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2013年（第三届）中国药物毒理学年会暨药物非临床安全性评价研究论坛

作者： sun Jian-hua WANG Ying sun xiao-xun HUANG Gui-hua GAO Jing-li QI Qiu-ping Center for Drug Safety Evaluation and Research Shanghai Institute of Materia MedicaChinese Academy of Sciences

OBJECTIVE To validate a flow-cytometry-based method for the measurement of lymphocyte subgroups in cynomolgus monkeys and Sprague Dawley rats,*** For cynomolgus monkeys,two panels of fluorescent antibodies,namely CD3-FITC/CD4-PerCP/CD8-PE and CD3-PE-Cy5.5/CD16-PE/CD20-FITC were used to analyze the proprotion of T cells including T helper cell(CD3 CD4) and T cytotoxic cells(CD3 CD8),NK cells(CD3 CD16) and B cells(CD3 CD20).For Sprague Dawley rats,other two panels of fluorescent antibodies,namely CD3 FITC/CD4-PE/CD8a-PerCP and CD3 FITC/CD45RA-PE-Cy5.5/CD161a-PE were used to analyze the proprotion of T cells,NK cells(CD3CD161) and B cells(CD3CD45RA).Blood samples from 10 monkeys and 10 rats were analyzed for intra-assay precision,sample stability and inter-subject ***,intra-animal precision was investigated in *** For cynomolgus monkeys,intra-assay CV and intra-animal CV for all parameters were within 15%.The positive binding signals of all isotype controls were less than 1%.Furthermore,monkey blood samples were stable for all parameters when kept at room temperature for 6 h or at 4℃for 1 *** after fixation were stable while stored in the refrigerator(+4℃) for 24 *** rats,intra-assay CVs for all parameters were within 15%.The positive binding signals of isotype controls of CD3/CD4 and CD3/CD8 were less than 1%.Furthermore,rat blood samples were stable for CD3/CD4 and CD3/CD8 while stored in the refrigerator(+4℃) for 24 ***,rat blood samples were unstable for CD3/161 a when kept at room temperature for 6h and unstable for CD3/45RA when kept at 4℃for 1 *** latter two cases,the positive binding signals of the isotype controls were around 2%.CONCLUSION All the parameters tested fulfilled the criteria of acceptance with a few minor exceptions related to stability data from rat blood samples. It is suitable for the measurement of lymphocyte subgroups in cynomolgus monkeys and Sprague Dawley rats.

关键词： lymphocyte phenotype flow cytometry

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Method validation of lymphocyte subgroup phenotyping analysis in cynomolgus monkeys and Sprague-Dawley rats using flow cytometry

Method validation of lymphocyte subgroup phenotyping analysi...

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作者： sun Jian-hua WANG Ying sun xiao-xun HUANG Gui-hua GAO Jing-li QI Qiu-ping Center for Drug Safety Evaluation and Research Shanghai Institute of Materia MedicaChinese Academy of Sciences

OBJECTIVE To validate a flow-cytometry-based method for the measurement of lymphocyte subgroups in cynomolgus monkeys and Sprague Dawley rats,*** For cynomolgus monkeys,two panels of fluorescent antibodies,namely CD3-FITC/CD4-PerCP/CD8-PE and CD3-PE-Cy5.5/CD16-PE/CD20-FITC were used to analyze the proprotion of T cells including T helper cell(CD3~+CD4~+) and T cytotoxic cells(CD3~+CD8~+),NK cells(CD3~- CD16~+) and B cells(CD3~- CD20~+).For Sprague Dawley rats,other two panels of fluorescent antibodies,namely CD3~- FITC/CD4-PE/CD8a-PerCP and CD3-FITC/CD45RA-PE-Cy5.5/CD161a-PE were used to analyze the proprotion of T cells,NK cells(CD3~-CD161~+) and B cells(CD3~-CD45RA~+).Blood samples from 10 monkeys and 10 rats were analyzed for intra-assay precision,sample stability and inter-subject ***,intra-animal precision was investigated in *** For cynomolgus monkeys,intra-assay CV and intra-animal CV for all parameters were within 15%.The positive binding signals of all isotype controls were less than 1%.Furthermore,monkey blood samples were stable for all parameters when kept at room temperature for 6 h or at 4℃for 1 *** after fixation were stable while stored in the refrigerator(+4℃) for 24 *** rats,intra-assay CVs for all parameters were within 15%.The positive binding signals of isotype controls of CD3~+/CD4~+ and CD3~+/CD8~+ were less than 1%.Furthermore,rat blood samples were stable for CD3~+/CD4~+ and CD3~+/CD8~+ while stored in the refrigerator(+4℃) for 24 ***,rat blood samples were unstable for CD3/161a when kept at room temperature for 6h and unstable for CD3/45RA when kept at 4℃for 1 *** latter two cases,the positive binding signals of the isotype controls were around 2%.CONCLUSION All the parameters tested fulfilled the criteria of acceptance with a few minor exceptions related to stability data from rat blood samples. It is suitable for the measurement of lymphocyte subgroups in cynomolgus monkeys and S

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Method validation of lymphocyte subgroup phenotyping analysis in cynomolgus monkeys and Sprague-Dawley rats using flow cytometry

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中国药理学与毒理学杂志 2013年第3期 513-513页

作者： sun Jian-hua WANG Ying sun xiao-xun HUANG Gui-hua GAO Jing-li QI Qiu-ping Center for Drug Safety Evaluation and Research Shanghai Institute of Materia MedicaChinese Academy of Sciences

OBJECTIVE To validate a flow-cytometry-based method for the measurement of lymphocyte subgroups in cynomolgus monkeys and Sprague Dawley rats,*** For cynomolgus monkeys,two panels of fluorescent antibodies,namely CD3-FITC /CD4-PerCP /CD8-PE and CD3-PE-Cy5.5 /CD16-PE /CD20-FITC were used to analyze the proprotion of T cells including T helper cell(CD3 + CD4 +) and T cytotoxic cells(CD3 + CD8 +),NK cells(CD3 CD16 +) and B cells(CD3-CD20 +).For Sprague Dawley rats,other two panels of fluorescent antibodies,namely CD3 FITC /CD4-PE /CD8a-PerCP and CD3-FITC /CD45RA-PE-Cy5.5 /CD161a-PE were used to analyze the proprotion of T cells,NK cells(CD3-CD161 +) and B cells(CD3-CD45RA +).Blood samples from 10 monkeys and 10 rats were analyzed for intra-assay precision,sample stability and inter-subject ***,intra-animal precision was investigated in *** For cynomolgus monkeys,intra-assay CV and intra-animal CV for all parameters were within 15%.The positive binding signals of all isotype controls were less than 1%.Furthermore,monkey blood samples were stable for all parameters when kept at room temperature for 6 h or at 4℃ for 1 *** after fixation were stable while stored in the refrigerator(+ 4℃) for 24 *** rats,intra-assay CVs for all parameters were within 15%.The positive binding signals of isotype controls of CD3 + /CD4 + and CD3 + /CD8 + were less than 1%.Furthermore,rat blood samples were stable for CD3 + /CD4 + and CD3 + /CD8 + while stored in the refrigerator(+ 4℃) for 24 ***,rat blood samples were unstable for CD3 /161a when kept at room temperature for 6h and unstable for CD3 /45RA when kept at 4℃ for 1 *** latter two cases,the positive binding signals of the isotype controls were around 2%.CONCLUSION All the parameters tested fulfilled the criteria of acceptance with a few minor exceptions related to stability data from rat blood *** is suitable for the measurement of lymphocyte subgroups in cynomolgus mo

关键词： lymphocyte phenotype flow cytometry

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miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu Yan Yu xiao-Li Li xiao-Ting sun Li-Ting Wang Xia Wu Ling-Jie Zhu xiao-Jia Yang Qian-Ru He Fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

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Electrode/Electrolyte Optimization‑Induced Double‑Layered Architecture for High‑Performance Aqueous Zinc‑(Dual)Halogen Batteries

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Nano-Micro Letters 2025年第3期17卷 121-137页

作者： Chengwang Zhou Zhezheng Ding Shengzhe Ying Hao Jiang Yan Wang Timing Fang You Zhang Bing sun xiao Tang xiaomin Liu School of Textiles and Clothing School of Chemistry and Chemical EngineeringQingdao UniversityQingdao 266071ShandongPeople’s Republic of China Centre for Clean Energy Technology School of Mathematical and Physical SciencesFaculty of ScienceUniversity of Technology SydneyUltimoNSW 2007Australia School of Chemistry and Chemical Engineering Henan Normal UniversityXinxiang 453007HenanPeople’s Republic of China

Aqueous zinc-halogen batteries are promising candidates for large-scale energy storage due to their abundant resources,intrinsic safety,and high theoretical ***,the uncontrollable zinc dendrite growth and spontaneous shuttle effect of active species have prohibited their practical ***,a double-layered protective film based on zinc-ethylenediamine tetramethylene phosphonic acid(ZEA)artificial film and ZnF2-rich solid electrolyte interphase(SEI)layer has been successfully fabricated on the zinc metal anode via electrode/electrolyte synergistic *** ZEA-based artificial film shows strong affinity for the ZnF2-rich SEI layer,therefore effectively suppressing the SEI breakage and facilitating the construction of double-layered protective film on the zinc metal *** double-layered architecture not only modulates Zn2+flux and suppresses the zinc dendrite growth,but also blocks the direct contact between the metal anode and electrolyte,thus mitigating the corrosion from the active *** employing optimized metal anodes and electrolytes,the as-developed zinc-(dual)halogen batteries present high areal capacity and satisfactory cycling *** work provides a new avenue for developing aqueous zinc-(dual)halogen batteries.

关键词： Zn metal anodes Double-layered protective film Electrode/electrolyte optimization Aqueous zinc-(dual)halogen batteries

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Metal nanoparticles decorated CoFe-(oxy)hydroxysulfides nanosheets fabricated by a general strategy for electrocatalytic water splitting

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Journal of Energy Chemistry 2025年第1期100卷 26-38页

作者： xiaodong Yang Haochen Shen xiaoming xiao Zhichao Li Qi Zhou Wei Yang Bin Jiang Yongli sun Luhong Zhang Zhenhua Yan School of Chemical Engineering and Technology Tianjin University Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Nankai University

This study presents a novel method to fabricate metal-decorated,sulfur-doped layered double hydroxides （M/SLDH） through spontaneous redox and sulfurization *** developed Ag/SLDH and Pt/SLDH catalysts with abundant heterogeneous interfaces and hierarchical nanostructures demonstrated outstanding oxygen evolution reaction （OER） and hydrogen evolution reaction （HER） performance,achieving low overpotentials of 212 and 35 mV at 10 mA cm-2in 1 M KOH,*** both anode and cathode in water splitting,they required only 1.47 V to reach 10 mA cm-2and exhibited high structural robustness,maintaining stability at 1000 mA cm-2for 300 ***-situ Raman analysis revealed that the synergistic effects of metal nanoparticles and S doping significantly promote the transformation into the S-Co1-xFexOOH layer,which serves as the active phase for water ***,ultraviolet photoelectron spectroscopy （UPS） and density functional theory （DFT） analyses indicated that incorporating metal nanoparticles and S doping increase electron density near the Fermi level and reduce reaction energy barriers,thus enhancing intrinsic OER and HER *** study provides a scalable strategy for synthesizing high-performance electrocatalysts for water splitting,with promising potential for broader applications.

关键词： Ultraviolet photoelectron spectroscopy

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Recent Advances in Fibrous Materials for Hydroelectricity Generation

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Nano-Micro Letters 2025年第2期17卷 109-133页

作者： Can Ge Duo Xu xiao Feng Xing Yang Zheheng Song Yuhang Song Jingyu Chen Yingcun Liu Chong Gao Yong Du Zhe sun Weilin Xu Jian Fang College of Textile and Clothing Engineering Soochow UniversitySuzhou 215123People’s Republic of China National Engineering Laboratory for Modern Silk Soochow UniversitySuzhou 215123People’s Republic of China State Key Laboratory of New Textile Materials and Advanced Processing Technologies Wuhan Textile UniversityWuhan 430200People’s Republic of China Institute of Functional Nano&Soft Materials(FUNSOM) Soochow UniversitySuzhou 215123People’s Republic of China Department of Electrical and Computer Engineering University of MassachusettsAmherstMA 01003USA Department of Materials Imperial College LondonSouth Kensington CampusLondon SW72AZUK College of Textile Science and Engineering Zhejiang Sci-Tech UniversityHangzhou 310018People’s Republic of China School of Materials Science and Engineering Shanghai Institute of Technology100 Haiquan RoadShanghai 201418People’s Republic of China

Depleting fossil energy sources and conventional polluting power generation pose a threat to sustainable *** generation from ubiquitous and spontaneous phase transitions between liquid and gaseous water has been considered a promising strategy for mitigating the energy *** materials with unique flexibility,processability,multifunctionality,and practicability have been widely applied for fibrous materials-based hydroelectricity generation(FHG).In this review,the power generation mechanisms,design principles,and electricity enhancement factors of FHG are first ***,the fabrication strategies and characteristics of varied constructions including 1D fiber,1D yarn,2D fabric,2D membrane,3D fibrous framework,and 3D fibrous gel are ***,the advanced functions of FHG during water harvesting,proton dissociation,ion separation,and charge accumulation processes are analyzed in ***,the potential applications including power supply,energy storage,electrical sensor,and information expression are also ***,some existing challenges are considered and prospects for future development are sincerely proposed.

关键词： Hydroelectricity Fibrous material Streaming potential Ion diffusion

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北细辛提取物对二斑叶螨的毒性

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江苏农业学报 2011年第5期27卷 1007-1010页

作者：韩俊艳王军孙晓逊纪明山沈阳农业大学植物保护学院辽宁沈阳110866 沈阳大学生物与环境工程学院辽宁沈阳110044

分别采用玻片浸渍法和叶片残毒法测定了北细辛不同溶剂提取物对二斑叶螨雌成螨和卵的触杀活性,采用生化方法测定了高活性提取物作用下雌成螨谷胱甘肽巯基转移酶(GSTs)和Na+,K+-ATP酶的活性。结果表明:北细辛甲醇提取物杀螨作用最强,经1 ... 详细信息

分别采用玻片浸渍法和叶片残毒法测定了北细辛不同溶剂提取物对二斑叶螨雌成螨和卵的触杀活性,采用生化方法测定了高活性提取物作用下雌成螨谷胱甘肽巯基转移酶(GSTs)和Na+,K+-ATP酶的活性。结果表明:北细辛甲醇提取物杀螨作用最强,经1 mg/ml提取物处理24 h后,雌成螨与卵的校正死亡率分别为66.5%与45.7%,致死中浓度(LC50)分别为0.57 mg/ml和0.88 mg/ml;1/2 LC50浓度的甲醇提取物可使雌成螨GSTs活性升高,同时抑制雌成螨Na+,K+-ATP酶的活性。说明:北细辛甲醇提取物对二斑叶螨雌成螨和卵具有触杀活性,作用机制可能与抑制Na+,K+-ATP酶的活性有关。

关键词：北细辛二斑叶螨杀螨剂谷胱甘肽巯基转移酶

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Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying sun Haoyan Li Dongqing sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing Jun Wu Minmin xiao Fangqing Zhao Jing-Dong J.Han Qi Liu xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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Joint Feature Encoding and Task Alignment Mechanism for Emotion-Cause Pair Extraction

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Computers, Materials & Continua 2025年第1期82卷 1069-1086页

作者： Shi Li Didi sun College of Computer and Control Engineering Northeast Forestry UniversityHarbin150040China

With the rapid expansion of social media,analyzing emotions and their causes in texts has gained significant ***-cause pair extraction enables the identification of causal relationships between emotions and their triggers within a text,facilitating a deeper understanding of expressed sentiments and their underlying *** comprehension is crucial for making informed strategic decisions in various business and societal ***,recent research approaches employing multi-task learning frameworks for modeling often face challenges such as the inability to simultaneouslymodel extracted features and their interactions,or inconsistencies in label prediction between emotion-cause pair extraction and independent assistant tasks like emotion and cause *** address these issues,this study proposes an emotion-cause pair extraction methodology that incorporates joint feature encoding and task alignment *** model consists of two primary components:First,joint feature encoding simultaneously generates features for emotion-cause pairs and clauses,enhancing feature interactions between emotion clauses,cause clauses,and emotion-cause ***,the task alignment technique is applied to reduce the labeling distance between emotion-cause pair extraction and the two assistant tasks,capturing deep semantic information interactions among *** proposed method is evaluated on a Chinese benchmark corpus using 10-fold cross-validation,assessing key performance metrics such as precision,recall,and F1 *** results demonstrate that the model achieves an F1 score of 76.05%,surpassing the state-of-the-art by 1.03%.The proposed model exhibits significant improvements in emotion-cause pair extraction(ECPE)and cause extraction(CE)compared to existing methods,validating its *** research introduces a novel approach based on joint feature encoding and task alignment mechanisms,contributing to advancements in emotion-c

关键词： Emotion-cause pair extraction interactive information enhancement joint feature encoding label consistency task alignment mechanisms

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