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Leucine-rich repeat-containing G protein-coupled receptor 5 marks different cancer stem cell compartments in human Caco-2 and LoVo colon cancer lines

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World Journal of Gastroenterology 2021年第15期27卷 1578-1594页

作者： samah abdulaali alharbi Dmitry A Ovchinnikov Ernst Wolvetang Physiology Department College of MedicineUmm Al-Qura UniversityMakkah 24231Saudi Arabia Department of Stem Cell Engineering Group Australian Institute for Bioengineering and NanotechnologyThe University of QueenslandBrisbane 4072QLDAustralia Australian Institute for Bioengineering and Nanotechnology The University of QueenslandBrisbane 4072QLDAustralia

BACKGROUND Colon cancer cell lines are widely used for research and for the screening of drugs that specifically target the stem cell compartment of colon *** was reported that colon cancer carcinoma specimens contain a subset of leucine-rich repeatcontaining G protein-coupled receptor 5(LGR5)-expressing stem cells,these socalled“tumour-initiating”cells,reminiscent in their properties of the normal intestinal stem cells(ISCs),may explain the apparent heterogeneity of colon cancer cell ***,colon cancer is initiated by aberrant Wnt signaling in ISCs known to express high levels of ***,in vivo reports demonstrate the clonal expansion of intestinal adenomas from a single LGR5-expressing *** To investigate whether colon cancer cell lines contain cancer stem cells and to characterize these putative cancer stem *** A portable fluorescent reporter construct based on a conserved fragment of the LGR5 promoter was used to isolate the cell compartments expressing different levels of LGR5 in two widely used colon cancer cell lines(Caco-2 and LoVo).These cells were then characterized according to their proliferation capacity,gene expression signatures of ISC markers,and their tumorigenic properties in vivo and in *** The data revealed that the LGR5 reporter can be used to identify and isolate a classical intestinal crypt stem cell-like population from the Caco-2,but not from the LoVo,cell lines,in which the cancer stem cell population is more akin to B lymphoma Moloney murine leukemia virus insertion region 1 homolog(+4 crypt)stem *** sub-population within Caco-2 cells exhibits an intestinal cancer stem cell gene expression signature and can both self-renew and generate differentiated LGR5 negative *** data also show that cells expressing high levels of LGR5/enhanced yellow fluorescent protein(EYFP)from this cell line exhibit tumorigenic-like properties in vivo and in *** contrast,cell compartments of LoVo th

关键词： Colorectal cancer Colon cancer cell lines Intestinal stem cell Cancer stem cell Leucine-rich repeat-containing G protein-coupled receptor 5 Heterogenicity

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