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Knockdown of GmSOG1Compromises Drought Tolerance in Transgenic Soybean Lines

American Journal of Plant Sciences 2021年第1期12卷 18-36页

作者： sam.el aduse poku Peter Nkachukwu Chukwurah Htut Htet Aung Ikuo Nakamura Laboratory of Plant Cell Technology Chiba University Chiba Japan

Plants are regularly exposed to myriads of stress factors that cause tremendous damage to their genetic make-up. To ensure genome stability and survival over several generations under harsher environmental conditions, plants have evolved a unique mechanism for dealing with DNA damage known as the DNA damage response pathway (DDR). It has been proposed that there may exist a relationship between the DNA damage response pathway and abiotic stress response in plants. To further investigate this relationship, we knocked down the soybean suppressor of gamma response 1 gene (GmSOG1), a master regulatory gene of the DDR, in soybean plants and subjected the generated transgenic plants to drought stress analysis. Gene expression analysis of the GmSOG1 gene in drought stressed soybean tissues revealed high levels of expression in buds and young leaves. The root lengths and root fresh weights of transgenic soybean plants grown on Murashige and Skoog media supplemented with Gamborg B5 vitamins (MSB5 media) containing 200 mM mannitol for 10 days were significantly lesser than those of drought stressed wild-type plants. Polyethylene glycol (PEG) induced drought stress assay in vivo resulted in significant damage in transgenic plants compared with wild-type plants. Also, the relative expressions of known drought responsive transcription factors such as GmDREB1 and GmLEA as well as antioxidation related genes like

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Mitigating Root Knot Nematode Propagation on Transgenic Tobacco via in PlantaHairpin RNA Expression of Meloidogyne incognita—Specific PolA1 Sequence

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American Journal of Plant Sciences 2019年第5期10卷 866-884页

作者： Peter Nkachukwu Chukwurah sam.el aduse poku Akira Yokoyama Ai Takeda Masahiro Shishido Ikuo Nakamura Laboratory of Plant Cell Technology Chiba University Matsudo Japan Department of Genetics and Biotechnology University of Calabar Calabar Nigeria Laboratory of Plant Pathology Chiba University Matsudo Japan Chiba Prefectural Agriculture and Forestry Research Center Daizennocho Chiba Japan

Root knot nematodes are top priority nematode pests that significantly constrain agricultural productivity globally especially in developing countries. However, expressing double stranded RNA (dsRNA) of essential nematode genes in susceptible plants is known to confer protection against these pests via RNA silencing. This molecular-based strategy is called host induced gene silencing (HIGS) and the selection of appropriate target nematode gene is critical to its success. In this study, therefore, we focused on root knot nematode PolA1, an essential single copy nuclear gene encoding the largest subunit of RNA polymerase I enzyme and evaluated its effectiveness as a target in conferring nematode resistance on Agrobacterium-mediated transformed tobacco plants. Transgenic tobacco expressing Meloidogyne incognita-specific (MiS) dsRNA of PolA1 gene showed significant reduction in nematode fecundity and multiplication compared to wild type plants in both T0 and T1 generations. T0 plants showed varying degrees of agronomic vigorover WT plants possibly due to varying levels of processed siRNA. However, production of MiS siRNAs in the transgenic plants coupled with significant reduction of PolA1 transcript expression in nematodes feeding on roots of transgenic plants provided evidence of HIGS. Taken together, our results show that PolA1 is a potentially effective target for HIGS-mediated reduction of root knot nematode damage on transgenic tobacco. Given the homology of our target sequence among Meloidogyne species, this protection could be broad range against other root knot nematodes aside M. incognita.

关键词： M. incognita Nematode Resistance N. tabacum RNA Silencing PolA1 Gene

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Global wild rice germplasm resources conservation alliance:World Wild-Rice Wiring

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Molecular Plant 2024年第4期17卷 516-518页

作者： Xiaoming Zheng Disna Ratnasekera Jiayu Fan Robert J.Henry Beng-Kah Song Kenneth M.Olsen Bal Krishna Joshi Maria Celeste N.Banaticla-Hilario Tonapha Pusadee Adane Getachew Melaku Yeyinou Laura Estelle Loko Koukham Vilayheuang Gavers K.Oppong sam.el aduse poku Peterson W.Wambugu Song Ge Aldo Merotto Junior Ohn Mar Aung Ramaiah Venuprasad Ajay Kohli Wenbin Zhou Qian Qian National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Sanya National Research Institute of Breeding in Hainan Chinese Academy of Agricultural SciencesBeijing 100081China International Rice Research Institute DAPO Box 7777Metro Manila 4031Philippines Department of Agricultural Biology Faculty of AgricultureUniversity of RuhunaMatara 81000Sri Lanka Yazhouwan National Laboratory No.8 Huanjin RoadYazhou DistrictSanya CityHainan Province 572024China Hainan University Haikou 570228China ARC Centre of Excellence for Plant Success in Nature and Agriculture University of QueenslandBrisbaneQLD4072Australia School of Science Monash University Malaysia47500 Bandar SunwaySelangor 47500Malaysia Department of Biology Washington University in St.LouisSt.LouisMO 63130-4899USA National Agriculture Genetic Resources Center(Genebank) KhumaltarPO Box 3055KathmanduNepal Plant Biology Division Insttute of Biological SciencesUniversity of the Pilippines Los BanosLos BanosLaguna 4031Philippines Division of Agronomy Department of Plant and Soil SciencesFaculty of AgricultureChiang Mai UniversityChiang Mai 50200Thailand Bio and Emerging Technology Institute Addis Ababa 1165Ethiopia National University of Sciences TechnologiesEngineering and Mathematics(UNSTIM)Dassa-ZouméBP 14Benin National Genebank Rice and Cash Crops Research CenterNAFRIVientiane 0605Lao PDR Plant Sciences and the Bioeconomy Rothamsted ResearchHarpenden AL52JQUK UK Future Food Beacon of Excellence and School of Biosciences and University of Nottingham Nottingham LE125RDUK Department of Plant and Environmental Biology University of GhanaAccraGhana Kenya Agricultural and Livestock Research Organization Genetic Resources Research InstituteNairobi 00200Kenya Institute of Botany Chinese Academy of SciencesBeijing 100093China Federal University of Rio Grande do Sul UFRGSPorto Alegre 91501-970RSBrazil Department of Agricultur

Wild relatives of crop are key genetic resources serving as diversity reservoirs for crop improvement under changing ***(Oryza sativa)is one of the most important crops in the world,providing staple food for half of the world's *** rice is thus a critical germplasm resource for sustained global food security,ensuring high production yields,improved quality,and stress resistance in the face of climate *** rice is closely related to domesticated rice and has a rich genetic diversity and exceptional adaptability to extreme *** has played a pivotal role in the history of rice hybridization and has become a key resource for rice breeding *** identification of wild-type cytoplasmic male sterility resources paved the way for the achievement of the“three lines”goal in hybrid rice,leading to a significant increase in rice *** addition,the use of resistance alleles found in wild rice is making rice production more resilient to losses caused by environmental ***,wild rice germplasm resources are threatened due to habitat destruction and other anthropogenic *** the same time,the lack of centralized distribution of wild rice has hampered the sharing of basic information on wild rice resources and the utilization and conservation of wild rice in each country,as well as collaboration among scientists.

关键词： germplasm breeding crops

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