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Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells

Journal of Molecular Cell Biology 2011年第2期3卷 91-98页

作者： Zhao Cheng sachiko ito Naomi Nishio Hengyi Xiao Rong Zhang Haruhiko Suzuki Yayoi Okawa Toyoaki Murohara Ken-ichi Isobe Department of Immunology Nagoya University Graduate School of Medicine65 Turumai-choShowa-kuNagoyaAichi 466-8520Japan Laboratory for Geriatrics State Key Laboratory of BiotherapySichuan UniversityChengdu 610041China Department of Functioning Activation National Institute for Longevity SciencesNational Center for Geriatrics GerontologyAichi 463-0003Japan Department of Cardiology Nagoya University Graduate School of Medicine65 Turumai-choShowa-kuNagoyaAichi 466-8520Japan

If induced pluripotent stem(iPS)cells are to be used to treat damaged tissues or repair organs in elderly patients,it will be necessary to establish iPS cells from their *** determine the feasibility of using this technology with elderly patients,we asked if it was indeed possible to establish iPS cells from the bone marrow(BM)of aged *** cells from aged C57BL/6 mice carrying the green fluorescence protein(GFP)gene were cultured with granulocyte macrophage-colony stimulating factor(GM-CSF)for 4 *** factors(Oct3/4,Sox2,Klf4 and c-Myc)were introduced into the BM-derived myeloid(BM-M)*** efficiency of generating iPS cells from aged BM cultured in GM-CSF was ***,we succeeded in obtaining BM-M-iPS cells from aged C57BL/6 mice,which carried *** BM-M-iPS cells expressed SSEA-1 and Pou5f1 and were positive for alkaline phosphatase *** iPS cells did make teratoma with three germ layers following injection into syngeneic C57BL/6 mice,and can be differentiated to three germ layers in *** co-culturing with OP9,the BM-M-iPS cells can be differentiated to the myeloid *** differentiated BM-M-iPS cells proliferated well in the presence of GM-CSF,and lost expression of Nanog and Pou5f1,at least in part,due to methylation of their *** the contrary,Tnf and Il1b gene expression was upregulated and their promoters were hypomethylated.

关键词： induced pluripotent stem cells aged mouse bone marrow myeloid cells gene expression methylation ChIP assay

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Roles of N-cadherin in cerebral cortical development:cooperation with membrane trafficking and actin cytoskeletal regulation

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Neural Regeneration Research 2025年第1期20卷 188-190页

作者： Shiho ito Takeshi Kawauchi Department of Adaptive and Maladaptive Responses in Health and Disease Graduate School of MedicineKyoto UniversityKyotoJapan Department of Physiology Keio University School of MedicineTokyoJapan

Cell adhesion plays pivotal roles in the morphogenesis of multicellular *** cells form several types of cell-to-cell adhesion,including zonula occludens(tight junctions),zonula adhaerens(adherens junctions),and macula adhaerens(desmosomes).Although these adhesion complexes are basically observed only in epithelial cells,cadherins,which are the major cell adhesion molecules of adherens junctions,are expressed in both epithelial and non-epithelial tissues,including neural tissues(Kawauchi,2012).The cadherin superfamily consists of more than 100 members,but classic cadherins.

关键词： cerebral skeletal cooperation

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Research in Astronomy and Astrophysics 2021年第5期21卷 43-70页

作者： Yuta ito Department of Physics The Graduate Center of CUNY365 Fifth AvenueNew YorkNY 10016USA Department of Physics and Astronomy The College of Staten Island of CUNY2800 Victory BoulevardStaten IslandNY 10314USA

Fitting parametric models to globular clusters’structural profiles has been essential for the study of stellar *** provides their important structural parameters,such as the concentrations and core radii of the ***,existing parametric models can apply only to non-collapsing-core clusters in the early relaxation-evolution ***,a single parametric model cannot provide globular clusters’structural parameters in both the early and late evolution *** have recently found an accurate spectral solution for the self-similar orbit-averaged Fokker-Planck(OAFP)equation to model collapsingcore clusters at the late evolution *** present work establishes a new parametric model by combining the self-similar OAFP-and *** it is a single-mass and isotropic model,the new model applies to at least fifty-five Galactic globular clusters with resolved cores in all the evolution *** a main result,we show the characteristics of the relaxation times against the concentrations of the *** also affirm that the structures of low-concentration clusters are polytropic in the Milky Way.

关键词： (Galaxy:)globular clusters:general Galaxy:kinematics and dynamics methods:numerical

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Diethyldithiocarbamate inhibits iNOS expression in human lens epithelial cells stimulated by IFN-gamma and LPS

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Acta Pharmacologica Sinica 2005年第3期26卷 359-363页

作者： Yoshimasa ito Japan Osaka 577-8502 School of Pharmacy Pharmaceutical Research und Technology Institute Kinki University

Aim:To investigate the biological activity of human lens epithelial cells (HLEC)in producing inducible nitric oxide synthase (iNOS) and nitric oxide (NO),and toassesse the effect of diethyldithiocarbamate (DDC) on iNOS mRNA levels andexpression of ***:The human lens epithelial cell line SRA 01/04 wasused in this ***-quantitative reverse transcription polymerase chainreaction (RT-PCR) and Western blotting were used to detect,respectively,iNOSmRNA expression and protein ***:A costimulation by inter-feron gamma (IFN-γ) and lipopolysaccharide (LPS) was necessary for iNOS ex-pression in *** expression of iNOS was significantly reduced in a dose-dependent manner by adding DDC from 10 μmol/L to 1 mmol/***:The expression of iNOS in HLEC needs co-stimulation with IFN-γ and LPS and itis inhibited by DDC.

关键词： human lens epithelial cells inducible nitric oxide synthase interferon gamma lipopolysaccharide diethyldithiocarbamate

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Effect of the backbone structure of bidentate ligands in palladiumand nickel-catalyzed polar monomer copolymerization

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Science China Chemistry 2018年第11期61卷 1349-1350页

作者： Shingo ito Division of Chemistry and Biological Chemistry School of Physical and Mathematical Sciences Nanyang Technological University

Coordination-insertion copolymerization of non-polar ole- fins and polar monomers by late transition metal catalysts is a powerful method to synthesize functionalized polyolefins [1]. Seminal successes in this copolymerization have been achieved by using group-10 metal catalysts bearing a bidentate ligand such as phosphine-sulfonate and bisphosphine monoxide （BPMO）（Figure 1）.

关键词：金属催化剂梁结构镍氧化物

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Current advances in humanized mouse models

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Cellular & Molecular Immunology 2012年第3期9卷 208-214页

作者： Ryoji ito Takeshi Takahashi Ikumi Katano Mamoru ito Central Institute for Experimental Animals Kawasaki Japan

Humanized mouse models that have received human cells or tissue transplants are extremely useful in basic and applied human disease research. Highly immunodeficient mice, which do not reject xenografts and support cell and tissue differentiation and growth, are indispensable for generating additional appropriate models. Since the early 2000s, a series of immunodeficient mice appropriate for generating humanized mice has been successively developed by introducing the IL-2Rynull gene （e.g., NOD/SCID/7c＂uu and Rag2nu＂ycnun mice）. These strains show not only a high rate of human cell engraftment, but also generate well-differentiated multilineage human hematopoietic cells after human hematopoietic stem cell （HSC） transplantation. These humanized mice facilitate the analysis of human hematology and immunology in vivo. However, human hematopoietic cells developed from HSCs are not always phenotypically and functionally identical to those in humans. More recently, a new series of immunodeficient mice compensates for these disadvantages. These mice were generated by genetically introducing human cytokine genes into NODISCIDI？cn＂u and Rag2nulITcnUll mice. In this review, we describe the current knowledge of human hematopoietic cells developed in these mice. Various human disease mouse models using these humanized mice are summarized.

关键词： animal model immunodeficient mice immunology hematology humanized mice

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2,2′-Biazulene diimide-based conjugate polymers for highperformance field-effect transistors

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Science China Chemistry 2018年第8期61卷 973-974页

作者： Taku Shoji Shunji ito Graduate School of Science and Technology Shinshu University Graduate School of Science and Technology Hirosaki University

Azulene,which is one of the non-alternating 10π-electron aromatic compounds,has attracted the interest of many research groups due to the application to material sciences utilizing the unique optoelectronic properties.

关键词： Th THF Biazulene diimide-based conjugate polymers for highperformance field-effect transistors PTB

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Compelling Challenges in Antenna Technologies for Future Medical Applications

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Engineering 2022年第4期8卷 15-17页

作者： Koichi ito Center for Frontier Medical Engineering Chiba UniversityChiba 263-8522Japan

*** Numerous types of antennas have been developed,tested,and utilized for medical applications such as diagnosis,treatment,data transmission,and wireless power transfer(WPT).Recently,compact and robust antennas are needed to realize implantable and wearable wireless devices for medical applications such as delivering drugs from implanted capsules,stimulating nerve systems,and monitoring physiological data[1].Because antennas for medical applications are used close to or in a human body,their effects on the human body should be included in their development and evaluation[2].However,it is rather difficult due to ethical and practical reasons to employ a real human body when experimentally evaluating the characteristics of antennas or wireless devices for medical ***,numerical simulations are generally conducted as an alternative,using elaborate digital human models[3].Many different types of software are now commercially available for this purpose,along with digital human-body models.

关键词： antennas utilized diagnosis

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Field emission characteristics of nano-sheet carbon films deposited by quartz-tube microwave plasma chemical vapour deposition

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Chinese Physics B 2008年第4期17卷 1467-1471页

作者：顾广瑞金哲 ito Toshimichi Department of Physics College of ScienceYanbian University Division of Electrical Electronic and Information EngineeringGraduate School of EngineeringOsaka University

Nano-sheet carbon films are prepared on Si wafers by means of quartz-tube microwave plasma chemical vapour deposition （MPCVD） in a gas mixture of hydrogen and methane. The structure of the fabricated films is investigated by using field emission scanning electron microscope （FESEM） and Raman spectroscopy. These nano^carbon films are possessed of good field emission （FE） characteristics with a low threshold field of 2.6 V/μm and a high current density of 12.6 mA/cm^2 at an electric field of 9 V/μm. As the FE currents tend to be saturated in a high E region, no simple Fowler-Nordheim （F-N） model is applicable. A modified F N model considering statistic effects of FE tip structures and a space-charge-limited-current （SCLC） effect is applied successfully to explaining the FE data observed at low and high electric fields, respectively.

关键词： field emission carbon films nano-sheet microwave plasma chemical vapour deposition

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Imbricate structure of the Otakeyama area, Okayama Permian Yoshii Group in the Prefecture, southwest Japan

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Frontiers of Earth Science 2015年第1期9卷 152-163页

作者： Tsuyoshi ito Atsushi MATSUOKA State Key Laboratory of Geo-processes and Mineral Resources China University of Geosciences Wuhan 430074 China Department of Geology Faculty of Science Niigata University Niigata 950-2181 Japan

The Yoshii Group of the Akiyoshi terrane is distributed over the Otakeyama area, Okayama Prefecture, southwest Japan. The Yoshii Group is composed of siliceous rocks and clastics, comprising chert-clastic sequences. The Yoshii Group is divided into four structural units （Units Ot 1, Ot 2, Ot 3, and Ot 4） in structurally ascending order. Lithological similarities and radiolarian age suggest that these units exhibit almost identical ocean plate stratigraphy. These units are structurally-repeated to form an imbricate structure. This structure was possibly formed by off-scrape accretion at a toe of an accretionary complex. Additionally, a review of previous studies indicates that an imbricate structure is common in the Akiyoshi terrane. The presence of an imbricate structure in the Akiyoshi terrane, Permian accretionary complex, indicates the possibility that a specific horizon of Permian pelagic sequences acted as a drcollement zone.

关键词： accretionary complex imbricate structure,Permian radiolafia Yoshii Group Okayama Prefecture,southwest Japan

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