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A prokaryotic mycoplasma-like organism infection in the scallop Argopecten irradians

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Acta Oceanologica Sinica 2007年第2期26卷 150-156页

作者： ZHANG Weizhu WU Xinzhong sun jingfeng LI Dengfeng Biology Department Chuzhou College Chuzhou 239000 China College of Animal Sciences Zhejiang University Hangzhou 310029 China South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China

Intracytoplasmic infection with a prokaryotic mycoplasma-like organism （MLO） were characterized in the scallop Argopecten irradians, and the first report of such infection in this scallop was represented. Ultrastructurally these microorganisms are usually pleomorphic and variable in morphology and size, and appear in several cell types such as small spherical body, rod-shaped bodies and longer filament-shaped body. They lack a cell wall. These observations revealed that these types of the prokaryote showed some characteristics of a mycoplasma-like organism （MLO）. MLO reproduced in two ways： binary fission and budding. The results of isolation and purification showed that a large number of MLOs existed in the tissues of diseased scallops. The results of experimental infection revealed that the MLO is pathogenic to the scallop Argopecten irradians.

关键词： Argopecten irradians mycoplasma-like organism purification experimental infection

凡纳滨对虾肠道中地衣芽孢杆菌的分离鉴定及其生物学特性

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大连海洋大学学报 2024年第1期39卷 74-82页

作者：徐振权韩国威任静张子轩孙敬锋韩卓然天津农学院水产学院天津市水产生态及重点实验室天津300384

为筛选对凡纳滨对虾(Litopenaeus vannamei)具有潜在益生效果的菌株,从体质量为(3.1±0.4)g的健康凡纳滨对虾肠道中筛选出一株具有耐高温(80℃)能力的菌株(命名为RHVJ),采用形态学观察、生理生化鉴定、基因序列比对、病原菌拮抗能力测... 详细信息

为筛选对凡纳滨对虾(Litopenaeus vannamei)具有潜在益生效果的菌株,从体质量为(3.1±0.4)g的健康凡纳滨对虾肠道中筛选出一株具有耐高温(80℃)能力的菌株(命名为RHVJ),采用形态学观察、生理生化鉴定、基因序列比对、病原菌拮抗能力测定、安全性与耐受性分析和生长特性检测等方法对其展开研究。结果表明:菌株RHVJ为革兰氏阳性杆菌,综合其生理生化特征可初步鉴定该菌属于芽孢杆菌属(Bacillus);菌株RHVJ的16S rDNA序列与地衣芽孢杆菌(Bacillus licheniformis)的MH305331.1和NR074923.1完全匹配,其gyrB序列与CP038186.1和CP023729.1也达到了100%的一致性;综合菌体形态、生理生化及分子生物学特征,判定菌株RHVJ为地衣芽孢杆菌;地衣芽孢杆菌RHVJ可抑制副溶血弧菌(Vibrio parahaemolyticus)和维氏气单胞菌(Aeromonas veronii)生长,对凡纳滨对虾无致病性;在胆盐质量浓度为0~1.2 g/L、pH为3~11环境下培养,以及在37~90℃下进行5min或15 min水浴处理后,菌株RHVJ仍可持续增殖,该菌在27℃、pH 7和盐度20条件下生长最佳。研究表明,凡纳滨对虾肠道源地衣芽孢杆菌RHVJ环境适应性良好且安全,具有一定的病原菌拮抗能力,可作为候选菌株开发为益生菌制剂。

关键词：凡纳滨对虾肠道地衣芽孢杆菌益生菌

In situ simultaneous determination of trace elements,U-Pb and Lu-Hf isotopes in zircon and baddeleyite

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Chinese Science Bulletin 2008年第10期53卷 1565-1573页

作者： XIE LieWen ZHANG YanBin ZHANG HuiHuang sun jingfeng WU FuYuan State Key Laboratory of Lithospheric Evolution Institute of Geology and Geophysics Chinese Academy of Sciences Beijing 100029China

This paper describes a combined method of simultaneously measuring U-Pb and Lu-Hf isotopes as well as trace elements in Phalaborwa baddeleyite and 91500, GJ-1, TEMORA-1 and SK10-2 zircons by means of Neptune MC-ICPMS and Agilent Q-ICPMS connected to a 193 nm excimer laser ablation system. Material ablated by laser was carried in different proportions into Q-ICPMS for U-Pb isotopic and trace elemental and MC-ICPMS for Lu-Hf isotopic compositions. Experiments indicate that different proportions of ablated material for the Q-ICPMS and MC-ICPMS (6:4, 5:5 and 4:6 respectively) do not show any bias for the zircon/baddeleyite U-Pb age, Lu-Hf isotope and trace elemental compositions within ana-lytical errors. Using 40―60 μm spot size, the obtained U-Pb ages of Phalaborwa baddeleyite, 91500, GJ-1, TEMORA and SK10-2 zircons are 2065±15 (2σ, n=20), 1063±6 (2σ, n=19), 613±6 (2σ, n=20), 416±5 (2σ, n=20) and 32.6±0.5 (2σ, n=20) Ma, respectively. The 176Hf/177Hf ratios are 0.281231±24 (2SD, n=20), 0.282310±35 (2SD, n=19), 0.282028±34 (2SD, n=20), 0.282687±34 (2SD, n=20) and 0.282752±53 (2SD, n=20), respectively. The obtained trace elemental compositions are identical to the reference values. Therefore, this kind of technique makes it possible to simultaneously obtain the U-Pb age, Lu-Hf iso-topes and trace elemental compositions of zircon and baddeleyite, which could be an important tool in solving problems in earth sciences.

关键词：镥-铪同位素锆石年代测量地球化学

克里金方法与深度学习方法用于浅层地下水位估计的对比研究:以深汕特别合作区为例

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地质科技通报 2024年第4期43卷 291-301页

作者：汪泉娟孙敬锋杨英杰张将伟王国梁马恩泽刘进赵鑫深圳市生态环境智能管控中心广东深圳518000 南方科技大学环境科学与工程学院广东深圳518000 深圳市广汇源环境水务有限公司广东深圳518000

掌握区域地下水位分布是地下水资源评价与环境保护的重要基础。由于区域尺度观测的地下水位数据有限,克里金插值与深度学习方法逐渐被用于区域地下水位预测,但两者的适用性及鲁棒性缺乏对比分析。针对这个问题,基于239口监测井水位,采... 详细信息

掌握区域地下水位分布是地下水资源评价与环境保护的重要基础。由于区域尺度观测的地下水位数据有限,克里金插值与深度学习方法逐渐被用于区域地下水位预测,但两者的适用性及鲁棒性缺乏对比分析。针对这个问题,基于239口监测井水位,采用普通克里金方法、融合地表高程的协同克里金方法、深度学习方法估计深汕特别合作区地下水位空间分布,调查3种方法在区域地下水位预测中的应用潜力。为了研究训练集样本量对3种方法预测效果的影响,将239口监测井分为2组(76口和163口)用于3种模型的训练。结果显示,使用76眼井的训练数据拟合验证集样本时RMSE分别为6.09,4.04,7.11,考虑了地表高程信息的协同克里金法明显优于普通克里金法与深度学习法。而当训练样本量增加到163口水位数据时,普通克里金、协同克里金及深度学习法的预测精度都明显提升,3种方法拟合验证数据集的RMSE相差很小,分别为1.33,1.36,1.54。另外,使用较大数据样本量进行全区域的地下水位预测时不同方法得到的预测水位分布均有所提高,但空间分布特征依旧存在明显差异。结果表明,当观测数据稀疏时,融合高程信息的协同克里金方法的预测效果显著高于普通克里金方法和深度学习方法,而当观测数据量增加到达一定数量时,3种方法预测得到的RMSE较接近。

关键词：地下水位克里金方法深度学习方法深汕特别合作区

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Design and validation of a variable camber wing structure

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Chinese Journal of Aeronautics 2024年第2期37卷 1-11页

作者： Xiasheng sun jingfeng XUE Jin ZHOU Zhigang WANG Wenjuan WANG Mengjie ZHANG Chinese Aeronautical Establishment Beijing 100012China School of Aeronautic Science and Engineering Beihang UniversityBeijing 100191China Aircraft Strength Research Institute of China Xi’an 710065China Graduate School of Chinese Aeronautical Establishment Yangzhou 225002China

Variable camber wing technology is one of the important development trends of green aviation at *** smooth,seamless,continuous and adaptive change of wing camber,the aerodynamic performance is improved in achieving increase in lift and reduction in resistance and *** on the aerodynamic validation model CAE-AVM,Chinese Aeronautical Establishment(CAE)has carried out the design and validation of a variable camber wing,proposed an aerodynamic deformation matrix for the leading and trailing edges of aircraft wings in takeoff,landing and cruise *** structures and driving schemes are compared,and several key technology problems of leading and trailing edge deformation are solved.A full-size leading edge wind tunnel test piece with a span of 2.7 m and a trailing edge ground function test piece are *** deformation and shape maintenance capabilities of the leading edge is verified under real wind load conditions,and the load bearing and deformation capabilities of the trailing edge is verified under simulated follow-on *** results indicate that the leading and trailing edges of the variable camber wing can achieve the required deformation angle and have a certain load-bearing *** study can provide some insights into the application of variable camber wing technology for civil aircraft.

关键词： Variable camber wing Leading edge Trailing edge Structure Measurement and control Test

香港牡蛎感染才女虫后外套膜与鳃的转录组测序及差异表达基因分析

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天津科技 2024年第8期51卷 37-44页

作者：郭长钰王江勇孙敬锋袁凯赵嘉豪天津农学院水产学院天津300384 惠州学院生命与科学学院广东惠州516007

为探明香港牡蛎应对才女虫感染的免疫应答机制,利用Illumina技术,比较才女虫感染前后的香港牡蛎外套膜和鳃的转录组,注释牡蛎感染才女虫后产生的差异表达基因,并验证免疫代谢应答的相关基因,共获得74.85 GB纯净数据,其中:感染的外套膜有... 详细信息

为探明香港牡蛎应对才女虫感染的免疫应答机制,利用Illumina技术,比较才女虫感染前后的香港牡蛎外套膜和鳃的转录组,注释牡蛎感染才女虫后产生的差异表达基因,并验证免疫代谢应答的相关基因,共获得74.85 GB纯净数据,其中:感染的外套膜有6 923个差异表达基因,上调基因3 770个,下调基因3 153个;感染的鳃有7 410个差异表达基因,上调基因3646个,下调基因3764个;受感染与健康牡蛎外套膜和鳃差异组分别有4398和4780个基因注释到GO分类上;外套膜和鳃转录组分别有3 851和4 367个差异表达基因注释在KEGG数据库中,大多数富集到的通路与机体的免疫相关。挑选5个差异表达基因进行荧光定量验证,包括热休克蛋白70(HSP70)、42同系物(CDC42)、细胞色素P450(CYP450)等在内的免疫代谢相关基因,结果表明,所挑选差异基因的表达趋势与高通分量测序结果基本一致,可通过加强体内多种免疫代谢基因协同作用来抵抗才女虫感染。

关键词：香港牡蛎才女虫感染差异表达基因转录组外套膜鳃

Transfer-free chemical vapor deposition graphene for nitride epitaxy: challenges, current status and future outlook

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Science China Chemistry 2024年第3期67卷 824-840页

作者： Xiang Gao Senlin Li jingfeng Bi Kaixuan Zhou Meng Li Zhongfan Liu Jingyu sun College of Energy Soochow Institute for Energy and Materials InnovationsSUDA-BGI Collaborative Innovation CenterKey Laboratory of Advanced Carbon Materials and Wearable Energy Technologies of Jiangsu ProvinceSoochow UniversitySuzhou 215006China Beijing Graphene Institute Beijing 100095China Xiamen Silan Advanced Compound Semiconductor Co.Ltd. Xiamen 361026China

Graphene, a two-dimensional material with outstanding electrical and mechanical properties, has attracted considerable attention in the field of semiconductor technologies due to its potential use as a buffer layer for the epitaxial Ⅲ-nitride growth. In recent years, significant progress has been made in the chemical vapor deposition growth of graphene on various insulating substrates for the nitride epitaxy, which offers a facile, inexpensive, and easily scalable methodology. However, certain challenges are still present in the form of producing high-quality graphene and achieving optimal interface compatibility with Ⅲ-nitride *** this review, we provide an overview of the bottlenecks associated with the transferred graphene fabrication techniques and the state-of-the-art techniques for the transfer-free graphene growth. The present contribution highlights the current progress in the transfer-free graphene growth on different insulating substrates, including sapphire, quartz, SiO_(2)/Si, and discusses the potential applications of transfer-free graphene in the Ⅲ-nitride epitaxy. Finally, it includes the prospects of the transfer-free graphene growth for the Ⅲ-nitride epitaxy and the challenges that should be overcome to realize its full potential in this field.

关键词： Transfer-free chemical vapor deposition graphene for nitride epitaxy challenges current status and future outlook graphene

Total body irradiation of donors can alter the course of tolerance and induce acute rejection in a spontaneous tolerance rat liver transplantation model

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Science China(Life Sciences) 2012年第9期55卷 774-781页

作者： ZHANG YeWei ZHAO HeWei BO Lin YANG YinXue LU Xiang sun jingfeng WEN JianFei HE Xia YIN GuoWen Department of Hepatobiliary and Pancreatic Surgery Affiliated Jiangsu Cancer Hospital of Nanjing Medical UniversityNanjing 210009China Department of Rheumatism and Immunology Affiliated No.2 Hospital of Suzhou UniversitySuzhou 215000China Department ofGeneral Surgery Affiliated Hospital of Ningxia Medical UniversityYinchuan 750004China Department of Geriatrics The Second Affiliated Hospital of Nanjing Medical UniversityNanjing 210011China Department of Radiotherapy Affiliated Jiangsu Cancer Hospital of Nanjing Medical UniversityNanjing 210009China Department of lnterventional Affiliated Jiangsu Cancer Hospital of Nanjing Medical UniversityNanjing 210009China

Liver transplantation is an established therapy for end-stage liver diseases. Graft rejection occurs unless the recipient receives immunosuppression after transplantation. This study aimed to explore the mechanism of acute rejection of liver allografts in rats pre-treated with total body irradiation to eliminate passenger lymphocytes and to define the role of CD4＋CD25＋ regulatory T cells in the induction of immunotolerance in the recipient. Male Lewis rats were used as donors and male DA rats were re- cipients. Rats were randomly assigned to the following four groups： control group, homogeneity liver transplantation group, idio-immunotolerance group and acute rejection group. After transplantation, the survival time of each group, serum alanine aminotransferase, total bilirubin levels, number of Foxp3＋CD4＋CD25＋ regulatory T cells, expression of glucocorticoid-induced tumor necrosis factor receptor on T cell subgroups, histopathology of the hepatic graft and spleen cytotoxic T lymphocyte lytic activity were measured. In the acute rejection group, where donors were preconditioned with total body in＇adiation before liver transplantation, all recipients died between day 17 and day 21. On day 14, serum alanine aminotransferase increased signifi- cantly to （459.2±76.9） U L^- 1, total bilirubin increased to （124.1±33.7） μmol L-1 （P〈0.05） and the ratio of Foxp3＋CD4＋CD25＋ regulatory T cells decreased significantly to 1.50%±0.50% （P〈0.05） compared with the other groups. Analysis of the T cell subpopulations in the acute rejection group varied from the other groups. Histological analysis showed typical changes of acute rejection in the acute rejection group only. Preconditioning of the donors with total body irradiation eliminated passenger lymphocytes of the liver graft, and thus affected the course of tolerance and induced acute rejection after liver transplantation.

关键词： liver transplantation immune tolerance Foxp3+ regulatory T cell GITR

Effects of Epstein-Barr virus infection on the development of multiple myeloma after liver transplantation

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Science China(Life Sciences) 2012年第8期55卷 735-743页

作者： ZHANG YeWei ZHAO HeWei HE Xia ZHENG SuWen WANG TaiHong YAN DongLiang sun jingfeng LU Xiang WEN JianFei LAU Wan Yee Department of Hepatobiliary and Pancreatic Surgery Affiliated Jiangsu Cancer Hospital of Nanjing Medical UniversityNanjing 210009China Department of Radiotherapy Affiliated Jiangsu Caneer Hospital of Nanjlng Medical University Nanjing 210009China Department of General surgery The Second Affiliated Hospital of Nantong University Nantong 226001China Departimen of Geriatrics The Second Affiliated Hospital of Nanjing Medical UniversityNanjing 210011China Faculty of Medicine The Chinese University of Hong KongPrince of Wales HospitalShatinHong KongChina

Reduced cellular immune function in patients after liver transplantation easily results in many types of viral infections,such as Epstein-Barr ***-Barr virus is a γ-herpesvirus and is related to many malignant diseases,especially epithelial and lymph *** abnormal interaction of cluster of differentiation 40 with cluster of differentiation 40 ligand and expression of cluster of differentiation 40 ligand are considered closely related to the development of myeloma *** study explored the influence and mechanism of Epstein-Barr virus infection on the phenotype and biological behavior of myeloma cells after liver *** cytometry was used to detect coexpression of cluster of differentiation 40 and cluster of differentiation 40 ligand in 10 samples of freshly isolated multiple myeloma *** of differentiation 40 and cluster of differentiation 40 ligand were coexpressed in sample Nos.5,8,9,and 10,particularly in sample *** blot analysis was used to detect the expression of the Epstein-Barr virus antigens latent membrane protein 1 and Epstein-Barr virus nuclear antigen 2 in the multiple myeloma cell line RPMI 8226 infected with Epstein-Barr *** antigen expression indicated that Epstein-Barr virus can infect multiple myeloma virus cells in *** transcription-polymerase chain reaction revealed upregulated expression of cluster of differentiation 40 ligand on the infected RPMI 8226 cells,which may be involved in the anti-apoptosis activity of the infected *** microscopy showed that pairs of molecules of cluster of differentiation 40,cluster of differentiation 40 ligand,and latent membrane protein 1 were colocalized on the surface of the infected *** chemokine receptor 4 was upregulated on the RPMI 8226 cells after Epstein-Barr virus *** migratory ability of the infected cells improved in the presence of the chemokine stromal cell-derived factor-1α.Anti-apoptosis and migration are k

关键词： liver transplantation CD40 CD40L multiple myeloma EBV