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Morphogenetic Study of Pepino and Other Members of Solanaceae Family

American Journal of Plant Sciences 2014年第26期5卷 3761-3768页

作者： Habib ahmad Ayaz Khan Khushi Muhammad Muhammad Shahid Nadeem Waqar ahmad sajjad Iqbal Aniqa Nosheen Nazia Akbar Israr ahmad Youxiong Que Department of Genetics Hazara University Manshera Pakistan Institute of Chemical and Biological Sciences Department of Botany University of the Gujrat Gujrat Pakistan Key Lab of Sugarcane Biology and Genetic Breeding Ministry of Agriculture Fujian Agriculture and Forestry University Fuzhou China

This paper reports the findings regarding morphology, DNA markers and fruit quality of Solanum muricatum L., a newly introduced fruit in Hazara University, Pakistan, and other locally grown members of the Solanaceae family. In this study, RAPD (Random Amplified Polymorphic NA) based PCR report on phylogenetic affinities of pepino with brinjal, chilies, potato and tomato was also included. Total genomic DNA was isolated and 120 arbitrary decamers were applied to the genomic DNA of all these species. Only five out of the 120 decamers produced 27 polymorphic loci with the range of 400 - 1400 bp. We observed the specificity of markers, and primer B-08 amplified 700 bp locus linked to Solanum muricatum L., (Pepino), which could be used to identify this particular species of this family. Cluster analysis was also performed using the DNAMAN software (version 5.***.2.0) against the bivariate data collected from the products of several decamers. The UPGMA (Unweighted pair Group Method with Arithmetic Mean) analyses depicted three distinct groups, i.e. group I sorted out brinjal-tomato, group II sorted out chili-potato and pepino sorted out into its independent group III. Though on the basis of morphological traits pepino clustered with tomato, its DNA analyses proved it as a distinct species, far more related to the tomato. Moreover a total six parameters in biochemical analysis were studied, which revealed that pepino is a juicy fruit with maximum 93.25% moisture. This study could be helpful to maximize the new crop pepino in Pakistan.

关键词： Phylogeny Genetic Identity PCR Pepino RAPD

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含电弧炉钢渣粗骨料的温拌沥青混合料长期老化评价

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中外公路 2018年第4期38卷 262-267页

作者：钱国平(编译) 朱海(编译) sajjad Masoudi Sayyed Mahdi Abtahi ahmad Gdi 长沙理工大学交通运输工程学院湖南长沙410004 不详

为达到节能减排的目的,用次级材料代替矿物骨料降低沥青混合料的生产温度,该文通过马歇尔稳定度试验、25℃和40℃下的弹性模量试验、间接拉伸强度试验和水稳定性试验,研究添加电弧炉钢渣的温拌沥青混合料的长期性能。结果表明:采用钢渣... 详细信息

为达到节能减排的目的,用次级材料代替矿物骨料降低沥青混合料的生产温度,该文通过马歇尔稳定度试验、25℃和40℃下的弹性模量试验、间接拉伸强度试验和水稳定性试验,研究添加电弧炉钢渣的温拌沥青混合料的长期性能。结果表明:采用钢渣骨料温拌沥青增加了沥青混合料的马歇尔稳定度、刚度、弹性模量和间接拉伸强度,与对照样品相比,钢渣温拌沥青混合料老化程度降低,性能得到提升。

关键词：钢渣 Sasobit 温拌沥青老化弹性模量

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New Synthesis of Pt-Ru Nanoparticles on Surface Modified Carbon Vulcane XC-72 as an Effective Catalyst for Direct Methanol Fuel Cell

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复旦学报（自然科学版） 2007年第5期46卷 827-页

作者： ahmad Nozad Golikand sajjad Sadaghat Sharehjini Mohammad Yari Islamic Azad University Shahryar Shahr-e-Qod Branch Tehran

1 Results Pt-Ru nanoparticles are synthesised on the surface oxidized carbon Vulcane XC-72 as catalyst support by chemically anchoring Pt and Ru onto the surface of modified carbon vulcane XC-72 (by refluxing in 70% HNO3 at 120 ℃ for 12 h to introduce surface functional groups) .The nanoparticles of Pt and Ru are synthesized by reduction of H2PtCl6 and K4Ru(CN)6 with sodium borohydride in a 5.5 buffer solution of sodium citrate,the complexation of citrate with metal ions is beneficial to the formati...

关键词： carbon vulcane XC-72 Pt-Ru nanoparticles fuel cell methanol electro-oxidation

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Platinium Nanoparticles Deposited on Oxygen-Containing Functional Groups at Carbon Vulcane XC-72 as a Cathode Catalyst for Direct Methanol Fuel Cell

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复旦学报（自然科学版） 2007年第5期46卷 840-页

作者： sajjad Sadaghat Sharehjini ahmad Nozad Golikand Mohammad Yari Islamic Azad University Shahryar Shahr-e-Qods and Islamshahr Branch Tehran

1 Results Surface oxidized carbon vulcane XC-72 as catalyst support, prepared by chemically anchoring Pt onto the surface modified carbon vulcane XC-72. The nanoparticles of Pt are synthesized by reduction of H2PtCl6 with sodium borohydride in a 5.5 buffer solution of sodium citrate, the complexation of citrate with metal ions is beneficial to the formation of nanoparticles. For comparison, an electrode is prepared by E-Tek Pt/C 20 Wt% with a typical Pt loading of 50 μg·cm-2, that shows higher specifi...

关键词： modified carbon vulcane XC-72 Pt nanoparticles fuel cell methanol electro-oxidation

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Numerical Solution for a Similar Flow between Two Disks in the Presence of a Magnetic Field

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Applied Mathematics 2013年第8期4卷 1163-1167页

作者： sajjad Hussain Muhammad Anwar Kamal Farooq ahmad Muhammad Ali Muhammad Shafique Sifat Hussain Centre for Advanced Studies in Pure and Applied Mathematics Bahauddin Zakariya University Multan Pakistan Department of Mathematics Gomal University D. I. Khan Pakistan Department of Mathematics King Suleman Bin Abdulaziz University Al-Kharj Saudi Arabia Department of Mathematics Minhaj University Lahore Pakistan

Numerical solutions are obtained for non-steady, incompressible fluid flow between two parallel disks which at time t are separated by a distance H(1-αt)1/2 and a magnetic field proportional to B0(1-αt) -1/2 is applied perpendicular to the disks where H denotes a representative length, BO denotes a representative magnetic field and α-1 denotes a representative time. Similarity transformations are used to convert the governing partial differential equations of motion in to ordinary differential form. The resulting ordinary differential equations are solved numerically using SOR method, Richardson extrapolation and Simpson’s (1/3) Rule. Our numerical scheme is straightforward, efficient and easy to program.

关键词： Numerical Solution Similar Flow Newtonian Fluid Magnetic Field Reynold’s Number

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The Carbon Sinks and Mitigation Potential of Deodar (<i>Cedrus deodara</i>) Forest Ecosystem at Different Altitude in Kumrat Valley, Pakistan

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Open Journal of Forestry 2018年第4期8卷 553-566页

作者： Adnan ahmad Muhammad Amir Abdul Mannan sajjad Saeed Sher Shah Sami Ullah Rahman Uddin Qijing Liu Department of Forest Sciences Beijing Forestry University Beijing China Shaheed Benazir Bhutto University Sheringal Dir Upper Pakistan Beijing Key Laboratory of Precision Forestry Beijing Forestry University Beijing China

Forest carbon monitoring and reporting are critical for informing global climate change assessment. The regional estimates of forest carbon attached greater attention, to assess the role of forest in carbon mitigation. Here using field inventory, we examined the carbon sink and mitigation potential of monospecific Deodar forest in the Kumrat valley, of Hindu Kush Himalaya, Region of Pakistan, at a different elevation. The elevation of monospecific Deodar forest ranges from 2300 to 2700 m (a.s.l). We divided the forest into three elevation classes (that is 2300 - 2400 m (EI) 2400 - 2500 m (EII) and 2500 - 2700 m (EIII) a.s.l respectively). In each elevation class, we laid out 09 sample plots (33*33 m2) for measuring carbon values in living tree biomass (LT), soil (SC), litter, dead wood, cone (LDWC) and understory vegetation (USV). Our results showed that the carbon density at EI was 432.37 ± 277.96 Mg·C-1, while the carbon density at EII and EIII was 668.35 ± 323.94 and 1016.79 ± 542.99 Mg·C-1 respectively. Our finding revealed that the carbon mitigation potential of the forest increases with increasing elevation. Among the different elevation classes, EIII stored significantly higher carbon due to the dominance of mature, old age, larger trees, and the minimum anthropogenic disturbance, whereas EI stored statistically lower carbon because of maximum anthropogenic disturbance, which resulted in the removal of mature and over-mature trees. Furthermore, our correlation analysis between tree height and carbon stock and basal area and LT carbon, underlines that the basal area is the stronger predictor of LT carbon estimation than height. Overall our results highlight that deodar forest stored 716.94 ± 462.06 Mg?C·ha-1. However, the rehabilitation, preservation and sustainable management of disturb forest located at a lower elevation could considerably improve carbon mitigation potential.

关键词： Deodar Forest Elevation Carbon Sinks Mitigation Potential

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Molecular Detection and Sequencing for S1 Glycoprotein Gene of Bronchitis Virus of 2016 Epidemic from Sindh and Punjab

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Advances in Bioscience and Biotechnology 2018年第12期9卷 649-660页

作者： ahmad Umer Sultan Muhammad Danish Mehmood Rameez Hassan Huma Anwar Sana Noreen Faisal Amin sajjad Hussain Ottoman Pharma (Immuno Division) Lahore Pakistan Institute of Molecular Biology and Biotechnology University of Lahore Lahore Pakistan Grand Parent Diagnostic Laboratory Lahore Pakistan Veterinary Research Institute Lahore Pakistan

Infectious Bronchitis (IB) is highly contagious disease of commercial poultry causing substantial economic loses by producing poor quality meat in broilers and effecting production in breeder birds. The causative agent has been reported as most hazardous pathogen among other infectious agent even after being immunized with multi-variant strain vaccine. Currently, different strain such as H-120, 4/91 and D274 have been used extensively for immunoprophylaxis against velogenic strain across Pakistan with minimal protection reported. In current study PCR analysis was used to investigate the molecular nature of IB isolates from Punjab and Sind province of Pakistan in 2016 epidemics. Total of 100 tracheal samples were considered for virus inoculation in 10 days old chicken embryonated eggs. The IBV infected amniotic fluid was neutralized with monoclonal antisera of H-120, 4/91 and D274 strains. The IBV screened samples were subjected for RNA extraction and subsequent to PCR using type specific primer of each strain. The amplified product of 840 bp was sequenced through Sanger sequencing. On the basis of PCR results, four similar amplified products from both regions were obtained showing similarities in agarose gel electrophoresis, but they differ from each other on the basis of nucleotides sequence. Phylogenetic analysis revealed that nucleotide sequences of isolates from Karachi were similar to the IBV H-120, Mass-41 and Connecticut 46 reference strains. Whereas, isolates from the Punjab province are analogous to the Mans-2, Mans-3, 9/41(UK) but did not show significant similarity with other reference strain. Therefore, it is recommended that use of M-41 and H-120 in vaccine production could be effective measure against velogenic infectious agent in Sindh particularly in Karachi, whereas, it would be better to incorporate either of the variant GQ281656.1, AY279533.1 in vaccine because of their highest level of resemblance with genetically sequenced isolates from Lahore a

关键词： Infectious Bronchitis Virus S1 Glycoprotein Polymerase Chain Reaction Virus Neutralization Test Molecular Evolutionary Genetics Analysis

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Herbal medicine in treatment of dysmenorrhea:a systematic review

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TMR Integrative Medicine 2022年第23期6卷 1-7页

作者： Hamidreza Sheikh Roshande Romina Negari Namaghi Fatemeh Ghadimi Zohreh Pour ahmad sajjad Ghasemzadeh Neda Moghari Zadeh Roksana Mirkazemi Farzanegan Nik Andish Institute for the Development of Knowledge and Technology TehranIran. Research Institute of Nutritional Sciences and Traditional Medicine Ta'm Asrar TehranIran. Faculty of Nutrition Tabriz University of Medical ScienceTabrizIran. Department of Cultural Sociology Faculty of HumanitiesIslamic Azad University Kish InternationalKish IslandIran. Emam Hossein Hospital Iran Medical Science UniversityTehranIran.

Objective:Herbal medicine has been a standard treatment for primary dysmenorrhea in different cultures and countries,and multiple studies have shown its possible positive effect on pain *** systematic review of the literature was conducted to assess different types of herbal medicine used to treat primary dysmenorrhea and evaluate the effectiveness of these ***:This study systematically reviewed published articles on herbal medicine treating primary dysmenorrhea for 10 years(2011-2021),using Preferred Reporting Items for Systematic Reviews and Meta-Analyses(PRISMA)*** February 2022,international credible scholarly databases were ***,the references of selected articles were hand searched to find relevant *** on the search syntax,the search guideline was first defined for the PubMed database and was later revised according to each database’s specific framework of search *** studies were included if they were either clinical trials or Quasi-Experimental,published in English or Persian between 2011 to *** literature,unfinished studies,and studies without well-defined primary dysmenorrheal participants were ***:Seventy-three studies investigated the effectiveness of a total of 44 plants and plant combinations(35 single plants and 9 combinations of plants)on primary dysmenorrhea of which only one study did not report a significant positive *** was the most investigated *** studies assessed the effects of ginger alone,and four studied the combination of ginger with other plants,all of them showed significant effects but one *** studies investigated the effects of either fennel or its combination,five studies were on chamomile and three studies were related to ***,all of them,except one,reported the positive effect of herbal medicine on primary ***:Different herbal medicine has been used to treat primary dysmenorrhea,significantly reducing

关键词： primary dysmenorrhea herbal medicine plant plant extracts clinical trial systematic review

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A Potential Approach to Enhance the Seebeck Coefficient of UHMWPE by Using the Graphene Oxide

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Non-Metallic Material Science 2020年第2期2卷 21-27页

作者： Aqsa Irfan Malik Naqash Mehmood Malik sajjad Mehmood Arif Aziz Mansoor ahmad Baluch Muhammad Rizwan Tariq Yasin Department of Basic Science University of Engineering and TechnologyTaxila47050Pakistan Department of Physics Air University444000IslamabadPakistan Harbin Engineering University HarbinHeilingjiang150001China Department of Chemistry Pakistan Institute of Engineering and Applied Sciences45650IslamabadPakistan

Thermoelectric materials have been a competent source for the production of energy in the present *** most important and potential parameter required for the material to have better thermoelectric characteristics is the Seebeck *** this work,ultra high molecular weight polyethylene(UHMWPE)and graphene oxide(GO)nanocomposites were prepared by mechanical mixing by containing 10000ppm,50000ppm,70000ppm,100000ppm,150000ppm,and 200000ppm loadings of graphene *** to the intrinsic insulating nature of UHMWPE,the value of Seebeck for pristine UHMWPE and its nanocomposites with 10000ppm&50000ppm of GO concentration was too low to be ***,the Seebeck coefficient for composites with 70000ppm,100000ppm,150000ppm,and 200000ppm loadings of GO was found to be 180,206,230,and 235μV/K,*** higher values of Seebeck coefficients were attributed to the superior thermal insulating nature of UHMWPE and the conductive network induced by the GO within the UHMWPE insulating ***,the values of the figure of merit and power factor were negligibly small due to the lower concentration of charge carriers in UHMWPE/GO nanocomposites but still reported,results are extremely hopeful for considering the composite as the potential candidate for thermoelectric applications.

关键词： UHMWPE Graphene oxide Electrical conductivity Thermal conductivity Seebeck coefficient Figure of merit

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Effects of Humic Acid and Crop Residues on Soil and Wheat Nitrogen Contents

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American Journal of Plant Sciences 2014年第9期5卷 1277-1284页

作者： Kashif Akhtar Syed Noor Muhammad Shah Amjad Ali sajjad Zaheer Fazli Wahid ahmad Khan Mohib Shah Shahida Bibi Abdul Majid College of Natural Resources and Environment Northwest A&F University Yangling China Department of Agriculture Chemistry Faculty of Agriculture Gomal University D.I. Khan Pakistan Department of Agronomy Amir Muhammad Khan Campus Mardan University of Agriculture Peshawar Pakistan Department of Agronomy Faculty of Crop Production Sciences University of Agriculture Peshawar Pakistan Department of Botany Abdul Wali Khan University Mardan Pakistan Department of Horticulture Faculty of Agriculture Gomal University D.I. Khan Pakistan Department of Weed Science University of Agriculture Peshawar Pakistan Soil and Environmental Sciences University of Agriculture Peshawar Pakistan

This study was conducted with the aim of finding humic acid and crop residues effects on soil and wheat nitrogen at New Development Farm, University of Agriculture, Peshawar, Pakistan during winter 2009-2010. The experiment was laid out in RCB design having four replications. Different levels (1, 1.5, 2 and 2.5 kg ha-1) of Humic acid were applied at sowing time. Mungbean (5 tons ha-1), wheat straw (10 tons ha-1) and mungbean (2.5 tons ha-1) + wheat straw (5 tons ha-1) were incorporated 30 days before sowing. Our results showed higher mineral and total nitrogen (7.32 and 0.43, respectively) mg kg-1 in soil and also higher nitrogen in stem, leaves and grains (2.3, 4.6 and 21.1, respectively) mg kg-1 at maturity in those plots in which 2.5 kg ha-1 of humic acid was applied. Higher mineral and total nitrogen (7.04 and 0.5, respectively) mg kg-1 in soil and also higher nitrogen in leaves (5.5) mg kg-1 at pre-anthesis, while at maturity stage in stem, leaves and grain (5.5, 2.1, 4.2 and 20.8) g kg-1 was recorded in those plots in which 5 tons ha-1 mung bean was incorporated. Our experimental results suggest the use of 5 tons ha-1 of mungbean residues with 2.5 kg ha-1 of humic acid to improve soil fertility availability of more nitrogen in wheat plants to increase the crop yield and grains quality.

关键词： Anthesis Grain Maturity Soil Fertility Straw Mung Bean

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