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Rapid detection of coliforms in drinking water of Arak city using multiplex PCR method in comparison with the standard method of culture(Most probably Number)

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Asian Pacific Journal of Tropical Biomedicine 2014年第5期4卷 404-409页

作者： Dehghan fatemeh Zolfaghari Mohammad Reza Arjomandzadegan Mohammad Kalantari Salomeh Ahmari Gholam Reza Sarmadian Hossein sadrnia maryam Ahmadi Azam Shojapoor Mana Najarian Negin Kasravi Alii Reza Falahat Saeed Department of Microbiology Qom BranchIslamic Azad University Tuberculosis and Infectious Research Center and Department of Microbiology Arak University of Medical Sciences Hygiene and Quality Control Office of Markazi Province Water and Wastewater Company Department of Biology Payame Noor University Molecular and Medicine Research Center Arak University of Medical Sciences Department of Microbiology Arak University of Medical Sciences Department of Microbiology Islamic Azad UniversitySciences and Research Branch

Objective:To analyse molecular detection of coliforms and shorten the time of ***:Rapid detection of coliforms by amplification of lacZ and uidA genes in a multiplex PCR reaction was designed and performed in comparison with most probably number(MPN)method for 16 artificial and 101 field *** molecular method was also conducted on isolated coliforms from positive MPN samples;standard sample for verification of microbial method certificated reference material;isolated strains from certificated reference material and standard *** PCR and electrophoresis parameters were changed for reducing the operation ***:Results of PCR for lacZ and uidA genes were similar in all of standard,operational and artificial samples and showed the 876 bp and 147 bp bands of lacZ and uidA genes by multiplex *** results were confirmed by MPN culture method by sensitivity 86%(95%CI:0.71-0.93).Also the total execution time,with a successful change of factors,was reduced to less than two and a half ***:Multiplex PCR method with shortened operation time was used for the simultaneous detection of total coliforms and Escherichia coli in distribution system of Arak ***'s recommended to be used at least as an initial screening test,and then the positive samples could be randomly tested by MPN.

关键词： Water Coliforms PCR Rapid detection

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Smart Anti-Pinch Window Simulation Using H_/H_(∞)Criterion and MOPSO

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Computers, Materials & Continua 2022年第7期72卷 215-226页

作者： Maedeh Mohammadi Azni Mohammad Ali sadrnia Shahab S.Band Zulkefli Bin Mansor Department of Electrical and Robotics Engineering Shahrood University of TechnologySemnanIran Future Technology Research Center College of FutureNational Yunlin University of Science and Technology 123 University RoadSection 3DouliouYunlin64002Taiwan Faculty of Information Science and Technology Universiti Kebangsan Malaysia43600UKM BangiSelangorMalaysia

Automobile power windows are mechanisms that can be opened and shut with the press of a *** these windows can comfort the effort of occupancy to move the window,failure to recognize the person’s body part at the right time will result in damage and in some cases,loss of that *** anti-pinch mechanism is an excellent choice to solve this problem,which detects the obstacle in the glass path immediately and moves it *** this paper,an optimal solution H_/H_(∞)is presented for fault detection of the anti-pinch window *** anti-pinch makes it possible to detect an obstacle and prevent damages through sampling parameters such as current consumption,the speed and the position of DC *** this research,a speed-based method is used to detect the *** order to secure the anti-pinch window,an optimal algorithm based on a fault detection observer is *** the residual design,the proposed fault detection algorithm uses theDCmotor angular velocity *** against disturbances and sensitivity to the faults are considered as an optimization problem based on Multi-Objective Particle Swarm Optimization ***,an optimal filter for solving the fault problem is designed using the H_/H_(∞)*** results show that the simulated anti-pinch window is pretty sensitive to the fault,in the sense that it can detect the obstacle in 50 ms after the fault occurrence.

关键词： H_/H_(∞) anti-pinch residual fault detection multi-objective particle swarm optimization(MOPSO) multi-objective optimization automotive power windows electric windows

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