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Glycogen synthase kinase 3: a crucial regulator of axotomy-induced axon regeneration

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Neural Regeneration Research 2020年第5期15卷 859-860页

作者： Jinlian Liu Qing Zhou Chaoqun Liu Chunfeng Liu saijilafu Department of Orthopedic Surgery Suzhou Kowloon HospitalShanghai Jiaotong University Medical SchoolSuzhouJiangsu ProvinceChina Orthopedic Institute Medical CollegeSoochow UniversitySuzhouJiangsu ProvinceChina Department of Orthopedic Surgery the First Affiliated HospitalSoochow UniversitySuzhouJiangsu ProvinceChina

Following nerve injury,axonal disconnection in neurons usually results in persistent functional deficits,such as ***,axons in the adult mammalian central nervous system (CNS) have very limited regenerative *** the molecular mechanism of controlling axon regeneration can provide idea for the design of effective therapeutic interventions for CNS injury,such as spinal cord *** axonal regeneration is achieved via gene expression in the neuronal soma,axonal transport of raw materials along the shaft,and membrane and cytoskeleton assembly at the nerve growth *** process is delicately regulated by spatial-temporal controlled signaling pathways that target distinct *** expression in the neuronal soma,especially of transcription factors,is often activated immediately following nerve *** signals at distal axons are interpreted and transmitted back to the soma,initiating a stream of gene expression events which positively regulate subsequent axonal *** the past few decades,extensive studies have identified many regeneration-associated genes,including CREB,nuclear factor of activated T-cells,protein 53,Sprr1a,c-Jun,Smad1,activating transcription factor 3,signal transducer and activator of transcription 3,SRF,Sox11,and Kruppel-like ***,we know far less about how the coordinated expression of these regeneration-associated genes is regulated during axonal ***,it is possible that they are regulated by a single common upstream *** so,identification of this upstream regulator will provide us with an invaluable target for the development of more effective treatments for traumatic nerve *** dorsal root ganglion (DRG) neurons represent a favorable medium in which to study the molecular mechanisms controlling intrinsic neuronal axon growth *** of the peripheral branch of a DRG neuron,known as a “conditioning lesion”,has been well-documented t

关键词： gene expression spinal cord central nervous system

Decoding epigenetic codes: new frontiers in exploring recovery from spinal cord injury

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Neural Regeneration Research 2020年第9期15卷 1613-1622页

作者： Bo-Yin Zhang Peng-Yu Chang Qing-San Zhu Yu-Hang Zhu saijilafu Department of Orthopedic Surgery China-Japan Union Hospital of Jilin UniversityChangchunJilin ProvinceChina Department of Radiotherapy The First Bethune Hospital of Jilin UniversityChangchunJilin ProvinceChina Department of Orthopedics The First Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina

Spinal cord injury that results in severe neurological disability is often *** poor clinical outcome of spinal cord injury is mainly caused by the failure to reconstruct the injured neural *** intrinsic and extrinsic determinants contribute to this inability to *** regulation acts as the driving force for multiple pathological and physiological processes in the central nervous system by modulating the expression of certain critical *** studies have demonstrated that post-SCI alteration of epigenetic landmarks is strongly associated with axon regeneration,glial activation and *** findings not only establish a theoretical foundation for further exploration of spinal cord injury,but also provide new avenues for the clinical treatment of spinal cord *** review focuses on the epigenetic regulation in axon regeneration and secondary spinal cord ***,these discoveries are a selection of epigenetic-based prognosis biomarkers and attractive therapeutic targets in the treatment of spinal cord injury.

关键词： axon regeneration epigenetic biomarkers epigenetic modification histone microRNA prognosis secondary injury spinal cord injury

环黄芪醇促进脊髓损伤小鼠运动功能恢复及皮质神经元再生的相关机制

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中国组织工程研究 2025年第20期29卷 4258-4265页

作者：陶紫晗赛吉拉夫苏州大学苏州医学院骨科研究所江苏省苏州市215031

背景:前期研究证实,环黄芪醇能够促进坐骨神经损伤修复,且环黄芪醇能够激活端粒酶反转录酶进一步参与外周感觉神经元轴突再生的调节。但环黄芪醇是否可以激活皮质神经元轴突再生仍然未知,尤其是环黄芪醇是否参与脊髓损伤修复过程中运动... 详细信息

背景:前期研究证实,环黄芪醇能够促进坐骨神经损伤修复,且环黄芪醇能够激活端粒酶反转录酶进一步参与外周感觉神经元轴突再生的调节。但环黄芪醇是否可以激活皮质神经元轴突再生仍然未知,尤其是环黄芪醇是否参与脊髓损伤修复过程中运动功能的恢复。目的:探究环黄芪醇对小鼠脊髓损伤后运动功能恢复的影响,以及环黄芪醇促进皮质神经元再生的相关机制。方法:①将12只ICR小鼠随机分为溶剂组、环黄芪醇组,每组6只,构建脊髓损伤模型后分别腹腔注射含2%吐温20和5%二甲基亚砜的PBS、环黄芪醇(20 mg/kg),连续给药12周,使用BMS运动功能评分评估小鼠肢体运动功能;②体外培养E14胎鼠大脑皮质神经元,分为二甲基亚砜组与环黄芪醇组,分别用二甲基亚砜、0.5μmol/L环黄芪醇体外培养3 d;③E7孕鼠分为溶剂组与环黄芪醇组,分别腹腔注射含2%吐温20和5%二甲基亚砜的PBS、20 mg/kg环黄芪醇,连续给药7 d,分离出胎鼠大脑皮质,体外培养皮质神经元3 d;④对胎鼠大脑皮质神经元进行Tuj1免疫荧光染色,分析Tuj1阳性细胞的轴突长度,验证环黄芪醇对皮质神经元轴突再生的作用;对胎鼠大脑皮质神经元进行Tuj1、端粒酶反转录酶双标免疫荧光染色,分析环黄芪醇对端粒酶反转录酶表达的影响;蛋白免疫印迹分析胎鼠大脑皮质神经元中端粒酶反转录酶蛋白的相对表达;⑤将6只ICR小鼠随机分为溶剂组、环黄芪醇组,每组3只,构建脊髓损伤模型后分别腹腔注射含2%吐温20和5%二甲基亚砜的PBS和环黄芪醇(20 mg/kg),连续给药7 d,蛋白免疫印迹检测脊髓组织中端粒酶反转录酶蛋白的相对表达。结果与结论:①脊髓损伤后环黄芪醇治疗4,6,8,12周,环黄芪醇组小鼠运动功能评分显著高于溶剂组(P<0.0001);脊髓损伤后环黄芪醇治疗7 d,环黄芪醇组小鼠脊髓组织中端粒酶反转录酶蛋白相对表达量显著高于溶剂组(P<0.05);②Tuj1免疫荧光染色结果显示,0.5μmol/L环黄芪醇组体外干预的皮质神经元轴突长度显著大于二甲基亚砜组(P<0.001),腹腔注射20 mg/kg环黄芪醇组的皮质神经元轴突长度显著大于溶剂组(P<0.01);③端粒酶反转录酶免疫荧光染色结果显示,0.5μmol/L环黄芪醇组体外干预的皮质神经元的端粒酶反转录酶荧光强度显著大于二甲基亚砜组(P<0.01);④蛋白免疫印迹检测显示,0.5μmol/L环黄芪醇组体外干预的皮质神经元中端粒酶反转录酶蛋白相对表达量显著高于二甲基亚砜组(P<0.05),腹腔注射20 mg/kg环黄芪醇组的皮质神经元中端粒酶反转录酶蛋白相对表达量显著高于溶剂组(P<0.05)。结果表明,环黄芪醇能够促进皮质神经元轴突生长并改善小鼠脊髓损伤后运动功能,这一现象可能与端粒酶反转录酶的表达升高有关。

关键词：环黄芪醇皮质神经元轴突生长端粒酶反转录酶运动功能脊髓损伤工程化动物模型

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Multiple stress fractures of unilateral femur:A case report

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World Journal of Clinical Cases 2022年第13期10卷 4280-4287页

作者： Mao-Ting Tang Chun-Feng Liu Jin-Lian Liu saijilafu Zhen Wang Department of Orthopaedics Suzhou Kowloon Hospital Shanghai Jiao Tong University School of MedicineSuzhou 215028Jiangsu ProvinceChina Orthopaedic Institute Medical CollegeSoochow UniversitySuzhou 215007Jiangsu ProvinceChina

BACKGROUND Stress fractures of the femoral neck are not common in clinical practice,and simultaneous stress fractures of the femoral neck and proximal femur of the unilateral femur are even more *** introduce a case of this type of fracture that was treated in our department,analyze the causes,and review similar stress fractures reported in the literature to provide references for the diagnosis and treatment of such *** SUMMARY A 62-year-old female,with a free medical history,was admitted to the hospital mainly due to pain in the right hip and worsening pain in the right *** patient had no obvious history of trauma.X-ray and computed tomography showed fracture of the femoral neck and proximal *** patient had undergone surgery 1 year prior to address a fracture of the left proximal femur that had occurred in a traffic *** first consideration was stress fracture of the femoral neck;however,simultaneous stress fractures of the femoral neck and proximal femur of the unilateral femur were *** femoral neck stress fracture was a tension fracture,with obvious displacement and varus deformity of the *** that the patient was an elderly female,we performed total hip ***-up X-rays showed that the stress fracture of the proximal femur had mostly healed after 3 *** Muscle fatigue and hip varus deformity provide an anatomical basis for the occurrence of femoral neck stress fractures.

关键词： Femoral neck fracture Femoral fracture Stress fracture Fatigue fracture Hip pain Case report

Local manipulation of the growth cone cytoskeleton:new strategies to promote axon regeneration

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Neural Regeneration Research 2015年第12期10卷 1959-1960页

作者： Zi-wei Huang Feng-quan Zhou saijilafu Department of Orthopedics the First Affiliated HospitalOrthopedic InstituteSoochow University Department of Orthopedic Surgery and the Solomon H.Snyder Department of Neuroscience the Johns Hopkins University School of Medicine

Thousands of spinal cord injuries occur worldwide as a result of traumatic or non-traumatic *** cord injury usually results in axonal damage,leading to permanent functional deficits and ***,axonal regeneration is the ... 详细信息

关键词： CSPG OMgp CNS

Heel pain caused by os subcalcis:A case report

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World Journal of Clinical Cases 2022年第16期10卷 5373-5379页

作者： saijilafu Suo-Yuan Li Xiao Yu Zhi-Qiang Li Guang Yang Jing-Huan Lv Guang-Xiang Chen Ren-Jie Xu Department of Orthopaedics Soochow UniversitySuzhou 215007Jiangsu ProvinceChina Department of Orthopaedics the Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou Municipal HospitalSuzhou 215000Jiangsu ProvinceChina Department of pathology the Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou Municipal HospitalSuzhou 215000Jiangsu ProvinceChina

BACKGROUND The accessory bones are common bone variations around the feet and ankles,which usually originate from nonunion of the secondary ossification center adjacent to the main bone mass,and most of them remain *** subcalcis is an accessory bone at the plantar aspect of the calcaneus,which is located just posterior to the insertion of the plantar *** bone formation at the calcaneal plantar pole with heel pain has rarely been *** SUMMARY A 55-year-old man presented to our clinic with left plantar heel pain and a progressive swelling for 8 years.X-ray,computer tomography and magnetic resonance imaging showed a large os subcalcison the plantar side of the calcaneus,located at the insertion of the plantar *** underwent surgical excision of the *** the bony trabeculae were intermingled with fat and covered with *** This is a rare case with accessory os subcalcis leading to heel *** highlights the awareness of os subcalcis and helps avoid future misdiagnosis of heel pain.

关键词： Heel pain Accessory ossicle Calcaneus Os subcalcis Case report

Deletion of Krüppel-like factor-4 promotes axonal regeneration in mammals

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Neural Regeneration Research 2021年第1期16卷 166-171页

作者： Jin-Hui Xu Xu-Zhen Qin Hao-Nan Zhang Yan-Xia Ma Shi-Bin Qi Hong-Cheng Zhang Jin-Jin Ma Xin-Ya Fu Ji-Le Xie saijilafu Department of Orthopedics The First Affiliated Hospital of Soochow UniversityOrthopedic InstituteSoochow UniversitySuzhouJiangsu ProvinceChina

Axonal regeneration plays an important role in functional recovery after nervous system ***,after axonal injury in mammals,regeneration is often *** deletion of Krüppel-like factor-4(Klf4)has been shown to promote axonal regeneration in retinal ganglion ***,the effects of Klf4 deletion on the corticospinal tract and peripheral nervous system are *** this study,using a mouse model of sciatic nerve injury,we show that the expression of Klf4 in dorsal root ganglion sensory neurons was significantly reduced after peripheral axotomy,suggesting that the regeneration of the sciatic nerve is associated with *** vitro,dorsal root ganglion sensory neurons with Klf4 knockout exhibited significantly enhanced axonal ***,the regeneration of the sciatic nerve was enhanced in vivo following Klf4 ***,AAV-Cre virus was used to knockout the Klf4 gene in the *** deletion of Klf4 enhanced regeneration of the corticospinal tract in mice with spinal cord ***,our findings suggest that regulating KLF4 activity in neurons is a potential strategy for promoting axonal regeneration and functional recovery after nervous system *** study was approved by the Animal Ethics Committee at Soochow University,China(approval ***20200316A01).

关键词： axonal regeneration corticospinal tract dorsal root ganglion Klf4 peripheral nervous system sciatic nerve crush sensorimotor cortex spinal cord crushl

Individualized vaginal applicator for stage IIb primary vaginal adenocarcinoma:A case report

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World Journal of Clinical Oncology 2024年第8期15卷 1102-1109页

作者： saijilafu Yan-Jun Gu Ai-Wu Huang Chang-Fen Xu Li-Wen Qian Hangzhou Lin'an Traditional Chinese Medicine Hospital Affiliated HospitalHangzhou City UniversityHangzhou 311300Zhejiang ProvinceChina Department of Radiation Oncology Sir Run Run Shaw HospitalHangzhou 310016Zhejiang ProvinceChina Department of the Gynecology and Obstetrics Hangzhou Lin'an Traditional Chinese Medicine HospitalAffiliated HospitalHangzhou City UniversityHangzhou 311300Zhejiang ProvinceChina

BACKGROUND Primary vaginal cancer is rare and most vaginal tumors are metastatic,often arising from adjacent gynecologic *** vaginal cancers are also more common among postmenopausal women and most of these are squamous cell carcinomas,with adenocarcinomas being relatively *** bleeding is the most common clinical manifestation of vaginal *** 70%of vaginal adenocarcinomas are stage I lesions at the time of diagnosis,for which radical surgery is ***,more advanced vaginal cancers are not amenable to radical surgical treatment and have poor clinical *** treatments modes are still being ***,we report a rare case of stage IIb primary vaginal adenocarcinoma for which an individually designed vaginal applicator for after-loading radiotherapy was used to achieve good tumor *** SUMMARY A 62-year-old woman presented to our clinic after 3 months of abnormal postmenopausal vaginal *** examination,computed tomography(CT),and positron emission tomography-CT showed a large mass(about 5 cm)on the anterior vaginal *** biopsy confirmed adenocarcinoma of vaginal *** three cycles of carboplatin plus paclitaxel chemotherapy,the lesion partially *** patient then received external irradiation of 45 gray(gy)in 25 fractions,which further reduced the vaginal lesion,followed by after-loading radiotherapy of 30 gy in 5 fractions with an individually designed vaginal *** months later,magnetic resonance imaging showed a slight thickening CONCLUSION Primary vaginal adenocarcinoma is rare,and prognosis is poor in most vaginal cancers of locally advanced stages,which cannot be treated with radical *** tumor control can be achieved with an individualized vaginal applicator that allows administration of a higher radical dose to the tumor area while protecting normal tissues.

关键词： Primary vaginal adenocarcinoma After-loading radiotherapy Vaginal applicator Tumor control Case report

Percutaneous kyphoplasty in the treatment of Kümmell disease in lumbar scoliosis:A case report

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World Journal of Clinical Cases 2024年第17期12卷 3123-3129页

作者： saijilafu Jia-Wen Zhou Gen-Lin Wang Ke-Hong Sun Ji-Le Xie Department of Orthopaedic Surgery Hangzhou Lin’an Traditional Chinese Medicine HospitalAffiliated HospitalHangzhou City UniversityHangzhou 311300Zhejiang ProvinceChina Department of Orthopaedic Surgery The First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina

BACKGROUND Due to mechanical imbalance in the spine,elderly scoliosis patients tend to develop vertebral fracture nonunion,i.e.,Kümmell disease,when osteoporotic vertebral compression fractures ***,accompanying vertebral rotational deformities make surgical procedures challenging *** patients are usually compelled to undergo conservative treatment and there are very few reports on minimally invasive surgeries for *** first-time report a patient with Kümmell disease and lumbar scoliosis treated with percutaneous kyphoplasty(PKP)under O-arm *** SUMMARY An 89-year-old female was admitted to the hospital due to delayed low back pain after a *** was diagnosed with Kümmell disease based on physical and radiologic *** patient experienced severe scoliosis and subsequently underwent O-arm-guided kyphoplasty,resulting in a significant alleviation of low back *** PKP has good efficacy in treating Kümmell ***,surgical risks are elevated in scoliosis patients with Kümmell disease due to the abnormal anatomical structure of the spine.O-arm assisted operations play a crucial role in decreasing surgical risks.

关键词： Kümmell disease Vertebral compression fracture Scoliosis Kyphoplasty Case report