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Molecular epidemiology, characterization of virulence factors and antibiotic resistance profile of Streptococcus agalactiae isolated from dairy farms in China and Pakistan

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Journal of Integrative Agriculture 2023年第5期22卷 1514-1528页

作者： Ambreen LEGHARI Shakeel Ahmed LAKHO Faiz Muhammad KHAND Khaliq ur Rehman BHUTTO sameen qayoom lone Muhammad Tahir ALEEM Iqra BANO Muhammad Ali CHANDIO Jan Muhammad SHAH LIN Hui-xing FAN Hong-jie MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary MedicineNanjing Agricultural UniversityNanjing 210095P.R.China Shaheed Benazir Bhutto University of Veterinary and Animal Sciences Sakrand 67210Pakistan Central Veterinary Diagnostic Laboratory Tando Jam 70050Pakistan Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou UniversityYangzhou 225009P.R.China

Streptococcus agalactiae is one of the most common pathogens that cause bovine mastitis worldwide. Identifying pathogen prevalence and virulence factors is critical for developing prevention and control approaches. Herein, 1 161 milk samples from various dairy farms in China(n=558) and Pakistan(n=603) were collected between 2019–2021 and were subjected to S. agalactiae isolation. Prevalence, serotyping, virulence genes, and antibiotic-resistant genes of S. agalactiae were evaluated by PCR assay. All isolates were characterized for haemolysis, biofilm production, cytotoxicity, adhesion, and invasion on bovine mammary epithelial cells. The prevalence of S. agalactiae-induced mastitis in cattle was found to be considerably higher in Pakistan than in China. Jiangsu and Sindh provinces had the highest area-wise prevalence in China and Pakistan, respectively. Serotypes Ia and II were prevalent in both countries, whereas serotype III was found only in Pakistan. Moreover, all isolates tested positive for PI-2b gene but negative for PI-1 and PI-2a genes. All isolates harboured cfb, cylE, hylB, and fbsB virulent genes, whereas many of them lacked bibA, rib and bca. However, the absence of bac and scp genes in Chinese isolates and cspA in Pakistani isolates was noted, while spb1 and lmb were not detected in isolates of both countries. Pakistani isolates, particularly serotype Iapositive, had a considerably higher ability to produce biofilm, haemolysis, cytotoxicity, adhesion, and invasion than Chinese isolates. Most of the isolates were phenotypically resistant to tetracycline, erythromycin, and clindamycin and genotypic resistance was confirmed by the presence of ermA, ermB, tetM and tetO genes. Our study highlights the antimicrobial resistance profile and virulence-related factors contributing to the epidemiological spread of mastitis-causing S. agalactiae in China and Pakistan. The findings may facilitate future studies designed to develop improved treatment and control str

关键词： Streptococcus agalactiae mastitis epidemiology virulence characterization antibiotic resistance

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Does strict environmental regulation enhance the global value chains position of China's industrial sector?

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Petroleum Science 2021年第6期18卷 1899-1909页

作者： Jin-Chao Wang Zhi-Da Jin Mian Yang sameen Naqvi Center for Economic Development Research Wuhan UniversityWuhan430072China Economics and Management School Wuhan UniversityWuhan430072China

Given the vital importance of global value chains(GVCs)position for a country's international competitiveness,this paper tries to investigate the impacts of environmental regulation on the GVCs position of China's industrial *** the latest value-added decomposition method,we first measure the GVCs position of China's industrial sector from 2003 to ***,both two-stage least squares(2 SLS)method with panel data and mediating effect model are employed to empirically examine the effects of environmental regulation on China's position in *** results indicate that environmental regulation has significantly upgraded the GVCs position of China's industrial sector,and the effect is more evident for the sub-sectors with originally lower GVCs *** mediation effect test shows that increasing R&D investment is an important channel through which environmental regulation affects the GVCs position of China's industrial sector,which verifies the existence of the Porter *** analysis finds that the enhancement of GVCs position of China's industrial sector caused by environmental regulation is mainly achieved through reducing the backward GVCs position.

关键词： Environmental regulation Global value chains Porter hypothesis Industrial sector

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Effects of Microencapsulation of Nisin and Isolated Antimicrobial Peptides Using Water/oil/water Emulsions on Preservation of Fresh Sichuan Pepper(Zanthoxylum Bungeanum)

Effects of Microencapsulation of Nisin and Isolated Antimicr...

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作者： Dur e sameen 四川农业大学

学位级别：硕士

To improve the shelf life of food,research for the betterment of food packaging materials is ongoing,and biobased and natural polymers are getting increasing *** quality packaging not only improves the shelf life of food but also prevents food wastage and *** plastics are not biodegradable,they tend to harm the environment in many ways;the marine environment and landfills have become major *** 14%of plastics are recycled and only 5%are replaced,while the remaining are a threat to the ***,research is being focused on better alternatives of food packaging ***,biodegradable,biocompatible,and environmentally-friendly packaging materials are gaining much more *** can be produced from natural resources and waste material of natural food and *** the present study,edible microcapsules coating packaging,made from natural sources,were used to improve the shelf life of fresh Zanthoxylum bungeanum（Sichuan pepper,Huajiao）.LS-MS and HPLC of extracted proteins evaluated total 18 peptides in sample and 16 variant protein IDs were found with specific molecular *** average molecular weight（M_n）of extracted protein was 1.613×103 g/mol,weight-average molecular weight（M）was 4.047×103 g/mol,higher-average molecular weight（M）was1.423×103 g/mol,and its highest-peak molecular weight was（M）1.939×103 g/*** polydispersity index（M/M_n）was also evaluated to be *** DSC,the endothermic peak between the two major peaks（158°C）shows the denaturation of isolated protein *** FTIR spectrum indicates the distribution of its absorption peaks from 500-4000 cm.3100-3700 cmis attributed to the basic stretching mode of the-OH group,while 3311 cmis the O-H stretching vibration.2800-3000 cmcorresponds to the C-H tensile vibration of the triglyceride carbonyl *** the absorption peak at 2992 cmis related to the tensile shock of the C-H（CH2）group of few fatty acids in isolated *** efficienc

关键词： Microencapsulation Edible coating Antimicrobial peptides Zanthoxylum bungeanum

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Improving Routine Immunization Coverage Through Optimally Designed Predictive Models

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Computers, Materials & Continua 2022年第1期70卷 375-395页

作者： Fareeha sameen Abdul Momin Kazi Majida Kazmi Munir A Abbasi Saad Ahmed Qazi Lampros K Stergioulas Faculty of Electrical and Computer Engineering NED University of Engineering and TechnologyKarachi75270Pakistan Department of Pediatrics and Child Health Aga Khan UniversityKarachi74800Pakistan Surrey Business School University of SurreyGuildfordGU27XHUnited Kingdom Neurocomputation Lab National Centre of Artificial IntelligenceNED University of Engineering&TechnologyKarachi75270Pakistan Faculty of IT and Design The Hague University of Applied Sciences2521 ENThe HagueThe Netherlands

Routine immunization(RI)of children is the most effective and timely public health intervention for decreasing child mortality rates around the *** being a low-and-middle-income-country(LMIC)has one of the highest child mortality rates in the world occurring mainly due to vaccine-preventable diseases(VPDs).For improving RI coverage,a critical need is to establish potential RI defaulters at an early stage,so that appropriate interventions can be targeted towards such populationwho are identified to be at risk of missing on their scheduled vaccine *** this paper,a machine learning(ML)based predictivemodel has been proposed to predict defaulting and non-defaulting children on upcoming immunization visits and examine the effect of its underlying contributing *** predictivemodel uses data obtained from Paigham-e-Sehat study having immunization records of 3,113 *** design of predictive model is based on obtaining optimal results across accuracy,specificity,and sensitivity,to ensure model outcomes remain practically relevant to the problem *** optimization of predictive model is obtained through selection of significant features and removing data *** machine learning algorithms were applied for prediction of defaulting children for the next immunization *** results showed that the random forest model achieves the optimal accuracy of 81.9%with 83.6%sensitivity and 80.3%*** main determinants of vaccination coverage were found to be vaccine coverage at birth,parental education,and socioeconomic conditions of the defaulting *** information can assist relevant policy makers to take proactive and effective measures for developing evidence based targeted and timely interventions for defaulting children.

关键词： Machine learning predictive models routine immunization vaccine coverage pakistan optimization SMOTE

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DISTINIT:Data poISoning atTacks dectectIon usiNg optIized jaCcard disTance

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Computers, Materials & Continua 2022年第12期 4559-4576页

作者： Maria sameen Seong Oun Hwang Department of IT Convergence Engineering Gachon UniversitySeongnam-si13120Korea Department of Computer Engineering Gachon UniversitySeongnam-si13120Korea

Machine Learning(ML)systems often involve a re-training process to make better predictions and *** re-training process creates a loophole and poses a security threat for ML *** leverage this loophole and design data poisoning attacks against ML *** poisoning attacks are a type of attack in which an adversary manipulates the training dataset to degrade the ML system’s *** poisoning attacks are challenging to detect,and even more difficult to respond to,particularly in the Internet of Things(IoT)*** address this problem,we proposed DISTINIT,the first proactive data poisoning attack detection framework using *** found that Jaccard Distance(JD)can be used in the DISTINIT(among other distance measures)and we finally improved the JD to attain an Optimized JD(OJD)with lower time and space *** security analysis shows that the DISTINIT is secure against data poisoning attacks by considering key features of adversarial *** conclude that the proposed OJD-based DISTINIT is effective and efficient against data poisoning attacks where in-time detection is critical for IoT applications with large volumes of streaming data.

关键词： Data poisoning attacks detection framework jaccard distance(JD) optimized jaccard distance(OJD) security analysis

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巴基斯坦海德拉巴地区连续性斜视17例手术治疗临床观察(英文)

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国际眼科杂志 2009年第12期9卷 2265-2268页

作者： sameen Afzal Junejo Munawar Ahmed Arshad Ali Lodhi Liaquat大学医学院眼科医院

目的:观察接受斜视手术的连续性斜视患者的临床过程和治疗效果。方法:连续性斜视患者(<45岁)分为两组:其中第1组为内斜视患者;第2组为外斜视患者。我们使用棱镜屈光度(PD)来测量患者的偏斜角,对第一次手术后的患者在随访期间(6mo内)进... 详细信息

目的:观察接受斜视手术的连续性斜视患者的临床过程和治疗效果。方法:连续性斜视患者(<45岁)分为两组:其中第1组为内斜视患者;第2组为外斜视患者。我们使用棱镜屈光度(PD)来测量患者的偏斜角,对第一次手术后的患者在随访期间(6mo内)进行保守疗法。同时选择随访6mo后偏斜角仍超过15PD的斜视患者进行再次手术。本实验所有参与对象均进行了强制性检查。所有二次手术患者均在全身麻醉下进行,并于术后3,15d;3,6mo进行追踪随访。结果:在整个研究期间有28.8%的患者发展成为连续性斜视。所有斜视患者主视眼(固视眼)的二次手术均在一次手术后6～9mo内进行。经过二次手术干预后,在随访期间两组患者均获得了很好的治疗效果,同时并未出现过矫的趋势。结论:在二次手术过程中我们需谨慎肌肉矫正以避免日后过矫。

关键词：连续性斜视主视眼手术矫正海德拉巴

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Umbilical Cord Care Practices and Cord Care Education of Mothers Attending Health Care (Pakistan Prospect)

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Health 2023年第1期15卷 20-32页

作者： Muhammad Ans Mujahid Hussain Fahad Ahmed Kalsoom Jehan Khan sameen Abbas Muhammad Sultan University College of Pharmacy University of the Punjab Lahore Punjab Pakistan M.Phil. Health Economics Pakistan Institute of Development Economics (PIDE Isb) Islamabad Pakistan Faculty of Pharmacy University of Sargodha Sargodha Punjab Pakistan Lords College of Pharmacy University of the Punjab Lahore Punjab Pakistan Department of Pharmacy Quaid-I-Azam University Islamabad Pakistan

Neonatal mortality is of global concern, it is the death in the first month of life. According to WHO, 2.4 million children died globally in the first month of life2. Globally the neonatal mortality rate declined slowly from 28/100 live births in 2000 to 17.9/100 live births in 2019. It is a major challenge for the low socioeconomic group of countries. Most neonatal deaths occurred in south Asia and African countries, in 2019 out of 2.4 million death 81% occurred in the Southeast Asia. Objective: To evaluate and improve cord care practices of mothers to reduce the risk of cord related infections. Methodology: The mixed method will be used in this research, questionnaire is used for data collection, total collected sample is 391. Results: 354 (91.0%) of the 394 patients analyzed obtained prenatal care (ANC) in both traditional and nontraditional settings. Among these, 288 (72.3%) got cord care health education, whereas 106 (27.7%) did not. The overall documentation of the instructions for the use of metabolized spirits alone included 116 (48.9%) teaching institutions and 103 (43.5%) private hospitals. the average results show the age of the baby 1 - 7 days, the average age of the mother is 18 - 25 years. The average education of the mothers is primary. Participation of health centers is average. Most of the participants has 1 child. Conclusion: This research concludes the poor umbilical cord practices and knowledge. Those with information used it better. Raise awareness about umbilical cord care and improper behaviors, including using mentholated balms. Before women leave the hospital, they should learn cord care. Mothers must clean wires. Several healthcare organizations don’t provide cord care instruction, and what they provide isn’t research-based.

关键词： Knowledge Mothers Newborns Practices Umbilical Cord Care

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巴基斯坦海德拉巴地区儿童人群眼球开放伤(英文)

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国际眼科杂志 2009年第7期9卷 1226-1229页

作者： sameen Afzal Junejo Arshad Ali Lodhi Mahtab Alam Khanzada Liaquat大学医学院眼科医院

目的:探讨儿童眼球开放伤的发生原因;频率;严重程度;治疗;视力恢复及预防。方法:本研究包括15岁以下儿童眼球开放伤。使用裂隙灯检查角膜及巩膜裂伤,前房积血,虹膜脱出等。部分病例使用裂隙灯联合90D眼底镜检查眼底。经必要的检查后行... 详细信息

目的:探讨儿童眼球开放伤的发生原因;频率;严重程度;治疗;视力恢复及预防。方法:本研究包括15岁以下儿童眼球开放伤。使用裂隙灯检查角膜及巩膜裂伤,前房积血,虹膜脱出等。部分病例使用裂隙灯联合90D眼底镜检查眼底。经必要的检查后行急诊手术。结果:注册的43例(55%)儿童中,男占67%,女占33%。致伤物为玻璃,铅笔,木棍等。致伤部位为角膜占62.7%,巩膜占25.6%,角巩膜缘占11.7%。初次就诊伤眼视力6/60以上占32.5%,6/24以上占9.3%,6/12以上占2.3%。37.2%病例为光感。36例手术病例中有29例有术后随访。10.4%病例的最终视力为3/60,24.2%病例的最终视力为6/60,17.2%病例的最终视力为6/24。34.4%的病例的最终视力为光感。10.4%的病例发生眼球萎缩。结论:眼外伤是儿童人群中视力丧失的一个主要原因。在家庭及户外活动中采用必要的预防措施至关重要。

关键词：眼外伤儿童视功能失明

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Multisystem inflammatory syndrome in adults associated with COVID-19:A rare life-threatening complication

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Rheumatology & Autoimmunity 2023年第4期3卷 247-249页

作者： sameen Zafar Daniel Gonzalez Leonard Kuan-Pei Wang Alexandria Soybel Emilio B.Gonzalez Vijaya Murthy Department of Internal Medicine University of Texas Medical BranchGalvestonTexasUSA

To the Editor,Herein,we report a case of a 49-year-old Caucasian man with a medical history of gastroesophageal reflux disease,occasional premature ventricular contractions,and unvaccinated against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)who presented with myalgias,fevers,and right-sided neck swelling approximately 26 days after he had tested positive for SARS-CoV-2 and 21 days after receiving the casirivimab–imdevimab(REGEN-COV)*** initial workup showed leukocytosis and thrombocytopenia(platelets:108×109/L,reference range[RR]:150−328×109/L).The SARS-CoV-2 rapid antigen-detection test was *** patient was started on intravenous antibiotics for presumed lymphadenitis;however,his fevers persisted,with subsequent development of worsening thrombocytopenia at 91×109/L and increasing C-reactive protein(CRP)and erythrocyte sedimentation rate(ESR),with peak values at 13.4mg/dL(RR:<0.8 mg/dL)and 35 mm/1 h(RR:0−10 mm/1 h),respectively.

关键词： infusion sided swelling

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先天性上睑缺损患者21例手术结果分析(英文)

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国际眼科杂志 2009年第11期9卷 2063-2066页

作者： Arshad Ali Lodhi sameen Afzal Junejo Mahtab Alam Khanzada Imran Akram Sahaf Zahid Kamal Siddique Liaquat大学眼科医院眼科

目的:评估先天性上睑缺损患者手术修补的结果。方法:术前对所有患者进行完整的眼科及全身检查,麻醉下进行检查评估眼睑缺损的位置及大小,结膜受累情况。同时检查角膜状况,被动牵引实验检查眼球运动情况,根据缺损大小制定手术方案。结果... 详细信息

目的:评估先天性上睑缺损患者手术修补的结果。方法:术前对所有患者进行完整的眼科及全身检查,麻醉下进行检查评估眼睑缺损的位置及大小,结膜受累情况。同时检查角膜状况,被动牵引实验检查眼球运动情况,根据缺损大小制定手术方案。结果:21例先天性上睑缺损患者中,18例为独立发病的上睑中部缺损,13例双眼发病而5例为单眼。其余患者并发Goldenhar综合征及CHARGE综合征,患双眼上睑中部缺损。除了1例患者手术时为25岁,余所有患者进行手术矫正的年龄在2.5 ～4.0之间。1例患者已经存在角膜混浊,其他患者美容手术的效果满意。结论:本报道中,除了1例就诊晚而存在角膜缺陷外,所有手术效果满意。

关键词：缺损先天性上睑手术结果

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