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Molecular cloning and characterization of three phenylalanine ammonia-lyase genes from Schisandra chinensis

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Chinese Journal of Natural Medicines 2022年第7期20卷 527-536页

作者： FAN san-Peng CHEN Wei WEI Jiang-Chun GAO Xiao-Xu YANG Yong-Cheng WANG An-hua hu Gao-Sheng JIA Jing-Ming School of Traditional Chinese Materia Medica Shenyang Pharmaceutical UniversityShenyang 110016China

Phenylalanine ammonia-lyase(PAL),which catalyzes the conversion from L-phenylalanine to trans-cinnamic acid,is a well-known key enzyme and a connecting step between primary and secondary metabolisms in the phenylpropanoid biosynthetic pathway of plants and *** chinensis,a woody vine plant belonging to the family of Magnoliaceae,is a rich source of dibenzocyclooctadiene lignans exhibiting potent ***,the functional role of PAL in the biosynthesis of lignan is relatively limited,compared with those in lignin and flavonoids ***,it is essential to clone and characterize the PAL genes from this valuable medicinal *** this study,molecular cloning and characterization of three PAL genes(ScPAL1−3)from *** was carried *** were cloned using RACE *** sequence analysis of the three ScPALs was carried out to give basic characteristics followed by docking *** order to determine their catalytic activity,recombinant protein was obtained by heterologous expression in pCold-TF vector in Escherichia coli(BL21-DE3),followed by Ni-affinity *** catalytic product of the purified recombinant proteins was verified using RP-HPLC through comparing with standard *** optimal temperature,pH value and effects of different metal ions were ***,Kcat and Km values were determined under the optimal *** expression of three ScPALs in different tissues was also *** work provided essential information for the function of ScPALs.

关键词： Schisandra chinensis Phenylalanine ammonia-lyase Molecular cloning and characterization Kinetics

Minimizing makespan in a two-stage hybrid flow shop scheduling problem with open shop in one stage

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Applied Mathematics(A Journal of Chinese Universities) 2013年第3期28卷 358-368页

作者： DONG Jian-ming hu jue-liang CHEN Yong Department of Mathematics Zhejiang Sci-Tech University Institute of Operational Research and Cybernetics Hangzhou Dianzi University

This paper considers a scheduling problem in two-stage hybrid flow shop, where the first stage consists of two machines formed an open shop and the other stage has only one machine. The objective is to minimize the makespan, i.e., the maximum completion time of all jobs. We first show the problem is NP-hard in the strong sense, then we present two heuristics to solve the problem. Computational experiments show that the combined algorithm of the two heuristics performs well on randomly generated problem instances.

关键词： hybrid flow shop open shop Heuristic algorithm.

DNA Nanotechnology-Enabled Fabrication of Metal Nanomorphology

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Research 2022年第4期2022卷 189-204页

作者： Mo Xie Yang hu jue Yin Ziwei Zhao Jing Chen Jie Chao State Key Laboratory of Organic Electronics and Information Displays e Jiangsu Key Laboratory for Biosensors Institute of Advanced Materials(IAM)Jiangsu National Synergetic Innovation Center for Advanced Materials(SICAM)Nanjing University of Posts and Telecommunications9 Wenyuan RoadNanjing 210023China The Interdisciplinary Research Center Shanghai Synchrotron Radiation FacilityZhangjiang LaboratoryShanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201210China

In recent decades,DNA nanotechnology has grown into a highly innovative and widely established *** nanostructures have extraordinary structural programmability and can accurately organize nanoscale materials,especially in guiding the synthesis of metal nanomaterials,which have unique advantages in controlling the growth morphology of metal *** review started with the evolution in DNA nanotechnology and the types of DNA ***,a DNA-based nanofabrication technology,DNA metallization,was *** this section,we systematically summarized the DNA-oriented synthesis of metal nanostructures with different morphologies and ***,the applications of metal nanostructures constructed from DNA templates in various fields including electronics,catalysis,sensing,and bioimaging were figured ***,the development prospects and challenges of metal nanostructures formed under the morphology control by DNA nanotechnology were discussed.

关键词： materials template synthesis

齐大山铁矿破碎流程能耗分布规律与模拟优化研究

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矿山机械 2024年第4期52卷 33-39页

作者：胡振涛王培龙祝焕威林嘉威刘政宇寇珏孙春宝鞍钢集团矿业有限公司齐大山分公司辽宁鞍山114043 北京科技大学土木与资源工程学院北京100083

针对某铁矿选矿厂三段一闭路旧系统存在的细碎作业负荷较大,能耗占比较高等问题,对破碎流程进行模拟优化。基于Moly-Cop Tools软件,进行了设备工艺模拟研究。结果表明,通过减小中碎破碎机紧边排料口宽度,可以有效提升中碎破碎机效率并... 详细信息

针对某铁矿选矿厂三段一闭路旧系统存在的细碎作业负荷较大,能耗占比较高等问题,对破碎流程进行模拟优化。基于Moly-Cop Tools软件,进行了设备工艺模拟研究。结果表明,通过减小中碎破碎机紧边排料口宽度,可以有效提升中碎破碎机效率并降低细碎作业负荷,提高旧系统矿石破碎流程的整体能量利用效率。

关键词：破碎流程能耗分布 Moly-Cop Tools 模拟优化

基于GAMLSS方法构建新疆维吾尔自治区部分地区10~13岁儿童生长曲线

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中国健康教育 2024年第3期40卷 199-204页

作者：热沙来提·阿不来提玛依拉·胡达拜尔地王倩白天雅祖丽皮耶·艾散肖辉新疆医科大学公共卫生学院新疆乌鲁木齐830011 新疆维吾尔自治区卫生健康宣传教育中心新疆乌鲁木齐830002

目的确定新疆维吾尔自治区部分地区儿童体格检测指标的百分位参考值和生长曲线,为新疆维吾尔自治区部分地区儿童生长发育评价和肥胖的识别提供参考依据。方法利用分层随机整群抽样方法对2327名10~13岁儿童进行了一项体格测量的横断面... 详细信息

目的确定新疆维吾尔自治区部分地区儿童体格检测指标的百分位参考值和生长曲线,为新疆维吾尔自治区部分地区儿童生长发育评价和肥胖的识别提供参考依据。方法利用分层随机整群抽样方法对2327名10~13岁儿童进行了一项体格测量的横断面研究。采用位置、尺度和形状的广义加性模型(GAMLSS)构建不同体格指标生长曲线。以BMI>P95,TMI> P95,腰围>P90,腰高比≥0.5设置为肥胖的阈值,评价儿童肥胖。BMI和腰围临界值与国家学龄儿童青少年肥胖筛查标准进行比较。结果男女生身高、体重、臀围随年龄增加而增加,TMI和腰高比随年龄增加而下降。10~13岁儿童使用BMI、TMI、腰围、腰高比的肥胖检出率分别为男生:7.8%、7.2%、12.4%和16.9%,女生:7.2%、6.7%、11.5%和8.8%。男生:BMI肥胖临界值10岁阶段高于国家筛查标准,11~13岁低于国家筛查标准,腰围肥胖临界值均高于国家筛查标准;女生:BMI肥胖临界值均低于国家筛查标准,腰围肥胖临界值10~12岁高于国家筛查标准,13岁阶段低于国家筛查标准。结论新疆部分地区学龄儿童以腰围判断肥胖的临界值高于国家标准,建议针对儿童肥胖的预防与管理中加强对腰围的关注。

关键词：儿童生长曲线肥胖横断面研究

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中西医结合学科发展关键问题识别研究

中华医学教育杂志 2024年第5期44卷 330-334页

作者：陈红岑珏高翔王道珍舒静晋永胡鸿毅施建蓉上海市胸科医院后勤保障部上海200030 上海交通大学医学院附属第六人民医院上海200233 上海交通大学医学院公共卫生学院社会医学与卫生事业管理系上海200025 上海交通大学医学院附属第六人民医院教学办公室上海200233 上海中医药大学上海201203 上海中医药大学教务处上海201203 上海市卫生健康委员会上海200125 上海交通大学医学院上海200025

目的分析我国中西医结合学科发展的关键问题,为促进中西医结合学科发展提供参考。方法以“中西医结合+学科发展/学科建设/教学”为主题词,在中国知网、万方数据知识服务平台、维普中文科技期刊数据库中检索2023年3月1日前公开发表的文献... 详细信息

目的分析我国中西医结合学科发展的关键问题,为促进中西医结合学科发展提供参考。方法以“中西医结合+学科发展/学科建设/教学”为主题词,在中国知网、万方数据知识服务平台、维普中文科技期刊数据库中检索2023年3月1日前公开发表的文献,通过文献评阅和专家咨询建立问题清单,计算各个问题的重要性指数、严重性指数和优先解决指数,并以此确认关键问题。结果共检索到文献43篇,通过分析归纳出我国中西医结合学科发展领域的主要问题14个,来自5家单位的6名中西医结合专家参与了咨询,其中优先解决指数排名前3位的分别为中西医结合住院医师规范化培训标准缺失、中西医结合教学模式不够成熟和中西医结合临床实践教学基地不足,优先解决指数分别为0.1518、0.1181和0.1104。结论中西医结合学科发展面临多方面的问题,而适宜的住院医师规范化培训标准、教学模式和临床实践教学基地是中西医结合学科发展的关键问题,建议完善中西医结合住院医师规范化培训标准、优化中西医结合教学模式和加强中西医结合临床实践教学基地建设,助力中西医结合学科发展。

关键词：中西医结合学科发展关键问题住院医师规范化培训文献分析

Application of "Fabulous" stent system to improve aortic remodeling after TEVAR for type B aortic dissection

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Chinese Medical Journal 2023年第10期136卷 1231-1233页

作者： Chengkai hu jue Yang Wei Wang Xiangchen Dai Xinwu Lu Youfei Qi Hongpeng Zhang Yuchong Zhang Ye Yuan Enci Wang Yi Si Weiguo Fu Lixin Wang Department of Vascular Surgery Zhongshan HospitalFudan UniversityShanghai 200032China Vascular Surgery Institute of Fudan University Shanghai 200032China Department of Vascular Surgery Zhongshan Hospital(Xiamen)Fudan UniversityXiamenFujian 361015China Department of Vascular Surgery Xiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Vascular Surgery Tianjin Medical University General HospitalTianjin 300052China Department of Vascular Surgery Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China Department of Vascular Surgery Hainan General HospitalHaikouHainan 570311China Department of Vascular Surgery Chinese PLA General HospitalBeijing 100853China

It is known that the Proximal Extension To Induce Complete Attachment(PETTICOAT)technique improves the distal aortic remodeling compared with standard thoracic endovascular aortic repair(TEVAR).[1,2]However,the only available Zenith Dissection Endovascular System(Cook Medical Inc.,Bloomington,Ind,USA)has several limitations,including only two sizes(36 mm and 46 mm)and an one-taper design,which may not be suitable for the complex morphology of dissection at distal aorta,particular for the east Asian ***,our center designed the new“Fabulous”stent system and acheieved better early curative effect.

关键词： aortic dissection remodeling

龙眼叶黄酮类有效组分配伍体外降糖作用的研究

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广西科学院学报 2024年第2期40卷 135-144页

作者：梁晶春胡珏安施佳郑飘雪陆春莲梁炎丽陈明伟梁洁广西中医药大学药学院广西南宁530200 广西高校中药提取纯化与质量分析重点实验室广西南宁530200 广西壮瑶药重点实验室广西南宁530200

基于体外降糖活性优选龙眼(Dimocarpus longan Lour.)叶黄酮有效成分山柰酚、槲皮素、槲皮苷的配伍比例。建立HepG2细胞胰岛素抵抗模型,分别考察不同浓度的胰岛素及不同诱导时间对胰岛素抵抗模型的诱导情况,并以山柰酚、槲皮素、槲皮苷... 详细信息

基于体外降糖活性优选龙眼(Dimocarpus longan Lour.)叶黄酮有效成分山柰酚、槲皮素、槲皮苷的配伍比例。建立HepG2细胞胰岛素抵抗模型,分别考察不同浓度的胰岛素及不同诱导时间对胰岛素抵抗模型的诱导情况,并以山柰酚、槲皮素、槲皮苷三者用量为考察因素,以对胰岛素抵抗的HepG2细胞葡萄糖消耗率为指标,运用星点设计-效应面法(CCD-RSM)优化三者配伍比例。结果表明,建立HepG2细胞胰岛素抵抗模型的最佳条件为1×10^(-7)mol/L胰岛素诱导24 h;山柰酚、槲皮素、槲皮苷体外降糖最佳配伍比例为山柰酚105.0μmol/L、槲皮素78.9μmol/L、槲皮苷194.0μmol/L,配伍比例优化试验拟合方程为Y=20.94+1.55×A-0.44×B-2.05×C-0.66×AB+1.68×AC-1.53×A^(2)-3.2×B^(2)-2.68×C^(2)。山柰酚、槲皮素、槲皮苷3种单体的联合使用对HepG2细胞的葡萄糖消耗率预测值是21.52%,实验值是(21.05±0.52)%。优选出的最佳配伍比例的龙眼叶黄酮类有效组分,对HepG2细胞的葡萄糖消耗率最高,降糖活性较好,可为龙眼叶治疗糖尿病的有效性提供科学方法与实验依据。

关键词：龙眼叶组分配伍山柰酚槲皮素槲皮苷

A Chromosome-level Genome Assembly of Wild Castor Provides New Insights into Its Adaptive Evolution in Tropical Desert

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Genomics, Proteomics & Bioinformatics 2022年第1期20卷 42-59页

作者： Jianjun Lu Cheng Pan Wei Fan Wanfei Liu huayan Zhao Donghai Li Sen Wang Lianlian hu Bing He Kun Qian Rui Qin jue Ruan Qiang Lin Shiyou Lü Peng Cui CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenChinese Academy of SciencesWuhan 430074China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China University of Chinese Academy of Sciences Beijing 100049China Sino-Africa Joint Research Center Chinese Academy of SciencesWuhan 430074China State Key Laboratory of Biocatalysis and Enzyme Engineering School of Life SciencesHubei UniversityWuhan 434200China

Wild castor grows in the high-altitude tropical desert of the African Plateau,a region known for high ultraviolet radiation,strong light,and extremely dry *** investigate the potential genetic basis of adaptation to both highland and tropical deserts,we generated a chromosome-level genome sequence assembly of the wild castor accession WT05,with a genome size of 316 Mb,a scaffold N50 of 31.93 Mb,and a contig N50 of 8.96 Mb,*** with cultivated castor and other Euphorbiaceae species,the wild castor exhibits positive selection and gene family expansion for genes involved in DNA repair,photosynthesis,and abiotic stress *** variations associated with positive selection were identified in several key genes,such as LIG1,DDB2,and RECG1,involved in nucleotide excision ***,a study of genomic diversity among wild and cultivated accessions revealed genomic regions containing selection signatures associated with the adaptation to extreme *** identification of the genes and alleles with selection signatures provides insights into the genetic mechanisms underlying the adaptation of wild castor to the high-altitude tropical desert and would facilitate direct improvement of modern castor varieties.

关键词： Ricinus communis L. Adaptive evolution Selection signature Genetic variation Genome assembly