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A chromatographic method for pursuing potential GPCR ligands with the capacity to characterize their intrinsic activities of regulating downstream signaling pathway

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Chinese Chemical Letters 2024年第10期35卷 425-429页

作者： Ting Li Xinxin Zheng Lejing Qu Yuanyuan Ou sai Qiao Xue Zhao Yajun Zhang Xinfeng Zhao Qian Li Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of EducationCollege of Life SciencesNorthwest UniversityXi'an 710069China

GPCRs are dominant targets for approved drugs and the discovery of lead compound targeting them is still ***-based screening technique is a promising platform to uncover GPCR ***,the intrinsic activities of them are seldom simultaneously determined during the *** beta2-adrenoceptor(β2AR)as a probe,this work created a strategy for screening GPCR ligands with simultaneous characterization of their downstream G protein binding responses associated with *** strategy included(i)the design and expression of a protein miniature formed byβ2AR and G proteinα-subunit(Gαs)using circularly permuted HaloTag(cpHalo)as a flexible linker;(ii)immobilization of the miniature onto silica gel by a click dehalogenation reaction;(iii)systematic characterization of the immobilized miniature by fluorescent and chromatographic studies,and(iv)simulating of ligand-inducedβ2AR-Gαs signaling cascade by chromatographic assays using GTP as an *** immobilized miniature exhibited specificity toβ2AR and Gαs antibodies and *** specificity is stable at least within fifteen days with the variation less than 1%.The intrinsic activities ofβ2AR ligands were distinguished by the changes of GTP chromatographic behaviors on Gαs-cpHalo-β2AR *** strengthened the binding affinity and kinetics of GTP with Gαs,while antagonist did not give any effect on *** the intrinsic activity evaluation,we believe,it will improve the attributes of chromatographic methods for drug discovery efforts with minimizing false-positive results.

关键词： Intrinsic efficacy GPCR-G protein miniature Circularly permuted HaloTag High-performance receptor chromatography GTP binding Agonist/antagonist

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Suppression of host plant defense by bacterial small RNAs packaged in outer membrane vesicles

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Plant Communications 2024年第4期5卷 252-254页

作者： Yan Wu sai Wang Peihong Wang Wenhan Nie Iftikhar Ahmad Panagiotis F.Sarris Gongyou Chen Bo Zhu Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station Shanghai Cooperative Innovation Center for Modern Seed Industry School of Agriculture and Biology Shanghai Jiao Tong University Shanghai China Department of Environmental Sciences COMSATS University Islamabad Vehari-Campus Vehari 61100 Pakistan Department of Biology University of Crete 714 09 Heraklion Crete Greece Shanghai Jiao Tong University Chongqing Research Institute Shanghai China

(Plant Communications 5,100817;April 82024)After a careful,in-depth examination,the authors found some additional errors in the published *** revision,the authors created a mutant DXosr001(in which the full-length Xosr001 was deleted)and a mutant DXosr001’(in which the predicted binding motif of Xosr001 was deleted)as shown in Figure S4 of the original *** RNA-FISH assays,Xosr001 transcript could not be detected in leaves inoculated with DXosr001-OMVs,while leaves inoculated with WT-and DXosr001’-OMVs could emit green fluorescence.

关键词： deleted Figure outer

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Development and application of photocatalytic coating for roadside NO_(x) mitigation in Hong Kong

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Chinese Chemical Letters 2024年第3期35卷 153-157页

作者： Xinwei Li Pengge Wang Shuwen Han Yu Huang Wingkei Ho Steven sai Hang Ho Shun-cheng Lee Meng Wang Department of Civil and Environmental Engineering The Hong Kong Polytechnic UniversityHong KongChina State Key Laboratory of Loess and Quaternary Geology(SKLLQG)and Key Laboratory of Aerosol Chemistry and Physics Institute of Earth EnvironmentChinese Academy of SciencesXi'an 710061China Department of Science and Environmental Studies The Education University of Hong KongHong KongChina Division of Atmospheric Sciences Desert Research InstituteRenoNV 89512United States

A facile chemical method for the development of photocatalytic coating products was proposed based on practical application perspective for the Hong Kong roadside nitrogen oxides(NO_(x))***_(2)-based photocatalytic coating PC-C film with crystallized size of around 5–6 nm was synthesized with the peptization of H_(2)O_(2).The PC-C coating possesses a super-hydrophilicity surface and is proven to have a NO_(x)degradation rate of 46.8%with an optimum pH level of *** addition,the PC-C coating presents a promising photocatalytic NO_(x)degradation compared with other commercially available coating products and P25 when applied on two building materials of poly-methyl methacrylate(PMMA)and concrete surface.A weather resistance simulation and a 180-day on-site field trial were carried out the attenuation effects of photocatalytic coating applied in outdoor *** on epidemiological estimation and field investigation,hospital admissions for respiratory diseases(HARD)and mortality cases(MC)could be reduced with the application of PC-C coating along the street *** work demonstrates the feasibility of air pollution control measures for the local roadside NO_(x)using photocatalytic technology,offering promising health benefits with environmental remediation.

关键词： Photocatalysis Coating NO_(x) mitigation Hong Kong Roadside

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Describing Hadsorption on dual-active sites of single-atom-metals and frustrated-Lewis-pairs for reverse water-gas shift reaction

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Science China Chemistry 2024年

作者： Wenbin Li Jie Gan Qing Guo sai Zhang Yongquan Qu School of Chemistry and Chemical Engineering Northwestern Polytechnical University School of Materials and Environmental Engineering Chizhou University Research & Development Institute of Northwestern Polytechnical University in Shenzhen

Acquiring descriptors to guide catalyst design is an ideal but challenging topic in the field of heterogeneous catalysis. Herein, the adsorption energy of H*(Ead（H）) has been established to describe the catalytic activity of dual-active sites of single-atom metals and frustrated Lewis pairs （FLPs） on the porous nanorod of CeO2surface （M1/PN-CeO2） for reverse water-gas shift （RWGS）reaction. H2and CO2molecules are adsorbed and activated on single-atom metals and FLP sites, respectively, facilitating the RWGS reaction through the H*spillover from single-atom metals to PN-CeO2supports. Mechanistic investigations illustrate that the generation of H*on the single-atom metals and subsequent migration towards FLP are critical steps, determining the performance of M1/PN–CeO2for RWGS reaction. A volcanic correlation is observed between Ead（H） and catalytic activity of M1/PN–CeO2for RWGS. The Pt1/PN–CeO2catalysts with a minimal deviation of-0.01 eV from the experimentally optimized Ead（H） of-1.88 eV, exhibited an exceptional turnover frequency of 3.,605 h-1and surpassed a turnover number of 20,000,000for each Pt active site during a period of 650 h at 600°C. These findings could pave the way for the rational design of highly active and durable catalysts for RWGS reaction.

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重组生物技术制品生产工艺变更的病毒安全性风险再评估策略

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Engineering 2024年第1期32卷 14-17页

作者： Wenbo sai Dongchen Jia Hao Chen Wei Wei Center for Drug Evaluation National Medical Products AdministrationBeijing 100076China

In the manufacturing of biotechnology drug products,the viral safety control strategy is a critical pharmaceutical quality management framework that contains three key elements:prevention,testing,and *** prevention strategy involves rigorous screening for any adventitious virus contamination in the raw materials,reagents,and endogenous/adventitious virus contamination in the cell banks.

关键词： prevention testing remove

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Evolution and function of residual solvent in polymer-Li_2B_(12)H_(12) composite solid electrolyte

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Rare Metals 2024年第8期43卷 3748-3757页

作者： Xiang-Yang Ye Ke-Pan Bao sai-Nan Luo Xin Li Tai-Qiang Chen Shui-Xin Xia Tao Yuan Yue-Peng Pang Shi-You Zheng School of Materials and Chemistry University of Shanghai for Science and TechnologyShanghai 200093China Department of Materials Science and Engineering National University of SingaporeSingapore 119077Singapore

Composite solid electrolytes(CSEs) containing polymer matrices and inorganic fillers hold promise for the next generation of solid-state ***,the role of residual solvents in CSEs remains *** study investigated the evolution and function of the residual solvent in a polymer-Li_2B_(12)H_(12) CSE.A partial reaction occurred between Li_2B_(12)H_(12) and solvent N,N-dimethylformamide(DMF),which produced dimethylaminomethanol(DMAM) in the *** functional theory calculations have revealed that DMA M forms stronger hydrogen bonds with polyvinylidene fluoride chains than DMF,which can have a plasticizing effect on the polymer matrix,leading to lower crystallinity and quicker segment ***,this CSE exhibited improved Li-ion conducting properties,enabling the stable cycling of Li‖LiFePO_(4) solid-state *** study provided insights into the role of residual solvents in CSEs.

关键词： Composite solid electrolyte Residual solvent Lithium borohydride Plasticizing effect

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Elaborations of the influencing factors on the formation of secondary inorganic aerosols in a heavily polluted urban area of China

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Journal of Environmental Sciences 2024年第4期138卷 406-417页

作者： Shuang Wang Qiyuan Wang Ting Zhang Suixin Liu Steven sai Hang Ho Jie Tian Hui Su Yong Zhang Luyao Wang Tingting Wu Junji Cao School of Human Settlements and Civil Engineering Xi’an Jiaotong UniversityXi’an 710049China Key Laboratory of Aerosol Chemistry and Physics State Key Laboratory of Loess and Quaternary GeologyInstitute of Earth EnvironmentChinese Academy of SciencesXi’an 710061China CAS Center for Excellence in Quaternary Science and Global Change Xi’an 710061China National Observation and Research Station of Regional Ecological Environment Change and Comprehensive Management in the Guanzhong Plain Xi’an 710061China Division of Atmospheric Sciences Desert Research InstituteRenoNevada89512United States Hong Kong Premium Services and Research Laboratory KowloonHong Kong SARChina Xi’an Institute for Innovative Earth Environment Research Xi’an 710061China Institute of Atmospheric Physics Chinese Academy of SciencesBeijing 100029China

In this study,online water-soluble inorganic ions were detected to deduce the formation mechanism of secondary inorganic aerosol in Xianyang,China during *** dominant inorganic ions of sulfate(SO_(4)^(2-)),nitrate(NO_(3.^(-)),and ammonium(NH_(4)^(+))(the sum of those is abbreviated as SNA)accounted for 17%,21%,and 12% of PM_(2.5)mass,*** the air quality deteriorated from excellent to poor grades,the precursor gas sulfur dioxide(SO_(2))of SO_(4)^(2-)increased and then decreased with a fluctuation,while nitrogen dioxide(NO_(2))and ammonia(NH_(3.),precursors of NO_(3.^(-)and NH_(4)^(+),and SNA show increasing *** factors including boundary layer height(BLH),temperature,and wind speed also show decline trends,except relative humidity(RH).Meanwhile,the secondary conversion ratio shows a remarkable increasing trend,indicating that there was a strong secondary *** the perspective of chemical mechanisms,RH is positively correlated with sulfur oxidation ratios(SOR),nitrogen oxidation ratios(NOR),and ammonia conversion ratios,representing that the increase of humidity could promote the generation of ***,SOR and NOR were also positively related to the *** the one hand,the low wind speed and BLH led to the accumulation of *** the other hand,the increases of RH and ammonia promoted more formations of SNA and PM_(2.5).The results advance our identification of the contributors to the haze episodes and assist to establish more efficient emission controls in Xianyang,in addition to other cities with similar emission and geographical characteristics.

关键词： Haze episodes Secondary inorganic aerosol Secondary conversion ratio Meteorological factors

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基于冻融循环本构的预应力对拉式挡墙的冻胀效应研究

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中南大学学报（自然科学版） 2024年第1期55卷 242-254页

作者：庄妍王赛东南大学土木工程学院江苏南京210000

预应力对拉式挡墙的冻胀受力状态复杂,墙背的冻胀力分布尚不明确,进一步探究预应力对拉式挡墙的冻胀力分布具有重要意义。根据哈尔滨某地区的实际监测温度,采用ABAQUS数值模拟软件模拟温度场在模型空间的合理分布。基于冻融循环后混凝... 详细信息

预应力对拉式挡墙的冻胀受力状态复杂,墙背的冻胀力分布尚不明确,进一步探究预应力对拉式挡墙的冻胀力分布具有重要意义。根据哈尔滨某地区的实际监测温度,采用ABAQUS数值模拟软件模拟温度场在模型空间的合理分布。基于冻融循环后混凝土塑性损伤本构,在所建立的温度场上进行热力耦合分析,研究预应力筋、冻胀时间对预应力对拉式挡墙冻胀效应的影响。研究结果表明:挡土墙所受冻胀力呈现“上小下大”的上三角分布趋势,且随着冻胀时间增加,冻胀力会呈现先逐渐增大然后逐渐减小的趋势,并在冻胀后120 d冻胀力达到最大。相比之下,由于预应力钢筋的存在,预应力对拉式挡土墙会使冻胀力在该处产生应力集中而增大,最大可使冻胀力增加200 kPa左右。在预应力钢筋的作用下,挡土墙的水平位移会减小40%~116%。本文研究结果可以为季节性冻土区的预应力对拉式挡土墙施工和设计提供技术支撑。

关键词：数值模拟预应力对拉式挡土墙冻胀效应季节性冻土

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Aggregation-Induced Catalysis: Asymmetric Catalysis with Chiral Aggregates

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Research 2024年第1期2023卷 525-532页

作者： Yao Tang Yu Wang Qingkai Yuan sai Zhang Jia-Yin Wang Shengzhou Jin Ting Xu Junyi Pan Kazimierz Surowiec Guigen Li Department of Chemistry and Biochemistry Texas Tech UniversityLubbockTX 79409-1061USA School of Chemistry and Chemical Engineering Nanjing UniversityNanjing 210093China School of Pharmacy Continuous Flow Engineering Laboratory of National Petroleum and Chemical IndustryChangzhou UniversityChangzhouJiangsu 213164China

So far,there have been 4 methods to control chirality including the use of chiral auxiliaries,reagents,solvents,and catalysts documented in literature and *** them,asymmetric catalysts are normally divided into homogeneous and heterogeneous *** this report,we present a new type of asymmetric control—asymmetric catalysis via chiral aggregates that would not belong to the above *** new strategy is represented by catalytic asymmetric dihydroxylation reaction of olefins in which chiral ligands are aggregated by taking advantage of typical aggregation-induced emission systems containing tetrahydrofuran and H_(2)O *** was proven that the chiral induction can be enhanced from er of 78:22 to 97:3.simply by changing the ratios of these 2 cosolvents.

关键词： chiral asymmetric Chiral

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Community-acquired pneumonia:The importance of the early detection of drug-resistant organisms

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World Journal of Critical Care Medicine 2024年第2期13卷 11-14页

作者： sai Doppalapudi Muhammad Adrish Department of Medicine Bronx Care Health SystemNew York Affiliated with The Icahn School of Medicine at Mount SinaiBronxNY 10457United States Section of Pulmonary Critical Careand Sleep MedicineBaylor College of MedicineHoustonTX 77030United States

Pneumonia is a disease associated with significant healthcare burden with over 1.5 million hospitalizations annually and is the eighth leading cause of death in the United *** community-acquired pneumonia(CAP)is generally considered an acute time-limited illness,it is associated with high long-term mortality,with nearly one-third of patients requiring hospitalization dying within one *** increasing trend of detecting multidrug-resistant(MDR)organisms causing CAP has been observed,especially in the Western *** this editorial,we discuss about a publication by Jatteppanavar et al which reported that a case of a MDR organism was the culprit in developing pneumonia,bacteremia,and infective endocarditis that led to the patient’s *** early detection of these resistant organisms helps improve patient *** advances have been made in the biotechnological and research space,but preventive measures,diagnostic techniques,and treatment strategies need to be developed.

关键词： Methicillin-resistant Staphylococcus aureus Polymerase chain reaction Antibiotic resistance Bacterial colonization Infective endocarditis

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