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MicroRNA-142-3p and microRNA-142-5p are downregulated in hepatocellular carcinoma and exhibit synergistic effects on cell motility

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Frontiers of Medicine 2015年第3期9卷 331-343页

作者： Felice Ho-Ching Tsang Sandy leung-Kuen Au Lai Wei Dorothy ngo-Yin Fan Joyce Man-Fong Lee Carmen Chak-Lui Wong Irene Oi-Lin ng Chun-Ming Wong State Key Laboratory for Liver Research and Department of Pathology Li Ka Shing Faculty of Medicine The University of Hong Kong HongKong SAR China

MicroRNAs （miRNAs）, an important class of small non-coding RNAs, regulate gene expression at the post-transcriptional level, miRNAs are involved in a wide range of biological processes and implicated in different diseases, including cancers. In this study, miRNA profiling and qRT-PCR validation revealed that miR-142-3p and miR-142-5p were significantly downregulated in hepatocellular carcinoma （HCC） and their expression levels decreased as the disease progressed. The ectopic expression of miR-142 significantly reduced HCC cell migration and invasion. Overexpression of either miR-142-3p or miR-142-5p suppressed HCC cell migration, and overexpression of both synergistically inhibited cell migration, which indicated that miR-142-3p and miR-142-5p may cooperatively regulate cell movement, miR-142-3p and miR-142-5p, which are mature miRNAs derived from the 3＇- and 5＇-strands of the precursor miR-142, target distinct pools of genes because of their different seed sequences. Pathway enrichment analysis showed a strong association of the putative gene targets of miR-142-3p and miR-142-5p with several cell motility-associated pathways, including those regulating actin cytoskeleton, adherens junctions, and focal adhesion. Importantly, a number of the putative gene targets were also significantly upregulated in human HCC cells. Moreover, overexpression of miR-142 significantly abrogated stress fiber formation in HCC cells and led to cell shrinkage. This study shows that mature miR-142 pairs collaboratively regulate different components of distinct signaling cascades and therefore affects the motility of HCC cells.

关键词： hepatocellular carcinoma microRNA metastasis cytoskeletal reorganization

Attitudes of visitors at adult intensive care unit toward organ donation and organ support

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Chinese Medical Journal 2019年第3期132卷 373-376页

作者： nga-Wing Tsai Yee-Man leung Pauline Yeung ng Ting Liong Sui-Fong Lee Chun-Wai ngai Wai-Ching Sin Jenny Koo Wai-Ming Chan Adult Intensive Care Unit Queen Mary HospitalHong Kong 999077China Intensive Care Unit United Christian HospitalHong Kong 999077China Intensive Care Unit Kwong Wah HospitalHong Kong 999077China Central Nursing Department Queen Mary HospitalHong Kong 999077China

The concept of brain death was 1st introduced in the 1960s, providing an essential conceptual change to medical practice,[1] and its diagnosis is based on demonstrating the complete, irreversible cessation of brainste... 详细信息

关键词： brain death toward organ donation organ support

左心房应变与左心室射血分数轻度降低或保留的ST段抬高型心肌梗死患者血清N末端B型脑钠肽前体水平的相关性研究

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中国医药 2024年第6期19卷 815-819页

作者：吕赛梅迎晨韩蕊马旃王继红赵兴山雷海玲刘巍首都医科大学附属北京积水潭医院心内科北京大学第四临床医学院北京100035

目的探讨左心房应变与左心室射血分数(LVEF)轻度降低或保留的ST段抬高型心肌梗死(STEMI)患者血清N末端B型脑钠肽前体(NT-proBNP)水平的相关性。方法入选2022年3—12月于首都医科大学附属北京积水潭医院心内科首次诊断急性STEMI并在24 h... 详细信息

目的探讨左心房应变与左心室射血分数(LVEF)轻度降低或保留的ST段抬高型心肌梗死(STEMI)患者血清N末端B型脑钠肽前体(NT-proBNP)水平的相关性。方法入选2022年3—12月于首都医科大学附属北京积水潭医院心内科首次诊断急性STEMI并在24 h内行经皮冠状动脉介入(PCI)治疗,急性期LVEF>40%的患者。应用二维斑点追踪技术分析患者的左心房应变参数。根据左心房储存期纵向应变(LASr)数值将患者分为LASr减低组和LASr正常组。比较2组患者临床资料及超声资料。采用多元线性回归模型分析LASr与LVEF轻度降低或保留的STEMI患者血清NT-proBNP峰值的关系。结果51例接受PCI治疗且急性期LVEF>40%的急性STEMI患者中,LASr减低组22例(43.1%),LASr正常组29例(56.9%)。LASr减低组年龄、NT-proBNP峰值均高于LASr正常组[(60±12)岁比(50±12)岁、1679.0(811.4,2133.2)ng/L比584.2(201.5,1524.0)ng/L],差异均有统计学意义(均P<0.05)。LASr减低组左心房通道期纵向应变、左心房收缩期应变高于LASr正常组,差异均有统计学意义(均P<0.05)。多元线性回归模型分析结果表明,校正前壁心肌梗死、肌酸激酶同工酶峰值、LVEF后,LASr是LVEF轻度降低或保留的STEMI患者血清NT-proBNP峰值的独立影响因素(P=0.039)。结论LASr是LVEF轻度降低或保留的STEMI患者PCI治疗围手术期NT-proBNP峰值的独立影响因素。

关键词：急性ST段抬高型心肌梗死经皮冠状动脉介入左心房应变 N末端B型脑钠肽前体

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香港人工斜坡与生态工法设计之探讨

水土保持学报 2003年第3期17卷 82-85页

作者：伍世良香港中文大学地理与资源管理学系

香港地少人多,市区人口密度之高,为世界之冠,超过5300人/km2。为制造出更多的平地空间,改造斜坡便成为香港市区发展的重要因素之一。早期人工斜坡的设计,多以结构体之安全性和稳定性为考量。随着市民环境保护意识之提高,现今的斜坡设计... 详细信息

香港地少人多,市区人口密度之高,为世界之冠,超过5300人/km2。为制造出更多的平地空间,改造斜坡便成为香港市区发展的重要因素之一。早期人工斜坡的设计,多以结构体之安全性和稳定性为考量。随着市民环境保护意识之提高,现今的斜坡设计,除了须满足一般之城市规划要求外,更需考虑环境之美化和生态之效益。本文介绍生态工法设计在香港斜坡工程上的各种应用,以探讨其优劣处、局限性、可行性和综合效益。

关键词：香港人工斜坡生态工法设计应用斜坡工程水土保持环境美化

Hypoxia and Escherichia coli LPS-induced periodontal resident cells PAMP expressions

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Hypoxia and Escherichia coli LPS-induced periodontal residen...

分支定价方法求解带二维装箱约束的车辆路径问题

第九届全国免疫学学术大会

作者： Chen Yu sai Wah Tsao W.Keung leung Faculty of Dentistry LKS Faculty of MedicineThe University of Hong Kong Department of Anatomy LKS Faculty of MedicineThe University of Hong Kong

Background:The recognition of anaerobic periodontopathogens related molecular patterns(PAMP)by Toll-like(TL)and/or NOD-like receptors(NLRs)of human gum lining cells could lead to local chronic inflammation and eventua...

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控制理论与应用 2023年第3期40卷 409-418页

作者：季彬周赛琦张政中南大学交通运输工程学院湖南长沙410075

面向家具、电器等货物的物流配送场景,研究带二维装箱约束的车辆路径问题(2L–CVRP),构建了2L–CVRP的混合整数线性规划模型.为求解大规模2L–CVRP,构建了该问题集合划分模型,提出基于分支定价的方法.针对分支节点的松弛模型,基于列生... 详细信息

面向家具、电器等货物的物流配送场景,研究带二维装箱约束的车辆路径问题(2L–CVRP),构建了2L–CVRP的混合整数线性规划模型.为求解大规模2L–CVRP,构建了该问题集合划分模型,提出基于分支定价的方法.针对分支节点的松弛模型,基于列生成策略将其分解为线性规划主问题、带资源和二维装箱约束的最短路径子问题,并提出基于ng-route松弛策略的标签算法和基于禁忌搜索的装箱算法有效求解复杂子问题.仿真结果表明,提出的方法可高效求解大规模2L–CVRP,其中ng-route松弛策略能有效提升算法求解效率,研究成果为装箱约束下大规模车辆路径问题的高效求解提供了有效途径.

关键词：车辆路径混合整数线性规划分支定价二维装箱问题

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Extensive contact tracing and screening to control the spread of vancomycin-resistant Enterococcus faecium ST414 in Hong Kong

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Chinese Medical Journal 2012年第19期125卷 3450-3457页

作者： CHEng Vincent Chi-chung TAI Josepha Wai-ming ng Modissa Lai-ming CHAN Jasper Fuk-woo WOng Sally Cheuk-ying LI Iris Wai-sum CHUng Hon-ping LO Wai-kei YUEN Kwok-yung HO Pak-leung Department of Microbiology Queen Mary Hospital Hong Kong Special Administrative Region China Infection Control Team Queen Mary Hospital Hong Kong Special Administrative Region China Department of Surgery Tung Wah Hospital Hong Kong Special Administrative Region China Infection Control Team Tung Wah Hospital Hong Kong Special Administrative Region China

Background Proactive infection control management is crucial in preventing the introduction of multiple drug resistant organisms in the healthcare setting. In Hong Kong, where vancomycin-resistant enterococci （VRE） endemicity is not yet established, contact tracing and screening, together with other infection control measures are essential in limiting intra- and inter-hospital transmission. The objective of this study was to illustrate the control measures used to eradicate a VRE outbreak in a hospital network in Hong Kong. Methods We described an outbreak of VRE in a healthcare region in Hong Kong, involving a University affiliated hospital and a convalescent hospital of 1600 and 550 beds respectively. Computer-assisted analysis was utilized to facilitate contact tracing, followed by VRE screening using chromogenic agar. Multi-locus sequence typing （MLST） was performed to assess the clonality of the VRE strains isolated. A case-control study was conducted to identify the risk factors for nosocomial acquisition of VRE. Results Between November 26 and December 17, 2011, 11 patients （1 exogenous case and 10 secondary cases） in two hospitals with VRE colonization were detected during our outbreak investigation and screening for 361 contact patients, resulting in a clinical attack rate of 2.8% （10/361）. There were 8 males and 3 females with a median age of 78 years （range, 40-87 years）. MLST confirmed sequence type ST414 in all isolates. Case-control analysis demonstrated that VRE positive cases had a significantly longer cumulative length of stay （P 〈0.001）, a higher proportion with chronic cerebral and cardiopulmonary conditions （P=0.001）, underlying malignancies （P 〈0.001）, and presence of urinary catheter （P 〈0.001）, wound or ulcer （P 〈0.001）, and a greater proportion of these patients were receiving β-lactam/ β-1actamase inhibitors （P=0.009）, carbapenem group （P 〈0.001）, fluoroquinolones （P=0.003）, or vancomycin （P=0.001） when compared with the controls. Conclusion

关键词： vancomycin-resistant Enterococcus faecium ST414 outbreak contact tracing screening

An Improved Differential Evolution and Its Industrial Application

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Journal of Intelligent Learning Systems and Applications 2012年第2期4卷 81-97页

作者： Johnny Chung Yee Lai Frank Hung Fat leung sai Ho Ling Edwin Chao Shi Centre for Health Technologies Faculty of Engineering and IT University of Technology Sydney Sydney Australia. Centre for Signal Processing Department of Electronic and Information Engineering The Hong Kong Polytechnic University Hung Hom Hong Kong China

In this paper, an improved Differential Evolution (DE) that incorporates double wavelet-based operations is proposed to solve the Economic Load Dispatch (ELD) problem. The double wavelet mutations are applied in order to enhance DE in exploring the solution space more effectively for better solution quality and stability. The first stage of wavelet operation is embedded in the DE mutation operation, in which the scaling factor is governed by a wavelet function. In the second stage, a wavelet-based mutation operation is embedded in the DE crossover operation. The trial population vectors are modified by the wavelet function. A suite of benchmark test functions is employed to evaluate the performance of the proposed DE in different problems. The result shows empirically that the proposed method out-performs signifycantly the conventional methods in terms of convergence speed, solution quality and solution stability. Then the proposed method is applied to the Economic Load Dispatch with Valve-Point Loading (ELD-VPL) problem, which is a process to share the power demand among the online generators in a power system for minimum fuel cost. Two different conditions of the ELD problem have been tested in this paper. It is observed that the proposed method gives satisfactory optimal costs when compared with the other techniques in the literature.

关键词： Differential Evolution Evolutionary Algorithm Economic Load Dispatch

The RNA Pol Ⅱ sub-complex hsRpb4/7 is required for viability of multiple human cell lines

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Protein & Cell 2012年第11期3卷 846-854页

作者： Yang Zhao Kim K.C.Li King Pan ng Chi Ho ng Kevin A.W.Lee Division of Life Science The Hong Kong University of Science and TechnologySai KungHong Kong S.A.R.China

The evolutionarily conserved RNA Polymerase Ⅱ Rpb4/7 sub-complex has been thoroughly studied in yeast and impacts gene expression at multiple levels including transcription,mRNA processing and *** addition Rpb4/7 exerts differential effects on gene expression in yeast and Rpb4 is not obligatory for yeast(***)*** roles for human(hs)Rpb4/7 have not been extensively described and we have probed this question by depleting hsRpb4/7 in established human cell lines using RNA *** find that Rpb4/7 protein levels are inter-dependent and accordingly,the functional effects of depleting either protein are ***4/7 exhibits gene-specific effects and cells initially remain viable upon hsRpb4/7 *** prolonged hsRpb4/7 depletion is cytotoxic in the range of cell lines *** cell death occurs by an unknown mechanism and in some cases is accompanied by a pronounced elongated cell *** conclusion we provide evidence for a gene-specific role of hsRpb4/7 in human cell viability.

关键词： RNA PolymeraseⅡ hsRpb4/7 sub-complex gene expression cell viability cell shape