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Epigenetic changes associated with oocyte aging

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Science China(Life Sciences) 2012年第8期55卷 670-676页

作者： SCHATTEN Heide MA JunYu SCHATTEN Heide sun qingyuan State Key Laboratory of Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Department of Animal Sciences Chungbuk National UniversityCheongju 361-763 Republic of Korea Department of Veterinary Pathoiology Vniversity of MissouriColumbiaMO 65211USA

It is well established that the decline in female reproductive outcomes is related to postovulatory aging of oocytes and advanced maternal *** oocyte quality is correlated with compromised genetic integrity and epigenetic changes during the oocyte aging ***,we review the epigenetic alterations,mainly focused on DNA methylation,histone acetylation and methylation associated with postovulatory oocyte aging as well as advanced maternal ***,we address the underlying epigenetic mechanisms that contribute to the decline in oocyte quality during oocyte aging.

关键词： fertility advanced maternal age postovulatory oocyte aging DNA methylation histone modification

Roles of MAP kinase signaling pathway in oocyte meiosis

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Chinese Science Bulletin 2002年第14期47卷 1157-1162页

作者： Hengyu Fan Chao Tong Dayuan Chen qingyuan sun Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China

Mitogen-activated protein kinase (MAPK) is a family of Ser/Thr protein kinases expressed widely in eu-karyotic cells. MAPK is activated by a cascade of protein kinase phosphorylation and plays pivotal roles in regulating meiosis process in oocytes. As an important physical substrate of MAPK, p90rsk mediates numerous MAPK functions. MAPK was activated at G2/M transition during meiosis. Its activity reached the peak at M I stage and maintained at this level until the time before the pronuclear formation after fertilization. There is complex interplay between MAPK and MPF in the meiosis regulation. Furthermore, other intracel-lular signal transducers, such as cAMP, protein kinase C and protein phosphotase, ect., also regulated the activity of MAPK at different stages during meiosis in oocytes. In the present article, the roles of MAPK signaling pathway in oo-cyte meiosis are reviewed and discussed.

关键词： protein kinase oocyte meiosis signal transduction fer-tilization.

Sperm antigen MSH27 participates in sperm-egg membrane fusion

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Chinese Science Bulletin 1999年第16期44卷 1483-1489页

作者： Dazhong Zhuang Xiangfen Song Guojun Hu qingyuan sun Dayuan Chen Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China

A monoclonal antibody (mAb), MSH27 has been selected from an mAb library which has been prepared with separated mouse sperm heads as antigens. Three aspects of evidence indicated that the MSH27 antigen was involved in the process of sperm-egg membrane fusion. After the acrosome reaction, the antigen was located at sperm equatorial segment and in postacrosomal area, where, it was widely accepted, the sperm-egg membrane fusion

关键词： spermatozoon membrane fusion monoclonal antibody antigen.

MAPK regulates cell cycle progression in pig oocytes and fertilized eggs

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Chinese Science Bulletin 2002年第10期47卷 843-847,882页

作者： LI Manyu FAN Hengyu TONG Chao CHEN Dayuan XIA Guoliang sun qingyuan State Key Laboratory of Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100080China College of Biological Sciences China Agricultural University College of Veterinary Medicine China Agricultural UniversityBeijing 100094China

Oocytes collected from prepubertal gilt ovaries were matured in vitro (IVM), and fertilized in vitro (IVF) or electrically activated. Phosphorylation of mitogen-activated protein kinase (MAPK) was detected with SDS-PAGE and Western blotting, and translocation of ERK2 was observed with immunofluorescent cytochemistry. We found that the quantity of MAPK kept unchanged during oocyte maturation. There was no phosphorylated MAPK in porcine oocytes at the germinal vesicle (GV) stage; a little MAPK was phosphorylated at 20 h of IVM; a high level phosphory-lation was detected at 30 h, while MAPK phosphorylation decreased at 36 h; and then MAPK phosphorylation increased again to the peak level from 40 to 60 h. ERK2 translocated from the peripheral cytoplasm to inner cytoplasm and nuclear area during oocyte maturation. There was nearly no phosphorylated MAPK at 18 and 20 h of electrically activated oocytes, but phosphorylation increased at 22 h. There was no phosphorylated MAPK at 12 h of IVF, while

关键词： porcine oocyte MAPK IVM IVF.

The roles of Septin 7 in mouse oocyte meiosis

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The roles of Septin 7 in mouse oocyte meiosis

Nuclear transfer using nonquiescent adult fibroblasts from a bovine ear

中华医学会第六次全国生殖医学学术会议

作者： Sen Li qingyuan sun Peking University Peoples Hospital Institute of Zoology Chinese Academy of Science

Septin 7 is a conserved GTP-binding *** this study,we examined the localization and functions of Septin 7 using mouse oocyte meiotic *** analysis showed that ERK3 localized to the spindles from the pre-MI stage to the Mil *** of Septin7 by siRNA microinjection caused abnormal spindles and affected extrusion of the first polar *** 7 mRNA tagged with myc was injected into GV stage oocytes to overexpress Septin ***-Septin 7 localized to the spin-

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Chinese Science Bulletin 1999年第21期44卷 1971-1974页

作者： Jilong Liu Minkang Wang Li Lian Jinsong Li Shaorong Gao Zhiming Han qingyuan sun Xiangfen Song Dongmei Zhang Yihai Li et al. Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China Yunnan Educ Coll Kunming 650223 Peoples R China Yangzhou Univ Coll Vet Sci & Anim Husb Yangzhou 225009 Peoples R China Beijing Normal Univ Dept Biol Beijing 100875 Peoples R China Tianjin Inst Anim Husb & Vet Sci Tianjin 300112 Peoples R China

The natural reproduction of mammal is sexual reproduction, which needs fertilization involving sperm and oocyte. Nuclear transfer provided an asexual reproduction method for mammal. Donor cells used in previous experiments of nuclear transfer were mostly from undif-ferentiated or non-terminally differentiated cells, such as embryonic or fetal cells. However, since Wilmut et al. obtained a viable lamb by transfer of an adult sheep somatic cell into

关键词： nonquiescent phase adult cattle fibroblast nuclear transfer.

Serial nuclear transfer improves the development of interspecies reconstructed giant panda (Aluropoda melanoleucd) embryos

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Chinese Science Bulletin 2002年第6期47卷 467-469页

作者： LI Jinsong CHEN Dayuan HAN Zhiming ZHU Ziyu WEN Duancheng sun qingyuan LIU Zhonghua WANG Minkang LIAN Li DU Jun WANG Pengyan ZHANG Hemin State Key Laboratory of Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100080China College of Animal Science and Veterinary Medicine Yangzhou UniversityYangzhou 225009China College of Life Science Beijing Normal UniversityBeijing 100857China Suzhou University Suzhou 215151China Northeast Agriculture University Harbin 150030China Yunnan University Kunming 650223China China Research and Conservation Center for Giant Panda Wolong Nature ReserveWenchuan 623006China

Interspecies somatic nuclear transfer (NT) may provide a new approach for preservation of the endangered rare species. Previous interspecies cloning studies have shown that a nucleus from a quiescent somatic cell supports early development of reconstructed embryos in the ooplasm from another species. In this study, we transferred nonqui-escent somatic cells from a giant panda into the perivitelline space of the enucleated rabbit oocytes. After electrofusion (at the rate of 71.6%) and electrical activation, 4.2% of the panda-rabbit reconstructed embryos developed to blastocyst in vitro. For improving the development rate of reconstructed embryos, we used serial NT in this study, i.e. blas-tomeres from reconstructed morulae were transferred into the perivitelline space of the enucleated rabbit oocytes. The fusion rates in the groups of serial I, serial Ⅱ and serial Ⅲ were 79.5%, 84.1% and 78.0%, respectively, having no difference with that of somatic group. And the blastocyst rates in serial NT groups

关键词： nonquiescent cell giant panda rabbit interspecies nu-clear transfer serial nuclear transfer.

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橘黄酮的提取及其抑菌特性

化工进展 2016年第8期35卷 2528-2532页

作者：赵悦孙庆元孙琦大连工业大学生物工程学院辽宁大连116034

采用乙醇浸提法从橘子皮中提取黄酮,通过正交试验确定提取的最佳条件为:70%乙醇为提取剂,固液比为1︰20(g/m L),提取温度是70℃,提取时间2h,在510nm处测定橘黄酮质量浓度为0.205g/L,从橘子皮中提取的黄酮干重得率为2.938mg/g。本实验探... 详细信息

采用乙醇浸提法从橘子皮中提取黄酮,通过正交试验确定提取的最佳条件为:70%乙醇为提取剂,固液比为1︰20(g/m L),提取温度是70℃,提取时间2h,在510nm处测定橘黄酮质量浓度为0.205g/L,从橘子皮中提取的黄酮干重得率为2.938mg/g。本实验探究橘黄酮的抑菌活性,采用牛津杯法测抑菌作用,结果表明,橘黄酮对金黄色葡萄球菌、大肠杆菌、黑曲霉、灰葡萄孢菌和黄枝孢菌均有抑制作用,铜和镁离子可分别增强橘黄酮对细菌和真菌的抑菌作用,低p H和低温可提高抑菌效果,且优化p H和温度的橘黄酮剂型降低了最小抑菌浓度(MIC)20%,对金黄色葡萄球菌、大肠杆菌、黑曲霉、灰葡萄孢菌和黄枝孢菌的抑菌作用分别提高了17%、27%、25%、28%、19%,高于对氯间二甲苯酚和甲基托布津的抑菌作用,特别是高于对细菌的抑菌作用。

关键词：浸取橘皮黄酮抑菌优化生物技术

Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda

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Chinese Science Bulletin 2000年第20期45卷 1883-1885页

作者： DING Bo SHI Peng XIANGYU Jinggong ZHANG Yaping CHEN Dayuan sun qingyuan LI Guangpeng WANG Minkang LIU Yilong KANG Li HAN Zhiming SONG Xiangfen LI Jingsong CHEN Yuchun Laboratory of Cellular and Molecular Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China State Key Laboratory of Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100080China Fuzhou Panda Institute Center Fuzhou 350001China 不详

A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study. Total DNA was prepared from interspecies reconstructed blastocyst and a giant panda specific microsatellite locus g010 was successfully amplified. DNA sequencing of the PCR product showed that two sequences of reconstructed blastocysts are the same as that of positive control giant panda. Our results prove that the nucleus of interspecies reconstructed blastocyst comes from somatic nucleus of donor giant panda.

关键词： giant panda (Ailuropoda melanoleuca) interspecies cloning microsatellite DNA nucleus.