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NEW FOCUSED ULTRASOUND TRANSDUCER FOR FAT CELL DISRUPTION

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Journal of Innovative Optical Health Sciences 2012年第1期5卷 85-90页

作者： YANG CUI jue PENG hu TANG TIANFU WANG SIPING CHEN Department of Biomedical Engineering School of Medicine Shenzhen Key Laboratory of Biomedical Engineering Shenzhen UniversityShenzhen 518060 GuangdongChina

Being an emerging body-shaping technology of fat cell disruption,high-intensity focused ultrasound has been investigated intensively in recent years for its favorable natures such as painlessness,safety and *** of the major problems for the technology,however,is the overheating of *** this study,we modified the transducer design in order to solve the overheating *** simulated the performance of the modified design by finite element analysis and fabricated the newly designed *** measuring the actual performance data,we proved that the new design can effectively reduce temperature rise while keeping the acoustic intensity field unaffected.

关键词： Focused ultrasound transducer fat disruption acoustic field overheating

Cytogenetic analysis in two scallops (Bivalvia:Pectinidae) by PRINS and PI banding

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Acta Oceanologica Sinica 2007年第5期26卷 153-158页

作者： ZHANG Lingling BAO Zhenmin WANG jue WANG Shi huANG Xiaoting hu Xiaoli hu Jingjie Laboratory of Marine Genetics and Breeding College of Marine Life Sciences Ocean University of China Qingdao 266003 China

Cytogenetic analysis was performed for the bay scallop （Argopecten irradians Lamarck 1819） and the Japanese scallop （Patinopecten yessoensis Jay 1857 ） by primed in situ labeling （PRINS） and propidium iodide （PI） banding techniques. The PRINS analysis revealed that major rRNA genes were clustered in two loci on the telomeric regions of the short arms on two acrocentric chromosome pairs in A. irradians and on two submetacentric pairs in P. yessoensis. The histone H3 gene sites differed in number and location between these two species. The C-band-like patterns revealed by PI staining varied considerably between these two species. A. irradians displayed terminal bands at long arms on all chromosomes, centromeric bands on some pairs and interstitial bands on five pairs. P. yessoensis exhibited only centromeric bands on all chromosomes. These results would contribute to the better understanding of karyotype evolution in A. irradians and P. yessoensis.

关键词： primed in situ labeling PI banding Argopecten irradians Patinopecten yessoensis

基于网络药理学及分子对接探讨四逆散对溃疡性结肠炎与抑郁症“异病同治”的作用机制

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中药新药与临床药理 2024年第1期35卷 83-91页

作者：单佳铃胡伟琼谢勤白薇胡田彧吕燕妮江明金南昌大学第一附属医院江西南昌330006 复旦大学附属肿瘤医院闵行分院上海200240 江西省肿瘤医院江西南昌330029

目的基于网络药理学及分子对接探讨四逆散对溃疡性结肠炎与抑郁症“异病同治”的作用机制。方法利用TCMSP数据库获取四逆散的潜在活性成分及其相关作用靶点;利用GeneCards、CTD、TTD数据库筛选溃疡性结肠炎和抑郁症的疾病相关靶点;对... 详细信息

目的基于网络药理学及分子对接探讨四逆散对溃疡性结肠炎与抑郁症“异病同治”的作用机制。方法利用TCMSP数据库获取四逆散的潜在活性成分及其相关作用靶点;利用GeneCards、CTD、TTD数据库筛选溃疡性结肠炎和抑郁症的疾病相关靶点;对上述预测得到的活性成分作用靶点与疾病相关靶点取交集,获得四逆散治疗溃疡性结肠炎和抑郁症的潜在作用靶点(共有靶点),采用Cytoscape 3.7.2软件构建“中药-活性成分-疾病-共有靶点”网络,分析核心成分;将共有靶点导入STRING数据库,构建共有靶蛋白相互作用(PPI)网络,分析关键靶点;通过DAVID数据库对共有靶点进行GO功能及KEGG通路富集分析;对核心成分与关键靶点进行分子对接验证。结果共获得四逆散的活性成分136个,四逆散治疗溃疡性结肠炎和抑郁症的潜在作用靶点(共有靶点)220个,涉及657个生物过程、70个细胞组分、147个分子功能以及133条信号通路。筛选得到槲皮素、山柰酚、木犀草素、柚皮素、7-甲氧基-2-甲基异黄酮等核心活性化合物,JUN、MAPK3、STAT3、AKT1、MAPK1等关键靶蛋白,以及TNF、IL-17、Th17细胞分化、HIF-1、Toll样受体等信号通路。5个关键靶点均与槲皮素、山柰酚、木犀草素、柚皮素有较强的结合活性。结论四逆散可能是通过槲皮素、山柰酚、木犀草素、柚皮素等活性成分,作用于JUN、MAPK3、STAT3、AKT1、MAPK1等关键靶点,通过TNF、IL-17、HIF-1、Toll样受体、Th17细胞分化等信号通路,发挥对溃疡性结肠炎与抑郁症“异病同治”的作用。

关键词：四逆散溃疡性结肠炎抑郁症异病同治网络药理学分子对接作用机制

Effects and mechanisms of fluorite on the co-reduction of blast furnace dust and seaside titanomagnetite

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International Journal of Minerals,Metallurgy and Materials 2017年第11期24卷 1201-1210页

作者： Tian-yang hu Ti-chang Sun jue Kou Chao Geng Yong-qiang Zhao School of Civil and Resources Engineering University of Science and Technology Beijing Beijing 100083 China Key Laboratory of Ministry of Education of China for Efficient Mining and Safety of Metal Mines Beijing 100083 China

The co-reduction roasting and grinding-magnetic separation of seaside titanomagnetite and blast furnace dust was investigated with and without fluorite addition at a reduction roasting temperature of 1250°C for 60 min, a grinding fineness of-43 μm accounting for 69.02 wt% of the total, and a low-intensity magnetic field strength of 151 kA/m. The mineral composition, microstructure, and state of the roasted products were analyzed, and the concentrations of CO and CO_2 were analyzed in the co-reduction roasting. Better results were achieved with a small fluorite dosage(≤4 wt%) in the process of co-reduction. In addition, F^- was found to reduce the melting point and viscosity of the slag phase because of the high content of aluminate and silicate minerals in the blast furnace dust. The low moisture content of the blast furnace dust and calcic minerals inhibited the hydrolysis of CaF_2 and the loss of F^-. Compared with the blast furnace dust from Chengdeng, the blast furnace dusts from Jiugang and Jinxin inhibited the diffusion of F-when used as reducing agents, leading to weaker effects of fluorite.

关键词： seaside titanomagnetite blast furnace dust co-reduction roasting fluorite mechanisms

Clinical benefits of a modified Cryopiece system for cryopreservation of rare ejaculated and testicular spermatozoa for ICSI

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Asian Journal of Andrology 2022年第5期24卷 533-539页

作者： Wei Chen Chuan huang Peng Li Feng Liu Jian Sun Zi-jue Zhu Jing Zhai Yuan Xu Yan Hong Jian-Lin hu Yun-Peng Peng Zhen-Bo Zhang Yu Wu Zheng Li Department of ART Department of AndrologyCenter for Men’s HealthInstitute of Urologic Medical CenterShanghai General HospitalShanghai Key Lab of Reproductive MedicineShanghai Jiao Tong UniversityShanghai 200080China Clinical Research Center for Reproduction and Genetics in hunan Province Reproductive and Genetic Hospital of CITIC-XiangyaChangsha 410000China Department of Urology Affiliated Hospital of Xuzhou Medical UniversityXuzhou 221000China

Cryopreservation of rare testicular-retrieved spermatozoa for intracytoplasmic sperm injection(ICSI)in patients with severe oligozoospermia and azoospermia remains a major challenge in clinical *** study evaluated the Cryopiece system as a potential technique to cryopreserve rare human spermatozoa for *** numbers of ejaculated(24 patients)and testicular(13 patients)spermatozoa were cryopreserved using the Cryopiece *** total number of recovered spermatozoa and motility were assessed after ***-seven couples underwent ICSI using spermatozoa cryopreserved by the Cryopiece system,and ICSI outcomes(rates of fertilization,embryo cleavage,and clinical pregnancy)were *** average sperm post-thaw retrieval rate was 79.1%,and motility was 29.7%.Ejaculated spermatozoa had a higher post-thaw motility(32.5%)than testicular spermatozoa(21.8%;P=0.005).ICSI achieved a fertilization rate of 61.9%,embryo cleavage rate of 84.6%,and clinical pregnancy rate of 43.3%.The ICSI outcomes in the ejaculated and testicular frozen-thawed spermatozoa were *** oocyte activation(AOA)after ICSI with motile(72.1%)or immotile(71.9%)spermatozoa resulted in a significantly higher fertilization rate than that when using motile spermatozoa without AOA(52.0%;P=0.005).However,AOA did not enhance the clinical pregnancy rate(55.6%or 40.0%vs 35.3%;P=0.703).The Cryopiece system is simple and useful for the cryopreservation of small numbers of ejaculated or testicular spermatozoa for ICSI in patients with severe oligozoospermia or nonobstructive azoospermia.

关键词： Cryopiece system intracytoplasmic sperm injection outcome rare sperm cryopreservation

基于蛋白质组学探讨羟基-α-山椒素对糖尿病心肌病的作用机制

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中国药理学通报 2024年第3期40卷 537-544页

作者：王雪黄硕杨羚肖雯婧呼永河成都中医药大学临床医学院四川成都610072 中国人民解放军西部战区总医院四川成都610083

目的基于蛋白质组学检测技术,探讨羟基-α-山椒素(hydroxy-α-sanshool,san)治疗糖尿病心肌病(diabetic cardiomyopathy,DCM)的作用机制。方法高脂饲料喂养及腹腔注射链脲佐菌素(streptozotocin,STZ)联合使用建立DCM模型。分为对照组(CO... 详细信息

目的基于蛋白质组学检测技术,探讨羟基-α-山椒素(hydroxy-α-sanshool,san)治疗糖尿病心肌病(diabetic cardiomyopathy,DCM)的作用机制。方法高脂饲料喂养及腹腔注射链脲佐菌素(streptozotocin,STZ)联合使用建立DCM模型。分为对照组(CON组)、DCM组和san治疗组(DCM+san组)。小动物心脏超声及病理染色评价小鼠心脏功能及组织形态,蛋白组学技术推测san对DCM的作用机制,Western blot实验验证cAMP/PKA信号通路关键蛋白表达水平。结果心脏超声与病理染色显示san对DCM小鼠心脏有保护作用。DCM+san组与DCM组进行蛋白组学检测,共鉴定160个差异蛋白,127个蛋白表达上调,33个蛋白表达下调;GO二级功能注释显示细胞过程、分子结构和细胞结构等功能;KEGG富集分析显示cAMP信号通路富集最为丰富;蛋白互作网络显示以PKA为中心节点与多种蛋白在cAMP信号通路中有互作关系。Western blot验证DCM组的cAMP和PKA蛋白相对表达量均明显低于CON组(Psan组的cAMP和PKA蛋白相对表达量均明显高于DCM组(Psan对糖尿病心功能有保护作用,通过cAMP信号通路发挥作用。

关键词：蜀椒羟基-α-山椒素蛋白组学糖尿病心肌病作用机制 cAMP信号通路

病毒诱发哮喘关键miRNA靶基因的预测与验证及潜在中药干预

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现代生物医学进展 2024年第6期24卷 1019-1029,1076页

作者：周智姚冰王孟清罗银河胡燕丁伊邓羿駃刘靖雷马啸湖南中医药大学湖南长沙410208 长沙市妇幼保健院湖南长沙410007 湖南中医药大学第一附属医院湖南中医药大学中医学国内一流建设学科湖南长沙410021 长沙市民政职业技术学院湖南长沙410018

目的:探究病毒诱发哮喘关键微小RNA(mi RNA)以及差异基因在哮喘发病中的调控作用,并寻找潜在的治疗靶点以及对应中药,为病毒诱发哮喘中医药辨证论治、早期截断哮喘的发生、传变提供诊疗思路。方法:首先通过查阅有关mi RNA与哮喘关系的文... 详细信息

目的:探究病毒诱发哮喘关键微小RNA(mi RNA)以及差异基因在哮喘发病中的调控作用,并寻找潜在的治疗靶点以及对应中药,为病毒诱发哮喘中医药辨证论治、早期截断哮喘的发生、传变提供诊疗思路。方法:首先通过查阅有关mi RNA与哮喘关系的文献,根据文献的影响因子、报道次数及与呼吸道合胞病毒(Respiratory syncytial virus,RSV)、树突细胞、自噬等病毒诱发哮喘发病机制的密切程度进行积分统计,按得分高低筛选出6个候选mi RNA:mi RNA-155、mi RNA-21、Let-7、mi RNA-34a、mi RNA-145、mi RNA-146a。然后通过靶基因数据库分析其功能,并通过生物信息学方法筛选出与关键目标mi RNA相关的信号通路。最后通过Coremine数据库查找核心基因对应中药,使用TCMSP、BATMAN-TCM数据库查找中药有效成分,利用Cytoscape软件进行可视化分析,并进行基因本体论(Gene ontology,GO)富集分析与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析。然后通过RSV联合鸡卵清蛋白诱导病毒诱发哮喘模型,并通过实时荧光定量PCR(qRT-PCR)法检测生物信息学预测的核心mi RNA表达水平。结果:体内实验验证结果显示:RSV诱导哮喘小鼠模型中气道上皮mi RNA-21、Let-7、mi RNA-155、mi RNA-146a表达量显著降低,提示这四种mi RNA可作为病毒诱发哮喘关键生物标志物;结果中共筛选出与病毒诱发哮喘最相关的10个核心靶基因,分别是:STK4、CTH、MRM3、EEF1A1、PDCD6IP、MARCKS、SLC25A5、GNAS、ALDOA、CFL1,也是治疗的关键靶点;一共包含对应中药共160多种,其中理气活血类药物、补虚药出现频次最高,具体的高频中药为:枳实、枳壳、香橼、油柑子、人参、人参叶、姜黄、郁金、丁香、水牛角。结论:Mi RNA能够通过靶向调控多种靶基因的表达,参与病毒疾病的发生、发展过程。RSV诱导哮喘气管组织中的mi RNA-21、Let-7、mi RNA-155、mi RNA-146a均有显著下调,可作为病毒诱发哮喘关键生物标志物,也是治疗的关键靶点,同时能够从mi RNA这一角度明确在病毒诱发哮喘中的中医药作用机制,可通过辨证论治适当使用理气活血类、补虚药等药物,以早期截断哮喘的病理进程。

关键词：儿童哮喘呼吸道合胞病毒靶基因生物信息学中医药数据挖掘

DEVELOPMENT OF EXPERIMENTAL METHODS FOR IMPACT TESTING BY COMBINING HOPKINSON PRESSURE BAR WITH OTHER TECHNIQUES

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Acta Mechanica Solida Sinica 2014年第4期27卷 331-344页

作者： Lili Wang Shisheng hu Liming Yang Zijian Sun jue Zhu huawei Lai Yuanyuan Ding Mechanics and Material Science Research Center Ningbo University Department of Modern Mechanics University of Science & Technology of China huafeng Faculty Ningbo Polytechnic Department of Municipal Engineering Zhejiang University of Water Resources and Electric Power

The split Hopkinson pressure bar（SHPB） technique and the wave propagation inverse analysis（WPIA） technique are both extensively used to experimentally investigate the impact behavior of materials, although neither of them alone provides a fully satisfactory analysis. In the present paper, attention is given to new experimental techniques by incorporating a damagemodified constitutive model into the SHPB technique and combining the Hopkinson pressure bar（HPB） technique with WPIA. First, to distinguish the response due to dynamic constitutive behavior and the response due to dynamic damage evolution, the SHPB method incorporating a damage-modified constitutive model is developed, including an explicit damage-modified Zhu–Wang–Tang model and an implicit damage-modified constitutive model. Second, when the SHPB results become invalid, a method of combining new Lagrange inverse analyses with the HPB technique is developed, including cases of the HPB arranged in front of a long specimen and behind the specimen. As examples of these new methods, typical results are given for nonlinear viscoelastic polymers and concretes considering damage evolution, a super-elastic Ti–Ni alloy with phase transformation and an aluminum foam with shock waves propagating within it.

关键词： Hopkinson pressure bar SHPB Lagrangian analysis high strain rate rate-dependent constitutive relation damage evolution

First scanning Fabry–Perot interferometer developed in China

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Chinese Science Bulletin 2014年第5期59卷 563-570页

作者： Guoyuan hu Yong Ai Yange Zhang Hong Zhang jue Liu School of Electronic Information Wuhan University

A scanning Fabry–Perot interferometer(SFPI)was first developed and deployed at the Langfang near Space Environment Field Scientific Observation Station(39.38°N,116.65°E)of the National Space Science Center,*** instrument is designed to measure the mesospheric and thermospheric wind velocities using the atomic oxygen 557.7-nm and 630.0-nm *** from February 28 to March 3and February 28 to March 15 in 2011 were chosen for case study and mean value study,*** errors of the meridional and zonal winds are 6.5 and 7.5 m/s at 557.7-nm and at 630.0-nm,they are 7.1 and 6.6 m/s,*** the whole experiment,the instrument has performed in good condition and provided high-quality *** mean neutral wind data were consistent with that predicted by *** agreement has been found in between the SFPI and a neighbor Meridian Project Fabry–Perot interferometer(MP FPI),with a corresponding coefficient(r2)larger than 80%.In general,the scanning FPI meets the design goal,and it is a useful ground-based instrument for measuring mesospheric and thermospheric winds at middle latitudes and is able to provide high-quality data for future scientific studies.

关键词：中国科学院扫描法干涉仪地面仪器空间环境科学中心大气风速设计目标