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Genetic Diversity.of the Palestinian Fig (<i>Ficus carica</i>L.) Collection by.Pomological Traits and RAPD Markers

American Journal of Plant Sciences 2014年第9期5卷 1139-1155页

作者： Mohammed S. Ali-Shtay.h Rana M. Jamous salam y. ab. zaitoun Omar B. Mallah Ay.t Kh. Mubaslat Biodiversity.and Biotechnology.Research Unit Biodiversity and Environmental Research Center (BERC) Nablus Palestine

Analy.is of differentiation (genetic diversity.and related relationships) among 22 landrace (Ficus carica L. sativa) and 2 wild form (F. carica L. caprificus) accessions of fig growing under the same environmental conditions in the Palestinian Fig Collection, Til, Nab.us, Palestine, using PCR-based Random Amplified Poly.orphic DNA (RAPD) and pomological markers, revealed considerab.e genetic diversity. The phenoty.ic analy.is shows that pomological traits were permitted to evaluate morphological variab.lity.of fig landraces. The Jaccard similarity.coefficient between landraces was determined by.cluster analy.is using the UPGMA method. Based on the genetic relationships among genoty.es as illustrated by.the dendrograms, generated from pomological and RAPD data by.UPGMA clustering method, the following 12 genoty.es: Qaisi, Mwazi, Barqawi, Inaqi, Swadi, Kharobi, Hmadibiadi, Sfari, Khdari, Biadi, Qrawi, and Slati, may.be considered as distinct landraces. The remaining genoty.es may.be considered as sy.ony.ous (4) (Hmadi and Hmari, and Ajloni and Adloni), or closely.related (6) landraces (Zraqi and Ghzali, Blati and Neami, and Qraee and Khurtmani). The wild fig forms clustered together and may.be considered as distinct genoty.es. Clustering patterns obtained from the combined (pomological and RAPD) markers had higher discriminatory.power to discriminate fig landraces than using either pomological or RAPD markers alone. These results proved the importance of both pomological and RAPD markers to elucidate in part denomination problems and relationships among cultivars. Wide phenoty.ic and molecular diversity.found in fig germplasm indicates a considerab.e potential for improving this crop.

关键词： Fig DNA Markers Genetic Diversity RAPD Pomological Traits Palestine

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The effect of rosin acid on hy.rodeoxy.enation of fatty.acid

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Journal of Energy.Chemistry 2019年第1期28卷 85-94页

作者： Houman Ojagh Derek Creaser Muhammad ab.us salam Eva Lind Grennfelt Louise Olsson Competence Centre for Cataly.is Chemical Engineering Chalmers University of Technology Preem ab

In this study. inhibition of tall oil fatty.acid hy.rodeoxy.enation(HDO) activity.due to addition of rosin acid over sulfided Ni Mo/Al_2O_3 was investigated. Oleic acid and ab.etic acid were used as model compounds for fatty.acid and rosin acid respectively.in tall oil. After completion of each HDO experiment,the Ni Mo cataly.ts were recovered and used again under the same conditions. The results showed that the oleic acid HDO activity.of sulfided cataly.ts was inhibited by.addition of ab.etic acid due to competitive adsorption and increased coke deposition. The rate of carbon deposition on the cataly.ts increased when ab.etic acid was added to oleic acid feed. Moreover, the coke was in a more advanced form with higher stab.lity.for the cataly.ts exposed to both oleic acid and ab.etic acid. Furthermore, a clear correlation between the rate of coke formation and concentration of ab.etic acid was observed.

关键词： Rosin acid Carbon deposition Coke formation Hy.rodeoxy.enation(HDO) Inhibition

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Evaluation of Two Serological Screening Kits for Hepatitis C Virus Infection at the Regional Blood Transfusion Center of Ouagadougou, Burkina Faso

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Advances in Infectious Diseases 2020年第4期10卷 216-227页

作者： Arzouma Paul y.oda Koumpingnin Nebie Juliette Tranchot-Diallo salam Sawadogo Moutanou Modeste Judes Zey. ab.oul-Guaniy. Sawadogo ab.ou Azaque Zoure Dinanibè Kambire Serge Sawadogo Seimbou Zalla y.tema Dieudonné y.nli ab.bou Simpore Sibiri Nana Ashmed Chèickh Bachirou Nana Anita Pierrette Siritie Fiffou y.ugbare Sonia Ba-Nébhane Sontie Alain Konsey.o Jean Etienne Koanda Oury.Sanou Jacques Simpore National Blood Transfusion Center (NBTC) Ouagadougou Burkina Faso Lab.ratory.of Molecular Biology.and Genetics (Lab.OGENE) Joseph KI-ZERBO University Ouagadougou Burkina Faso Training and Research Unit/Science and Technology.(TRU/ST) Nazi-BONI University Bobo-Dioulasso Burkina Faso Research Institute in Health Sciences (IRSS/CNRST) Ouagadougou Burkina Faso

Introduction: In Burkina Faso, screening for hepatitis C virus in blood donations is made using sensitive ELISA (Enzy.e Linked Immuno Sorbent Assay. ty.e kits. However, no confirmation of the positive results obtained with these kits is made before their notification to the blood donors due to the high costs of the confirmation kits of immunoblots ty.e. Objective: Evaluate two rapid kits against one immunoblot kit in order to determine the most efficiency.which will be proposed as an alternative for the confirmation of ELISA tests in the socio-economic context of Burkina Faso. Material and Methods: The study.was carried out using a panel of 72 sera, of which 22 were positive for anti-HCV antibodies and 50 were negative. The sera were tested using the Monolisa® HCV Ag-ab.ULTRA kit and confirmed with the DECISCAN HCV Plus kit. The panel was then tested with the SD BIOLINE HCV kit and the HCV TRI-DOT kit and the results obtained were evaluated against those of the DECISCAN HCV Plus kit used as “gold standard”. Results: Compared to the DECISCAN HCV Plus kit, the HCV TRI-DOT kit exhibited a sensitivity.and specificity.of 100% and the SD BIOLINE HCV kit a sensitivity.of 86.36% and a specificity.of 100%. Conclusion: Based on the results recorded by.the HCV TRI-DOT kit, it would be best suited to the sero-epidemiological context of blood donors from the National Blood Transfusion Center and could be proposed as an alternative for confirmation of ELISA tests.

关键词： HCV Sensitivity Specificity Burkina Faso

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