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Chemotransformation of Bacterial Cells Without Heat-shock

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Chemical Research in Chinese Universities 2017年第2期33卷 160-165页

作者： wang sainan LI Zhenhua CHEN Zhijun State Key Laboratory of Supramolecular Structure and Materials Institute of Theoretical Chemistry International Joint Research Laboratory of Nano-micro Architecture Chemistry Jilin University Changchun 130012 R R. China

Transformation of DNA into microbial cells via heat-shock approach has been well established in the fieldof molecular biology for decades. Herein we described an unexpected finding that heat-shock may not play an essen-tial role in the transformation process. This observation was verified via UV-Vis and fluorescence spectroscopies, andconfocal microscopy images for various DNAs and bacterial cells. The non-heat-shock approach proposed in thisstudy can be a convenient and beneficial modification for DNA transformation, especially for those laboratorieslacking ice machine and heat-shock equipment.

关键词： Heat-shock Chemotransformation DNA Bacteria

Robust Secrecy Transmission for Two-tier Femtocell Networks under Probability Constraint 36

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Robust Secrecy Transmission for Two-tier Femtocell Networks ...

Cdc42 deletion yielded enamel defects by disrupting mitochondria and producing reactive oxygen species in dental epithelium

第36届中国控制会议

作者： Zhixin Liu sainan wang Peng Zhang Kai Ma Xinbin Li Institute of Electrical Engineering Yanshan University

ISBN: (纸本)9781538629185

A secrecy transmission method with robust power control is investigated in this paper for a downlink two-tier femtocell network,where an eavesdropper attempts to wiretap the legitimate macrocell *** the imperfect channel gains,a probability constraint robust optimization problem is formulated to satisfy the quality-of-service（QoS） of *** aim to maximize the secrecy rate with the constrains of power outage probability and limited power at base stations（BSs）.Firstly,the problem of power outage probability is solved by a novel conversion ***,the object function is a non-convex.A simple method based on variable substitution and Taylor expansion is presented to solve this puzzle in this ***,the iterative algorithm is provided to get the practical operation for ***,numerical simulation results are given to illustrate the effectiveness of the proposed algorithm.

关键词： Femtocell Power control Robust optimization Probability constraint Secrecy transmission

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Genes & Diseases 2024年第5期11卷 335-349页

作者： Jinxuan Zheng Rongcheng Yu Yiqi Tang Sihui Su sainan wang Chenxi Liao Xuecong Li Jiabin Liao Dongsheng Yu Tingting Ai Wei Zhao Vicky Yau Chufeng Liu Liping Wu Yang Cao Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhouGuangdong 510055China Guangdong Provincial Key Laboratory of Oral Diseases GuangzhouGuangdong 510055China Department of Cariology and Endodontology Peking University School and Hospital of Stomatology&National Center for Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Research Center of Oral Biomaterials and Digital Medical DevicesBeijing 100081China Department of Oral and Maxillofacial Surgery University at BuffaloBuffaloNY 14214USA Department of Orthodontics Stomatological HospitalSouthern Medical UniversityGuangzhouGuangdong 510280China

Developmental defects of enamel are common due to genetic and environmental factors before and after ***42,a Rho family small GTPase,regulates prenatal tooth development in ***,its role in postnatal tooth development,especially enamel formation,remains ***,we investigated Cdc42 functions in mouse enamel development and tooth repair after ***42 showed highly dynamic temporospatial patterns in the developing incisors,with robust expression in ameloblast and odontoblast ***,epithelium-specific Cdc42 deletion resulted in enamel defects in *** differentiation was inhibited,and hypomineralization of enamel was observed upon epithelial Cdc42 *** analysis showed that abnormal mitochondrial components,phosphotransferase activity,and ion channel regulator activity occurred in the Cdc42 mutant dental *** oxygen species accumulation was detected in the mutant mice,suggesting that abnormal oxidative stress occurred after Cdc42 ***,Cdc42 mutant mice showed delayed tooth repair and generated less calcified *** dysfunction and abnormal oxygen consumption were evidenced by reduced Apool and Timm8a1 expression,increased Atp5j2 levels,and reactive oxygen species overproduction in the mutant repair ***-specific Cdc42 deletion attenuated ERK1/2 signaling in the labial cervical *** Sox2 expression in the mutant labial cervical loop after clipping might lead to delayed tooth *** findings suggested that mitochondrial dysfunction,up-regulated oxidative stress,and abnormal ion channel activity may be among multiple factors responsible for the observed enamel defects in Cdc42 mutant ***,Cdc42 exerts multidimensional and pivotal roles in enamel development and is particularly required for ameloblast differentiation and enamel matrix formation.

关键词： Cdc42 Enamel defects Mitochondrial dysfunction Rho GTPase Tooth repair

Alkaline activation of endogenous latent TGFβ1 by an injectable hydrogel directs cell homing for in situ complex tissue regeneration

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Bioactive Materials 2022年第9期7卷 316-329页

作者： sainan wang Yuting Niu Peipei Jia Zheting Liao Weimin Guo Rodrigo Cotrim Chaves Khanh-Hoa Tran-Ba Ling He Hanying Bai Sam Sia Laura J.Kaufman Xiaoyan wang Yongsheng Zhou Yanmei Dong Jeremy J.Mao Department of Cariology and Endodontology Peking University School and Hospital of StomatologyBeijing100081China Department of Prosthodontics Peking University School and Hospital of StomatologyBeijing100081China Center for Craniofacial Regeneration Columbia UniversityNew YorkNY10032USA Department of Biomedical Engineering Columbia UniversityNew YorkNY10027USA Department of Chemistry Columbia UniversityNew YorkNY10027USA

Utilization of the body’s regenerative potential for tissue repair is known as in situ tissue ***,the use of exogenous growth factors requires delicate control of the dose and delivery strategies and may be accompanied by safety,efficacy and cost *** this study,we developed,for the first time,a biomaterial-based strategy to activate endogenous transforming growth factor beta 1(TGFβ1)under alkaline conditions for effective in situ tissue *** demonstrated that alkaline-activated TGFβ1 from blood serum,bone marrow fluids and soaking solutions of meniscus and tooth dentin was capable of increasing cell recruitment and early differentiation,implying its broad ***,we engineered an injectable hydrogel(MS-Gel)consisting of gelatin microspheres for loading strong alkaline substances and a modified gelatin matrix for hydrogel click *** vitro models showed that alkaline MS-Gel controllably and sustainably activated endogenous TGFβ1 from tooth dentin for robust bone marrow stem cell *** importantly,infusion of in vivo porcine prepared root canals with alkaline MS-Gel promoted significant pulp-dentin regeneration with neurovascular stroma and mineralized tissue by endogenous proliferative ***,this work offers a new bench-to-beside translation strategy using biomaterial-activated endogenous biomolecules to achieve in situ tissue regeneration without the need for cell or protein delivery.

关键词： Endogenous TGFβ1 Injectable alkaline hydrogel Cell homing Pulp-dentin complex In situ tissue regeneration

Expression and Purification of Human Coagulation Factor X in Mammalian CHO-DG44 Cells

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Agricultural Biotechnology 2023年第3期12卷 50-54页

作者： Jinwu CHEN Yi LI Mei LIU sainan wang Zilong XIAO Junjie XIA Lulu QI School of Biology and Food Engineering Hefei Normal UniversityHefei 230601China School of Life Sciences Anhui UniversityHefei 230039China

[Objectives]This study was conducted to obtain a Chinese hamster ovary cell line that stably expresses recombinant human coagulation factor X(rhFX),and to induce efficient expression of the target gene with different concentrations of methotrexate(MTX).[Methods]PCR was performed to obtain the rhFX gene,and a recombinant expression plasmid pOptiVEC-rhFX was constructed and subjected to double restriction endonuclease digestion and sequencing ***-DG44(DHFR-)cells were transfected by the liposome method,and the target protein was purified by affinity chromatography and detected by SDS-PAGE electrophoresis and Western blot.A cell line with efficient and stable expression of the target gene was obtained by increasing the concentration of MTX to select positive clones.[Results]PCR yielded a 1509 bp rhFX sequence,and the results of double digestion and sequencing showed that the constructed pOptiVEC-rhFX plasmid was *** transfection of cells,MTX significantly increased protein *** MTX reached 1.0μmol/L,the expression efficiency of the target protein was(9±0.27)μg/*** purity of the target protein purified by affinity chromatography was 93%,which could be used for subsequent *** expression efficiency of rhFX in eukaryotic mammalian cells was improved by increasing MTX concentration,and an affinity chromatography purification process for the target protein was preliminarily established.[Conclusions]The results of this study provide data support for the expression and purification of rhFX,and will lay a solid foundation for the development of drugs related to rhFX.

关键词： Recombinant human coagulation factor X(rhFX) Eukaryotic expression MTX Affinity chromatography

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基于粒子滤波与样本加权的压缩跟踪算法

电子与信息学报 2018年第6期40卷 1397-1403页

作者：张红颖王赛男胡文博中国民航大学电子信息与自动化学院天津300300

该文针对压缩跟踪算法无法适应目标尺度的变化以及没有考虑样本权重的问题,提出一种基于粒子滤波与样本加权的压缩跟踪算法。首先,对压缩特征进行改进,提取归一化矩形特征用于构建目标表观模型。然后,引入样本加权的思想,根据正样本与... 详细信息

该文针对压缩跟踪算法无法适应目标尺度的变化以及没有考虑样本权重的问题,提出一种基于粒子滤波与样本加权的压缩跟踪算法。首先,对压缩特征进行改进,提取归一化矩形特征用于构建目标表观模型。然后,引入样本加权的思想,根据正样本与目标之间距离的不同赋予正样本不同的权重,提高分类器的分类精度。最后,在粒子滤波的框架下融合尺度不变压缩特征进行动态状态估计,在粒子预测阶段利用2阶自回归模型对粒子状态进行估计与预测,借助观测模型对粒子状态进行更新,并且对粒子进行重采样以防止粒子退化。实验结果表明,相比于原始压缩跟踪算法,改进算法能够更好地跟踪目标尺度的变化,提高跟踪的稳定性和准确性。

关键词：压缩跟踪粒子滤波样本加权分类器

无线通信中空间调制MIMO技术的研究现状与展望

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数据采集与处理 2017年第3期32卷 440-453页

作者：虞湘宾邱赛男王丞南京航空航天大学电子信息工程学院南京210016

空间调制(Spatial modulation,SM)技术借助于天线序号"隐形"地传输信息,能够获得高数据传输速率,而且可有效克服信道间的干扰和同步问题,降低系统的实现复杂度。多输入多输出(Multiple input multiple output,MIMO)技术,能够极大地提高... 详细信息

空间调制(Spatial modulation,SM)技术借助于天线序号"隐形"地传输信息,能够获得高数据传输速率,而且可有效克服信道间的干扰和同步问题,降低系统的实现复杂度。多输入多输出(Multiple input multiple output,MIMO)技术,能够极大地提高无线通信系统的容量和频谱效率。为了满足无线通信系统高质量和高速率的要求,将SM和MIMO技术有效结合,SM-MIMO技术应运而生,成为近年来无线通信领域研究的热点方向。本文从基本原理和性能分析两方面介绍了SM-MIMO技术及其研究现状,并且探讨了SM-MIMO技术的应用前景。同时也对近年来国内外研究团队在SM-MIMO方面的研究成果进行了综述和概括,最后对该领域的未来研究工作进行了分析和展望。

关键词：空间调制多输入多输出容量误比特率广义空间调制空移键控

甲基丙烯酰明胶水凝胶作为细胞三维培养支架在骨组织工程中的应用

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中国组织工程研究 2024年第22期28卷 3576-3582页

作者：王赛楠王晓菲张莉滨州医学院山东省滨州市256600 滨州医学院附属医院口腔科山东省滨州市256600

背景:骨缺损的治疗一直是临床医生亟待解决的临床难题,用甲基丙烯酰明胶进行细胞体外3D培养,可以为大面积骨缺损的治疗提供新的方向。目的:文章综述了甲基丙烯酰明胶作为3D细胞培养支架在骨组织工程中的研究进展,以期为临床骨缺损修复... 详细信息

背景:骨缺损的治疗一直是临床医生亟待解决的临床难题,用甲基丙烯酰明胶进行细胞体外3D培养,可以为大面积骨缺损的治疗提供新的方向。目的:文章综述了甲基丙烯酰明胶作为3D细胞培养支架在骨组织工程中的研究进展,以期为临床骨缺损修复提供进一步的参考。方法:应用计算机检索中国知网及PubMed数据库1986年1月至2023年8月收录的文献,中英文检索词分别为“骨缺损,骨组织工程,生物支架材料,水凝胶,光交联水凝胶,甲基丙烯酰明胶,三维培养,细胞培养”和“Bone defect,Bone tissue engineering,Biomaterial scaffold,Hydrogel,Methylacrylyl photocrosslinked hydrogel,Three-dimensional culture;Cell culture”,最终纳入68篇文献进行综述分析。结果与结论:①同二维培养相比,3D培养可以在无菌条件下,构建立体三维空间,更好地模拟体内环境,为细胞提供合适的温度、酸碱度及足够的营养,使细胞能够在体外正常生长增殖并保持其正常结构与功能,具有独特优势。②在骨组织工程生物支架材料的选择中,水凝胶因其良好的生物相容性、可降解性及具有立体三维网络结构等优点,已被广泛应用于骨再生的研究。③甲基丙烯酰明胶的物理及生物性能受到浓度、光照时间及光引发剂种类以及反应体系等因素的影响,而这些性能均能对细胞的黏附、生长及增殖,甚至细胞形态及功能产生一定的影响。④甲基丙烯酰明胶因具有良好的生物相容性、物理可调节性、可注射性及光敏性能,已被广泛应用于3D细胞封装、3D生物打印及基于数字光处理的立体光刻技术等细胞3D培养体系中。⑤应用各类复合甲基丙烯酰明胶进行细胞的3D培养,可以更好地促进血管形成及骨再生,为临床骨缺损的治疗提供更多可能。⑥目前甲基丙烯酰明胶的来源、合成的方法以及安全性尚没有健全的标准,需要进一步加强研究,对甲基丙烯酰明胶在3D细胞培养领域的应用进行更深入的完善。

关键词：骨缺损骨组织工程生物支架材料水凝胶光交联水凝胶甲基丙烯酰明胶二维培养三维培养细胞培养

CNTs@Y2O3:Eu^3+纳米复合材料的制备及其荧光温敏性能

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功能材料 2020年第6期51卷 6195-6199页

作者：王赛楠李颖张春明上海理工大学材料与科学工程学院上海200093

采用表面活性剂(十二烷基苯磺酸钠)辅助的均相沉淀法,将稀土荧光前驱体氧化物复合在具有一维结构的碳纳米管表面,成功制备了具有一维结构的CNTs@Y2O3:Eu3+纳米复合材料。通过SEM、XRD、TEM-EDS、FT-IR、TGA和PL等表征手段分别探究了复... 详细信息

采用表面活性剂(十二烷基苯磺酸钠)辅助的均相沉淀法,将稀土荧光前驱体氧化物复合在具有一维结构的碳纳米管表面,成功制备了具有一维结构的CNTs@Y2O3:Eu3+纳米复合材料。通过SEM、XRD、TEM-EDS、FT-IR、TGA和PL等表征手段分别探究了复合材料的微观形貌结构、热稳定性、结晶度以及荧光性能。研究结果表明,CNTs@Y2O3:Eu3+荧光复合材料在UV激发下显示出强烈的稀土Eu3+特征荧光。同时,在400~1000℃不同煅烧温度范围内,该复合材料荧光强度随之发生明显变化,具有较强的温度敏感特性。

关键词： CNTs Y2O3:Eu^3+ 均相沉淀法荧光温敏性能