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In Vitro Antioxidant and Radio Protective Activities of Lycopene from Tomato Extract against Radiation—Induced DNA Aberration

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Journal of Biosciences and Medicines 2024年第2期12卷 202-213页

作者： safaiatul islam Abu Hena Mostofa Kamal Md. Ziaur Rahman Protul Kumar Roy A.Y.K. Md. Masud Rana Molecular Radiobiology and Biodosimetry Division Institute of Food and Radiation Biology Bangladesh Atomic Energy Commission Savar Bangladesh

Background: The accumulation of free radicals is linked to a number of diseases. Free radicals can be scavenged by antioxidants and reduce their harmful effects. It is therefore essential to look for naturally occurring antioxidants that come from plants, as synthetic antioxidants are toxic, carcinogenic and problematic for the environment. Lycopene is one of the carotenoids, a pigment that dissolves in fat and has antioxidant properties. Materials and Methods: The antioxidant and free radical scavenging activity were assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The impact of lycopene on bacteria (E. coli) susceptibility to γ-radiation was examined by radio sensitivity assay. The study also examined the induction of strand breaks in plasmid pUC19 DNA and how lycopene extract protected the DNA from γ-radiation in vitro. Results: At varying concentrations, lycopene demonstrated its ability to scavenge free radicals such as 2, 2-diphenyl-1-picrylhydrazyl (DPPH). IC50 for lycopene was determined at 112 μg/mL which was almost partial to IC50 of standard antioxidant L-ascorbic acid. The D10 value 180 Gy of E. coli was found to be >2-fold higher in the extract-containing lycopene sample than in the extract-free controls. The lycopene extracts inhibited the radiation-induced deterioration of the plasmid pUC19 DNA. At an IC50 concentration, lycopene provided the highest level of protection. Conclusion: Lycopene functions as an efficient free radical scavenger and possible natural antioxidant source. For cancer patients and others who frequently expose themselves to radiation, lycopene may be a useful plant-based pharmaceutical product for treating a variety of diseases caused by free radicals.

关键词： Radio Protective Antioxidants Free Radical DNA Damage pUC19 Plasmid Gamma Irradiation DPPH

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Assessment of Physicochemical and Bacteriological Quality of Different Surface Water Samples of Tangail District, Bangladesh

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Advances in Microbiology 2017年第11期7卷 769-789页

作者： Nasreen Jahan safaiatul islam Fatematuz Zuhura Evamoni Md. Jakir Hossain Sabiha Akter Md. Ronzu Ahmmed Md. Ashraful islam Shahin Mahmud Department of Biotechnology and Genetic Engineering Mawlana Bhashani Science and Technology University Santosh Tangail Department of Biotechnology and Genetic Engineering Noakhali Science and Technology University Sonapur Noakhali Bangladesh Department of Environmental Science and Resource Management Mawlana Bhashani Science and Technology University Santosh Tangail Department of Statistics Mawlana Bhashani Science and Technology University Santosh Tangail

Surface water, a vital element of ecosystem must be hygienic, but unfortunately the pollution of this water is now an alarming problem. The present work deals with the assessment of physicochemical and bacteriological profile of several untreated surface water sources to ensure its suitability for using domestic purposes and drinking along with the impact of bacterial contaminated water on public health and antibiotic resistance pattern of these bacteria. The samples were collected from twenty sampling point (13 ponds, 4 lakes, 2 rivers and one canal) of Tangail District from January 2017 to July 2017. To understand the seasonal variation, water samples were measured in two month;February (dry season) and June (wet season). Most of the water sampling points were polluted by dumping of waste, cattle wash and were not suitable for the drinking or other domestic purposes. Obtained results showed that water of the study area is slightly alkaline, no remarkable variation in the temperature of the water in both seasons and DO of all the sampling station were lower than standard limit during February. The mean concentration (M.C.) of total Coliform were 4 × 1022 cfu/100ml and 4.5 × 1018 cfu/100ml at February and June respectively and all the sampling point were contaminated with fecal coliform. Other pathogenic bacteria, E. coli (M.C: 1.1 × 1011 cfu/100ml at February and M.C: 2.2 × 107 cfu/100ml at June), Salmonella spp. (M.C: 1.1 × 105 cfu/100ml at February and M.C: 3.4 × 105 cfu/100ml at June), Shigella spp. (M.C: 8 × 104 cfu/100ml at February and M.C: 3.4 × 107 cfu/100ml at June), Vibrio spp. (M.C: 8.6 × 105 cfu/100ml at February and M.C: 1.1 × 108 cfu/100ml at June) were isolated from several of the investigated water sources. The total counts of these pathogenic bacteria exceeded the acceptable limit during both season and also showed resistance against a broad range of commercially available antibiotics. People who were using these water frequently suffering from var

关键词： pH Dissolved Oxygen Coliform E. coli Shigella spp. Salmonella spp. Vibrio spp. Antibiotic Tangail

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