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Vagus nerve stimulation is a potential treatment for ischemic stroke

Neural Regeneration Research 2023年第4期18卷 825-831页

作者： Yi-Lin Liu san-Rong Wang Jing-Xi Ma Le-Hua Yu gong-Wei Jia Department of Rehabilitation The Second Affiliated Hospital of Chongqing Medical UniversityChongqingChina The Second Clinical College Chongqing Medical UniversityChongqingChina Department of Neurology Chongqing General HospitalChongqingChina Chongqing Key Laboratory of Neurodegenerative Diseases ChongqingChina

Microglia are the brain’s primary innate immune cells,and they are activated and affect pro-inflammatory phenotype or regulatory phenotype after ischemic *** nerve stimulation was shown to activate microglial phenotypic changes and exhibit neuroprotective effects in ischemia/reperfusion *** this study,we established rat models of ischemic stroke by occlusion of the middle cerebral artery and performed vagus nerve stimulation 30 minutes after *** found that vagus nerve stimulation caused a shift from a pro-inflammatory phenotype to a regulatory phenotype in microglia in the ischemic *** nerve stimulation decreased the levels of pro-inflammatory phenotype markers inducible nitric oxide synthase and tumor necrosis factorαand increased the expression of regulatory phenotype markers arginase 1 and transforming growth factorβthrough activatingα7 nicotinic acetylcholine receptor ***,α7 nicotinic acetylcholine receptor blockade reduced the inhibition of Toll-like receptor 4/nuclear factor kappa-B pathwayassociated proteins,including Toll-like receptor 4,myeloid differentiation factor 88,I kappa B alpha,and phosphorylated-I kappa B alpha,and also weakened the neuroprotective effects of vagus nerve stimulation in ischemic *** nerve stimulation inhibited Toll-like receptor 4/nuclear factor kappa-B expression through activatingα7 nicotinic acetylcholine receptor and regulated microglial polarization after ischemic stroke,thereby playing a role in the treatment of ischemic *** from this study confirm the mechanism underlying vagus nerve stimulation against ischemic stroke.

关键词： cerebral ischemia microglia neuroprotection nuclear factor kappa-B pro-inflammatory phenotype regulatory phenotype reperfusion Toll-like receptor 4 vagus nerve stimulation α7 nicotinic acetylcholine receptor

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F-box only protein 2 exacerbates non-alcoholic fatty liver disease by targeting the hydroxyl CoA dehydrogenase alpha subunit

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World Journal of Gastroenterology 2023年第28期29卷 4433-4450页

作者： Zhi Liu Ning-Yuan Chen Zhao Zhang Sai Zhou san-Yuan Hu Department of General Surgery Qilu Hospital of Shandong UniversityJinan 250012Shandong ProvinceChina Department of General Surgery Shandong Provincial Qian Foshan HospitalShandong UniversityJinan 250014Shandong ProvinceChina Department of General Surgery Shandong First Medical University&Shandong Academy of Medical SciencesJinan 250117Shandong ProvinceChina

BACKGROUND Non-alcoholic fatty liver disease(NAFLD)is a major health burden with an increasing global ***,the unavailability of knowledge underlying NAFLD pathogenesis inhibits effective preventive and therapeutic *** To explore the molecular mechanism of *** Whole genome sequencing(WGS)analysis was performed on liver tissues from patients with NAFLD(n=6)and patients with normal metabolic conditions(n=6)to identify the target genes.A NAFLD C57BL6/J mouse model induced by 16 wk of high-fat diet feeding and a hepatocyte-specific F-box only protein 2(FBXO2)overexpression mouse model were used for in vivo *** transfection,co-immunoprecipitation-based mass spectrometry assays,and ubiquitination in HepG2 cells and HEK293T cells were used for in vitro *** A total of 30982 genes were detected in WGS analysis,with 649 up-regulated and 178 *** of FBXO2,an E3 ligase,was upregulated in the liver tissues of patients with ***-specific FBXO2 overexpression facilitated NAFLD-associated phenotypes in *** of FBXO2 aggravated odium oleate(OA)-induced lipid accumulation in HepG2 cells,resulting in an abnormal expression of genes related to lipid metabolism,such as fatty acid synthase,peroxisome proliferator-activated receptor alpha,and so *** contrast,knocking down FBXO2 in HepG2 cells significantly alleviated the OA-induced lipid accumulation and aberrant expression of lipid metabolism *** hydroxyl CoA dehydrogenase alpha subunit(HADHA),a protein involved in oxidative stress,was a target of FBXO2-mediated ***2 directly bound to HADHA and facilitated its proteasomal degradation in HepG2 and HEK293T *** with HADHA alleviated lipid accumulation caused by FBXO2 overexpression in HepG2 *** FBXO2 exacerbates lipid accumulation by targeting HADHA and is a potential therapeutic target for NAFLD。

关键词： F-box only protein 2 Nonalcoholic fatty liver disease The hydroxyl CoA dehydrogenase alpha subunit Liver steatosis Ubiquitination Lipid accumulation

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Asymmetric hydroazidation ofα-substituted vinyl ketones catalyzed by chiral primary amine

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Chinese Chemical Letters 2017年第5期28卷 1083-1086页

作者： Zai-Kun Xue Nian-Kai Fu san-Zhong Luo Beijing National Laboratory for Molecular Sciences(BNLMS) CAS Key Laboratory of Molecular Recognition and FunctionInstitute of ChemistryChinese Academy of SdencesBeijing 100190China

We report herein the first example of asymmetric hydroazidation of α-substituted vinyl ketones by using chiral primary amines as the catalysts.A simple chiral primary-tertiary diamine catalyst derived from lphenylalanine was found to readily promote this aza-Michael addition reaction with enamine protonation as the key stereogenic step,thus enabling the effective synthesis of α-chiral β-azido ketones with good yields and moderate enantioselectivities.

关键词： Chiral primary amine catalysis Hydroazidation Enamine protonation α-Substituted vinyl ketones Aza-Michael addition Chiral β-azido ketones

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Performances of Aluminum-cobalt Co-substituted α-Ni(OH)_2 Electrodes

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Chemical Research in Chinese Universities 2004年第3期20卷 350-353页

作者： ZHANGHeng-bin LIUHan-san CAOXue-jing SUNChia-chung CollegeofChemistry JilinUniversityChangchun130023P.R.China StateKeyLaboratoryofTheoreticalandComputationalChemistry InstituteofTheoreticalChemistryJilinUniversityChangchun130023P.R.China

Aluminum\|cobalt co\|substituted \%α\%\|Ni(OH)\-2 was prepared by means of the titration method in a buffer solution,the structure was characterized by XRD analysis. With above mentioned \%α\%\|Ni(OH)\-2 as the positive electrode of a nickel\|metal hydride cell,the discharge performances were examined by constant\|current charge\|discharge experiments. In comparison with the electrodes made of aluminum substituted or cobalt substituted Ni(OH)\-2 materials,the aluminum\|cobalt co\|substituted composite electrodes possess an excellent electrochemical performance and are of practical significance.

关键词： α-Ni(OH)2 Aluminum Cobalt Nickel-metal hydride cell

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Stable transfection of estrogen receptor-alpha suppresses expression of cyclooxygenase-2 and vascular endothelial growth factor-C in MDA-MB-231 breast cancer cells

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Chinese Medical Journal 2010年第15期123卷 1989-1994页

作者： ZHANG Hui LIN Ying XIAO Ying WANG san-ming LIU Xiang-xia WANG Shen-ming Division of Breast Surgery Department of Surgery the First Affiliated Hospital Sun Yat-sen University GuangzhouGuangdong 510080 China

Background Estrogen receptor （ER）-negative breast cancer cells are more aggressive than ER-positive cells. Elevated levels of cyclooxygenase-2 （COX-2） and vascular endothelial growth factor-C （VEGF-C） expression have been detected in cultured human breast cancer cells and are associated with negative hormone receptor status. In this study, we created ERα stable transfectants in MDA-MB-231 cells to explore the effect of ERα on cell growth and COX-2 and VEGF-C *** The green fluorescent protein （GFP）-ERα plasmids were stably transfected into ER-negative MDA-MB-231 cells. The proliferation and migration of untransfected MDA-MB-231 cells, ERα-transfected MDA-MB-231 cells and ER-positive MCF-7 cells were determined. The expression of COX-2, and the levels of VEGF-C mRNA and the VEGF-C secretion concentration were assayed in these cell *** The proliferation and migration capacities of ERα-tranfected MDA-MB-231 cells were significantly decreased （P 〈0.05）. The expression of COX-2 was significantly lower in ERa-tranfected MDA-MB-231 cells than in untranfected MDA-MB-231 cells. The mRNA and protein levels of VEGF-C were lower in ERa-tranfected MDA-MB-231 cells than in untransfected MDA-MB-231 cells （P〈0.05）.Conclusions ERα stable transfection inhibits proliferation and migration capacities of MDA-MB-231 cells and decreases expression of COX-2 and VEGF-C. The decreases of proliferation and migration capacities may be related to suppression of COX-2 and VEGF-C expression.

关键词： breast neoplasms estrogen receptor-alpha cyclooxygenase-2 vascular endothelial growth factor-C

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A Thermochemical Study on Complex Nickel(Ⅱ) L-α-Histidine

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Chemical Research in Chinese Universities 2003年第2期19卷 227-230页

作者： GAO Sheng li CHEN san ping HU Rong zu SHI Qi zhen Shaanxi Key Laboratory of Physico Inorganic Chemistry Department of ChemistryNorthwest UniversityXi′an 710069P.R.China

The formation enthalpy of complex nickel(Ⅱ) histidine(His) in water was determined by means of microcalorimetry in the temperature range of 298 15-323 15 K. The standard enthalpy of the formation of Ni(His) 2+ 2(aq) was calculated. On the basis of the experimental and the calculated results, three thermodynamic parameters(the activation enthalpy, the activation entropy and the activation free energy), the rate constants, three kinetic parameters(the apparent activation energy, the pre exponential constant and the reaction order) of the formation reaction of the title complex were obtained.

关键词： Nickel chloride L-α-Histidine Complex Formation reaction Thermokinetics

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Melatonin reduces acute lung injury in endotoxemic rats

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Chinese Medical Journal 2009年第12期122卷 1388-1393页

作者： SHANG You XU san-peng WU Yan JIANG Yuan-xu WU Zhou-yang YUAN Shi-ying YAO Shang-long Department of A-naesthesiology and Intensive Care Medicine Union nospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430022 China Department of Neurology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430022 China Department of Pathology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China

Background Treatment with melatonin significantly reduces lung injury induced by bleomycin, paraquat and ischemia reperfusion. In the present study, we investigated the possible protective roles of melatonin in pulmonary inflammation and lung injury during acute endotoxemia. Methods Thirty-two male Sprague-Dawley rats were randomly assigned to four groups： vehicle ＋ saline group, melatonin ＋ saline group, vehicle ＋ lipopolysaccharide group, melatonin ＋ lipopolysaccharide group. The rats were treated with melatonin （10 mg/kg, intraperitoneal injection （i.p.）） or vehicle （1% ethanol saline）, 30 minutes prior to lipopolysaccharide administration （6 mg/kg, intravenous injection）. Four hours after lipopolysaccharide injection, samples of pulmonary tissue were collected. Blood gas analysis was carried out. Optical microscopy was performed to examine pathological changes in lungs and lung injury score was assessed. Wet/dry ratios （W/D）, myeloperoxidase activity, malondialdehyde concentrations and tumor necrosis factor-alpha （TNF-α） and interleukin-10 （IL-10） levels in lungs were measured. The pulmonary expression of nuclear factor-kappa B （NF-κB） p65 was evaluated by Western blotting. Results PaO2 in the vehicle ＋ lipopolysaccharide group decreased compared with that in the vehicle ＋ saline group. This decrease was significantly reduced in the melatonin ＋ lipopolysaccharide group. The lung tissues from the saline ＋ lipopolysaccharide group were significantly damaged, which were less pronounced in the melatonin ＋ lipopolysaccharide group. The W/D ratio increased significantly in the vehicle ＋ lipopolysaccharide group （6.1±0.18） as compared with that in the vehicle ＋ saline group （3.61±0.3）（P 〈0.01）, which was significantly reduced in the melatonin ＋ lipopolysaccharide group （4.8±0.25）（P 〈0.01）. Myeloperoxidase activity and malondialdehyde levels increased significantly in the vehicle ＋ lipopolysaccharide group compared with that in the vehicle ＋ saline group, which was re

关键词： endotoxemia acute lung injuly melationin

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Valproic acid attenuates the multiple-organ dysfunction in a rat model of septic shock

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Chinese Medical Journal 2010年第19期123卷 2682-2687页

作者： SHANG You JIANG Yuan-xu Ding Ze-jun Shen Ai-ling XU san-peng YUAN Shi-ying YAO Shang-long Department of Anaesthesiology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430022 China Department of Critical Care Medicine Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430022 China Department of Pathology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China

Background Valproic acid （VPA） improves early survival and organ function in a highly lethal poly-trauma and hemorrhagic shock model or other severe insults. We assessed whether VPA could improve organ function in a rat model of septic shock and illustrated the possible mechanisms. Methods Forty Sprague-Dawley rats were randomly assigned to four groups （n=-10）： control group, VPA group, LPS group, and LPS＋VPA group. Lipopolysaccharide （LPS）（10 mg/kg） was injected intravenously to replicate the experimental model of septic shock. Rats were treated with VPA （300 mg/kg, i.v.） or saline. Six hours after LPS injection, blood was sampled for gas analysis, measurement of serum alanine aminotransferase, aspartate aminotransferase, urine nitrogen, creatinine and tumor necrosis factor-alpha. Lung, liver and kidney were collected for histopathological assessment. In addition, myeloperoxidase activity and tumor necrosis factor-α in pulmonary tissue were measured. Acetylation of histone H3 in lung was also evaluated by Western blotting. Results LPS resulted in a significant decrease in PaO2, which was increased by VPA administration followed LPS injection. In addition, LPS also induced an increase in the serum levels of alanine aminotransferase, aspartate aminotransferase, urine nitrogen, creatinine, and tumor necrosis factor-alpha. However, these increases were attenuated in the LPS＋VPA group. The lungs, liver and kidneys from the LPS group were significantly damaged compared with the control group. However, the damage was attenuated in the LPS＋VPA group. Myeloperoxidase activity and tumor necrosis factor-alpha levels in pulmonary tissue increased significantly in the LPS group compared with the control group. These increases were significantly inhibited in the LPS＋VPA group. Acetylation of histone H3 in lung tissue in the LPS group was inhibited compared with the control. However, the level of acetylation of histone H3 in the LPS＋VPA group was markedly elevated in contrast to

关键词： valproic acid septic shock inflammation acetylation

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Reduction of pulmonary inflammatory response by erythropoietin n a rat model of endotoxaemia

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Chinese Medical Journal 2009年第7期122卷 834-838页

作者： SHANG You JIANG Yuan-xu XU san-peng WU Yan WU Zhou-yang YUAN Shi-ying YAO Shang-long Department of Anesthesiology and Intensive Care Medicine Union Hospital Tongji Medical CollegeHuazhong Vniversiiy of Science and Technology Wuhan Hubei430022 China Department of Neurology Union Hospital Tongji Medical CollegeHuazhong Vniversiiy of Science and Technology Wuhan Hubei430022 China Department of Pathology Tongji Hospital Tongji Medical CollegeHuazhong University of Science and Technology Wuhan Hubei430030 China

Background Erythropoietin elicits protective effects in lung tissue injury induced by ischaemic reperfusion and hyperoxia. We investigated the protective roles of erythropoietin in pulmonary inflammation and lung injury during acute endotoxaemia.Methods A total of 32 male Sprague-Dawley rats were randomly assigned to four groups： saline group, erythropoietin＋saline group, saline＋lipopolysaccharide group and erythropoietin＋lipopolysaccharide group. Rats were treated with erythropoietin （3000 U/kg, i.p.） or saline, 30 minutes prior to lipopolysaccharide administration （6 mg/kg, i.v.）. Four hours after lipopolysaccharide injection, samples of pulmonary tissue were collected. Optical microscopy was performed to examine pathological changes in lungs. Wet/dry （W/D） ratios, myeloperoxidase activity, malondialdehyde concentrations and tumour necrosis factor-alpha （TNF-α） as well as interleukin 1 beta （IL-1β） levels in lungs were measured. The pulmonary expression of nuclear factor kappaB （NF-κB） p65 was evaluated by Western blotting. Differences between the different groups were analysed by one-way analysis of variance （ANOVA）.Results The lung tissues from the saline＋lipopolysaccharide group were significantly damaged, which were less pronounced in the erythropoietin＋lipopolysaccharide group. The W/D ratio increased significantly in the saline＋lipopolysaccharide group （5.75±0.22） as compared with the saline group （3.85±0.20）（P 〈0.01）, which was significantly reduced in the erythropoietin＋lipopolysaccharide group （4.50±0.35）（P 〈0.01）. Myeloperoxidase activity and malondialdehyde levels increased significantly in the saline＋lipopolysaccharide group compared with the saline group, which was reduced in the erythropoietin ＋ lipopolysaccharide group. The TNF-α level of pulmonary tissue increased significantly in the saline＋lipopolysaccharide group （（9.80±0.82） pg/mg protein） compared with the saline group （（4.20=L-0.42） pg/mg protein, P 〈0.01）. However, the increase of TNF-

关键词： endotoxaemia acute lung injury erythropoietin

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基于双动态头Sparse R-CNN的表面缺陷检测算法

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仪表技术与传感器 2023年第5期 97-105,111页

作者：郑亚睿蒋三新上海电力大学电子与信息工程学院上海201306

为了减少缺陷检测中的冗余检测,提出基于双动态头Sparse R-CNN的缺陷检测算法,2个动态头的责任不同:第1个负责不同尺度和空间的特征提取,第2个负责匹配可学习的提议特征。为了更好地提取图像细节信息,改进特征金字塔(FPN)为特征金字塔网... 详细信息

为了减少缺陷检测中的冗余检测,提出基于双动态头Sparse R-CNN的缺陷检测算法,2个动态头的责任不同:第1个负责不同尺度和空间的特征提取,第2个负责匹配可学习的提议特征。为了更好地提取图像细节信息,改进特征金字塔(FPN)为特征金字塔网格(FPG),并且与第1个动态头相结合进行特征提取。其次,提出了交流注意力来改进检测阶段的多头自注意力模块,减少随着迭代注意力图相似导致建模能力下降的问题。最后,改进边框回归损失函数GIoU为alpha-CIoU,加速收敛并提升检测的精度。实验结果表明:算法在晶圆和热轧钢2种表面缺陷数据集上都取得很好效果,平均精度分别为94.3%和88.1%。

关键词：表面缺陷检测动态头稀疏预测注意力机制标签匹配端到端预测

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