AIE-induced reduction of astrocyte-synapse interaction was reversed by sub-chronic treatment with the clinically used agent,gabapentin(Neurontin),in *** suggests that a medication in common clinical use may have the potential to reverse some of the enduring effects of adolescent alcohol exposure on brain *** animal experiments conducted were approved by the Duke University Institutional Animal Care and Use Committee(Protocol

关键词： adolescent alcohol astrocyte astrocyte morphology astrocyte-neuronal colocalization CA1 gabapentin GluA1 hippocampus tripartite synapse

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电学计量标准

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国外计量 1986年第2期 23-26页

作者： B.P.kibble 张钟华

一、前言电测仪表工业产品的分辨力和准确度一直在不断改进,自动化程度也不断提高。科学家们利用这些新式仪器可更深入地了解自然规律。为了保证这些不断改进的仪器的准确度,包括英国物理研究所(NPL)在内的国家计量实验室有义务用具体... 详细信息

一、前言电测仪表工业产品的分辨力和准确度一直在不断改进,自动化程度也不断提高。科学家们利用这些新式仪器可更深入地了解自然规律。为了保证这些不断改进的仪器的准确度,包括英国物理研究所(NPL)在内的国家计量实验室有义务用具体标准来实现电单位,并使它们具有比被测量更高的复现性。但是目前电单位还不能象米单位那样足够准确地从其定义

关键词：无定向保存单位计算电容器电学量单位基准器定义电学计量

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Breath Biopsy^(®) to Identify Exhaled Volatile Organic Compounds Biomarkers for Liver Cirrhosis Detection

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Journal of Clinical and Translational Hepatology 2023年第3期11卷 638-648页

作者： Giuseppe Ferrandino Giovanna De Palo Antonio Murgia Owen Birch Ahmed Tawfike Rob Smith Irene Debiram-Beecham Olga Gandelman Graham kibble Anne Marie Lydon Alice Groves Agnieszka Smolinska Max Allsworth Billy Boyle Marc P.van der Schee Michael Allison Rebecca C.Fitzgerald Matthew Hoare Victoria K.Snowdon Owlstone Medical CambridgeUK Department of Oncology University of CambridgeHutchison/MRC Research CentreCambridgeUK Department of Pharmacology and Toxicology School for Nutrition and Translational Research in Metabo-lism(NUTRIM)Maastricht University Medical Centerthe Netherlands Department of Medicine University of CambridgeAddenbrooke’s HospitalCambridgeUK Addenbrookes Hepatology and Liver Transplantation Unit Addenbrookes Hospi-talCambridgeUK MRC Cancer Unit Hutchison/MRC Research CentreUniversity of CambridgeCambridgeUK CRUK Cambridge Institute CambridgeUK

Background and Aims:The prevalence of chronic liver dis-ease in adults exceeds 30%in some countries and there is significant interest in developing tests and treatments to help control disease progression and reduce healthcare *** is a rich sampling matrix that offers non-invasive so-lutions suitable for early-stage detection and disease *** previously investigated targeted analysis of a single biomarker,here we investigated a multiparametric approach to breath testing that would provide more robust and reliable results for clinical ***:To identify can-didate biomarkers we compared 46 breath samples from cir-rhosis patients and 42 from *** and analysis used Breath Biopsy OMNI™,maximizing signal and contrast to background to provide high confidence biomarker detec-tion based upon gas chromatography mass spectrometry(GC-MS).Blank samples were also analyzed to provide de-tailed information on background volatile organic compounds(VOCs)***:A set of 29 breath VOCs differed significantly between cirrhosis and controls.A classification model based on these VOCs had an area under the curve(AUC)of 0.95±0.04 in cross-validated test *** seven best performing VOCs were sufficient to maximize classifica-tion performance.A subset of 11 VOCs was correlated with blood metrics of liver function(bilirubin,albumin,prothrom-bin time)and separated patients by cirrhosis severity using principal component ***:A set of seven VOCs consisting of previously reported and novel candidates show promise as a panel for liver disease detection and mon-itoring,showing correlation to disease severity and serum biomarkers at late stage.

关键词： Breath Biopsy Non-invasive Biomarker Cirrhosis Liver function test.

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老年股骨颈骨折半髋置换假体选择:2006~2018年卫生系统数据

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临床骨科杂志 2021年第5期24卷 663-663页

作者： kibble K PECK S C PARIKH H R 胡孔足(摘译) 不详

老年股骨颈骨折越来越多采用半髋置换治疗。2014年美国骨科医师协会指南推荐骨水泥型单动头假体。作者回顾性分析了2006~2018年卫生系统数据,3个治疗中心940例老年股骨颈骨折患者,平均年龄85岁,体重指数24 kg/m^(2)。33例90 d内死亡,45... 详细信息

老年股骨颈骨折越来越多采用半髋置换治疗。2014年美国骨科医师协会指南推荐骨水泥型单动头假体。作者回顾性分析了2006~2018年卫生系统数据,3个治疗中心940例老年股骨颈骨折患者,平均年龄85岁,体重指数24 kg/m^(2)。33例90 d内死亡,45例1年内死亡。病死率不同类型假体无差异。双动头生物型固定假体最贵(US$3900.61±US$2607.54),单动头骨水泥型假体最便宜(US$2618.68±US$1834.16)。

关键词：老年股骨颈骨折半髋置换骨科医师骨水泥型假体假体选择系统数据体重指数

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