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Sucrose-associated SnRK1a1-mediated phosphorylation of Opaque2 modulates endosperm filling in maize

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Molecular Plant 2024年第5期17卷 788-806页

作者： Tao Yang Yunqin Huang Longyu Liao Shanshan Wang Haoyu Zhang Jingying pan Yongcai Huang Xiaoling Li Di Chen Tao Liu Xiaoduo Lu Yongrui Wu State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130SichuanChina National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai lnstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China Institute of Molecular Breeding for Maize Qilu Normal UniversityJinanChina

During maize endosperm filling,sucrose not only serves as a source of carbon skeletons for storage-reserve synthesis but also acts as a stimulus to promote this process.However,the molecular mechanisms underlying sucrose and endosperm filling are poorly understood.In this study,we found that sucrose promotes the expression of endosperm-filling hub gene Opaque2(O2),coordinating with storage-reserve accumulation.We showed that the protein kinase SnRK1a1 can attenuate O2-mediated transactivation,but sucrose can release this suppression.Biochemical assays revealed that SnRK1a1 phosphorylates O2 at serine 41(S41),negatively affecting its protein stability and transactivation ability.We observed that mutation of SnRK1a1 results in larger seeds with increased kernel weight and storage reserves,while overexpression of SnRK1a1 causes the opposite effect.Overexpression of the native O2(O2-OE),phospho-dead(O2-sa),and phospho-mimetic(O2-SD)variants all increased 100-kernel weight.Although O2-sa seeds exhibit smaller kernel size,they have higher accumulation of starch and proteins,resulting in larger vitreous endosperm and increased test weight.O2-SD seeds display larger kernel size but unchanged levels of storage reserves and test weight.O2-OE seeds show elevated kernel dimensions and nutrient storage,like a mixture of O2-sa and O2-SD seeds.Collectively,our study discovers a novel regulatory mechanism of maize endosperm filling.Identification of S41 as a SnRK1-mediated phosphorylation site in O2 offers a potential engineering target for enhancing storage-reserve accumulation and yield in maize.

关键词： maize Opaque2 SnRK1 endosperm phosphorylation sucrose

Geochronology and Geochemistry of Ore-related Granitoids in the Giant Gariatong Rb Deposit,Tibet and Implications for Rb Metallogeny in China

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Acta Geologica Sinica(English Edition) 2024年第1期98卷 83-103页

作者： LIN Bin TANG Juxing TANG pan SUN Yan QI Jing MsaNTOSH XIE Jinling DENG Shilin LI Faqiao XIE Fuwei ZHOU Aorigele MNR Key Laboratory of Metallogeny and Mineral Assessment Institute of Mineral ResourcesChinese Academy of Geological SciencesBeijing 100037China SinoProbe Laboratory Chinese Academy of Geological SciencesBeijing 100094China The Key Laboratory of the Ministry of Education on Solid Waste Treatment and Recycling School of Environment and ResourceSouthwest University of Science and TechnologyMianyangSichuan 621010China School of Earth Sciences and Resources China University of Geosciences(Beijing)Beijing 100083China Department of Earth Sciences University of AdelaideAdelaide SA-5005Australia Faculty of Geosciences and Environmental Engineering Southwest Jiaotong UniversityChengdu 610031China Regional Geological Survey Team of the Tibetan Bureau of Geology and Mineral Exploration and Development Lhasa 851400China College of Earth Science and Key Laboratory of Tectonic Controlled Mineralization and Oil Reservoirs Chengdu University of TechnologyChengdu 610059China

Rubidium(Rb)deposits mostly occur in the South China and Central Asia orogenic belts and are often closely associated with highly differentiated granites.This study investigates a newly-discovered giant Rb deposit at Gariatong in the Central Lhasa terrane in Tibet.Detailed field studies and logging data revealed that the Rb mineralization mainly occurs in monzogranite and is related to greisenization.LA-ICP-MS U-Pb dating of zircon yielded ages of 19.1±0.2 Ma and 19.0±0.2 Ma for greisenized monzogranite and fresh monzogranite,respectively.The monzogranites are characterized as strongly peraluminous,with high contents of SiO2,Al2O3,K2O and Na2O as well as a high differentiation index.They are enriched in light rare earth and large ion lithophile elements with significant negative Eu anomalies and depleted high fieldstrength elements.Petrological and geochemical features of these ore-related monzogranites suggest that they are highly fractionated S-type granites,derived from remelting of crustal materials in a post-collisional setting.The geochemistry of zircon and apatite points to a low oxygen fugacity of the ore-related monzogranite during the magma’s evolution.The discovery of the Gariatong Rb deposit suggests that the Central Lhasa terrane may be an important region for rare metal mineralization.

关键词： LA-ICP-MS U-Pb dating geochemistry Rb metallogeny Gariatong Tibet

电针对急性心肌梗死大鼠心肌电重构的影响及其机制研究

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针刺研究 2024年第2期49卷 155-163页

作者：萨喆燕潘晓华朱小香兰彩莲万隆罗来许金森福建省中医药科学院经络研究所福建省经络感传重点实验室福州350003

目的:探讨电针“内关”通过增强瞬时外向钾电流(Ito)改善急性心肌梗死大鼠心肌电重构的作用机制。方法:雄性SD大鼠随机分成对照组、模型组和电针组,每组10只。采用皮下注射异丙肾上腺素(85 mg/kg)诱导急性心肌梗死模型。电针组造模24 h... 详细信息

目的:探讨电针“内关”通过增强瞬时外向钾电流(Ito)改善急性心肌梗死大鼠心肌电重构的作用机制。方法:雄性SD大鼠随机分成对照组、模型组和电针组,每组10只。采用皮下注射异丙肾上腺素(85 mg/kg)诱导急性心肌梗死模型。电针组造模24 h后电针左侧“内关”,每次20 min,每日1次,连续5 d。TTC染色观察心肌坏死情况;HE染色观察心肌组织病理形态、测量心肌细胞横截面积;心电图记录ST段高度、QT间期(QT)、校正QT间期(QTc),计算QT间期离散率(QTd)和校正QT间期离散率(QTcd);RNA测序法检测心肌组织钾离子通道相关基因表达差异;实时荧光定量PCR法和Western blot法分别检测心肌组织Kchip2、Kv4.2的mRNA和蛋白表达。结果:TTC染色可见对照组心肌呈红色,模型组大鼠心肌大部分呈白色缺血样改变,电针组仅见心尖处心肌组织呈白色。与对照组比较,模型组大鼠心肌细胞横截面积显著增大(P<0.01),ST段显著抬高(P<0.01),QT、QTc、QTd和QTcd均显著增加(P<0.05,P<0.01);与模型组比较,电针组大鼠心肌细胞横截面积显著减小(P<0.01),ST段显著下降(P<0.01),QT、QTc、QTcd和QTd均显著减少(P<0.01,P<0.05)。RNA测序共筛选出20个3组共同表达的钾离子通道相关基因,电针组与模型组比较,其中Kchip2上调差异倍数最大。与对照组比较,模型组大鼠心肌组织Kchip2、Kv4.2 mRNA和蛋白表达均显著降低(P<0.01,P<0.05);与模型组比较,电针组大鼠心肌组织Kchip2、Kv4.2 mRNA和蛋白表达均显著升高(P<0.01,P<0.05)。结论:电针可改善心肌梗死大鼠心肌电重构,其机制可能与增加Ito主要成分Kchip2、Kv4.2表达有关。

关键词：电针心肌梗死心肌电重构瞬时外向钾电流

温肾化痰调经方联合西药对肾虚痰湿型多囊卵巢综合征患者生殖激素、糖脂代谢的影响研究

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世界临床药物 2024年第6期45卷 648-653页

作者：潘飒肖蕾王华大庆龙南医院齐齐哈尔医学院第五附属医院检验科黑龙江大庆163453 大庆龙南医院齐齐哈尔医学院第五附属医院内分泌科黑龙江大庆163453

目的探讨温肾化痰调经方联合西医常规疗法对肾虚痰湿型多囊卵巢综合征(polycystic ovary syndrome,PCOS)患者生殖激素、糖脂代谢的影响。方法选择2022年1月至2023年1月我院收治的肾虚痰湿型PCOS患者96例,按简单排序随机分组,分为观察组... 详细信息

目的探讨温肾化痰调经方联合西医常规疗法对肾虚痰湿型多囊卵巢综合征(polycystic ovary syndrome,PCOS)患者生殖激素、糖脂代谢的影响。方法选择2022年1月至2023年1月我院收治的肾虚痰湿型PCOS患者96例,按简单排序随机分组,分为观察组(炔雌醇环丙孕酮+二甲双胍+温肾化痰调经方)、对照组(炔雌醇环丙孕酮+二甲双胍),每组48例,以连续治疗21 d、停药7 d为1个疗程,两组均连续治疗3个疗程。比较两组临床疗效、黄体生成素(luteinizing hormone,LH)、LH/卵泡雌激素(follicular estrogen,FSH)、抗苗勒试管激素(anti-muller test tube hormone,AMH)、抑制素(inhibin,INH)B、糖化血红蛋白(glycosylated hemoglobin,Hb)A1c、总胆固醇(total cholesterol,TC)、三酰甘油(triacylglycerol,TG)及稳态模型评估胰岛素抵抗指数(homeostasis model assessment-insulin resistance,HOMA-IR)等。结果观察组临床总有效率高于对照组(89.58%vs 62.50%,P0.05)。结论温肾化痰调经方联合西医常规疗法对肾虚痰湿型PCOS患者疗效明显,有助调节生殖激素和糖脂代谢指标。

关键词：温肾化痰调经方肾虚痰湿型多囊卵巢综合征生殖激素糖脂代谢性激素

NF-κB介导VEGF-A促进食管鳞状细胞癌血管生长和细胞增殖的实验研究

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川北医学院学报 2024年第5期39卷 583-588页

作者：潘冠华阿迪力·萨来吕红博新疆医科大学附属肿瘤医院胸外科二病区新疆乌鲁木齐830054

目的:探讨NF-κB、VEGF-A在食管鳞状细胞癌(ESCC)患者癌组织中的表达情况及NF-κB与VEGF-A在促进食管鳞癌血管生成和细胞增殖的关系。方法:收集32例行食管癌切除术患者食管鳞癌组织及癌旁组织标本,蛋白印迹法(Western blot)检测癌及癌... 详细信息

目的:探讨NF-κB、VEGF-A在食管鳞状细胞癌(ESCC)患者癌组织中的表达情况及NF-κB与VEGF-A在促进食管鳞癌血管生成和细胞增殖的关系。方法:收集32例行食管癌切除术患者食管鳞癌组织及癌旁组织标本,蛋白印迹法(Western blot)检测癌及癌旁组织中NF-κB、VEGF-A的蛋白表达情况;Pearson相关系数分析NF-κB与VEGF-A的相关性。在体外实验中过表达和敲低食管鳞癌细胞中的NF-κB后,通过CCK-8、平板克隆实验、流式细胞术检测NF-κB对肿瘤细胞恶性表型的影响。Western blot检测不同NF-κB表达水平对VEGF-A及VEGF-R2的表达水平的变化。结果:NF-κB、VEGF-A在癌组织中高表达,在癌旁组织中低表达。过表达和敲低NF-κB对细胞的增殖分别有促进和抑制作用、对细胞的凋亡有抑制和促进作用、对细胞周期的G1/S转换有促进和抑制作用。过表达和敲低NF-κB后VEGF-A、VEGF-R2的表达也随之升高和降低。结论:ESCC组织中的NF-κB与VEGF-A表达高于癌旁组织,二者正相关,且NF-κB通过过表达VEGF-A对ESCC血管生成和细胞增殖具有正向促进作用。

关键词：食管鳞状细胞癌核转录因子-κB 血管生成因子细胞增殖

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紫斑牡丹花粉对D-半乳糖致衰老模型小鼠的影响

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中国药理学通报 2024年第4期40卷 730-739页

作者：彭腾腾王信李海燕尹盼盼范彬石晓峰甘肃省医学科学研究院甘肃兰州730050 甘肃中医药大学药学院甘肃兰州730030

目的探究紫斑牡丹花粉对D-半乳糖致衰老小鼠学习记忆力及氧化应激损伤的影响。方法60只C57BL/6小鼠随机分为空白组、模型组、阳性组、紫斑牡丹花粉组(750、1500、3000 mg·kg^(-1)),采用颈背部皮下注射D-半乳糖(500 mg·kg^(-1)),制备... 详细信息

目的探究紫斑牡丹花粉对D-半乳糖致衰老小鼠学习记忆力及氧化应激损伤的影响。方法60只C57BL/6小鼠随机分为空白组、模型组、阳性组、紫斑牡丹花粉组(750、1500、3000 mg·kg^(-1)),采用颈背部皮下注射D-半乳糖(500 mg·kg^(-1)),制备亚急性衰老模型。Morris水迷宫评估小鼠学习记忆力;HE、DAPI染色评估肝、脑组织细胞损伤情况;ELIsa法检测小鼠血清、肝脑组织内MDA、T-AOC、GSH-Px、SOD、CAT及γ-H2AX、sa-β-Gal水平。结果水迷宫实验结果表明,紫斑牡丹花粉能明显提高衰老小鼠的学习记忆力。组织染色结果表明,紫斑牡丹花粉对D-半乳糖致衰老小鼠肝脑组织细胞损伤具有保护作用,且高剂量组作用最为明显。ELIsa结果显示,与模型组比较,紫斑牡丹花粉各剂量组的MDA、γ-H2AX、sa-β-Gal水平降低,T-AOC、GSH-Px、SOD、CAT水平升高。结论紫斑牡丹花粉能改善D-半乳糖致衰老模型小鼠的学习记忆力,提高衰老小鼠体内抗氧化酶活性和清除自由基、脂质过氧化物的能力,保护肝脑脏器组织细胞免受氧化损伤,提示其具有潜在的延缓衰老的作用。

关键词：紫斑牡丹花粉 D-半乳糖氧化应激抗衰老 Morris水迷宫 HE染色 DAPI染色

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基于Spark的双阶段sa及GA求解MTSP

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郑州大学学报（工学版） 2024年第4期45卷 62-69,94页

作者：孙鉴刘品李昊陈攀北方民族大学计算机科学与工程学院宁夏银川750021 北方民族大学图像图形智能处理国家民委重点实验室宁夏银川750021

针对总路径长度最小的单站点多旅行商问题,提出了基于Spark的模拟退火和遗传算法结合的两阶段KsaGA算法。在第一阶段,通过k-means聚类将多旅行商问题拆分为多个单旅行商问题,并使用模拟退火算法对组内城市的遍历次序进行优化。在第二阶... 详细信息

针对总路径长度最小的单站点多旅行商问题,提出了基于Spark的模拟退火和遗传算法结合的两阶段KsaGA算法。在第一阶段,通过k-means聚类将多旅行商问题拆分为多个单旅行商问题,并使用模拟退火算法对组内城市的遍历次序进行优化。在第二阶段,通过遗传算法对城市的分组进行优化,并基于染色体分组编码方式设计了交叉、变异算子以及混合局部优化算子,以提高算法的搜索空间和收敛速度。随着城市数量的增加,计算规模变大,利用遗传算法的特性实现算法的并行,以加快算法运行效率。最后,通过选取TSPLIB的部分数据集进行仿真实验,将KsaGA与ACO、GA、SPKsa、ALNS和NSGA-Ⅱ的求解质量以及GA和NSGA-Ⅱ的收敛速度进行对比。研究结果表明:KsaGA在解决单站点多旅行商问题时能够快速收敛,并且相较于其他算法,求解质量得到了很大提升。同时,随着城市数量和旅行商数量增加,KsaGA的优势更为明显。

关键词：多旅行商问题并行遗传算法分组编码局部优化算子

Effects of electroacupuncture on rats with cognitive impairment:An iTRAQ-based proteomics analysis

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Journal of Integrative Medicine 2023年第1期21卷 89-98页

作者： Zhe-yan sa Jin-sen Xu Xiao-hua pan Shu-xia Zheng Qian-ru Huang Long Wan Xiao-xiang Zhu Cai-lian Lan Xiao-ran Ye Department of Meridian Research Fujian Academy of Chinese Medical SciencesFuzhou 350003Fujian ProvinceChina Key Laboratory of Propagated Sensation along Meridian of Fujian Province Fuzhou 350003Fujian ProvinceChina

Objective The study explores the effects of electroacupuncture(EA)at the governing vessel(GV)on proteomic changes in the hippocampus of rats with cognitive impairment.Methods Healthy male rats were randomly divided into 3 groups:sham,model and EA.Cognitive impairment was induced by left middle cerebral artery occlusion in the model and EA groups.Rats in the EA group were treated with EA at Shenting(GV24)and Baihui(GV20)for 7 d.Neurological deficit was scored using the Longa scale,the learning and memory ability was detected using the Morris water maze(MWM)test,and the proteomic profiling in the hippocampus was analyzed using protein-labeling technology based on the isobaric tag for relative and absolute quantitation(iTRAQ).The Western blot(WB)analysis was used to detect the proteins and validate the results of iTRAQ.Results Compared with the model group,the neurological deficit score was significantly reduced,and the escape latency in the MWM test was significantly shortened,while the number of platform crossings increased in the EA group.A total of 2872 proteins were identified by iTRAQ.Differentially expressed proteins(DEPs)were identified between different groups:92 proteins were upregulated and 103 were downregulated in the model group compared with the sham group,while 142 proteins were upregulated and 126 were downregulated in the EA group compared with the model group.Most of the DEPs were involved in oxidative phosphorylation,glycolipid metabolism and synaptic transmission.Furthermore,we also verified 4 DEPs using WB technology.Although the WB results were not exactly the same as the iTRAQ results,the expression trends of the DEPs were consistent.The upregulation of heat-shock proteinβ1(Hspb1)was the highest in the EA group compared to the model group.Conclusion EA can effect proteomic changes in the hippocampus of rats with cognitive impairment.Hspb1 may be involved in the molecular mechanism by which acupuncture improves cognitive impairment.

关键词： Cerebral ischemia Vascular cognitive impairment Electroacupuncture Proteomics Isobaric tag for relative Absolute quantitation

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光热响应型控释微球的可控制备及其性能

化工进展 2024年第3期43卷 1474-1483页

作者：杨嘉琪巨晓洁谢锐汪伟刘壮潘大伟褚良银四川大学化学工程学院四川成都610065 四川大学高分子材料工程国家重点实验室四川成都610065

单一的光热治疗(PTT)效果有限,往往不能彻底治愈肿瘤。随着材料科学与生物医学的融合,多功能药物载体材料得到了很好的开发利用,有助于将PTT与其他治疗方法联合使用,为协同增强抗肿瘤疗效提供了有效的策略。本研究以载有吲哚菁绿(ICG)... 详细信息

单一的光热治疗(PTT)效果有限,往往不能彻底治愈肿瘤。随着材料科学与生物医学的融合,多功能药物载体材料得到了很好的开发利用,有助于将PTT与其他治疗方法联合使用,为协同增强抗肿瘤疗效提供了有效的策略。本研究以载有吲哚菁绿(ICG)的聚乳酸-羟基乙酸共聚物(PLGA)纳米粒子为光热剂,以聚乙烯醇(PVA)/海藻酸钠(sa)为载体基材,采用微流控技术可控制备了一种新型光热响应型控释微球(PLGAICG@PVA/sa)。系统研究了微球的形貌尺寸可控性、光热转化性能、机械性能和生物相容性,并以盐酸阿霉素(DOX)为模型药物,探讨了该微球载体对DOX的负载能力和光热响应性控释能力。结果表明,所制备的PLGAICG@PVA/sa微球具有良好的单分散性,表现出优异的光热转换效应,0.5W/cm^(2)近红外光照射15min的温度增量为18.5℃且稳定性良好;微球亦具有良好的可压缩性和弹性性能,其杨氏模量为317.0kPa。在模拟生理环境中,微球中DOX药物的释放行为符合一级释放动力学模型并具有明显的光热刺激响应性。该微球材料在药物控释及肿瘤的光热/化疗联合治疗等领域具有广泛的应用前景。

关键词：微流体学光热治疗复合微球吲哚菁绿纳米粒子药物释放弹性