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A novel HPTLC method for quantitative estimation of biomarkers in polyherbal formulation

Asian Pacific Journal of Tropical Biomedicine 2015年第11期5卷 955-959页

作者： Zeeshan Ahmed Sheikh Sadia Shakeel Somia Gul Aqib Zahoor saleha suleman khan Faisal Haider Zaidi khan Usmanghani Herbion Pakistan (Pvt.) Ltd. Dow College of Pharmacy Dow University of Health Sciences Faculty of Pharmacy Jinnah University for Women Karachi Department of Basic Medical Sciences College of MedicineKing Saud bin Abdulaziz University of Health Sciences

Objective: To explore the quantitative estimation of biomarkers gallic acid and berberine in polyherbal formulation Entoban ***: High performance thin layer chromatography was performed to evaluate the presence of gallic acid and berberine employing toluene: ethyl acetate: formic acid:methanol 12:9:4:0.5(v/v/v/v) and ethanol: water: formic acid 90:9:1(v/v/v), as a mobile phase ***: The R f values(0.58) for gallic acid and(0.76) for berberine in both sample and reference standard were found comparable under UV light at 273 nm and 366 nm respectively. The high performance thin layer chromatography method developed for quantization was simple, accurate and ***: The present standardization provides specific and accurate tool to develop qualifications for identity, transparency and reproducibility of biomarkers in Entoban syrup.

关键词： Quantitative estimation Gallic acid Berberine Ento

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Design, Development and Phytochemical Evaluation of a Poly Herbal Formulation Linkus Syrup

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Chinese Medicine 2014年第2期5卷 104-112页

作者： Zeeshan Ahmed Sheikh Aqib Zahoor saleha suleman khan khan Usmanghani Department of Research and Development Herbion Pakistan (Pvt.) Limited 30/28 Korangi Industrial Area Karachi Pakistan Department of Research and Development Herbion Pakistan (Pvt.) Limited 30/28 Korangi Industrial Area Karachi Pakistan Faculty of Pharmacy Jinnah University for Women Karachi Pakistan

The present work has been carried out on polyherbal formulation named as Linkus Syrup. The herbal formulation consists of Glycyrrhiza glabra, Hyssopus officinalis, Piper longum and Alpinia galangal. The Linkus syrup physico-chemical evaluation such as pH, density, identification of polysaccharide, tanning agents, ascorbic acid and shelf life was complied. The TLC and quantitative determination of alkaloid were quantified. Determination of biomarker has been validated for the analysis of vasicine. The study result revealed that Linkus syrup formulation was well standardized at different levels such as physic-chemical consistency and assay of bio marker compound.

关键词： Syrup Crude Herb Anti-Tussive Anti-Bronchitis Anti-Pharyngitis Sore Throat Tracheitis HPLC

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Quantitative Estimation of Gallic Acid as Biomarker in Lipitame Tablets by HPTLC Densitometry for Diabetic Dyslipidemia

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Chinese Medicine 2014年第4期5卷 170-178页

作者： Sheeraz Siddiqui khan Usmanghani Aqib Zahoor Zeeshan Ahmed Sheikh saleha suleman khan Faculty of Eastern Medicine Hamdard University Karachi Pakistan Research and Development Department Herbion Pakistan (Pvt.) Limited Karachi Pakistan

Lipitame is a poly herbal formulation comprised of Terminalia arjuna, Terminalia belerica, Commiphora mukul and Phyllanthus emblica. The formulation is investigated for its analysis evaluation. Biomarkers of three herbs such as Terminalia arjuna, Terminalia belerica, and Phyllanthus emblica have been already cited to contain gallic acid, which was qualitatively and quantitatively estimated. In the present study rapid and inexpensive qualification methods for the quality control of Terminalia arjuna, Terminalia belerica, Commiphora mukul and Phyllanthus emblica on thin layer chromatography (TLC) were developed and validated. The solvent system used was toluene:ethyl acetate:formic acid:methanol (12:9:4:0.5). The scanning of plate was performed linearly at 273 nm (absorption) by use of a TLC Scanner III CAMAG with a deuterium source, and the area of spots corresponding to Gallic acid standard was integrated. It was found that gallic acid has been found in the Lipitame tablets on HPTLC densitometry assessment compared with authentic gallic acid reference standard.

关键词： Lipitame Tablets Quantitative Estimation Densitometry Diabetic Dyslipidemia

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