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Immune response to hepatitis B virus vaccine in celiac subjects at diagnosis

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World Journal of Hepatology 2016年第26期8卷 1105-1109页

作者： Martina Filippelli Maria Teresa Garozzo Antonino Capizzi Massimo Spina Sara Manti Lucia Tardino Carmelo Salpietro salvatore leonardi Depart-ment of Medical and Pediatric Sciences University of Catania Department of Pediatric Sciences University of Messina

AIM To evaluate hepatitis B virus(HBV) vaccine response and correlation with human leukocyte antigens(HLA) and/or gluten intake in celiac patients at *** Fifty-one patients affected by celiac disease, diagnosed at the Department of Pediatrics of the University of Catania(Italy), were recruited. All patients were tested at admission for immunization against HBV, according to findings from analysis of quantitative HBV surface antibody(anti-HBs). The anti-HBs titer was measured by enzyme-linked immunosorbent assay. Following the international standards, subjects with antibody titer 0.05). With regard to the HLA haplotype, comparison of the distribution of vaccination response showed no statistically significant difference between the different genotypes(homozygosity for the HLADQ2 haplotype compared with HLADQ2/DQ8 heterozygosity or other haplotypes; P > 0.05). Moreover, distribution of the responders according to clinical features of celiac disease showed no statistically significant differences(P > 0.05).CONCLUSION This prospective study confirmed the lower percentage of response to HBV vaccine in celiac subjects. However, the underlying mechanism remains unclear and further studies are needed.

关键词： Celiac disease Hepatitis B virus vaccination Human leukocyte antigens Gluten Poor response

Sexual outcomes after partial penectomy for penile cancer： results from a multi-institutional study

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Asian Journal of Andrology 2017年第1期19卷 57-61页

作者： salvatore Sansalone Mauro Silvani Rosario leonardi Giuseppe Vespasiani Valerio Iacovelli Department of Experimental Medicine and Surgery - Urology Tor Vergata University of Rome 00133 Rome Italy Department of Urology Biella General Hospital 13900 Biella Italy Musumeci GECAS Clinic Gravina di catania 95127 Catania Italy.

Penile cancer is an uncommon malignancy. Surgical treatment is inevitably mutilating. Considering the strong impact on patients＇ sexual life we want to evaluate sexual function and satisfaction after partial penectomy. The patients in this study （n = 25） represented all those who attended our institutions and were diagnosed and treated for penile cancer from October 2011 to November 2013. All patients underwent partial penectomy and followed-up （mean： 14 months; range： 12-25）. Sexual presurgical baseline was estimated using the International Index of Erectile Dysfunction 15 （IIEF-15）. Sexual outcomes of each patient were estimated considering four standardized and validated questionnaires. We analyzed the means and ranges of IIEF-15 including erectile function （IIEF-1-5 and -15）, orgasmic function （IIEF-9 and -10）, sexual desire （IIEF-11 and -12）, intercourse satisfaction （IIEF-6-8）, and overall satisfaction （IIEF-13 and -14）. Then, we also used Quality of Erection Questionnaire （QEQ）, Erectile Dysfunction Inventory of Treatment Satisfaction （EDITS） and Self-Esteem and Relationship （SEAR） to evaluate the sexual function and satisfaction of our patients. The final results showed that penile cancer leads to several sexual and psychosexual dysfunctions. Nevertheless, patients who undergo partial penectomy for penile cancer can maintain the sexual outcomes at levels slightly lower to those that existed in the period before surgery.

关键词： partial penectomy penectomy penile cancer penile tumor sexual outcomes

Microsurgical tunica albuginea transplantation in an animal model

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Asian Journal of Andrology 2017年第6期19卷 694-699页

作者： salvatore Sansalone Carla Loreto Rosario leonardi Giuseppe Vespasiani Giuseppe Musumeci Claudia Lombardo Sergio Castorina Venera Cardile Rosario Caltabiano Department of Experimental Medicine and Surgery Tor Vergata University Rome Italy Department of Biomedical and Technological Sciences Section of Human Anatomy and Histology University of Catania Catania Italy Musumeci CLinic GECS Catania Italy Department of Bio-Medical Sciences Section of Physiology University of Catania Catania Italy Department of Medical and Surgical Sciences and Advanced Technology "G.E Ingrassia" Section of Anatomic Pathology University of Catania Catania Italy.

Several andrological diseases require surgical repair or reconstruction of tunica albuginea, which envelops the corpora cavernosa penis. Despite intense research efforts involving a variety of biological materials, such as skin, muscle aponeurosis, human dura mater, tunica vaginalis, and pericardium, engineered tunica albuginea suitable for graft use is yet to be obtained. The study investigates microsurgical tunica albuginea allotransplantation in an animal model with the purpose of creation of an organ-specific tissue bank to store penile tissue, from cadaveric donors and male-to-female trans-sexual surgery, for allogeneic transplantation. Materials were tunica albuginea tissue explanted from 15 donor rats, cryopreserved at -80~C, gamma-irradiated, and implanted in 15 recipient rats, of which three rats were used as controls. Penile grafts were explanted at different time intervals; after macroscopic evaluation of the organ, the grafts were processed to morphological, histochemical, and immunohistochemical examinations by light microscopy. Detection of pro-inflammatory cytokines was also performed. Examination of the tunica albuginea allografts collected 1, 3, or 6 months after surgery and of control tunica albuginea fragments showed that tunica albuginea implants achieved biointegration with adjacent tissue at all-time points. The integration of cryopreserved rat tunica albuginea allografts, documented by our study, encourages the exploration of tunica albuginea allotransplantation in humans. In conclusion, the effectiveness and reliability of the tunica albuginea conditioning protocol described here suggest the feasibility of setting up a tunica albuginea bank as a further tissue bank.

关键词： penile tunica albuginea rat model transplant

TRAIL Immunolocalisation in the Rat Periodontal Ligament during Experimental Tooth Movement. A Preliminary Study

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Open Journal of Apoptosis 2013年第3期2卷 31-36页

作者： Carla Loreto Giuseppe Musumeci Sergio Castorina Jessica Valentino salvatore Giunta Rosalia leonardi Department of Bio-Medical Sciences Anatomy and Histology Section University of Catania Catania Italy Department of Orthodontics University of Catania Catania Italy

The aim of this study was to evaluate immunohistochemically TRAIL (TNF-Related Apoptosis Inducing Ligand) expression in the periodontal ligament of rats’ molars considering its distribution pattern in the tension and compressive regions of ligament during orthodontic movement. Sixteen Sprague-Dawley rats, weighing between 120 and 200 gwere used in the present study. Tooth movement was induced by placing elastic bands between the maxillary first and second molars. The elastic bands were left in place for 12 (time T1) and 24 (time T2) hours. After rats’ euthanasia, maxillae were dissected free and the sections were exposed to a primary rabbit polyclonal anti-TRAIL anti-body. The staining status was identified as either negative or positive by using 3,3’-diaminobenzidine (DAB) to develop the immunoreaction. Data were analyzed using ANOVA test and a post-hoc Dunn’s Multiple Comparison test. P-values of less than 0.05 were considered statistically significant. The staining for TRAIL in the tension side appeared darker (intensity of staining = 2) than in the compressive side (intensity of staining = 1) 12 h after the tooth movement. The stainings of both sides of PDL were observed to be up-regulated 24 h after the application of the force (tension side = 3, compression side = 2;P 0.05). TRAIL is expressed in the periodontal ligament of rats’ molars during experimental tooth movement both in the compression and in the tension sides. The latter showed the highest amount of TRAIL immunolabeling.

关键词： Immunohistochemistry Pperiodontal Ligament Tooth Movement Trail