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Modeling the regional grazing impact on vegetation carbon sequestration ability in Temperate Eurasian Steppe

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Journal of Integrative Agriculture 2017年第10期16卷 2323-2336页

作者： CHEN Yi-zhao sun Zheng-guo qin Zhi-hao Pavel Propastin WANG Wei LI Jian-long RUANHong-hua Joint Innovation Center for Modern Forestry Studies College of Biology and the Environment Nanjing Forestry University Nanjing 210037 P.R.China School of Life Science Nanjing University Nanjing 210093 P.R.China College of Prataculture Science Nanjing Agricultural University Nanjing 210095 P.R. China Institute of Agro-resources and Regional Planning Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China Institute of Geography Georg-August University GottingenGottingen 37077 Germany Department of Bioclimatology Busgen-lnstitute Georg-August University Gottingen Gottingen 37077 Germany College of Forestry Nanjing Forestry University Nanjing 210037 P.R.China

Anthropogenic activities profoundly influence carbon sequestration in the Eurasian Steppe. In particular, grazing has been identified as having a major effect on carbon sequestration. However, the extent to which grazing affects regional patterns or carbon sequestration is unknown. In this study, we evaluated the impact of regional grazing on grassland carbon seques- tration using the Boreal Ecosystem Productivity Simulator （BEPS） and the Shiyomi grazing model. Model performances were validated against the results from field measurements and eddy covariance （EC） sites. Model outputs showed that in 2008, the regional net primary productivity （NPP） was 79.5 g C m-2, and the net ecosystem productivity （NEP） was -6.5 g C m-2, characterizing the region as a Weak carbon source. The Mongol Steppe （MS） was identified as a carbon sink, whereas the Kazakh Steppe （KS） was either carbon neutral or a weak carbon source. The spatial patterns of grazing density are divergent between the MS and the KS. in the MS, livestock was mainly distributed in China with relatively good management, while in the KS livestock was mainly concentrated in the southern countries （especially Uzbekistan and Turkmenistan） with harsh environments and poor management. The consumption percentages of NPP in Turkmenistan, Tajikistan and Uzbekistan were 5.3, 3.3 and 1.2%, respectively, whereas the percentages in other countries were lower than 1%. Correspondingly, grazing consumption contributed to the carbon sources of Turkmenistan, Tajikistan and Uzbekistan by 11.6, 6.3 and 4.3%, respectively, while it weakened the carbon sink in Inner Mongolia, China and Mongolia by 1.6 and 0.5%. This regional pattern should be affected by different sub-regional characteristics, e.g., the continuous degradation of grassland in the southern part of the KS and the restoration of grassland in Inner Mongolia, China.

关键词： Temperate Eurasian Steppe terrestrial modeling carbon sequestration ability grazing activity regional evaluation

延迟投饵和不同开口饵料对中华花鳅仔鱼生长的影响

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西华师范大学学报（自然科学版） 2024年第4期45卷 361-366页

作者：杨骏张云德邹成坤梁勤彪孙治宇广元市林业局四川广元628000 广元市利州区林业局四川广元628000 广元市林业科学研究院四川广元628000 四川省林业科学研究院成都610081

为积累中华花鳅(Cobitis sinensis)人工繁育技术基础资料,通过人工繁殖得到中华花鳅仔鱼,探究延迟投饵和不同开口饵料对其死亡率和生长的影响,同时测定了中华花鳅仔鱼的初次摄食率和饥饿不可逆点。实验结果表明:仔鱼出膜1.5 d后开口摄食... 详细信息

为积累中华花鳅(Cobitis sinensis)人工繁育技术基础资料,通过人工繁殖得到中华花鳅仔鱼,探究延迟投饵和不同开口饵料对其死亡率和生长的影响,同时测定了中华花鳅仔鱼的初次摄食率和饥饿不可逆点。实验结果表明:仔鱼出膜1.5 d后开口摄食,初次摄食率为85%,2.5 d达到100%,5.0 d后下降;饥饿不可逆点发生在8.5~9.0 d;中华花鳅仔鱼的最适投饵时间应为仔鱼孵化后1.5 d,可以选择鸡蛋黄悬浊液作为中华花鳅仔鱼开口饵料。

关键词：中华花鳅仔鱼延迟投饵开口饵料生长

消风止痒颗粒通过降低白三烯水平对小鼠特应性皮炎急性瘙痒的治疗作用研究

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药学实践与服务 2024年第5期42卷 211-216页

作者：丁华敏郭羽晨秦春霞宋志兵孙莉莉上海市浦东新区浦南医院上海200125 安徽中医药大学药学院安徽合肥230012

目的建立小鼠特应性皮炎急性瘙痒模型,研究消风止痒颗粒的止痒作用及其机制探讨。方法采用诱导法制备特应性皮炎小鼠模型。小鼠每天先后用卡泊三醇和卵清蛋白(OVA)涂抹右耳致敏10 d,然后在小鼠右颊皮内注射OVA进行激发,致使小鼠出现急... 详细信息

目的建立小鼠特应性皮炎急性瘙痒模型,研究消风止痒颗粒的止痒作用及其机制探讨。方法采用诱导法制备特应性皮炎小鼠模型。小鼠每天先后用卡泊三醇和卵清蛋白(OVA)涂抹右耳致敏10 d,然后在小鼠右颊皮内注射OVA进行激发,致使小鼠出现急性瘙痒,记录小鼠激发后30~60 min的抓挠次数。实验分为5组:空白对照组、模型组、消风止痒颗粒低剂量组(7.2 g/kg)和高剂量组(14.4 g/kg)、阳性对照组(孟鲁司特5 mg/kg)。各组于激发前12 h及30 min各灌胃给药一次。通过Elisa检测小鼠血清中白三烯水平,通过流式细胞术检测血液中嗜碱性粒细胞比例及激活状态。结果小鼠经致敏和激发后出现明显的抓挠行为,模型组小鼠激发后30~60 min内抓挠次数均值为56次,消风止痒颗粒低和高剂量组小鼠抓挠次数均值分别为42次和23次,较模型组均显著降低(P<0.05)。消风止痒颗粒低、高剂量组血清中白三烯水平和嗜碱性粒细胞比例与模型组比较,均明显降低(P<0.05)。结论消风止痒颗粒对小鼠特应性皮炎急性瘙痒具有一定治疗作用,作用机制与其降低特应性皮炎小鼠血清中白三烯水平和降低嗜碱性粒细胞比例有关。

关键词：消风止痒颗粒特应性皮炎瘙痒嗜碱性粒细胞白三烯

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文本生成图像研究综述

浙江大学学报（工学版） 2024年第2期58卷 219-238页

作者：曹寅秦俊平马千里孙昊闫凯王磊任家琪内蒙古工业大学数据科学与应用学院内蒙古呼和浩特010000 内蒙古自治区基于大数据的软件服务工程技术研究中心内蒙古呼和浩特010000

对文本生成图像任务进行综合评估和整理,根据生成图像的理念,将文本生成图像任务分为3大类:基于生成对抗网络架构生成图像、基于自回归模型架构生成图像、基于扩散模型架构生成图像.针对基于生成对抗网络架构的文本生成图像方法,按照改... 详细信息

对文本生成图像任务进行综合评估和整理,根据生成图像的理念,将文本生成图像任务分为3大类:基于生成对抗网络架构生成图像、基于自回归模型架构生成图像、基于扩散模型架构生成图像.针对基于生成对抗网络架构的文本生成图像方法,按照改进的不同技术点归纳为6小类:采用多层次体系嵌套架构、注意力机制的应用、应用孪生网络、采用循环一致方法、深度融合文本特征和改进无条件模型.通过对不同方法的分析,总结并讨论了现有的文本生成图像方法通用评估指标和数据集.

关键词：人工智能生成内容文本生成图像生成对抗网络自回归模型扩散模型

Application and verification of simultaneous determination of cellulose δ^(13)C and δ^(18)O in Picea shrenkiana tree rings from northwestern China using the high-temperature pyrolysis method

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Journal of Arid Land 2018年第6期10卷 864-876页

作者： XU Guobao LIU Xiaohong sun Weizhen CHEN Tuo ZHANG Xuanwen ZENG Xiaomin WU Guoju WANG Wenzhi qin Dahe State Key Laboratory of Cryospheric Science Northwest Institute of Eco-Environment and Resources Chinese Academy of Sciences School of Geography and Tourism Shaanxi Normal University Department of Geology and Earth System Science Interdisciplinary Center University of Maryland Institute of Mountain Hazards and Environment Chinese Academy of Sciences

Stable isotopes in tree-ring cellulose provide important data in ecological,archaeological,and paleoenvironmental researches,thereby,the demand for stable isotope analyses is increasing *** measurement of cellulose δC and δO values from tree rings would reduce the cost of isotopic commodities and improve the analytical efficiency compared with conventional separate *** this study,we compared the δC and δO values of tree-ringα-cellulose from Tianshan spruce(Picea schrenkiana)in an arid site in the drainage basin of the Urumqi River in Xinjiang of northwestern China based on separate and simultaneous measurements,using the combustion method(at1050°C)and the high-temperature pyrolysis method(at 1350°C and 1400°C).We verified the results of simultaneous measurement using the outputs from separate measurement and found that both methods(separate and simultaneous)produced similar δC *** two-point calibrated method improved the results(range and variation)of δC and δO *** mean values,standard deviations,and trends of the tree-ring δC obtained by the combustion method were similar to those by the pyrolysis method followed by two-point *** simultaneously measured δO from the pyrolysis method at 1400°C had a nearly constant offset with that the pyrolysis method at 1350°C due to isotopic-dependence on the reaction ***,they showed similar variations in the time *** climate responses inferred from simultaneously and separately measured δC and δO did not differ between the two *** tree-ring δC and δO values were negatively correlated with standardized precipitation evapotranspiration index from May to *** addition,the δO was significantly correlated with temperature(positive),precipitation(negative),and relative humidity(negative)from May to *** tree-ring δC and δO values determined simultaneously through the high-temperature pyrolysis method could produce acceptable and reliable sta

关键词： tree rings stable carbon and oxygen isotopes combustion pyrolysis simultaneous determination calibration Picea schrenkiana

基于CONSORT和STRICTA评价针刺治疗慢性阻塞性肺疾病随机对照试验质量的研究

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成都中医药大学学报 2024年第3期47卷 74-80页

作者：李茜罗钦余欣陈明孙铭声余曙光杨莎成都中医药大学针灸推拿学院四川成都610072 攀枝花学院四川攀枝花617000 四川省针灸临床医学研究中心四川成都610072 成都中医药大学针灸脑科学研究中心四川成都610072

目的:采用Cochrane协作网RCT偏倚风险评价工具(RoB2)、临床试验报告统一标准(CONSORT)和针刺临床试验干预措施报告标准(STRICTA)评价针刺治疗慢性阻塞性肺疾病(Chronic Obstructive Pulmonary Disease,COPD)随机对照试验的质量,为提高... 详细信息

目的:采用Cochrane协作网RCT偏倚风险评价工具(RoB2)、临床试验报告统一标准(CONSORT)和针刺临床试验干预措施报告标准(STRICTA)评价针刺治疗慢性阻塞性肺疾病(Chronic Obstructive Pulmonary Disease,COPD)随机对照试验的质量,为提高临床证据质量提供参考。方法:计算机检索中国知网(CNKI)、维普中文期刊服务平台(VIP)、万方数据知识服务平台(WanFang)、中国生物医学文献数据库(CBM)以及PubMed、Cochrane Library、EmBase收录的关于针刺治疗COPD的随机对照试验,检索时限从建库开始到2022年2月28日。应用RoB2、CONSORT和STRICTA对纳入文献的方法学和报告质量进行评价,评价结果的一致性用kappa值表示。结果:最终纳入文献51篇,其中风险偏倚为“低风险”的3篇(5.9%),“不确定”的26篇(51.0%),“高风险”的22篇(43.1%)。根据CONSORT和STRICTA评价结果,报告率较高的条目有“研究目的和假设”“结局指标结果”“针刺穴位”等,报告率较低的条目有“随机”“盲法”“样本量的估算”“临床注册”以及“治疗师背景”“针刺细节”等。没有文献完全符合CONSORT声明和STRICTA清单。评价的一致性较好。结论:目前针刺治疗COPD随机对照试验的偏倚风险较高,报告质量整体较低,需要按照CONSORT和STRICTA进一步完善,以提高临床证据质量,使其更好的服务于临床。

关键词：慢性阻塞性肺疾病针刺 CONSORT标准 STRICTA标准

甘蓝型油菜和甘蓝CRISPR/Cas9编辑效果的快速检测

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中国油料作物学报 2018年第6期40卷 737-744页

作者：孙勤富刘东晓林俐吴德伟吴健王幼平扬州大学生物科学与技术学院江苏扬州225009

CRISPR/Cas9系统是目前最常用的基因组定点编辑工具,通过瞬时表达试验提前验证Cas9/sgRNA载体诱导的突变效率,可以提高基因组定点编辑的成功率,且显著节省费用及时间。本研究开发了一种利用农杆菌介导的操作简单、适用性好、成本低廉的... 详细信息

CRISPR/Cas9系统是目前最常用的基因组定点编辑工具,通过瞬时表达试验提前验证Cas9/sgRNA载体诱导的突变效率,可以提高基因组定点编辑的成功率,且显著节省费用及时间。本研究开发了一种利用农杆菌介导的操作简单、适用性好、成本低廉的叶片瞬时表达技术,可用于快速检测甘蓝型油菜和甘蓝中CRISPR/Cas9的编辑效果。针对甘蓝型油菜Bna C. WRKY11. a基因设计了2个靶位点Tgt1(Target 1)和Tgt2(Target 2),并构建了Cas9/sgRNA-Tgt1/2多重突变载体,在甘蓝型油菜叶片中瞬时表达后,2个靶位点都出现突变,突变效率达11. 2%～82. 2%。针对甘蓝Bol PDS3基因设计了1个靶位点Tgt3(Target3),并构建了Cas9/sgRNA-Tgt3敲除载体,在甘蓝叶片瞬时表达后,Tgt3发生了突变,且大部分为碱基缺失突变,缺失的数目为1～18bp不等,同时还存在少量碱基插入以及碱基替换等突变类型。结果表明这种甘蓝型油菜和甘蓝的叶片瞬时表达技术操作简单、适用性好、成本低廉,可用于CRISPR/Cas9的编辑效果快速检测。

关键词： CRISPR/Cas9 甘蓝型油菜甘蓝基因组编辑突变检测

Optimization of Hydrolysis Conditions for the Isolation of Angiotensin-I Converting Enzyme(ACE) Inhibitory Peptides from Rhopilema hispidum

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Journal of Ocean University of China 2018年第6期17卷 1458-1464页

作者： sun Zhenliang qin Huanlong CAO Duo YAN Xuebing LI Hao HUANG Linsheng QU Xiao KONG Cheng WANG Man Department of General Surgery Shanghai Tenth People's Hospital Tongji University School of Medicine Shanghai Jiaotong University Affiliated Sixth People's Hospital South Campus The College of Life Science Northwest University

To obtain the maximum angiotensin-I converting enzyme(ACE) inhibitory activity, the protein hydrolysis conditions of the jellyfish Rhopilema hispidum were optimized using response surface methodology(RSM). Trypsin was selected to produce R. hispidum protein hydrolysates(RPH) with ACE inhibitory activity. The optimal parameters for producing protein hydrolysates with the highest ACE inhibitory activity were as follows: hydrolysis time 5 h, hydrolysis temperature 50℃, and the enzyme-to-substrate ratio 6%. Under these conditions, the ACE inhibitory rate of RPH could reach 64.28% ± 5.72%. In addition, RPH contained high levels of Gly, Glu, Pro, Ala, Asp and Arg, with a molecular weight distribution range of 0.32–6.84 kDa. The following three novel ACE inhibitory peptides were isolated and identified: Ile-Gly-Glu-Thr-Gly-Pro, Gly-Ala-Thr-Gly-Pro-Ala-Gly-Tyr-Val and Gly-AlaPhe-Gly-Pro-Gly-Gly-Leu-Val-Gly-Arg-Pro. The IC_(50) values of the ACE inhibitory activity of these three purified peptides were 19.07, 27.42 and 31.26 μmol L^(-1), respectively. These results suggested that proteins and peptides isolated from R. hispidum could be utilized as antihypertensive functional food sources.

关键词： Rhopilema hispidum ACE inhibitory peptide response surface methodology protein hydrolysate

Tailoring synergetic catalytic interface of VPO/Ni_(2)P to boost hydrogen evolution under alkaline conditions

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Journal of Energy Chemistry 2022年第2期31卷 674-680页

作者： Wenli Xu Qiqi Li Wenda Zhong Bing sun Qiang Huang Xu Nan Yinhong Gao Yao Yang qin Zhang Nianjun Yang Xuanke Li School of Chemistry and Chemical Engineering Wuhan University of Science and TechnologyWuhan 430081HubeiChina Hunan Province Key Laboratory for Advanced Carbon Materials and Applied Technology Hunan UniversityChangsha 410082HunanChina Institute of Materials Engineering University of SiegenSiegen 57076Germany

Design of the catalyst for efficient water dissociation and hydrogen recombination is paramount in enhancement of the alkaline water electrolysis ***,we reported a delicate hierarchical(VO)_(2) P_(2)O_(7)-Ni_(2) P@NF(VPO-Ni_(2) P@NF)hybrid catalyst that operated efficiently in alkaline *** VPO and Ni_(2) P respectively act as the water dissociation promoter and the hydrogen recombination center,which synergistically propel water adsorption/dissociation and H intermediates *** resulting synergistic interfaces between VPO and Ni_(2) P are verified to afford the catalyst an outstanding performance for hydrogen evolution reaction in alkaline media with an overpotential of 154 mV at 10 mA cm^(-2),Tafel slope of 65 mV dec^(-1),and remarkable ***,the catalyst presents the potential for overall water *** work may shed fresh light on the high-performance electrocatalyst design and the application of VPO on water electrolysis.

关键词： Alkaline water electrolysis Interface engineering (VO)_(2)P_(2)O_(7) Ni_(2)P