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Detection of Glucomacropeptide in Raw Milk Adulterated with Cheese Whey in Ecuador

Food and Nutrition Sciences 2017年第6期8卷 579-590页

作者： Byron Humberto Puga-Torres samantha michele morales-arciniega Luis Fabián Núnez Naranjo David Isaías De la Torre-Duque Rolando Martin Campos-Vallejo Silvana Hipatia Santander-Parra Enrique César Vayas-Machado School of Veterinary Medicine and Animal Science Central University of EcuadorQuitoEcuador School of Engineering of Livestock Industries Chimborazo Polytechnic SchoolRiobambaEcuador Department of Experimental and Comparative Pathology School of Veterinary Medicine and Animal ScienceUniversity of Sao PauloSao PauloBrazil Career of Integral Agricultural Development Faculty of Agricultural Industries and Environmental SciencesCarchi Polytechnic UniversityTulcanEcuador

Milk is one of the products that can be adulterated in many ways affecting the quality of this and its derivatives. Glucomacropeptide (GMP) is a protein that is found only in the whey from the production of fresh cheese, enzymatically obtained from the coagulation of casein and which is commonly used to adulterate fresh or powdered milk. The aim of this study was to determine the adulteration of milk with cheese whey thought a molecular approach, where the glucomacropeptide was collected by sequential precipitation with trichloroacetic acid (ATC) and detected by polyacrylamidododecylsulfate gel electrophoresis (PAGE-SDS), using samples of fresh milk, intentionally adulterated with serum in the proportion of 0%, 1%, 5%, 10% and 15%. The results obtained showed that the detection of glucomacropeptide by electrophoresis was positive in all samples of adulterated milk, evidencing a band of 20.9 kDa in the reading, corresponding to the molecular weight of the GMP, showing that the technique used determines the adulteration in the milk, in a specific and sensitive way, also shows that in the evaluation of physical-chemical and microbiological parameters of milk, there are no significant differences between treatments, except for the pH that tends to decrease as the percentage of serum in the milk increases.

关键词： Glucomacropeptide Raw Milk Whey Cheese Milk Adulteration

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Gsα^(R201C)and estrogen reveal different subsets of bone marrow adiponectin expressing osteogenic cells

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Bone Research 2022年第4期10卷 784-798页

作者： Biagio Palmisano Rossella Labella samantha Donsante Cristina Remoli Emanuela Spica Ilenia Coletta Giorgia Farinacci michele Dello Spedale Venti Isabella Saggio Marta Serafini Pamela Gehron Robey Alessandro Corsi Mara Riminucci Department of Molecular Medicine Sapienza University of RomeRome 00161Italy Tettamanti Research Center Department of PediatricsUniversity of Milano Bicocca/Fondazione MBBMMonza 20900Italy Department of Biology and Biotechnology Sapienza University of RomeRome 00185Italy Institute of Structural Biology and School of Biological Sciences Nanyang Technological University 639798 SingaporeSingapore CNR Institute of Molecular Biology and Pathology Piazzale Aldo Moro 5Rome 00185Italy Skeletal Biology Section National Institute of Dental and Craniofacial ResearchNational Institutes of HealthDepartment of Health and Human ServicesBethesdaMD 20892USA

The Gsα/cAMP signaling pathway mediates the effect of a variety of hormones and factors that regulate the homeostasis of the post-natal ***,the dysregulated activity of Gsαdue to gain-of-function mutations (R201C/R201H)results in severe architectural and functional derangements of the entire bone/bone marrow *** the consequences of gain-of-function mutations of Gsαhave been extensively investigated in osteoblasts and in bone marrow osteoprogenitor cells at various differentiation stages,their effect in adipogenically-committed bone marrow stromal cells has remained *** generated a mouse model with expression of Gsα^(R201C) driven by the Adiponectin(Adq)***-Gsα^(R201C) mice developed a complex combination of metaphyseal,diaphyseal and cortical bone *** the metaphysis,Gsα^(R201C) caused an early phase of bone resorption followed by bone *** bone formation was sustained by cells that were traced by Adq-Cre and eventually resulted in a high trabecular bone mass *** the diaphysis,Gsα^(R201C),in combination with estrogen,triggered the osteogenic activity of Adq-Cre-targeted perivascular bone marrow stromal cells leading to intramedullary bone ***,consistent with the previously unnoticed presence of Adq-Cre-marked pericytes in intraosseous blood vessels,Gsα^(R201C) caused the development of a lytic phenotype that affected both cortical(increased porosity)and trabecular(tunneling resorption)*** results provide the first evidence that the Adq-cell network in the skeleton not only regulates bone resorption but also contributes to bone formation,and that the Gsα/cAMP pathway is a major modulator of both functions.

关键词： estrogen sustained skeleton

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Saharan dust clouds and paediatric asthma in the Caribbean Island of Trinidad

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Chinese Journal Of Geochemistry 2006年第B8期25卷 216-216页

作者： Keshav Gyan Winoah Henry Sherard Lacaille Anita Laloo Chela Lamsee-Ebanks samantha McKay Beverley Gulston Robin M. Antoine michele A Monteil School of Basic Sciences Faculty of Medical Sciences University of the West lndies St. Augustine Trinidad W.I. Department of Para-Clinical Sciences Faculty of Medical Sciences University of the West Indies Trinidad W.I Department of Mathematics ＆ Computer Sciences Faculty of Agriculture ＆ Natural Sciences University of the West Indies Trinidad W.I

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